Restriction map for
pFN21AB5733
(Circular) (Six-base) MAPSORT of: pFN21AB5733.seq Check: 5020 from: 1 to: 7087
pFN21AB5733 7087 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2290 4149 2290
Size: 1859 5228
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4897 278
Size: 53 83 186 936 3361 2468
Fragments arranged by size:
3361 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4430 1937
Size: 2493 4594
Acc65I G'GTAC_C
Cuts at: 2370 4408 2370
Size: 2038 5049
AclI AA'CG_TT
Cuts at: 1563 5214 5587 1563
Size: 3651 373 3063
Fragments arranged by size:
3651 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4317 5150 6534 6802 1632
Size: 2685 833 1384 268 1917
Fragments arranged by size:
2685 1917 1384 833 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6890
AflIII A'CryG_T
Cuts at: 1203 2100 2328 5986 6963 1203
Size: 897 228 3658 977 1327
Fragments arranged by size:
3658 1327 977 897 228
AgeI A'CCGG_T
Cuts at: 5918 5918
Size: 7087
AhdI GACnn_n'nnGTC
Cuts at: 5816 5816
Size: 7087
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7087
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7087
AlwNI CAG_nnn'CTG
Cuts at: 2290 3113 3762 3924 4830 6402 2290
Size: 823 649 162 906 1572 2975
Fragments arranged by size:
2975 1572 906 823 649 162
ApaI G_GGCC'C
Cuts at: 2771 2771
Size: 7087
ApaLI G'TGCA_C
Cuts at: 3082 5144 6300 3082
Size: 2062 1156 3869
Fragments arranged by size:
3869 2062 1156
ApoI r'AATT_y
Cuts at: 1493 4396 4622 4774 6883 1493
Size: 2903 226 152 2109 1697
Fragments arranged by size:
2903 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5641 160
Size: 872 4609 1606
Fragments arranged by size:
4609 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7087
AvaI C'yCGr_G
Cuts at: 1870 1943 2771 2881 3398 4412 1870
Size: 73 828 110 517 1014 4545
Fragments arranged by size:
4545 1014 828 517 110 73
BamHI G'GATC_C
Cuts at: 3628 4417 4784 3628
Size: 789 367 5931
Fragments arranged by size:
5931 789 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2370 3101 3299 3533 3719
Size: 325 217 627 583 731 198 234 186
Cuts at: 3719 4408 5863 618
Size: 689 1455 1842
Fragments arranged by size:
1842 1455 731 689 627 583 325 234
217 198 186
BanII G_rGCy'C
Cuts at: 1391 2343 2771 2864 3378 3438 4406 1391
Size: 952 428 93 514 60 968 4072
Fragments arranged by size:
4072 968 952 514 428 93 60
BbeI G_GCGC'C
Cuts at: 3303 3303
Size: 7087
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2083 3416 4180 4192 928
Size: 971 184 1333 764 12 3823
Fragments arranged by size:
3823 1333 971 764 184 12
BbvCI CC'TCA_GC
Cuts at: 2012 2361 3106 3983 4163 2012
Size: 349 745 877 180 4936
Fragments arranged by size:
4936 877 745 349 180
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3052 3086 4061 4095 5278 5312 1903
Size: 34 1115 34 975 34 1183 34 3678
Fragments arranged by size:
3678 1183 1115 975 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4979 6189 4979
Size: 1210 5877
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7087
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2819 3308 3531
Size: 107 122 71 631 605 1147 489 223
Cuts at: 3531 3852 4081 5698 136
Size: 321 229 1617 1525
Fragments arranged by size:
1617 1525 1147 631 605 489 321 229
223 122 107 71
BglII A'GATC_T
Cuts at: 2181 7082 2181
Size: 4901 2186
BlpI GC'TnA_GC
Cuts at: 4504 4504
Size: 7087
Bme1580I G_kGCm'C
Cuts at: 1790 2439 2771 3086 3104 3536 4319 5148 6304
Size: 649 332 315 18 432 783 829 1156
Cuts at: 6304 1790
Size: 2573
Fragments arranged by size:
2573 1156 829 783 649 432 332 315
18
BmgBI CAC'GTC
Cuts at: 1318 2329 3777 3901 1318
Size: 1011 1448 124 4504
Fragments arranged by size:
4504 1448 1011 124
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3244 3604 4818
Size: 416 53 83 399 24 1827 360 1214
Cuts at: 4818 5776 442
Size: 958 1753
Fragments arranged by size:
1827 1753 1214 958 416 399 360 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2066 1056
Size: 1010 6077
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2292 2734 3019 3649 2292
Size: 442 285 630 5730
Fragments arranged by size:
5730 630 442 285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2142 3170 3476 3732 3834 5747 1239
Size: 903 1028 306 256 102 1913 2579
Fragments arranged by size:
2579 1913 1028 903 306 256 102
Bpu10I CC'TnA_GC
Cuts at: 2012 2076 2361 3106 3121 3465 3983 4163 2012
Size: 64 285 745 15 344 518 180 4936
Fragments arranged by size:
4936 745 518 344 285 180 64 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2262 5212 6077 6375 2262
Size: 2950 865 298 2974
Fragments arranged by size:
2974 2950 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2455 3899 5750 882
Size: 617 956 1444 1851 2219
Fragments arranged by size:
2219 1851 1444 956 617
BsaAI yAC'GTr
Cuts at: 493 1204 2103 4878 493
Size: 711 899 2775 2702
Fragments arranged by size:
2775 2702 899 711
BsaBI GATnn'nnATC
Cuts at: 3627 7081 3627
Size: 3454 3633
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3300 4894 5276 5911
Size: 53 83 186 936 1767 1594 382 635
Cuts at: 5911 275
Size: 1451
Fragments arranged by size:
1767 1594 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5520 5918 6192 6339 6750 5520
Size: 398 274 147 411 5857
Fragments arranged by size:
5857 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4304 4334 1178
Size: 30 165 30 2901 30 3931
Fragments arranged by size:
3931 2901 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2229 2831 4325 1186
Size: 1043 602 1494 3948
Fragments arranged by size:
3948 1494 1043 602
BseYI C'CCAG_C
Cuts at: 2128 2550 2722 2763 2857 3342 3992 6290 6786
Size: 422 172 41 94 485 650 2298 496
Cuts at: 6786 2128
Size: 2429
Fragments arranged by size:
2429 2298 650 496 485 422 172 94
41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3803 1163
Size: 573 2067 4447
Fragments arranged by size:
4447 2067 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4537 5298 6326 1540
Size: 275 186 2536 761 1028 2301
Fragments arranged by size:
2536 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3086 3462 5148 5233 6304 3086
Size: 376 1686 85 1071 3869
Fragments arranged by size:
3869 1686 1071 376 85
BsmI GAATG_Cn'
Cuts at: 4598 4691 4598
Size: 93 6994
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2343 2439 2771 2864 3086 3104 3378
Size: 399 553 96 332 93 222 18 274
Cuts at: 3378 3438 3462 3536 4319 4406 5148 5233 6304
Size: 60 24 74 783 87 742 85 1071
Cuts at: 6304 1391
Size: 2174
Fragments arranged by size:
2174 1071 783 742 553 399 332 274
222 96 93 87 85 74 60 24
18
BspEI T'CCGG_A
Cuts at: 6750 6750
Size: 7087
BspHI T'CATG_A
Cuts at: 3603 4976 6706 3603
Size: 1373 1730 3984
Fragments arranged by size:
3984 1730 1373
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2290 4149 4442 844
Size: 1446 1859 293 3489
Fragments arranged by size:
3489 1859 1446 293
BsrBI CCG'CTC
Cuts at: 1394 1823 3540 4974 1394
Size: 429 1717 1434 3507
Fragments arranged by size:
3507 1717 1434 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3135 5582 5756 65
Size: 1183 1887 2447 174 1396
Fragments arranged by size:
2447 1887 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2259 3128 3964 4157 5731 5918 1700
Size: 110 449 869 836 193 1574 187 2869
Fragments arranged by size:
2869 1574 869 836 449 193 187 110
BsrGI T'GTAC_A
Cuts at: 96 4251 96
Size: 4155 2932
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7087
BssSI C'ACGA_G
Cuts at: 5147 6159 5147
Size: 1012 6075
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2072 3762 4114 794
Size: 1278 1690 352 3767
Fragments arranged by size:
3767 1690 1278 352
BstBI TT'CG_AA
Cuts at: 5891 5891
Size: 7087
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7087
BstXI CCAn_nnnn'nTGG
Cuts at: 2146 3250 3275 3517 3550 2146
Size: 1104 25 242 33 5683
Fragments arranged by size:
5683 1104 242 33 25
BstYI r'GATC_y
Cuts at: 1972 2181 3628 4417 4784 5169 5186 6638 6724
Size: 209 1447 789 367 385 17 1452 86
Cuts at: 6724 6736 7082 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1447 789 385 367 346 209
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3729 4146 3729
Size: 417 6670
BtgI C'CryG_G
Cuts at: 513 1065 2004 2249 2830 2984 3303 3496 3528
Size: 552 939 245 581 154 319 193 32
Cuts at: 3528 4257 4264 513
Size: 729 7 3336
Fragments arranged by size:
3336 939 729 581 552 319 245 193
154 32 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6348
Fragments arranged by size:
6348 683 56
BtsI GCAGTG_nn'
Cuts at: 2372 2712 3037 3763 3841 4603 5397 5417 2372
Size: 340 325 726 78 762 794 20 4042
Fragments arranged by size:
4042 794 762 726 340 325 78 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7087
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2267 2302 2953 2988 529
Size: 35 1703 35 651 35 4628
Fragments arranged by size:
4628 1703 651 35 35 35
DraI TTT'AAA
Cuts at: 4391 4391
Size: 7087
DraIII CAC_nnn'GTG
Cuts at: 2595 2597 3193 2595
Size: 2 596 6489
Fragments arranged by size:
6489 596 2
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2227 3775 6094 809
Size: 507 911 1548 2319 1802
Fragments arranged by size:
2319 1802 1548 911 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2136 2619 3015
Size: 54 1425 50 275 18 306 483 396
Cuts at: 3015 3507 4155 4534 5423 7052 8
Size: 492 648 379 889 1629 43
Fragments arranged by size:
1629 1425 889 648 492 483 396 379
306 275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4534 1537
Size: 275 2722 4090
Fragments arranged by size:
4090 2722 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3821 4326 5017 1353
Size: 2468 505 691 3423
Fragments arranged by size:
3423 2468 691 505
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5674 6048 6194 387
Size: 1064 138 4085 374 146 1280
Fragments arranged by size:
4085 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2142 3170 3476 3732 3834 4317 5150
Size: 393 510 1028 306 256 102 483 833
Cuts at: 5150 5747 6534 6802 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1028 833 787 597 510 483 393
306 268 256 102
EcoNI CCTnn'n_nnAGG
Cuts at: 2813 4145 2813
Size: 1332 5755
EcoO109I rG'GnC_Cy
Cuts at: 2016 2395 2539 2767 2768 3646 2016
Size: 379 144 228 1 878 5457
Fragments arranged by size:
5457 878 379 228 144 1
EcoRI G'AATT_C
Cuts at: 4396 4774 4396
Size: 378 6709
FseI GG_CCGG'CC
Cuts at: 3968 4161 3968
Size: 193 6894
FspI TGC'GCA
Cuts at: 2114 2678 5593 2114
Size: 564 2915 3608
Fragments arranged by size:
3608 2915 564
FspAI rTGC'GCAy
Cuts at: 2114 2678 2114
Size: 564 6523
HaeII r_GCGC'y
Cuts at: 1678 2964 3303 4383 6234 1678
Size: 1286 339 1080 1851 2531
Fragments arranged by size:
2531 1851 1286 1080 339
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4304 4336 5728 5760 5802
Size: 32 135 32 2732 32 1392 32 42
Cuts at: 5802 5834 1373
Size: 32 2626
Fragments arranged by size:
2732 2626 1392 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4431 4677 669
Size: 988 281 2493 246 3079
Fragments arranged by size:
3079 2493 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7087
HpaI GTT'AAC
Cuts at: 4677 4677
Size: 7087
KasI G'GCGC_C
Cuts at: 3299 3299
Size: 7087
KpnI G_GTAC'C
Cuts at: 2374 4412 2374
Size: 2038 5049
MfeI C'AATT_G
Cuts at: 4686 4686
Size: 7087
MluI A'CGCG_T
Cuts at: 6963 6963
Size: 7087
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6201 6385 1305
Size: 4896 184 2007
Fragments arranged by size:
4896 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2138 2621 3017 3509 10
Size: 54 1425 343 306 483 396 492 3588
Fragments arranged by size:
3588 1425 492 483 396 343 306 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2567 3222 3273 5045 5404 5563 518
Size: 648 1401 655 51 1772 359 159 2042
Fragments arranged by size:
2042 1772 1401 655 648 359 159 51
MspA1I CmG'CkG
Cuts at: 1606 2069 2132 2251 2392 2763 2910 3346 3987
Size: 463 63 119 141 371 147 436 641
Cuts at: 3987 4167 4216 4503 5180 6328 6573 1606
Size: 180 49 287 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 641 463 436 371 287
245 180 147 141 119 63 49
NaeI GCC'GGC
Cuts at: 2261 3130 3966 4159 2261
Size: 869 836 193 5189
Fragments arranged by size:
5189 869 836 193
NarI GG'CG_CC
Cuts at: 3300 3300
Size: 7087
NcoI C'CATG_G
Cuts at: 513 1065 2004 3303 3528 4257 513
Size: 552 939 1299 225 729 3343
Fragments arranged by size:
3343 1299 939 729 552 225
NdeI CA'TA_TG
Cuts at: 387 3274 387
Size: 2887 4200
NgoMIV G'CCGG_C
Cuts at: 2259 3128 3964 4157 2259
Size: 869 836 193 5189
Fragments arranged by size:
5189 869 836 193
NheI G'CTAG_C
Cuts at: 1052 2062 1052
Size: 1010 6077
NotI GC'GGCC_GC
Cuts at: 4534 4534
Size: 7087
NspI r_CATG'y
Cuts at: 1132 1229 2683 3184 3957 4445 5990 1132
Size: 97 1454 501 773 488 1545 2229
Fragments arranged by size:
2229 1545 1454 773 501 488 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7087
PciI A'CATG_T
Cuts at: 5986 5986
Size: 7087
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2103 3234 1108
Size: 995 1131 4961
Fragments arranged by size:
4961 1131 995
PmeI GTTT'AAAC
Cuts at: 4391 4391
Size: 7087
PmlI CAC'GTG
Cuts at: 2103 2103
Size: 7087
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5156 5188 6669 6701 5156
Size: 32 1481 32 5542
Fragments arranged by size:
5542 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2395 2539 3646 2395
Size: 144 1107 5836
Fragments arranged by size:
5836 1107 144
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7087
PsiI TTA'TAA
Cuts at: 4657 4657
Size: 7087
PspOMI G'GGCC_C
Cuts at: 2767 2767
Size: 7087
PspXI vC'TCGA_Gb
Cuts at: 3398 3398
Size: 7087
PstI C_TGCA'G
Cuts at: 830 2268 4215 4439 830
Size: 1438 1947 224 3478
Fragments arranged by size:
3478 1947 1438 224
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7087
PvuII CAG'CTG
Cuts at: 2069 2763 2910 3987 4167 4216 2069
Size: 694 147 1077 180 49 4940
Fragments arranged by size:
4940 1077 694 180 147 49
SacII CC_GC'GG
Cuts at: 2252 2252
Size: 7087
SalI G'TCGA_C
Cuts at: 1936 4429 1936
Size: 2493 4594
SapI GCTCTTCn'nnn_
Cuts at: 3821 3821
Size: 7087
SbfI CC_TGCA'GG
Cuts at: 4439 4439
Size: 7087
ScaI AGT'ACT
Cuts at: 5335 7047 5335
Size: 1712 5375
SexAI A'CCwGG_T
Cuts at: 3189 3516 3189
Size: 327 6760
SfcI C'TryA_G
Cuts at: 826 1045 2264 2752 3025 3883 4211 4435 5570
Size: 219 1219 488 273 858 328 224 1135
Cuts at: 5570 6251 6442 826
Size: 681 191 1471
Fragments arranged by size:
1471 1219 1135 858 681 488 328 273
224 219 191
SfoI GGC'GCC
Cuts at: 3301 3301
Size: 7087
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7087
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7087
SmaI CCC'GGG
Cuts at: 1872 4414 1872
Size: 2542 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2277 3398 5191 6092 6354 820
Size: 197 926 334 1121 1793 901 262 1553
Fragments arranged by size:
1793 1553 1121 926 901 334 262 197
SnaBI TAC'GTA
Cuts at: 493 4878 493
Size: 4385 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7087
SphI G_CATG'C
Cuts at: 1132 2683 4445 1132
Size: 1551 1762 3774
Fragments arranged by size:
3774 1762 1551
SspI AAT'ATT
Cuts at: 5 52 5011 5
Size: 47 4959 2081
Fragments arranged by size:
4959 2081 47
StuI AGG'CCT
Cuts at: 1517 2822 2999 3788 1517
Size: 1305 177 789 4816
Fragments arranged by size:
4816 1305 789 177
StyI C'CwwG_G
Cuts at: 513 1065 2004 2052 3303 3528 4005 4257 4526
Size: 552 939 48 1251 225 477 252 269
Cuts at: 4526 513
Size: 3074
Fragments arranged by size:
3074 1251 939 552 477 269 252 225
48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2020 5114 5131 5284 5469 5914 325
Size: 1255 440 3094 17 153 185 445 1498
Fragments arranged by size:
3094 1498 1255 445 440 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2281 4251 5333
Size: 274 80 33 51 1443 304 1970 1082
Cuts at: 5333 7045 96
Size: 1712 138
Fragments arranged by size:
1970 1712 1443 1082 304 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7047 7079 7047
Size: 32 7055
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7087
XbaI T'CTAG_A
Cuts at: 4423 4423
Size: 7087
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3247 3310 3247
Size: 63 7024
XhoI C'TCGA_G
Cuts at: 1943 3398 1943
Size: 1455 5632
XmaI C'CCGG_G
Cuts at: 1870 4412 1870
Size: 2542 4545
XmnI GAAnn'nnTTC
Cuts at: 2090 5216 2090
Size: 3126 3961
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4895 276
Size: 53 83 186 936 3361 2468
Fragments arranged by size:
3361 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I EcoICRI EcoRV FalI NruI NsiI PacI PfoI
PsrI RsrII SacI SanDI SfiI SrfI SwaI