Restriction map for
pFN21AB5736
(Circular) (Six-base) MAPSORT of: pFN21AB5736.seq Check: 9291 from: 1 to: 6913
pFN21AB5736 6913 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2950 2950
Size: 6913
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2756 4723 278
Size: 53 83 186 936 1220 1967 2468
Fragments arranged by size:
2468 1967 1220 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3785 4256 1937
Size: 1848 471 4594
Fragments arranged by size:
4594 1848 471
Acc65I G'GTAC_C
Cuts at: 4234 4234
Size: 6913
AclI AA'CG_TT
Cuts at: 1563 5040 5413 1563
Size: 3477 373 3063
Fragments arranged by size:
3477 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2746 3579 4976 6360 6628 1632
Size: 1114 833 1397 1384 268 1917
Fragments arranged by size:
1917 1397 1384 1114 833 268
AflII C'TTAA_G
Cuts at: 820 1017 2702 820
Size: 197 1685 5031
Fragments arranged by size:
5031 1685 197
AflIII A'CryG_T
Cuts at: 1203 5812 6789 1203
Size: 4609 977 1327
Fragments arranged by size:
4609 1327 977
AgeI A'CCGG_T
Cuts at: 5744 5744
Size: 6913
AhdI GACnn_n'nnGTC
Cuts at: 3317 3476 5642 3317
Size: 159 2166 4588
Fragments arranged by size:
4588 2166 159
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6913
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2703 1184
Size: 1519 5394
AlwNI CAG_nnn'CTG
Cuts at: 3308 4656 6228 3308
Size: 1348 1572 3993
Fragments arranged by size:
3993 1572 1348
ApaI G_GGCC'C
Cuts at: 3838 3938 4108 3838
Size: 100 170 6643
Fragments arranged by size:
6643 170 100
ApaLI G'TGCA_C
Cuts at: 3026 4970 6126 3026
Size: 1944 1156 3813
Fragments arranged by size:
3813 1944 1156
ApoI r'AATT_y
Cuts at: 1493 2214 4222 4448 4600 6709 1493
Size: 721 2008 226 152 2109 1697
Fragments arranged by size:
2109 2008 1697 721 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5467 160
Size: 872 4435 1606
Fragments arranged by size:
4435 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6913
AvaI C'yCGr_G
Cuts at: 1870 1943 2294 2322 2369 3824 3839 4238 1870
Size: 73 351 28 47 1455 15 399 4545
Fragments arranged by size:
4545 1455 399 351 73 47 28 15
BamHI G'GATC_C
Cuts at: 3070 4243 4610 3070
Size: 1173 367 5373
Fragments arranged by size:
5373 1173 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2159 2335 3167 3965 4234
Size: 325 217 627 372 176 832 798 269
Cuts at: 4234 5689 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 832 798 627 372 325 269
217 176
BanII G_rGCy'C
Cuts at: 1391 2588 3114 3371 3838 3876 3938 3985 3996
Size: 1197 526 257 467 38 62 47 11
Cuts at: 3996 4108 4130 4232 1391
Size: 112 22 102 4072
Fragments arranged by size:
4072 1197 526 467 257 112 102 62
47 38 22 11
BbeI G_GCGC'C
Cuts at: 2163 2339 2163
Size: 176 6737
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3508 928
Size: 971 1609 4333
Fragments arranged by size:
4333 1609 971
BbvCI CC'TCA_GC
Cuts at: 3014 3014
Size: 6913
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2104 2138 5104 5138 1903
Size: 34 167 34 2966 34 3678
Fragments arranged by size:
3678 2966 167 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2272 2570 3598 4805 6015 2272
Size: 298 1028 1207 1210 3170
Fragments arranged by size:
3170 1210 1207 1028 298
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6913
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3888 5524 136
Size: 107 122 71 631 605 2216 1636 1525
Fragments arranged by size:
2216 1636 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6908 6908
Size: 6913
BlpI GC'TnA_GC
Cuts at: 4330 4330
Size: 6913
Bme1580I G_kGCm'C
Cuts at: 1790 3030 3838 3938 3970 4108 4137 4974 6130
Size: 1240 808 100 32 138 29 837 1156
Cuts at: 6130 1790
Size: 2573
Fragments arranged by size:
2573 1240 1156 837 808 138 100 32
29
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6913
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2031 3969 4644
Size: 416 53 83 399 24 614 1938 675
Cuts at: 4644 5602 442
Size: 958 1753
Fragments arranged by size:
1938 1753 958 675 614 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6913
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2605 2605
Size: 6913
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2292 2325 2465 2579 2633 2765 3285 3568
Size: 1053 33 140 114 54 132 520 283
Cuts at: 3568 3862 4059 4068 4095 5573 1239
Size: 294 197 9 27 1478 2579
Fragments arranged by size:
2579 1478 1053 520 294 283 197 140
132 114 54 33 27 9
Bpu10I CC'TnA_GC
Cuts at: 3014 3414 3014
Size: 400 6513
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2380 2536 3323 5038 5903 6201 2380
Size: 156 787 1715 865 298 3092
Fragments arranged by size:
3092 1715 865 787 298 156
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3257 3516 4113 5576 882
Size: 617 1758 259 597 1463 2219
Fragments arranged by size:
2219 1758 1463 617 597 259
BsaAI yAC'GTr
Cuts at: 493 1204 3400 4704 493
Size: 711 2196 1304 2702
Fragments arranged by size:
2702 2196 1304 711
BsaBI GATnn'nnATC
Cuts at: 3347 6907 3347
Size: 3560 3353
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2160 2336 2753 4720
Size: 53 83 186 936 627 176 417 1967
Cuts at: 4720 5102 5737 275
Size: 382 635 1451
Fragments arranged by size:
1967 1451 936 635 627 417 382 186
176 83 53
BsaWI w'CCGG_w
Cuts at: 5346 5744 6018 6165 6576 5346
Size: 398 274 147 411 5683
Fragments arranged by size:
5683 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2570 2600 2738 2768 2879
Size: 30 165 30 1167 30 138 30 111
Cuts at: 2879 2909 3566 3596 3598 3628 1178
Size: 30 657 30 2 30 4463
Fragments arranged by size:
4463 1167 657 165 138 111 30 30
30 30 30 30 30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2578 2657 3069 3184 3606 3842 3930 1186
Size: 1392 79 412 115 422 236 88 4169
Fragments arranged by size:
4169 1392 422 412 236 115 88 79
BseYI C'CCAG_C
Cuts at: 2525 3066 3175 4187 6116 6612 2525
Size: 541 109 1012 1929 496 2826
Fragments arranged by size:
2826 1929 1012 541 496 109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4112 1163
Size: 573 2376 3964
Fragments arranged by size:
3964 2376 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2925 4363 5124 6152 1540
Size: 275 186 924 1438 761 1028 2301
Fragments arranged by size:
2301 1438 1028 924 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2588 3030 3206 4974 5059 6130 2588
Size: 442 176 1768 85 1071 3371
Fragments arranged by size:
3371 1768 1071 442 176 85
BsmI GAATG_Cn'
Cuts at: 4424 4517 4424
Size: 93 6820
BsmBI CGTCTCn'nnnn_
Cuts at: 2036 2868 2036
Size: 832 6081
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2588 3030 3114 3206 3371 3838 3876
Size: 399 798 442 84 92 165 467 38
Cuts at: 3876 3938 3970 3985 3996 4108 4130 4137 4232
Size: 62 32 15 11 112 22 7 95
Cuts at: 4232 4974 5059 6130 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 798 742 467 442 399 165
112 95 92 85 84 62 38 32
22 15 11 7
BspEI T'CCGG_A
Cuts at: 6576 6576
Size: 6913
BspHI T'CATG_A
Cuts at: 2598 2901 4053 4802 6532 2598
Size: 303 1152 749 1730 2979
Fragments arranged by size:
2979 1730 1152 749 303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2649 2950 4268 844
Size: 1805 301 1318 3489
Fragments arranged by size:
3489 1805 1318 301
BsrBI CCG'CTC
Cuts at: 1394 1823 2491 2869 3418 3832 4800 1394
Size: 429 668 378 549 414 968 3507
Fragments arranged by size:
3507 968 668 549 429 414 378
BsrDI GCAATG_nn'
Cuts at: 65 1248 2869 5408 5582 65
Size: 1183 1621 2539 174 1396
Fragments arranged by size:
2539 1621 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2712 5557 5744 1700
Size: 110 902 2845 187 2869
Fragments arranged by size:
2869 2845 902 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6913
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6913
BssSI C'ACGA_G
Cuts at: 4973 5985 4973
Size: 1012 5901
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6913
BstBI TT'CG_AA
Cuts at: 5717 5717
Size: 6913
BstEII G'GTnAC_C
Cuts at: 1184 3280 1184
Size: 2096 4817
BstXI CCAn_nnnn'nTGG
Cuts at: 2540 2611 3292 4004 4048 2540
Size: 71 681 712 44 5405
Fragments arranged by size:
5405 712 681 71 44
BstYI r'GATC_y
Cuts at: 1972 2094 3070 4243 4610 4995 5012 6464 6550
Size: 122 976 1173 367 385 17 1452 86
Cuts at: 6550 6562 6908 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1173 976 385 367 346 122
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3081 3081
Size: 6913
BtgI C'CryG_G
Cuts at: 513 1065 2004 2154 2673 3153 3291 4176 513
Size: 552 939 150 519 480 138 885 3250
Fragments arranged by size:
3250 939 885 552 519 480 150 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3942 487
Size: 683 56 2716 3458
Fragments arranged by size:
3458 2716 683 56
BtsI GCAGTG_nn'
Cuts at: 2518 2692 3509 3748 3784 3862 3868 4095 4429
Size: 174 817 239 36 78 6 227 334
Cuts at: 4429 5223 5243 2518
Size: 794 20 4188
Fragments arranged by size:
4188 817 794 334 239 227 174 78
36 20 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6913
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6878
DraI TTT'AAA
Cuts at: 4217 4217
Size: 6913
DraIII CAC_nnn'GTG
Cuts at: 3794 3794
Size: 6913
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5920 809
Size: 507 4604 1802
Fragments arranged by size:
4604 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2571 2854 2922
Size: 54 1425 50 275 18 741 283 68
Cuts at: 2922 3057 4173 4360 5249 6878 8
Size: 135 1116 187 889 1629 43
Fragments arranged by size:
1629 1425 1116 889 741 283 275 187
135 68 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2922 4360 1537
Size: 275 1110 1438 4090
Fragments arranged by size:
4090 1438 1110 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2067 4843 1353
Size: 714 2776 3423
Fragments arranged by size:
3423 2776 714
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5500 5874 6020 387
Size: 1064 138 3911 374 146 1280
Fragments arranged by size:
3911 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2586 2586
Size: 6913
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2292 2325 2465 2579 2633 2746 2765
Size: 393 660 33 140 114 54 113 19
Cuts at: 2765 3285 3568 3579 3862 4059 4068 4095 4976
Size: 520 283 11 283 197 9 27 881
Cuts at: 4976 5573 6360 6628 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 881 787 660 597 520 393 283
283 268 197 140 114 113 54 33
27 19 11 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2066 2066
Size: 6913
EcoO109I rG'GnC_Cy
Cuts at: 3835 3934 3935 4104 4105 3835
Size: 99 1 169 1 6643
Fragments arranged by size:
6643 169 99 1 1
EcoRI G'AATT_C
Cuts at: 4222 4600 4222
Size: 378 6535
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2848 3508 2848
Size: 660 6253
FspI TGC'GCA
Cuts at: 5419 5419
Size: 6913
HaeII r_GCGC'y
Cuts at: 1678 2163 2339 2526 2566 2710 6060 1678
Size: 485 176 187 40 144 3350 2531
Fragments arranged by size:
3350 2531 485 187 176 144 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2290 2322 2608 2640 2738
Size: 32 135 32 718 32 286 32 98
Cuts at: 2738 2770 3260 3292 3303 3335 3462 3494 3704
Size: 32 490 32 11 32 127 32 210
Cuts at: 3704 3736 3833 3865 4112 4144 5554 5586 5628
Size: 32 97 32 247 32 1410 32 42
Cuts at: 5628 5660 1373
Size: 32 2626
Fragments arranged by size:
2626 1410 718 490 286 247 210 135
127 98 97 42 32 32 32 32
32 32 32 32 32 32 32 32
32 11
HincII GTy'rAC
Cuts at: 669 1657 1938 3382 3481 3556 3786 4257 4503
Size: 988 281 1444 99 75 230 471 246
Cuts at: 4503 669
Size: 3079
Fragments arranged by size:
3079 1444 988 471 281 246 230 99
75
HindIII A'AGCT_T
Cuts at: 748 4162 748
Size: 3414 3499
HpaI GTT'AAC
Cuts at: 4503 4503
Size: 6913
KasI G'GCGC_C
Cuts at: 2159 2335 2159
Size: 176 6737
KpnI G_GTAC'C
Cuts at: 4238 4238
Size: 6913
MfeI C'AATT_G
Cuts at: 3123 4512 3123
Size: 1389 5524
MluI A'CGCG_T
Cuts at: 6789 6789
Size: 6913
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3493 6027 6211 1305
Size: 2188 2534 184 2007
Fragments arranged by size:
2534 2188 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2856 3059 4175 10
Size: 54 1425 343 1024 203 1116 2748
Fragments arranged by size:
2748 1425 1116 1024 343 203 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2064 2418 4002 4046 4175 4871 5230
Size: 648 898 354 1584 44 129 696 359
Cuts at: 5230 5389 518
Size: 159 2042
Fragments arranged by size:
2042 1584 898 696 648 359 354 159
129 44
MspA1I CmG'CkG
Cuts at: 1606 2149 2156 2382 3155 3444 4329 5006 6154
Size: 543 7 226 773 289 885 677 1148
Cuts at: 6154 6399 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 885 773 677 543 289 245
226 7
NarI GG'CG_CC
Cuts at: 2160 2336 2160
Size: 176 6737
NcoI C'CATG_G
Cuts at: 513 1065 2004 3291 4176 513
Size: 552 939 1287 885 3250
Fragments arranged by size:
3250 1287 939 885 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6913
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6913
NotI GC'GGCC_GC
Cuts at: 4360 4360
Size: 6913
NspI r_CATG'y
Cuts at: 1132 1229 2953 3334 4271 5816 1132
Size: 97 1724 381 937 1545 2229
Fragments arranged by size:
2229 1724 1545 937 381 97
PasI CC'CwG_GG
Cuts at: 1348 3989 1348
Size: 2641 4272
PciI A'CATG_T
Cuts at: 5812 5812
Size: 6913
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3066 3308 3400 3636 1108
Size: 1958 242 92 236 4385
Fragments arranged by size:
4385 1958 242 236 92
PfoI T'CCnGG_A
Cuts at: 3321 3321
Size: 6913
PmeI GTTT'AAAC
Cuts at: 4217 4217
Size: 6913
PmlI CAC'GTG
Cuts at: 3400 3400
Size: 6913
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4982 5014 6495 6527 4982
Size: 32 1481 32 5368
Fragments arranged by size:
5368 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6913
PsiI TTA'TAA
Cuts at: 4483 4483
Size: 6913
PspOMI G'GGCC_C
Cuts at: 3834 3934 4104 3834
Size: 100 170 6643
Fragments arranged by size:
6643 170 100
PstI C_TGCA'G
Cuts at: 830 2148 4265 830
Size: 1318 2117 3478
Fragments arranged by size:
3478 2117 1318
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6913
SacI G_AGCT'C
Cuts at: 2588 2588
Size: 6913
SacII CC_GC'GG
Cuts at: 2157 3156 2157
Size: 999 5914
SalI G'TCGA_C
Cuts at: 1936 3784 4255 1936
Size: 1848 471 4594
Fragments arranged by size:
4594 1848 471
SbfI CC_TGCA'GG
Cuts at: 4265 4265
Size: 6913
ScaI AGT'ACT
Cuts at: 2168 5161 6873 2168
Size: 2993 1712 2208
Fragments arranged by size:
2993 2208 1712
SexAI A'CCwGG_T
Cuts at: 2085 2085
Size: 6913
SfcI C'TryA_G
Cuts at: 826 1045 2144 3685 4261 5396 6077 6268 826
Size: 219 1099 1541 576 1135 681 191 1471
Fragments arranged by size:
1541 1471 1135 1099 681 576 219 191
SfoI GGC'GCC
Cuts at: 2161 2337 2161
Size: 176 6737
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6913
SgrAI Cr'CCGG_yG
Cuts at: 1700 2712 1700
Size: 1012 5901
SmaI CCC'GGG
Cuts at: 1872 4240 1872
Size: 2368 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2395 2551 2702 3338 5017 5918
Size: 197 926 452 156 151 636 1679 901
Cuts at: 5918 6180 820
Size: 262 1553
Fragments arranged by size:
1679 1553 926 901 636 452 262 197
156 151
SnaBI TAC'GTA
Cuts at: 493 4704 493
Size: 4211 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6913
SphI G_CATG'C
Cuts at: 1132 4271 1132
Size: 3139 3774
SspI AAT'ATT
Cuts at: 5 52 4837 5
Size: 47 4785 2081
Fragments arranged by size:
4785 2081 47
StuI AGG'CCT
Cuts at: 1517 2401 3086 3462 1517
Size: 884 685 376 4968
Fragments arranged by size:
4968 884 685 376
StyI C'CwwG_G
Cuts at: 513 1065 2004 3285 3291 4176 4352 513
Size: 552 939 1281 6 885 176 3074
Fragments arranged by size:
3074 1281 939 885 552 176 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3130 4002 4940 4957 5110 5295 5740
Size: 1255 1550 872 938 17 153 185 445
Cuts at: 5740 325
Size: 1498
Fragments arranged by size:
1550 1498 1255 938 872 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2166 2826 5159
Size: 274 80 33 51 1443 189 660 2333
Cuts at: 5159 6871 96
Size: 1712 138
Fragments arranged by size:
2333 1712 1443 660 274 189 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3317 3349 4062 4094 6873 6905 3317
Size: 32 713 32 2779 32 3325
Fragments arranged by size:
3325 2779 713 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6913
XbaI T'CTAG_A
Cuts at: 4249 4249
Size: 6913
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2540 2540
Size: 6913
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6913
XmaI C'CCGG_G
Cuts at: 1870 4238 1870
Size: 2368 4545
XmnI GAAnn'nnTTC
Cuts at: 5042 5042
Size: 6913
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2754 4721 276
Size: 53 83 186 936 1220 1967 2468
Fragments arranged by size:
2468 1967 1220 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PstI PvuI SacI SacII
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI
PpuMI PspXI PsrI PvuII RsrII SanDI SapI SfiI
SrfI SwaI