Restriction map for
pFN21AB5730
(Circular) (Six-base) MAPSORT of: pFN21AB5730.seq Check: 4624 from: 1 to: 6901
pFN21AB5730 6901 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2045 3421 2045
Size: 1376 5525
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3621 4711 278
Size: 53 83 186 936 2085 1090 2468
Fragments arranged by size:
2468 2085 1090 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4244 1937
Size: 2307 4594
Acc65I G'GTAC_C
Cuts at: 4222 4222
Size: 6901
AclI AA'CG_TT
Cuts at: 1563 5028 5401 1563
Size: 3465 373 3063
Fragments arranged by size:
3465 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2299 2608 3432 3482 3857 4964 6348 6616
Size: 667 309 824 50 375 1107 1384 268
Cuts at: 6616 1632
Size: 1917
Fragments arranged by size:
1917 1384 1107 824 667 375 309 268
50
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6704
AflIII A'CryG_T
Cuts at: 1203 3039 5800 6777 1203
Size: 1836 2761 977 1327
Fragments arranged by size:
2761 1836 1327 977
AgeI A'CCGG_T
Cuts at: 5732 5732
Size: 6901
AhdI GACnn_n'nnGTC
Cuts at: 2953 5630 2953
Size: 2677 4224
AleI CACnn'nnGTG
Cuts at: 1166 2291 1166
Size: 1125 5776
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6901
AlwNI CAG_nnn'CTG
Cuts at: 2432 2501 2860 3178 4188 4644 6216 2432
Size: 69 359 318 1010 456 1572 3117
Fragments arranged by size:
3117 1572 1010 456 359 318 69
ApaI G_GGCC'C
Cuts at: 3460 4116 3460
Size: 656 6245
ApaLI G'TGCA_C
Cuts at: 4155 4958 6114 4155
Size: 803 1156 4942
Fragments arranged by size:
4942 1156 803
ApoI r'AATT_y
Cuts at: 1493 2413 4210 4436 4588 6697 1493
Size: 920 1797 226 152 2109 1697
Fragments arranged by size:
2109 1797 1697 920 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5455 160
Size: 872 4423 1606
Fragments arranged by size:
4423 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6901
AvaI C'yCGr_G
Cuts at: 1870 1943 3301 4226 1870
Size: 73 1358 925 4545
Fragments arranged by size:
4545 1358 925 73
AvrII C'CTAG_G
Cuts at: 2635 2635
Size: 6901
BamHI G'GATC_C
Cuts at: 4231 4598 4231
Size: 367 6534
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2421 3969 4222 5677 618
Size: 325 217 627 634 1548 253 1455 1842
Fragments arranged by size:
1842 1548 1455 634 627 325 253 217
BanII G_rGCy'C
Cuts at: 1391 2996 3264 3460 4116 4220 1391
Size: 1605 268 196 656 104 4072
Fragments arranged by size:
4072 1605 656 268 196 104
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5930
BbvCI CC'TCA_GC
Cuts at: 4004 4118 4004
Size: 114 6787
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3238 3272 3499 3533 5092 5126 1903
Size: 34 1301 34 227 34 1559 34 3678
Fragments arranged by size:
3678 1559 1301 227 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2467 4793 6003 2467
Size: 2326 1210 3365
Fragments arranged by size:
3365 2326 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6901
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5512 136
Size: 107 122 71 631 605 3840 1525
Fragments arranged by size:
3840 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2727 2739 4017 6896 2727
Size: 12 1278 2879 2732
Fragments arranged by size:
2879 2732 1278 12
BlpI GC'TnA_GC
Cuts at: 3167 4318 3167
Size: 1151 5750
Bme1580I G_kGCm'C
Cuts at: 1790 2887 3460 4116 4159 4962 6118 1790
Size: 1097 573 656 43 803 1156 2573
Fragments arranged by size:
2573 1156 1097 803 656 573 43
BmgBI CAC'GTC
Cuts at: 1318 3022 1318
Size: 1704 5197
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4632 5590 442
Size: 416 53 83 399 24 3215 958 1753
Fragments arranged by size:
3215 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3932 1056
Size: 2876 4025
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3015 3015
Size: 6901
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2076 2095 2423 2891 3111 3800 4165 5561
Size: 837 19 328 468 220 689 365 1396
Cuts at: 5561 1239
Size: 2579
Fragments arranged by size:
2579 1396 837 689 468 365 328 220
19
Bpu10I CC'TnA_GC
Cuts at: 2990 3530 4004 4118 2990
Size: 540 474 114 5773
Fragments arranged by size:
5773 540 474 114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2690 2909 5026 5891 6189 2690
Size: 219 2117 865 298 3402
Fragments arranged by size:
3402 2117 865 298 219
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2336 2787 3386 5564 882
Size: 617 837 451 599 2178 2219
Fragments arranged by size:
2219 2178 837 617 599 451
BsaAI yAC'GTr
Cuts at: 493 1204 4160 4692 493
Size: 711 2956 532 2702
Fragments arranged by size:
2956 2702 711 532
BsaBI GATnn'nnATC
Cuts at: 6895 6895
Size: 6901
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3618 4708 5090 5725
Size: 53 83 186 936 2085 1090 382 635
Cuts at: 5725 275
Size: 1451
Fragments arranged by size:
2085 1451 1090 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3540 5334 5732 6006 6153 6564 3540
Size: 1794 398 274 147 411 3877
Fragments arranged by size:
3877 1794 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3109 3139 1178
Size: 30 165 30 1706 30 4940
Fragments arranged by size:
4940 1706 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2453 2540 2805 3071 3254 3404 3662 1186
Size: 1267 87 265 266 183 150 258 4425
Fragments arranged by size:
4425 1267 266 265 258 183 150 87
BseYI C'CCAG_C
Cuts at: 2773 2785 3992 4011 4058 4129 6104 6600 2773
Size: 12 1207 19 47 71 1975 496 3074
Fragments arranged by size:
3074 1975 1207 496 71 47 19 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2287 1163
Size: 573 551 5777
Fragments arranged by size:
5777 573 551
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4351 5112 6140 1540
Size: 275 186 2350 761 1028 2301
Fragments arranged by size:
2350 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2090 3308 4159 4168 4962 5047 6118 2090
Size: 1218 851 9 794 85 1071 2873
Fragments arranged by size:
2873 1218 1071 851 794 85 9
BsmI GAATG_Cn'
Cuts at: 3913 4412 4505 3913
Size: 499 93 6309
Fragments arranged by size:
6309 499 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 2887 2996 3264 3308 3460 4116
Size: 399 300 797 109 268 44 152 656
Cuts at: 4116 4159 4168 4220 4962 5047 6118 1391
Size: 43 9 52 742 85 1071 2174
Fragments arranged by size:
2174 1071 797 742 656 399 300 268
152 109 85 52 44 43 9
BspEI T'CCGG_A
Cuts at: 6564 6564
Size: 6901
BspHI T'CATG_A
Cuts at: 4790 6520 4790
Size: 1730 5171
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2045 3421 4256 844
Size: 1201 1376 835 3489
Fragments arranged by size:
3489 1376 1201 835
BsrBI CCG'CTC
Cuts at: 1394 1823 4788 1394
Size: 429 2965 3507
Fragments arranged by size:
3507 2965 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5396 5570 65
Size: 1183 4148 174 1396
Fragments arranged by size:
4148 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5545 5732 1700
Size: 110 3735 187 2869
Fragments arranged by size:
3735 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6901
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6901
BssSI C'ACGA_G
Cuts at: 3008 3746 4961 5973 3008
Size: 738 1215 1012 3936
Fragments arranged by size:
3936 1215 1012 738
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4163 794
Size: 3369 3532
BstBI TT'CG_AA
Cuts at: 4027 5705 4027
Size: 1678 5223
BstEII G'GTnAC_C
Cuts at: 1184 3115 1184
Size: 1931 4970
BstXI CCAn_nnnn'nTGG
Cuts at: 2057 2219 2057
Size: 162 6739
BstYI r'GATC_y
Cuts at: 1972 2254 2727 2739 4017 4231 4598 4983 5000
Size: 282 473 12 1278 214 367 385 17
Cuts at: 5000 6452 6538 6550 6896 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1278 473 385 367 346 282
214 86 17 12 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2109 2126 3973 4108 513
Size: 552 939 105 17 1847 135 3306
Fragments arranged by size:
3306 1847 939 552 135 105 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3919 487
Size: 683 56 2693 3469
Fragments arranged by size:
3469 2693 683 56
BtsI GCAGTG_nn'
Cuts at: 2139 3333 4417 5211 5231 2139
Size: 1194 1084 794 20 3809
Fragments arranged by size:
3809 1194 1084 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6901
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3578 3613 529
Size: 35 3014 35 3817
Fragments arranged by size:
3817 3014 35 35
DraI TTT'AAA
Cuts at: 4205 4205
Size: 6901
DraIII CAC_nnn'GTG
Cuts at: 4163 4163
Size: 6901
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5908 809
Size: 507 4592 1802
Fragments arranged by size:
4592 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2136 2608 4348
Size: 54 1425 50 275 18 306 472 1740
Cuts at: 4348 5237 6866 8
Size: 889 1629 43
Fragments arranged by size:
1740 1629 1425 889 472 306 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4348 1537
Size: 275 2536 4090
Fragments arranged by size:
4090 2536 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2025 2091 2824 2903 4831 1353
Size: 672 66 733 79 1928 3423
Fragments arranged by size:
3423 1928 733 672 79 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5488 5862 6008 387
Size: 1064 138 3899 374 146 1280
Fragments arranged by size:
3899 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2076 2095 2299 2423 2608 2891 3111
Size: 393 444 19 204 124 185 283 220
Cuts at: 3111 3432 3482 3800 3857 4165 4964 5561 6348
Size: 321 50 318 57 308 799 597 787
Cuts at: 6348 6616 1239
Size: 268 1524
Fragments arranged by size:
1524 799 787 597 444 393 321 318
308 283 268 220 204 185 124 57
50 19
EcoNI CCTnn'n_nnAGG
Cuts at: 2329 4138 2329
Size: 1809 5092
EcoO109I rG'GnC_Cy
Cuts at: 2639 2865 3457 4113 2639
Size: 226 592 656 5427
Fragments arranged by size:
5427 656 592 226
EcoRI G'AATT_C
Cuts at: 2413 4210 4588 2413
Size: 1797 378 4726
Fragments arranged by size:
4726 1797 378
FspI TGC'GCA
Cuts at: 5407 5407
Size: 6901
HaeII r_GCGC'y
Cuts at: 1678 3726 3844 6048 1678
Size: 2048 118 2204 2531
Fragments arranged by size:
2531 2204 2048 118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2939 2971 3109 3141 5542
Size: 32 135 32 1367 32 138 32 2401
Cuts at: 5542 5574 5616 5648 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2401 1367 138 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4245 4491 669
Size: 988 281 2307 246 3079
Fragments arranged by size:
3079 2307 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6901
HpaI GTT'AAC
Cuts at: 4491 4491
Size: 6901
KpnI G_GTAC'C
Cuts at: 4226 4226
Size: 6901
MfeI C'AATT_G
Cuts at: 4500 4500
Size: 6901
MluI A'CGCG_T
Cuts at: 6777 6777
Size: 6901
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3700 6015 6199 1305
Size: 2395 2315 184 2007
Fragments arranged by size:
2395 2315 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2138 2610 10
Size: 54 1425 343 306 472 4301
Fragments arranged by size:
4301 1425 472 343 306 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2245 2291 2981 4859 5218 5377 518
Size: 648 1079 46 690 1878 359 159 2042
Fragments arranged by size:
2042 1878 1079 690 648 359 159 46
MspA1I CmG'CkG
Cuts at: 1606 2773 2878 3665 4152 4188 4317 4994 6142
Size: 1167 105 787 487 36 129 677 1148
Cuts at: 6142 6387 1606
Size: 245 2120
Fragments arranged by size:
2120 1167 1148 787 677 487 245 129
105 36
NcoI C'CATG_G
Cuts at: 513 1065 2004 2126 3973 513
Size: 552 939 122 1847 3441
Fragments arranged by size:
3441 1847 939 552 122
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6901
NheI G'CTAG_C
Cuts at: 1052 3928 1052
Size: 2876 4025
NotI GC'GGCC_GC
Cuts at: 4348 4348
Size: 6901
NspI r_CATG'y
Cuts at: 1132 1229 3043 4259 5804 1132
Size: 97 1814 1216 1545 2229
Fragments arranged by size:
2229 1814 1545 1216 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6901
PciI A'CATG_T
Cuts at: 3039 5800 3039
Size: 2761 4140
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2432 2949 3022 3403 1108
Size: 1324 517 73 381 4606
Fragments arranged by size:
4606 1324 517 381 73
PfoI T'CCnGG_A
Cuts at: 2259 3147 3342 3816 2259
Size: 888 195 474 5344
Fragments arranged by size:
5344 888 474 195
PmeI GTTT'AAAC
Cuts at: 4205 4205
Size: 6901
PmlI CAC'GTG
Cuts at: 4160 4160
Size: 6901
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4970 5002 6483 6515 4970
Size: 32 1481 32 5356
Fragments arranged by size:
5356 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2639 2639
Size: 6901
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6901
PsiI TTA'TAA
Cuts at: 4471 4471
Size: 6901
PspOMI G'GGCC_C
Cuts at: 3456 4112 3456
Size: 656 6245
PstI C_TGCA'G
Cuts at: 830 2497 3090 3432 3522 3916 3945 4253 830
Size: 1667 593 342 90 394 29 308 3478
Fragments arranged by size:
3478 1667 593 394 342 308 90 29
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6901
PvuII CAG'CTG
Cuts at: 2773 3665 2773
Size: 892 6009
SalI G'TCGA_C
Cuts at: 1936 4243 1936
Size: 2307 4594
SapI GCTCTTCn'nnn_
Cuts at: 2025 2903 2025
Size: 878 6023
SbfI CC_TGCA'GG
Cuts at: 4253 4253
Size: 6901
ScaI AGT'ACT
Cuts at: 5149 6861 5149
Size: 1712 5189
SfcI C'TryA_G
Cuts at: 826 1045 2493 3086 3428 3518 3912 3941 4249
Size: 219 1448 593 342 90 394 29 308
Cuts at: 4249 5384 6065 6256 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1448 1135 681 593 394 342 308
219 191 90 29
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6901
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6901
SmaI CCC'GGG
Cuts at: 1872 4228 1872
Size: 2356 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2705 2924 5005 5906 6168 820
Size: 197 926 762 219 2081 901 262 1553
Fragments arranged by size:
2081 1553 926 901 762 262 219 197
SnaBI TAC'GTA
Cuts at: 493 4692 493
Size: 4199 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6901
SphI G_CATG'C
Cuts at: 1132 4259 1132
Size: 3127 3774
SspI AAT'ATT
Cuts at: 5 52 3029 4825 5
Size: 47 2977 1796 2081
Fragments arranged by size:
2977 2081 1796 47
StuI AGG'CCT
Cuts at: 1517 3293 1517
Size: 1776 5125
StyI C'CwwG_G
Cuts at: 513 1065 2004 2126 2635 3611 3973 4340 513
Size: 552 939 122 509 976 362 367 3074
Fragments arranged by size:
3074 976 939 552 509 367 362 122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2439 3217 4928 4945 5098 5283 5728
Size: 1255 859 778 1711 17 153 185 445
Cuts at: 5728 325
Size: 1498
Fragments arranged by size:
1711 1498 1255 859 778 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5147 6859 96
Size: 274 80 33 51 1443 3170 1712 138
Fragments arranged by size:
3170 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2275 2307 2655 2687 2835 2867 6861 6893 2275
Size: 32 348 32 148 32 3994 32 2283
Fragments arranged by size:
3994 2283 348 148 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6901
XbaI T'CTAG_A
Cuts at: 4237 4237
Size: 6901
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6901
XmaI C'CCGG_G
Cuts at: 1870 4226 1870
Size: 2356 4545
XmnI GAAnn'nnTTC
Cuts at: 2447 3329 5030 2447
Size: 882 1701 4318
Fragments arranged by size:
4318 1701 882
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3619 4709 276
Size: 53 83 186 936 2085 1090 2468
Fragments arranged by size:
2468 2085 1090 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI