Restriction map for
pFN21AB5742
(Circular) (Six-base) MAPSORT of: pFN21AB5742.seq Check: 15 from: 1 to: 6928
pFN21AB5742 6928 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4738 278
Size: 53 83 186 936 3202 2468
Fragments arranged by size:
3202 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4113 4271 1937
Size: 2176 158 4594
Fragments arranged by size:
4594 2176 158
Acc65I G'GTAC_C
Cuts at: 4249 4249
Size: 6928
AclI AA'CG_TT
Cuts at: 1563 5055 5428 1563
Size: 3492 373 3063
Fragments arranged by size:
3492 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2879 3024 3065 3111 4991 6375 6643 1632
Size: 1247 145 41 46 1880 1384 268 1917
Fragments arranged by size:
1917 1880 1384 1247 268 145 46 41
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6731
AflIII A'CryG_T
Cuts at: 1203 2542 3718 5827 6804 1203
Size: 1339 1176 2109 977 1327
Fragments arranged by size:
2109 1339 1327 1176 977
AgeI A'CCGG_T
Cuts at: 5759 5759
Size: 6928
AhdI GACnn_n'nnGTC
Cuts at: 3584 5657 3584
Size: 2073 4855
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6928
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6928
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2223 2255 2223
Size: 32 6896
AlwNI CAG_nnn'CTG
Cuts at: 3325 3460 4671 6243 3325
Size: 135 1211 1572 4010
Fragments arranged by size:
4010 1572 1211 135
ApaLI G'TGCA_C
Cuts at: 3204 3344 4985 6141 3204
Size: 140 1641 1156 3991
Fragments arranged by size:
3991 1641 1156 140
ApoI r'AATT_y
Cuts at: 1493 2429 2458 3380 4006 4237 4463 4615 6724
Size: 936 29 922 626 231 226 152 2109
Cuts at: 6724 1493
Size: 1697
Fragments arranged by size:
2109 1697 936 922 626 231 226 152
29
AseI AT'TA_AT
Cuts at: 160 1032 2802 3912 5482 160
Size: 872 1770 1110 1570 1606
Fragments arranged by size:
1770 1606 1570 1110 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6928
AvaI C'yCGr_G
Cuts at: 1870 1943 4253 1870
Size: 73 2310 4545
Fragments arranged by size:
4545 2310 73
BamHI G'GATC_C
Cuts at: 4258 4625 4258
Size: 367 6561
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4249 5704 618
Size: 325 217 627 2462 1455 1842
Fragments arranged by size:
2462 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2642 3026 4247 1391
Size: 1251 384 1221 4072
Fragments arranged by size:
4072 1251 1221 384
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2082 2729 3012 3185 4091 928
Size: 971 183 647 283 173 906 3765
Fragments arranged by size:
3765 971 906 647 283 183 173
BbvCI CC'TCA_GC
Cuts at: 2495 2495
Size: 6928
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5119 5153 1903
Size: 34 3182 34 3678
Fragments arranged by size:
3678 3182 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3437 4820 6030 3437
Size: 1383 1210 4335
Fragments arranged by size:
4335 1383 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6928
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3285 5539 136
Size: 107 122 71 631 605 1613 2254 1525
Fragments arranged by size:
2254 1613 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3562 4096 6923 3562
Size: 534 2827 3567
Fragments arranged by size:
3567 2827 534
BlpI GC'TnA_GC
Cuts at: 4345 4345
Size: 6928
Bme1580I G_kGCm'C
Cuts at: 1790 3208 3348 4989 6145 1790
Size: 1418 140 1641 1156 2573
Fragments arranged by size:
2573 1641 1418 1156 140
BmgBI CAC'GTC
Cuts at: 1318 2046 1318
Size: 728 6200
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3040 4659 5617
Size: 416 53 83 399 24 1623 1619 958
Cuts at: 5617 442
Size: 1753
Fragments arranged by size:
1753 1623 1619 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6928
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2035 2801 3558 3651 5588 1239
Size: 796 766 757 93 1937 2579
Fragments arranged by size:
2579 1937 796 766 757 93
Bpu10I CC'TnA_GC
Cuts at: 2495 2636 2495
Size: 141 6787
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3071 5053 5918 6216 3071
Size: 1982 865 298 3783
Fragments arranged by size:
3783 1982 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4056 5591 882
Size: 617 2557 1535 2219
Fragments arranged by size:
2557 2219 1535 617
BsaAI yAC'GTr
Cuts at: 493 1204 2545 3203 4719 493
Size: 711 1341 658 1516 2702
Fragments arranged by size:
2702 1516 1341 711 658
BsaBI GATnn'nnATC
Cuts at: 6922 6922
Size: 6928
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4735 5117 5752 275
Size: 53 83 186 936 3202 382 635 1451
Fragments arranged by size:
3202 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4002 5361 5759 6033 6180 6591 4002
Size: 1359 398 274 147 411 4339
Fragments arranged by size:
4339 1359 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2139 2169 2223 2253 2340
Size: 30 165 30 736 30 54 30 87
Cuts at: 2340 2370 2439 2469 1178
Size: 30 69 30 5637
Fragments arranged by size:
5637 736 165 87 69 54 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2460 1186
Size: 1274 5654
BseYI C'CCAG_C
Cuts at: 3745 6131 6627 3745
Size: 2386 496 4046
Fragments arranged by size:
4046 2386 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2789 1163
Size: 573 1053 5302
Fragments arranged by size:
5302 1053 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4378 5139 6167 1540
Size: 275 186 2377 761 1028 2301
Fragments arranged by size:
2377 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2642 2793 3026 3208 3348 4989 5074 6145 2642
Size: 151 233 182 140 1641 85 1071 3425
Fragments arranged by size:
3425 1641 1071 233 182 151 140 85
BsmI GAATG_Cn'
Cuts at: 4439 4532 4439
Size: 93 6835
BsmBI CGTCTCn'nnnn_
Cuts at: 3704 3704
Size: 6928
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2642 2793 3026 3208 3348 4247 4989
Size: 399 852 151 233 182 140 899 742
Cuts at: 4989 5074 6145 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 899 852 742 399 233 182
151 140 85
BspEI T'CCGG_A
Cuts at: 4002 6591 4002
Size: 2589 4339
BspHI T'CATG_A
Cuts at: 4817 6547 4817
Size: 1730 5198
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3750 4283 844
Size: 2906 533 3489
Fragments arranged by size:
3489 2906 533
BsrBI CCG'CTC
Cuts at: 1394 1823 2491 4815 1394
Size: 429 668 2324 3507
Fragments arranged by size:
3507 2324 668 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5423 5597 65
Size: 1183 4175 174 1396
Fragments arranged by size:
4175 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5572 5759 1700
Size: 110 3762 187 2869
Fragments arranged by size:
3762 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6928
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6928
BssSI C'ACGA_G
Cuts at: 4988 6000 4988
Size: 1012 5916
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2306 2677 794
Size: 1512 371 5045
Fragments arranged by size:
5045 1512 371
BstBI TT'CG_AA
Cuts at: 5732 5732
Size: 6928
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6928
BstXI CCAn_nnnn'nTGG
Cuts at: 4210 4210
Size: 6928
BstYI r'GATC_y
Cuts at: 1972 2911 3049 3457 3541 3562 3625 3843 4096
Size: 939 138 408 84 21 63 218 253
Cuts at: 4096 4258 4625 5010 5027 6479 6565 6577 6923
Size: 162 367 385 17 1452 86 12 346
Cuts at: 6923 1972
Size: 1977
Fragments arranged by size:
1977 1452 939 408 385 367 346 253
218 162 138 86 84 63 21 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2686 513
Size: 552 939 682 4755
Fragments arranged by size:
4755 939 682 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6189
Fragments arranged by size:
6189 683 56
BtsI GCAGTG_nn'
Cuts at: 2076 2748 2831 4444 5238 5258 2076
Size: 672 83 1613 794 20 3746
Fragments arranged by size:
3746 1613 794 672 83 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6928
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6893
DraI TTT'AAA
Cuts at: 4232 4232
Size: 6928
DraIII CAC_nnn'GTG
Cuts at: 3256 3256
Size: 6928
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5935 809
Size: 507 4619 1802
Fragments arranged by size:
4619 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4212 4375 5264
Size: 54 1425 50 275 18 2382 163 889
Cuts at: 5264 6893 8
Size: 1629 43
Fragments arranged by size:
2382 1629 1425 889 275 163 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4375 1537
Size: 275 2563 4090
Fragments arranged by size:
4090 2563 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2163 3642 3707 4858 1353
Size: 810 1479 65 1151 3423
Fragments arranged by size:
3423 1479 1151 810 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2947 5515 5889 6035 387
Size: 1064 138 1358 2568 374 146 1280
Fragments arranged by size:
2568 1358 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2640 3024 2640
Size: 384 6544
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2035 2801 2879 3024 3065 3111 3558
Size: 393 403 766 78 145 41 46 447
Cuts at: 3558 3651 4991 5588 6375 6643 1239
Size: 93 1340 597 787 268 1524
Fragments arranged by size:
1524 1340 787 766 597 447 403 393
268 145 93 78 46 41
EcoNI CCTnn'n_nnAGG
Cuts at: 2954 2954
Size: 6928
EcoO109I rG'GnC_Cy
Cuts at: 2039 3571 3685 2039
Size: 1532 114 5282
Fragments arranged by size:
5282 1532 114
EcoRI G'AATT_C
Cuts at: 4237 4615 4237
Size: 378 6550
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2773 2773
Size: 6928
FspI TGC'GCA
Cuts at: 5434 5434
Size: 6928
HaeII r_GCGC'y
Cuts at: 1678 6075 1678
Size: 4397 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2002 2034 2340 2372 2614
Size: 32 135 32 430 32 306 32 242
Cuts at: 2614 2646 3570 3602 3715 3747 5569 5601 5643
Size: 32 924 32 113 32 1822 32 42
Cuts at: 5643 5675 1373
Size: 32 2626
Fragments arranged by size:
2626 1822 924 430 306 242 135 113
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2119 4272 4518 669
Size: 988 281 181 2153 246 3079
Fragments arranged by size:
3079 2153 988 281 246 181
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6928
HpaI GTT'AAC
Cuts at: 4518 4518
Size: 6928
KpnI G_GTAC'C
Cuts at: 4253 4253
Size: 6928
MfeI C'AATT_G
Cuts at: 2132 4527 2132
Size: 2395 4533
MluI A'CGCG_T
Cuts at: 6804 6804
Size: 6928
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3837 6042 6226 1305
Size: 2532 2205 184 2007
Fragments arranged by size:
2532 2205 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4214 10
Size: 54 1425 343 2382 2724
Fragments arranged by size:
2724 2382 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4023 4886 5245 5404 518
Size: 648 2857 863 359 159 2042
Fragments arranged by size:
2857 2042 863 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2055 2402 3211 4344 5021 6169 6414 1606
Size: 449 347 809 1133 677 1148 245 2120
Fragments arranged by size:
2120 1148 1133 809 677 449 347 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2686 513
Size: 552 939 682 4755
Fragments arranged by size:
4755 939 682 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6928
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6928
NotI GC'GGCC_GC
Cuts at: 4375 4375
Size: 6928
NspI r_CATG'y
Cuts at: 1132 1229 3722 4286 5831 1132
Size: 97 2493 564 1545 2229
Fragments arranged by size:
2493 2229 1545 564 97
PasI CC'CwG_GG
Cuts at: 1348 4220 1348
Size: 2872 4056
PciI A'CATG_T
Cuts at: 3718 5827 3718
Size: 2109 4819
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6928
PfoI T'CCnGG_A
Cuts at: 2163 2163
Size: 6928
PmeI GTTT'AAAC
Cuts at: 4232 4232
Size: 6928
PmlI CAC'GTG
Cuts at: 3203 3203
Size: 6928
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2223 2255 4997 5029 6510 6542 2223
Size: 32 2742 32 1481 32 2609
Fragments arranged by size:
2742 2609 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2039 3571 3685 2039
Size: 1532 114 5282
Fragments arranged by size:
5282 1532 114
PshAI GACnn'nnGTC
Cuts at: 1613 2044 1613
Size: 431 6497
PsiI TTA'TAA
Cuts at: 2439 4498 2439
Size: 2059 4869
PstI C_TGCA'G
Cuts at: 830 2071 4280 830
Size: 1241 2209 3478
Fragments arranged by size:
3478 2209 1241
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6928
SacI G_AGCT'C
Cuts at: 2642 3026 2642
Size: 384 6544
SalI G'TCGA_C
Cuts at: 1936 4270 1936
Size: 2334 4594
SanDI GG'GwC_CC
Cuts at: 2039 2039
Size: 6928
SbfI CC_TGCA'GG
Cuts at: 4280 4280
Size: 6928
ScaI AGT'ACT
Cuts at: 5176 6888 5176
Size: 1712 5216
SexAI A'CCwGG_T
Cuts at: 2121 2121
Size: 6928
SfcI C'TryA_G
Cuts at: 826 1045 2067 3781 4124 4276 5411 6092 6283
Size: 219 1022 1714 343 152 1135 681 191
Cuts at: 6283 826
Size: 1471
Fragments arranged by size:
1714 1471 1135 1022 681 343 219 191
152
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6928
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6928
SmaI CCC'GGG
Cuts at: 1872 4255 1872
Size: 2383 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3086 5032 5933 6195 820
Size: 197 926 1143 1946 901 262 1553
Fragments arranged by size:
1946 1553 1143 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4719 493
Size: 4226 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6928
SphI G_CATG'C
Cuts at: 1132 4286 1132
Size: 3154 3774
SspI AAT'ATT
Cuts at: 5 52 4852 5
Size: 47 4800 2081
Fragments arranged by size:
4800 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6928
StyI C'CwwG_G
Cuts at: 513 1065 2004 2686 3574 4367 513
Size: 552 939 682 888 793 3074
Fragments arranged by size:
3074 939 888 793 682 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3464 3971 4212 4955 4972 5125 5310
Size: 1255 1884 507 241 743 17 153 185
Cuts at: 5310 5755 325
Size: 445 1498
Fragments arranged by size:
1884 1498 1255 743 507 445 241 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2203 2897 5174
Size: 274 80 33 51 1443 226 694 2277
Cuts at: 5174 6886 96
Size: 1712 138
Fragments arranged by size:
2277 1712 1443 694 274 226 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2439 2471 6888 6920 2439
Size: 32 4417 32 2447
Fragments arranged by size:
4417 2447 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2123 1316
Size: 807 6121
XbaI T'CTAG_A
Cuts at: 3565 4264 3565
Size: 699 6229
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2518 2518
Size: 6928
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6928
XmaI C'CCGG_G
Cuts at: 1870 4253 1870
Size: 2383 4545
XmnI GAAnn'nnTTC
Cuts at: 2333 2988 3182 5057 2333
Size: 655 194 1875 4204
Fragments arranged by size:
4204 1875 655 194
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4736 276
Size: 53 83 186 936 3202 2468
Fragments arranged by size:
3202 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI
PsrI PvuII RsrII SacII SapI SfiI SfoI SrfI
SwaI