Restriction map for
pFN21AB5747
(Circular) (Six-base) MAPSORT of: pFN21AB5747.seq Check: 6532 from: 1 to: 7126
pFN21AB5747 7126 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4936 278
Size: 53 83 186 936 3400 2468
Fragments arranged by size:
3400 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2850 4469 1937
Size: 913 1619 4594
Fragments arranged by size:
4594 1619 913
Acc65I G'GTAC_C
Cuts at: 3635 4447 3635
Size: 812 6314
AclI AA'CG_TT
Cuts at: 1563 5253 5626 1563
Size: 3690 373 3063
Fragments arranged by size:
3690 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2111 2755 3215 3468 4241 5189 6573 6841
Size: 479 644 460 253 773 948 1384 268
Cuts at: 6841 1632
Size: 1917
Fragments arranged by size:
1917 1384 948 773 644 479 460 268
253
AfeI AGC'GCT
Cuts at: 2426 2426
Size: 7126
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6929
AflIII A'CryG_T
Cuts at: 1203 6025 7002 1203
Size: 4822 977 1327
Fragments arranged by size:
4822 1327 977
AgeI A'CCGG_T
Cuts at: 5957 5957
Size: 7126
AhdI GACnn_n'nnGTC
Cuts at: 5855 5855
Size: 7126
AleI CACnn'nnGTG
Cuts at: 1166 2685 1166
Size: 1519 5607
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7126
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3673 3705 3673
Size: 32 7094
AlwNI CAG_nnn'CTG
Cuts at: 4219 4325 4869 6441 4219
Size: 106 544 1572 4904
Fragments arranged by size:
4904 1572 544 106
ApaLI G'TGCA_C
Cuts at: 5183 6339 5183
Size: 1156 5970
ApoI r'AATT_y
Cuts at: 1493 2019 2084 2459 3763 3902 3995 4195 4263
Size: 526 65 375 1304 139 93 200 68
Cuts at: 4263 4357 4435 4661 4813 6922 1493
Size: 94 78 226 152 2109 1697
Fragments arranged by size:
2109 1697 1304 526 375 226 200 152
139 94 93 78 68 65
AseI AT'TA_AT
Cuts at: 160 1032 5680 160
Size: 872 4648 1606
Fragments arranged by size:
4648 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7126
AvaI C'yCGr_G
Cuts at: 1870 1943 4451 1870
Size: 73 2508 4545
Fragments arranged by size:
4545 2508 73
BamHI G'GATC_C
Cuts at: 4456 4823 4456
Size: 367 6759
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3328 3635 4447 5902 618
Size: 325 217 627 1541 307 812 1455 1842
Fragments arranged by size:
1842 1541 1455 812 627 325 307 217
BanII G_rGCy'C
Cuts at: 1391 2628 3260 4445 1391
Size: 1237 632 1185 4072
Fragments arranged by size:
4072 1237 1185 632
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4229 928
Size: 971 2330 3825
Fragments arranged by size:
3825 2330 971
BbvCI CC'TCA_GC
Cuts at: 3934 3934
Size: 7126
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5317 5351 1903
Size: 34 3380 34 3678
Fragments arranged by size:
3678 3380 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2488 5018 6228 2488
Size: 2530 1210 3386
Fragments arranged by size:
3386 2530 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7126
BfrBI ATG'CAT
Cuts at: 2391 2676 3475 2391
Size: 285 799 6042
Fragments arranged by size:
6042 799 285
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5737 136
Size: 107 122 71 631 605 4065 1525
Fragments arranged by size:
4065 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7121 7121
Size: 7126
BlpI GC'TnA_GC
Cuts at: 2436 4543 2436
Size: 2107 5019
Bme1580I G_kGCm'C
Cuts at: 1790 5187 6343 1790
Size: 3397 1156 2573
Fragments arranged by size:
3397 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7126
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2440 4857 5815
Size: 416 53 83 399 24 1023 2417 958
Cuts at: 5815 442
Size: 1753
Fragments arranged by size:
2417 1753 1023 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2365 3167 3918 4327 5786 1239
Size: 1126 802 751 409 1459 2579
Fragments arranged by size:
2579 1459 1126 802 751 409
Bpu10I CC'TnA_GC
Cuts at: 3934 3934
Size: 7126
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2642 5251 6116 6414 2642
Size: 2609 865 298 3354
Fragments arranged by size:
3354 2609 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5789 882
Size: 617 4290 2219
Fragments arranged by size:
4290 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3533 4917 493
Size: 711 2329 1384 2702
Fragments arranged by size:
2702 2329 1384 711
BsaBI GATnn'nnATC
Cuts at: 7120 7120
Size: 7126
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4933 5315 5950 275
Size: 53 83 186 936 3400 382 635 1451
Fragments arranged by size:
3400 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5559 5957 6231 6378 6789 5559
Size: 398 274 147 411 5896
Fragments arranged by size:
5896 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4077 4107 4240 4270 1178
Size: 30 165 30 2674 30 133 30 4034
Fragments arranged by size:
4034 2674 165 133 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2084 4080 1186
Size: 898 1996 4232
Fragments arranged by size:
4232 1996 898
BseYI C'CCAG_C
Cuts at: 6329 6825 6329
Size: 496 6630
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6553
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4576 5337 6365 1540
Size: 275 186 2575 761 1028 2301
Fragments arranged by size:
2575 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2628 3571 5187 5272 6343 2628
Size: 943 1616 85 1071 3411
Fragments arranged by size:
3411 1616 1071 943 85
BsmI GAATG_Cn'
Cuts at: 2676 4637 4730 2676
Size: 1961 93 5072
Fragments arranged by size:
5072 1961 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2628 3260 3571 4445 5187 5272 6343
Size: 399 838 632 311 874 742 85 1071
Cuts at: 6343 1391
Size: 2174
Fragments arranged by size:
2174 1071 874 838 742 632 399 311
85
BspEI T'CCGG_A
Cuts at: 6789 6789
Size: 7126
BspHI T'CATG_A
Cuts at: 5015 6745 5015
Size: 1730 5396
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4481 844
Size: 3637 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5013 1394
Size: 429 3190 3507
Fragments arranged by size:
3507 3190 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5621 5795 65
Size: 1183 4373 174 1396
Fragments arranged by size:
4373 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5770 5957 1700
Size: 110 3960 187 2869
Fragments arranged by size:
3960 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7126
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7126
BssSI C'ACGA_G
Cuts at: 5186 6198 5186
Size: 1012 6114
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2682 794
Size: 1888 5238
BstBI TT'CG_AA
Cuts at: 5930 5930
Size: 7126
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7126
BstXI CCAn_nnnn'nTGG
Cuts at: 3391 4416 3391
Size: 1025 6101
BstYI r'GATC_y
Cuts at: 1972 4456 4823 5208 5225 6677 6763 6775 7121
Size: 2484 367 385 17 1452 86 12 346
Cuts at: 7121 1972
Size: 1977
Fragments arranged by size:
2484 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 4188 513
Size: 552 939 2184 3451
Fragments arranged by size:
3451 2184 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6387
Fragments arranged by size:
6387 683 56
BtsI GCAGTG_nn'
Cuts at: 2680 2692 3503 4642 5436 5456 2680
Size: 12 811 1139 794 20 4350
Fragments arranged by size:
4350 1139 811 794 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7126
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7091
DraI TTT'AAA
Cuts at: 4430 4430
Size: 7126
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6133 809
Size: 507 4817 1802
Fragments arranged by size:
4817 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2582 4573 5462
Size: 54 1425 50 275 18 752 1991 889
Cuts at: 5462 7091 8
Size: 1629 43
Fragments arranged by size:
1991 1629 1425 889 752 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4573 1537
Size: 275 2761 4090
Fragments arranged by size:
4090 2761 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3093 3234 3731 5056 1353
Size: 1740 141 497 1325 3423
Fragments arranged by size:
3423 1740 1325 497 141
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5713 6087 6233 387
Size: 1064 138 4124 374 146 1280
Fragments arranged by size:
4124 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2626 2626
Size: 7126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2365 2755 3167 3215 3468 3918
Size: 393 479 254 390 412 48 253 450
Cuts at: 3918 4241 4327 5189 5786 6573 6841 1239
Size: 323 86 862 597 787 268 1524
Fragments arranged by size:
1524 862 787 597 479 450 412 393
390 323 268 254 253 86 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2039 2039
Size: 7126
EcoO109I rG'GnC_Cy
Cuts at: 2315 2315
Size: 7126
EcoRI G'AATT_C
Cuts at: 3763 3902 4435 4813 3763
Size: 139 533 378 6076
Fragments arranged by size:
6076 533 378 139
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3150 3424 3150
Size: 274 6852
FspI TGC'GCA
Cuts at: 5632 5632
Size: 7126
HaeII r_GCGC'y
Cuts at: 1678 2428 6273 1678
Size: 750 3845 2531
Fragments arranged by size:
3845 2531 750
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3242 3274 3445 3477 3740
Size: 32 135 32 1670 32 171 32 263
Cuts at: 3740 3772 5767 5799 5841 5873 1373
Size: 32 1995 32 42 32 2626
Fragments arranged by size:
2626 1995 1670 263 171 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4470 4716 669
Size: 988 281 2532 246 3079
Fragments arranged by size:
3079 2532 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7126
HpaI GTT'AAC
Cuts at: 4716 4716
Size: 7126
KpnI G_GTAC'C
Cuts at: 3639 4451 3639
Size: 812 6314
MfeI C'AATT_G
Cuts at: 4725 4725
Size: 7126
MluI A'CGCG_T
Cuts at: 7002 7002
Size: 7126
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3794 6240 6424 1305
Size: 2489 2446 184 2007
Fragments arranged by size:
2489 2446 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2584 10
Size: 54 1425 343 752 4552
Fragments arranged by size:
4552 1425 752 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2381 2685 3185 3967 5084 5443 5602
Size: 648 1215 304 500 782 1117 359 159
Cuts at: 5602 518
Size: 2042
Fragments arranged by size:
2042 1215 1117 782 648 500 359 304
159
MspA1I CmG'CkG
Cuts at: 1606 2455 3806 3938 4542 5219 6367 6612 1606
Size: 849 1351 132 604 677 1148 245 2120
Fragments arranged by size:
2120 1351 1148 849 677 604 245 132
NcoI C'CATG_G
Cuts at: 513 1065 2004 4188 513
Size: 552 939 2184 3451
Fragments arranged by size:
3451 2184 939 552
NdeI CA'TA_TG
Cuts at: 387 2672 3511 387
Size: 2285 839 4002
Fragments arranged by size:
4002 2285 839
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7126
NotI GC'GGCC_GC
Cuts at: 4573 4573
Size: 7126
NsiI A_TGCA'T
Cuts at: 2393 2678 3477 2393
Size: 285 799 6042
Fragments arranged by size:
6042 799 285
NspI r_CATG'y
Cuts at: 1132 1229 2391 3479 4484 6029 1132
Size: 97 1162 1088 1005 1545 2229
Fragments arranged by size:
2229 1545 1162 1088 1005 97
PasI CC'CwG_GG
Cuts at: 1348 2311 1348
Size: 963 6163
PciI A'CATG_T
Cuts at: 6025 6025
Size: 7126
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3192 3218 1108
Size: 2084 26 5016
Fragments arranged by size:
5016 2084 26
PfoI T'CCnGG_A
Cuts at: 2034 2334 2790 4228 2034
Size: 300 456 1438 4932
Fragments arranged by size:
4932 1438 456 300
PmeI GTTT'AAAC
Cuts at: 4430 4430
Size: 7126
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5195 5227 6708 6740 5195
Size: 32 1481 32 5581
Fragments arranged by size:
5581 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2315 2315
Size: 7126
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7126
PsiI TTA'TAA
Cuts at: 4696 4696
Size: 7126
PstI C_TGCA'G
Cuts at: 830 2028 2687 4321 4478 830
Size: 1198 659 1634 157 3478
Fragments arranged by size:
3478 1634 1198 659 157
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7126
PvuII CAG'CTG
Cuts at: 2455 3806 3938 2455
Size: 1351 132 5643
Fragments arranged by size:
5643 1351 132
SacI G_AGCT'C
Cuts at: 2628 2628
Size: 7126
SalI G'TCGA_C
Cuts at: 1936 4468 1936
Size: 2532 4594
SapI GCTCTTCn'nnn_
Cuts at: 3093 3093
Size: 7126
SbfI CC_TGCA'GG
Cuts at: 4478 4478
Size: 7126
ScaI AGT'ACT
Cuts at: 5374 7086 5374
Size: 1712 5414
SfcI C'TryA_G
Cuts at: 826 1045 2024 2607 2683 4155 4317 4474 5609
Size: 219 979 583 76 1472 162 157 1135
Cuts at: 5609 6290 6481 826
Size: 681 191 1471
Fragments arranged by size:
1472 1471 1135 979 681 583 219 191
162 157 76
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7126
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7126
SmaI CCC'GGG
Cuts at: 1872 4453 1872
Size: 2581 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2621 5230 6131 6393 820
Size: 197 926 678 2609 901 262 1553
Fragments arranged by size:
2609 1553 926 901 678 262 197
SnaBI TAC'GTA
Cuts at: 493 3533 4917 493
Size: 3040 1384 2702
Fragments arranged by size:
3040 2702 1384
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7126
SphI G_CATG'C
Cuts at: 1132 2391 4484 1132
Size: 1259 2093 3774
Fragments arranged by size:
3774 2093 1259
SspI AAT'ATT
Cuts at: 5 52 3778 4423 5050 5
Size: 47 3726 645 627 2081
Fragments arranged by size:
3726 2081 645 627 47
StuI AGG'CCT
Cuts at: 1517 2812 1517
Size: 1295 5831
StyI C'CwwG_G
Cuts at: 513 1065 2004 2292 3384 3824 4188 4397 4565
Size: 552 939 288 1092 440 364 209 168
Cuts at: 4565 513
Size: 3074
Fragments arranged by size:
3074 1092 939 552 440 364 288 209
168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2324 5153 5170 5323 5508 5953 325
Size: 1255 744 2829 17 153 185 445 1498
Fragments arranged by size:
2829 1498 1255 744 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2115 3808 5372
Size: 274 80 33 51 1443 138 1693 1564
Cuts at: 5372 7084 96
Size: 1712 138
Fragments arranged by size:
1712 1693 1564 1443 274 138 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2299 2331 4077 4109 7086 7118 2299
Size: 32 1746 32 2977 32 2307
Fragments arranged by size:
2977 2307 1746 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7126
XbaI T'CTAG_A
Cuts at: 4108 4462 4108
Size: 354 6772
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4413 4413
Size: 7126
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7126
XmaI C'CCGG_G
Cuts at: 1870 4451 1870
Size: 2581 4545
XmnI GAAnn'nnTTC
Cuts at: 4326 5255 4326
Size: 929 6197
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4934 276
Size: 53 83 186 936 3400 2468
Fragments arranged by size:
3400 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI