Restriction map for
pFN21AB5740
(Circular) (Six-base) MAPSORT of: pFN21AB5740.seq Check: 4087 from: 1 to: 6925
pFN21AB5740 6925 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2299 3675 2299
Size: 1376 5549
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3639 4735 278
Size: 53 83 186 936 2103 1096 2468
Fragments arranged by size:
2468 2103 1096 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4268 1937
Size: 2331 4594
Acc65I G'GTAC_C
Cuts at: 3161 3831 4246 3161
Size: 670 415 5840
Fragments arranged by size:
5840 670 415
AclI AA'CG_TT
Cuts at: 1563 5052 5425 1563
Size: 3489 373 3063
Fragments arranged by size:
3489 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4988 6372 6640 1632
Size: 3356 1384 268 1917
Fragments arranged by size:
3356 1917 1384 268
AfeI AGC'GCT
Cuts at: 4151 4151
Size: 6925
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6728
AflIII A'CryG_T
Cuts at: 1203 3783 5824 6801 1203
Size: 2580 2041 977 1327
Fragments arranged by size:
2580 2041 1327 977
AgeI A'CCGG_T
Cuts at: 5756 5756
Size: 6925
AhdI GACnn_n'nnGTC
Cuts at: 5654 5654
Size: 6925
AleI CACnn'nnGTG
Cuts at: 1166 3804 4100 1166
Size: 2638 296 3991
Fragments arranged by size:
3991 2638 296
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3291 3683 4118 4135 1184
Size: 2107 392 435 17 3974
Fragments arranged by size:
3974 2107 435 392 17
AlwNI CAG_nnn'CTG
Cuts at: 2314 2776 4138 4668 6240 2314
Size: 462 1362 530 1572 2999
Fragments arranged by size:
2999 1572 1362 530 462
ApaI G_GGCC'C
Cuts at: 2108 2357 2433 3458 2108
Size: 249 76 1025 5575
Fragments arranged by size:
5575 1025 249 76
ApaLI G'TGCA_C
Cuts at: 2422 4982 6138 2422
Size: 2560 1156 3209
Fragments arranged by size:
3209 2560 1156
ApoI r'AATT_y
Cuts at: 1493 4234 4460 4612 6721 1493
Size: 2741 226 152 2109 1697
Fragments arranged by size:
2741 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2604 2604
Size: 6925
AseI AT'TA_AT
Cuts at: 160 1032 5479 160
Size: 872 4447 1606
Fragments arranged by size:
4447 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6925
AvaI C'yCGr_G
Cuts at: 1870 1943 2356 2493 3113 3392 3538 4250 1870
Size: 73 413 137 620 279 146 712 4545
Fragments arranged by size:
4545 712 620 413 279 146 137 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3153 3186 3153
Size: 33 6892
BamHI G'GATC_C
Cuts at: 3035 4255 4622 3035
Size: 1220 367 5338
Fragments arranged by size:
5338 1220 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3161 3705 3711 3831 3935
Size: 325 217 627 1374 544 6 120 104
Cuts at: 3935 4246 5701 618
Size: 311 1455 1842
Fragments arranged by size:
1842 1455 1374 627 544 325 311 217
120 104 6
BanII G_rGCy'C
Cuts at: 1391 2108 2289 2357 2433 2563 3245 3458 4013
Size: 717 181 68 76 130 682 213 555
Cuts at: 4013 4212 4244 1391
Size: 199 32 4072
Fragments arranged by size:
4072 717 682 555 213 199 181 130
76 68 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3738 3987 928
Size: 971 1839 249 3866
Fragments arranged by size:
3866 1839 971 249
BbvCI CC'TCA_GC
Cuts at: 2390 3495 3722 2390
Size: 1105 227 5593
Fragments arranged by size:
5593 1105 227
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3094 3128 5116 5150 1903
Size: 34 50 34 1073 34 1988 34 3678
Fragments arranged by size:
3678 1988 1073 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4817 6027 4817
Size: 1210 5715
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6925
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2214 2371 2582
Size: 107 122 71 631 605 542 157 211
Cuts at: 2582 3971 5536 136
Size: 1389 1565 1525
Fragments arranged by size:
1565 1525 1389 631 605 542 211 157
122 107 71
BglII A'GATC_T
Cuts at: 6920 6920
Size: 6925
BlpI GC'TnA_GC
Cuts at: 2438 4342 2438
Size: 1904 5021
Bme1580I G_kGCm'C
Cuts at: 1790 2108 2357 2426 2433 3458 3716 3811 3938
Size: 318 249 69 7 1025 258 95 127
Cuts at: 3938 4986 6142 1790
Size: 1048 1156 2573
Fragments arranged by size:
2573 1156 1048 1025 318 258 249 127
95 69 7
BmgBI CAC'GTC
Cuts at: 1318 3786 1318
Size: 2468 4457
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4656 5614 442
Size: 416 53 83 399 24 3239 958 1753
Fragments arranged by size:
3239 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6925
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2222 2318 2613 2756 3316 4024 4196 5585
Size: 983 96 295 143 560 708 172 1389
Cuts at: 5585 1239
Size: 2579
Fragments arranged by size:
2579 1389 983 708 560 295 172 143
96
Bpu10I CC'TnA_GC
Cuts at: 2273 2390 2996 3495 3722 2273
Size: 117 606 499 227 5476
Fragments arranged by size:
5476 606 499 227 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2893 3425 4000 5050 5915 6213 2893
Size: 532 575 1050 865 298 3605
Fragments arranged by size:
3605 1050 865 575 532 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2673 3201 3767 5588 882
Size: 617 1174 528 566 1821 2219
Fragments arranged by size:
2219 1821 1174 617 566 528
BsaAI yAC'GTr
Cuts at: 493 1204 4716 493
Size: 711 3512 2702
Fragments arranged by size:
3512 2702 711
BsaBI GATnn'nnATC
Cuts at: 3651 6919 3651
Size: 3268 3657
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2247 3636 4732 5114
Size: 53 83 186 936 714 1389 1096 382
Cuts at: 5114 5749 275
Size: 635 1451
Fragments arranged by size:
1451 1389 1096 936 714 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5358 5756 6030 6177 6588 5358
Size: 398 274 147 411 5695
Fragments arranged by size:
5695 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3314 3344 1178
Size: 30 165 30 1911 30 4759
Fragments arranged by size:
4759 1911 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2516 2960 3393 3605 3608 3626 4021 1186
Size: 1330 444 433 212 3 18 395 4090
Fragments arranged by size:
4090 1330 444 433 395 212 18 3
BseYI C'CCAG_C
Cuts at: 2048 2052 2349 2548 2794 3072 3176 3369 3858
Size: 4 297 199 246 278 104 193 489
Cuts at: 3858 4037 4138 4204 6128 6624 2048
Size: 179 101 66 1924 496 2349
Fragments arranged by size:
2349 1924 496 489 297 278 246 199
193 179 104 101 66 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2360 2524 1163
Size: 573 624 164 5564
Fragments arranged by size:
5564 624 573 164
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4375 5136 6164 1540
Size: 275 186 2374 761 1028 2301
Fragments arranged by size:
2374 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2279 2289 2426 4986 5071 6142 2279
Size: 10 137 2560 85 1071 3062
Fragments arranged by size:
3062 2560 1071 137 85 10
BsmI GAATG_Cn'
Cuts at: 4436 4529 4436
Size: 93 6832
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2108 2279 2289 2357 2426 2433 2563
Size: 399 318 171 10 68 69 7 130
Cuts at: 2563 3245 3458 3716 3811 3938 4013 4212 4244
Size: 682 213 258 95 127 75 199 32
Cuts at: 4244 4986 5071 6142 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 682 399 318 258 213
199 171 130 127 95 85 75 69
68 32 10 7
BspEI T'CCGG_A
Cuts at: 6588 6588
Size: 6925
BspHI T'CATG_A
Cuts at: 4814 6544 4814
Size: 1730 5195
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2268 2299 2691 2775 3308 3675 4280 844
Size: 1424 31 392 84 533 367 605 3489
Fragments arranged by size:
3489 1424 605 533 392 367 84 31
BsrBI CCG'CTC
Cuts at: 1394 1823 2083 2411 3144 4812 1394
Size: 429 260 328 733 1668 3507
Fragments arranged by size:
3507 1668 733 429 328 260
BsrDI GCAATG_nn'
Cuts at: 65 1248 5420 5594 65
Size: 1183 4172 174 1396
Fragments arranged by size:
4172 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2365 3708 5569 5756 1700
Size: 110 555 1343 1861 187 2869
Fragments arranged by size:
2869 1861 1343 555 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6925
BssHII G'CGCG_C
Cuts at: 1925 2266 2604 3021 1925
Size: 341 338 417 5829
Fragments arranged by size:
5829 417 341 338
BssSI C'ACGA_G
Cuts at: 3469 4985 5997 3469
Size: 1516 1012 4397
Fragments arranged by size:
4397 1516 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6925
BstBI TT'CG_AA
Cuts at: 5729 5729
Size: 6925
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6925
BstXI CCAn_nnnn'nTGG
Cuts at: 3436 4099 3436
Size: 663 6262
BstYI r'GATC_y
Cuts at: 1972 2632 3035 3837 4255 4622 5007 5024 6476
Size: 660 403 802 418 367 385 17 1452
Cuts at: 6476 6562 6574 6920 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 802 660 418 403 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2708 2708
Size: 6925
BtgI C'CryG_G
Cuts at: 513 1065 2163 3777 3897 3989 4098 513
Size: 552 1098 1614 120 92 109 3340
Fragments arranged by size:
3340 1614 1098 552 120 109 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2320 2557 3288 487
Size: 683 56 1094 237 731 4124
Fragments arranged by size:
4124 1094 731 683 237 56
BtsI GCAGTG_nn'
Cuts at: 2125 2231 4441 5235 5255 2125
Size: 106 2210 794 20 3795
Fragments arranged by size:
3795 2210 794 106 20
ClaI AT'CG_AT
Cuts at: 1554 2918 1554
Size: 1364 5561
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6890
DraI TTT'AAA
Cuts at: 4229 4229
Size: 6925
DraIII CAC_nnn'GTG
Cuts at: 3018 4168 3018
Size: 1150 5775
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5932 809
Size: 507 4616 1802
Fragments arranged by size:
4616 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2290 2406 3263
Size: 54 1425 50 275 18 460 116 857
Cuts at: 3263 3438 3502 3963 4372 5261 6890 8
Size: 175 64 461 409 889 1629 43
Fragments arranged by size:
1629 1425 889 857 461 460 409 275
175 116 64 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4372 1537
Size: 275 2560 4090
Fragments arranged by size:
4090 2560 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 4855 1353
Size: 737 2765 3423
Fragments arranged by size:
3423 2765 737
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5512 5886 6032 387
Size: 1064 138 3923 374 146 1280
Fragments arranged by size:
3923 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2287 2287
Size: 6925
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2222 2318 2613 2756 3316 4024 4196
Size: 393 590 96 295 143 560 708 172
Cuts at: 4196 4988 5585 6372 6640 1239
Size: 792 597 787 268 1524
Fragments arranged by size:
1524 792 787 708 597 590 560 393
295 268 172 143 96
EcoO109I rG'GnC_Cy
Cuts at: 2077 2104 2105 2353 2848 2911 3229 3366 3455
Size: 27 1 248 495 63 318 137 89
Cuts at: 3455 2077
Size: 5547
Fragments arranged by size:
5547 495 318 248 137 89 63 27
1
EcoRI G'AATT_C
Cuts at: 4234 4612 4234
Size: 378 6547
EcoRV GAT'ATC
Cuts at: 3415 3415
Size: 6925
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4057 4057
Size: 6925
FspI TGC'GCA
Cuts at: 2237 5431 2237
Size: 3194 3731
HaeII r_GCGC'y
Cuts at: 1678 2209 2462 2486 3794 4153 6072 1678
Size: 531 253 24 1308 359 1919 2531
Fragments arranged by size:
2531 1919 1308 531 359 253 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5566 5598 5640 5672 1373
Size: 32 135 32 3994 32 42 32 2626
Fragments arranged by size:
3994 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4269 4515 669
Size: 988 281 2331 246 3079
Fragments arranged by size:
3079 2331 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6925
HpaI GTT'AAC
Cuts at: 4515 4515
Size: 6925
KpnI G_GTAC'C
Cuts at: 3165 3835 4250 3165
Size: 670 415 5840
Fragments arranged by size:
5840 670 415
MfeI C'AATT_G
Cuts at: 4524 4524
Size: 6925
MluI A'CGCG_T
Cuts at: 6801 6801
Size: 6925
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3120 6039 6223 1305
Size: 1815 2919 184 2007
Fragments arranged by size:
2919 2007 1815 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3440 10
Size: 54 1425 343 1608 3495
Fragments arranged by size:
3495 1608 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3273 3804 4100 4883 5242 5401 518
Size: 648 2107 531 296 783 359 159 2042
Fragments arranged by size:
2107 2042 783 648 531 359 296 159
MspA1I CmG'CkG
Cuts at: 1606 2023 2052 2165 2305 2404 2476 2640 2776
Size: 417 29 113 140 99 72 164 136
Cuts at: 2776 3029 3042 3076 3422 3726 4077 4204 4341
Size: 253 13 34 346 304 351 127 137
Cuts at: 4341 5018 6166 6411 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 417 351 346 304 253
245 164 140 137 136 127 113 99
72 34 29 13
NaeI GCC'GGC
Cuts at: 2367 2367
Size: 6925
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6373
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6925
NgoMIV G'CCGG_C
Cuts at: 2365 2365
Size: 6925
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6925
NotI GC'GGCC_GC
Cuts at: 4372 4372
Size: 6925
NspI r_CATG'y
Cuts at: 1132 1229 3389 4116 4283 5828 1132
Size: 97 2160 727 167 1545 2229
Fragments arranged by size:
2229 2160 1545 727 167 97
PasI CC'CwG_GG
Cuts at: 1348 2109 2563 3345 1348
Size: 761 454 782 4928
Fragments arranged by size:
4928 782 761 454
PciI A'CATG_T
Cuts at: 5824 5824
Size: 6925
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3877 4138 1108
Size: 2769 261 3895
Fragments arranged by size:
3895 2769 261
PfoI T'CCnGG_A
Cuts at: 2628 2628
Size: 6925
PmeI GTTT'AAAC
Cuts at: 4229 4229
Size: 6925
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2570 2602 4994 5026 6507 6539 2570
Size: 32 2392 32 1481 32 2956
Fragments arranged by size:
2956 2392 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2077 2848 2911 3366 2077
Size: 771 63 455 5636
Fragments arranged by size:
5636 771 455 63
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6925
PsiI TTA'TAA
Cuts at: 4495 4495
Size: 6925
PspOMI G'GGCC_C
Cuts at: 2104 2353 2429 3454 2104
Size: 249 76 1025 5575
Fragments arranged by size:
5575 1025 249 76
PstI C_TGCA'G
Cuts at: 830 2310 2490 2772 3672 3693 3762 4124 4149
Size: 1480 180 282 900 21 69 362 25
Cuts at: 4149 4277 830
Size: 128 3478
Fragments arranged by size:
3478 1480 900 362 282 180 128 69
25 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6925
PvuII CAG'CTG
Cuts at: 2052 2305 2404 2776 3029 3076 3422 2052
Size: 253 99 372 253 47 346 5555
Fragments arranged by size:
5555 372 346 253 253 99 47
SacI G_AGCT'C
Cuts at: 2289 2289
Size: 6925
SacII CC_GC'GG
Cuts at: 2166 2166
Size: 6925
SalI G'TCGA_C
Cuts at: 1936 4267 1936
Size: 2331 4594
SapI GCTCTTCn'nnn_
Cuts at: 2090 2090
Size: 6925
SbfI CC_TGCA'GG
Cuts at: 4277 4277
Size: 6925
ScaI AGT'ACT
Cuts at: 5173 6885 5173
Size: 1712 5213
SexAI A'CCwGG_T
Cuts at: 4164 4164
Size: 6925
SfcI C'TryA_G
Cuts at: 826 1045 2306 2486 2768 2843 3312 3583 3668
Size: 219 1261 180 282 75 469 271 85
Cuts at: 3668 3689 3758 4120 4145 4273 5408 6089 6280
Size: 21 69 362 25 128 1135 681 191
Cuts at: 6280 826
Size: 1471
Fragments arranged by size:
1471 1261 1135 681 469 362 282 271
219 191 180 128 85 75 69 25
21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6925
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6925
SmaI CCC'GGG
Cuts at: 1872 2358 2495 4252 1872
Size: 486 137 1757 4545
Fragments arranged by size:
4545 1757 486 137
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2872 3404 3979 5029 5930 6192
Size: 197 926 929 532 575 1050 901 262
Cuts at: 6192 820
Size: 1553
Fragments arranged by size:
1553 1050 929 926 901 575 532 262
197
SnaBI TAC'GTA
Cuts at: 493 4716 493
Size: 4223 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6925
SphI G_CATG'C
Cuts at: 1132 4283 1132
Size: 3151 3774
SspI AAT'ATT
Cuts at: 5 52 4849 5
Size: 47 4797 2081
Fragments arranged by size:
4797 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6925
StyI C'CwwG_G
Cuts at: 513 1065 2888 3225 4364 513
Size: 552 1823 337 1139 3074
Fragments arranged by size:
3074 1823 1139 552 337
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3141 4952 4969 5122 5307 5752 325
Size: 1255 1561 1811 17 153 185 445 1498
Fragments arranged by size:
1811 1561 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5171 6883 96
Size: 274 80 33 51 1443 3194 1712 138
Fragments arranged by size:
3194 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3314 3346 6885 6917 3314
Size: 32 3539 32 3322
Fragments arranged by size:
3539 3322 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6925
XbaI T'CTAG_A
Cuts at: 4261 4261
Size: 6925
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3448 3448
Size: 6925
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6925
XmaI C'CCGG_G
Cuts at: 1870 2356 2493 4250 1870
Size: 486 137 1757 4545
Fragments arranged by size:
4545 1757 486 137
XmnI GAAnn'nnTTC
Cuts at: 5054 5054
Size: 6925
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3637 4733 276
Size: 53 83 186 936 2103 1096 2468
Fragments arranged by size:
2468 2103 1096 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I
EcoNI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspXI PsrI RsrII SanDI SfiI SfoI SrfI
SwaI