Restriction map for
pFN21AB5748
(Circular) (Six-base) MAPSORT of: pFN21AB5748.seq Check: 7695 from: 1 to: 6943
pFN21AB5748 6943 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2903 2903
Size: 6943
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4753 278
Size: 53 83 186 936 3217 2468
Fragments arranged by size:
3217 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2352 2358 4286 1937
Size: 415 6 1928 4594
Fragments arranged by size:
4594 1928 415 6
Acc65I G'GTAC_C
Cuts at: 2814 3777 4264 2814
Size: 963 487 5493
Fragments arranged by size:
5493 963 487
AclI AA'CG_TT
Cuts at: 1563 5070 5443 1563
Size: 3507 373 3063
Fragments arranged by size:
3507 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3022 3132 3240 3950 5006 6390 6658 1632
Size: 1390 110 108 710 1056 1384 268 1917
Fragments arranged by size:
1917 1390 1384 1056 710 268 110 108
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6746
AflIII A'CryG_T
Cuts at: 1203 5842 6819 1203
Size: 4639 977 1327
Fragments arranged by size:
4639 1327 977
AgeI A'CCGG_T
Cuts at: 5774 5774
Size: 6943
AhdI GACnn_n'nnGTC
Cuts at: 2500 5672 2500
Size: 3172 3771
AleI CACnn'nnGTG
Cuts at: 1166 3602 1166
Size: 2436 4507
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6943
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3836 3868 3836
Size: 32 6911
AlwNI CAG_nnn'CTG
Cuts at: 3872 4686 6258 3872
Size: 814 1572 4557
Fragments arranged by size:
4557 1572 814
ApaLI G'TGCA_C
Cuts at: 2237 2516 5000 6156 2237
Size: 279 2484 1156 3024
Fragments arranged by size:
3024 2484 1156 279
ApoI r'AATT_y
Cuts at: 1493 3440 4252 4478 4630 6739 1493
Size: 1947 812 226 152 2109 1697
Fragments arranged by size:
2109 1947 1697 812 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3120 5497 160
Size: 872 2088 2377 1606
Fragments arranged by size:
2377 2088 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6943
AvaI C'yCGr_G
Cuts at: 1870 1943 4065 4268 1870
Size: 73 2122 203 4545
Fragments arranged by size:
4545 2122 203 73
BamHI G'GATC_C
Cuts at: 4273 4640 4273
Size: 367 6576
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2814 3777 4264 5719 618
Size: 325 217 627 1027 963 487 1455 1842
Fragments arranged by size:
1842 1455 1027 963 627 487 325 217
BanII G_rGCy'C
Cuts at: 1391 2426 4262 1391
Size: 1035 1836 4072
Fragments arranged by size:
4072 1836 1035
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5972
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4095 4129 5134 5168 1903
Size: 34 50 34 2074 34 1005 34 3678
Fragments arranged by size:
3678 2074 1005 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2473 3818 4835 6045 2473
Size: 1345 1017 1210 3371
Fragments arranged by size:
3371 1345 1210 1017
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6943
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5554 136
Size: 107 122 71 631 605 337 3545 1525
Fragments arranged by size:
3545 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6938 6938
Size: 6943
BlpI GC'TnA_GC
Cuts at: 3083 3689 4360 3083
Size: 606 671 5666
Fragments arranged by size:
5666 671 606
Bme1580I G_kGCm'C
Cuts at: 1790 2241 2520 3538 5004 6160 1790
Size: 451 279 1018 1466 1156 2573
Fragments arranged by size:
2573 1466 1156 1018 451 279
BmgBI CAC'GTC
Cuts at: 1318 2623 2860 1318
Size: 1305 237 5401
Fragments arranged by size:
5401 1305 237
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2145 3520 4674
Size: 416 53 83 399 24 728 1375 1154
Cuts at: 4674 5632 442
Size: 958 1753
Fragments arranged by size:
1753 1375 1154 958 728 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6943
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2257 2410 3068 3372 5603 1239
Size: 1018 153 658 304 2231 2579
Fragments arranged by size:
2579 2231 1018 658 304 153
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5068 5933 6231 5068
Size: 865 298 5780
Fragments arranged by size:
5780 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2487 3481 4207 5606 882
Size: 617 988 994 726 1399 2219
Fragments arranged by size:
2219 1399 994 988 726 617
BsaAI yAC'GTr
Cuts at: 493 1204 2356 3323 4734 493
Size: 711 1152 967 1411 2702
Fragments arranged by size:
2702 1411 1152 967 711
BsaBI GATnn'nnATC
Cuts at: 3829 6937 3829
Size: 3108 3835
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3681 4750 5132 5767
Size: 53 83 186 936 2148 1069 382 635
Cuts at: 5767 275
Size: 1451
Fragments arranged by size:
2148 1451 1069 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2155 5376 5774 6048 6195 6606 2155
Size: 3221 398 274 147 411 2492
Fragments arranged by size:
3221 2492 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2735 2758 2765 2788 3836
Size: 30 165 30 1332 23 7 23 1048
Cuts at: 3836 3866 3890 3920 1178
Size: 30 24 30 4201
Fragments arranged by size:
4201 1332 1048 165 30 30 30 30
24 23 23 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2113 2766 2876 1186
Size: 927 653 110 5253
Fragments arranged by size:
5253 927 653 110
BseYI C'CCAG_C
Cuts at: 2447 3486 4101 6146 6642 2447
Size: 1039 615 2045 496 2748
Fragments arranged by size:
2748 2045 1039 615 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2558 2972 1163
Size: 573 822 414 5134
Fragments arranged by size:
5134 822 573 414
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4393 5154 6182 1540
Size: 275 186 2392 761 1028 2301
Fragments arranged by size:
2392 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2241 2520 2886 3690 5004 5089 6160 2241
Size: 279 366 804 1314 85 1071 3024
Fragments arranged by size:
3024 1314 1071 804 366 279 85
BsmI GAATG_Cn'
Cuts at: 2851 4454 4547 2851
Size: 1603 93 5247
Fragments arranged by size:
5247 1603 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2241 2426 2520 2886 3538 3690 4262
Size: 399 451 185 94 366 652 152 572
Cuts at: 4262 5004 5089 6160 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 652 572 451 399 366
185 152 94 85
BspEI T'CCGG_A
Cuts at: 2155 6606 2155
Size: 4451 2492
BspHI T'CATG_A
Cuts at: 3972 4832 6562 3972
Size: 860 1730 4353
Fragments arranged by size:
4353 1730 860
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2903 3924 4298 844
Size: 2059 1021 374 3489
Fragments arranged by size:
3489 2059 1021 374
BsrBI CCG'CTC
Cuts at: 1394 1823 3817 4830 1394
Size: 429 1994 1013 3507
Fragments arranged by size:
3507 1994 1013 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3094 5438 5612 65
Size: 1183 1846 2344 174 1396
Fragments arranged by size:
2344 1846 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5587 5774 1700
Size: 110 3777 187 2869
Fragments arranged by size:
3777 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6943
BssHII G'CGCG_C
Cuts at: 1925 3785 1925
Size: 1860 5083
BssSI C'ACGA_G
Cuts at: 3015 3506 5003 6015 3015
Size: 491 1497 1012 3943
Fragments arranged by size:
3943 1497 1012 491
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2295 794
Size: 1501 5442
BstBI TT'CG_AA
Cuts at: 5747 5747
Size: 6943
BstEII G'GTnAC_C
Cuts at: 1184 3463 1184
Size: 2279 4664
BstYI r'GATC_y
Cuts at: 1972 2528 4273 4640 5025 5042 6494 6580 6592
Size: 556 1745 367 385 17 1452 86 12
Cuts at: 6592 6938 1972
Size: 346 1977
Fragments arranged by size:
1977 1745 1452 556 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2359 2359
Size: 6943
BtgI C'CryG_G
Cuts at: 513 1065 3855 513
Size: 552 2790 3601
Fragments arranged by size:
3601 2790 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2660 3162 487
Size: 683 56 1434 502 4268
Fragments arranged by size:
4268 1434 683 502 56
BtsI GCAGTG_nn'
Cuts at: 3444 3597 4459 5253 5273 3444
Size: 153 862 794 20 5114
Fragments arranged by size:
5114 862 794 153 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6943
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3218 3253 3708 3743 529
Size: 35 2654 35 455 35 3729
Fragments arranged by size:
3729 2654 455 35 35 35
DraI TTT'AAA
Cuts at: 3426 4192 4247 3426
Size: 766 55 6122
Fragments arranged by size:
6122 766 55
DraIII CAC_nnn'GTG
Cuts at: 3747 3747
Size: 6943
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5950 809
Size: 507 4634 1802
Fragments arranged by size:
4634 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2412 2638 2652
Size: 54 1425 50 275 18 582 226 14
Cuts at: 2652 3951 4390 5279 6908 8
Size: 1299 439 889 1629 43
Fragments arranged by size:
1629 1425 1299 889 582 439 275 226
54 50 43 18 14
EagI C'GGCC_G
Cuts at: 1537 1812 4390 1537
Size: 275 2578 4090
Fragments arranged by size:
4090 2578 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2804 4873 1353
Size: 1451 2069 3423
Fragments arranged by size:
3423 2069 1451
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5530 5904 6050 387
Size: 1064 138 3941 374 146 1280
Fragments arranged by size:
3941 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2257 2410 3022 3068 3132 3240 3372
Size: 393 625 153 612 46 64 108 132
Cuts at: 3372 3950 5006 5603 6390 6658 1239
Size: 578 1056 597 787 268 1524
Fragments arranged by size:
1524 1056 787 625 612 597 578 393
268 153 132 108 64 46
EcoNI CCTnn'n_nnAGG
Cuts at: 3698 3698
Size: 6943
EcoO109I rG'GnC_Cy
Cuts at: 3033 3033
Size: 6943
EcoRI G'AATT_C
Cuts at: 4252 4630 4252
Size: 378 6565
FspI TGC'GCA
Cuts at: 5449 5449
Size: 6943
HaeII r_GCGC'y
Cuts at: 1678 6090 1678
Size: 4412 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2255 2287 2486 2518 4147
Size: 32 135 32 683 32 199 32 1629
Cuts at: 4147 4179 4187 4219 5584 5616 5658 5690 1373
Size: 32 8 32 1365 32 42 32 2626
Fragments arranged by size:
2626 1629 1365 683 199 135 42 32
32 32 32 32 32 32 32 8
HincII GTy'rAC
Cuts at: 669 1657 1938 3864 4287 4533 669
Size: 988 281 1926 423 246 3079
Fragments arranged by size:
3079 1926 988 423 281 246
HindIII A'AGCT_T
Cuts at: 748 3500 748
Size: 2752 4191
HpaI GTT'AAC
Cuts at: 4533 4533
Size: 6943
KpnI G_GTAC'C
Cuts at: 2818 3781 4268 2818
Size: 963 487 5493
Fragments arranged by size:
5493 963 487
MfeI C'AATT_G
Cuts at: 2213 3947 3955 4542 2213
Size: 1734 8 587 4614
Fragments arranged by size:
4614 1734 587 8
MluI A'CGCG_T
Cuts at: 6819 6819
Size: 6943
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2707 6057 6241 1305
Size: 1402 3350 184 2007
Fragments arranged by size:
3350 2007 1402 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2654 3953 10
Size: 54 1425 343 822 1299 3000
Fragments arranged by size:
3000 1425 1299 822 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3602 3835 3971 4901 5260 5419 518
Size: 648 2436 233 136 930 359 159 2042
Fragments arranged by size:
2436 2042 930 648 359 233 159 136
MspA1I CmG'CkG
Cuts at: 1606 2042 2127 3588 4134 4153 4359 5036 6184
Size: 436 85 1461 546 19 206 677 1148
Cuts at: 6184 6429 1606
Size: 245 2120
Fragments arranged by size:
2120 1461 1148 677 546 436 245 206
85 19
NcoI C'CATG_G
Cuts at: 513 1065 3855 513
Size: 552 2790 3601
Fragments arranged by size:
3601 2790 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6943
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6943
NotI GC'GGCC_GC
Cuts at: 4390 4390
Size: 6943
NspI r_CATG'y
Cuts at: 1132 1229 2794 4150 4301 5846 1132
Size: 97 1565 1356 151 1545 2229
Fragments arranged by size:
2229 1565 1545 1356 151 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6943
PciI A'CATG_T
Cuts at: 5842 5842
Size: 6943
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6943
PfoI T'CCnGG_A
Cuts at: 2178 3045 3349 2178
Size: 867 304 5772
Fragments arranged by size:
5772 867 304
PmeI GTTT'AAAC
Cuts at: 4247 4247
Size: 6943
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3836 3868 5012 5044 6525 6557 3836
Size: 32 1144 32 1481 32 4222
Fragments arranged by size:
4222 1481 1144 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3033 3033
Size: 6943
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6943
PsiI TTA'TAA
Cuts at: 4513 4513
Size: 6943
PstI C_TGCA'G
Cuts at: 830 3451 3517 4295 830
Size: 2621 66 778 3478
Fragments arranged by size:
3478 2621 778 66
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6943
PvuII CAG'CTG
Cuts at: 2042 4134 2042
Size: 2092 4851
SalI G'TCGA_C
Cuts at: 1936 4285 1936
Size: 2349 4594
SbfI CC_TGCA'GG
Cuts at: 4295 4295
Size: 6943
ScaI AGT'ACT
Cuts at: 2671 3530 5191 6903 2671
Size: 859 1661 1712 2711
Fragments arranged by size:
2711 1712 1661 859
SexAI A'CCwGG_T
Cuts at: 4197 4197
Size: 6943
SfcI C'TryA_G
Cuts at: 826 1045 3447 3513 4291 5426 6107 6298 826
Size: 219 2402 66 778 1135 681 191 1471
Fragments arranged by size:
2402 1471 1135 778 681 219 191 66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6943
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6943
SmaI CCC'GGG
Cuts at: 1872 4270 1872
Size: 2398 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5047 5948 6210 820
Size: 197 926 3104 901 262 1553
Fragments arranged by size:
3104 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2356 4734 493
Size: 1863 2378 2702
Fragments arranged by size:
2702 2378 1863
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6943
SphI G_CATG'C
Cuts at: 1132 4301 1132
Size: 3169 3774
SspI AAT'ATT
Cuts at: 5 52 3306 3890 4867 5
Size: 47 3254 584 977 2081
Fragments arranged by size:
3254 2081 977 584 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6943
StyI C'CwwG_G
Cuts at: 513 1065 2204 3855 4382 513
Size: 552 1139 1651 527 3074
Fragments arranged by size:
3074 1651 1139 552 527
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4970 4987 5140 5325 5770 325
Size: 1255 3390 17 153 185 445 1498
Fragments arranged by size:
3390 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2669 2787 3528
Size: 274 80 33 51 1443 692 118 741
Cuts at: 3528 5189 6901 96
Size: 1661 1712 138
Fragments arranged by size:
1712 1661 1443 741 692 274 138 118
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2758 2790 6903 6935 2758
Size: 32 4113 32 2766
Fragments arranged by size:
4113 2766 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4181 4199 1316
Size: 2865 18 4060
Fragments arranged by size:
4060 2865 18
XbaI T'CTAG_A
Cuts at: 4279 4279
Size: 6943
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3004 3004
Size: 6943
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6943
XmaI C'CCGG_G
Cuts at: 1870 4268 1870
Size: 2398 4545
XmnI GAAnn'nnTTC
Cuts at: 5072 5072
Size: 6943
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4751 276
Size: 53 83 186 936 3217 2468
Fragments arranged by size:
3217 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI Bsu36I EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI