Restriction map for
pFN21AB5728
(Circular) (Six-base) MAPSORT of: pFN21AB5728.seq Check: 5758 from: 1 to: 6898
pFN21AB5728 6898 bp
With 182 enzymes: SgfI *
March 28, 2012 12:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4708 278
Size: 53 83 186 936 3172 2468
Fragments arranged by size:
3172 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3629 4241 1937
Size: 1692 612 4594
Fragments arranged by size:
4594 1692 612
Acc65I G'GTAC_C
Cuts at: 4219 4219
Size: 6898
AclI AA'CG_TT
Cuts at: 1563 5025 5398 1563
Size: 3462 373 3063
Fragments arranged by size:
3462 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3579 4961 6345 6613 1632
Size: 1947 1382 1384 268 1917
Fragments arranged by size:
1947 1917 1384 1382 268
AfeI AGC'GCT
Cuts at: 2142 2142
Size: 6898
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6701
AflIII A'CryG_T
Cuts at: 1203 5797 6774 1203
Size: 4594 977 1327
Fragments arranged by size:
4594 1327 977
AgeI A'CCGG_T
Cuts at: 5729 5729
Size: 6898
AhdI GACnn_n'nnGTC
Cuts at: 5627 5627
Size: 6898
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6898
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6898
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2600 2632 2600
Size: 32 6866
AlwNI CAG_nnn'CTG
Cuts at: 3602 4641 6213 3602
Size: 1039 1572 4287
Fragments arranged by size:
4287 1572 1039
ApaI G_GGCC'C
Cuts at: 2195 2195
Size: 6898
ApaLI G'TGCA_C
Cuts at: 4955 6111 4955
Size: 1156 5742
ApoI r'AATT_y
Cuts at: 1493 2801 3176 3287 3689 4097 4207 4433 4585
Size: 1308 375 111 402 408 110 226 152
Cuts at: 4585 6694 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1308 408 402 375 226 152
111 110
AseI AT'TA_AT
Cuts at: 160 1032 5452 160
Size: 872 4420 1606
Fragments arranged by size:
4420 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6898
AvaI C'yCGr_G
Cuts at: 1870 1943 2051 2176 2186 4223 1870
Size: 73 108 125 10 2037 4545
Fragments arranged by size:
4545 2037 125 108 73 10
AvrII C'CTAG_G
Cuts at: 2320 2320
Size: 6898
BamHI G'GATC_C
Cuts at: 3577 4228 4595 3577
Size: 651 367 5880
Fragments arranged by size:
5880 651 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2215 4219 5674 618
Size: 325 217 627 428 2004 1455 1842
Fragments arranged by size:
2004 1842 1455 627 428 325 217
BanII G_rGCy'C
Cuts at: 1391 2036 2195 2373 4217 1391
Size: 645 159 178 1844 4072
Fragments arranged by size:
4072 1844 645 178 159
BbeI G_GCGC'C
Cuts at: 2219 2219
Size: 6898
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3105 928
Size: 971 1206 4721
Fragments arranged by size:
4721 1206 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2386 2420 5089 5123 1903
Size: 34 449 34 2669 34 3678
Fragments arranged by size:
3678 2669 449 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2565 4790 6000 2565
Size: 2225 1210 3463
Fragments arranged by size:
3463 2225 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6898
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2190 5509 136
Size: 107 122 71 631 605 518 3319 1525
Fragments arranged by size:
3319 1525 631 605 518 122 107 71
BglII A'GATC_T
Cuts at: 6893 6893
Size: 6898
BlpI GC'TnA_GC
Cuts at: 4315 4315
Size: 6898
Bme1580I G_kGCm'C
Cuts at: 1790 2195 4126 4959 6115 1790
Size: 405 1931 833 1156 2573
Fragments arranged by size:
2573 1931 1156 833 405
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6898
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4629 5587 442
Size: 416 53 83 399 24 3212 958 1753
Fragments arranged by size:
3212 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3468 1056
Size: 2412 4486
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2299 2991 3342 4138 5558 1239
Size: 1060 692 351 796 1420 2579
Fragments arranged by size:
2579 1420 1060 796 692 351
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2792 5023 5888 6186 2792
Size: 2231 865 298 3504
Fragments arranged by size:
3504 2231 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5561 882
Size: 617 4062 2219
Fragments arranged by size:
4062 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4689 493
Size: 711 3485 2702
Fragments arranged by size:
3485 2702 711
BsaBI GATnn'nnATC
Cuts at: 6892 6892
Size: 6898
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2216 4705 5087 5722
Size: 53 83 186 936 683 2489 382 635
Cuts at: 5722 275
Size: 1451
Fragments arranged by size:
2489 1451 936 683 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5331 5729 6003 6150 6561 5331
Size: 398 274 147 411 5668
Fragments arranged by size:
5668 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6673
Fragments arranged by size:
6673 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2059 2077 2339 1186
Size: 873 18 262 5745
Fragments arranged by size:
5745 873 262 18
BseYI C'CCAG_C
Cuts at: 2039 2288 6101 6597 2039
Size: 249 3813 496 2340
Fragments arranged by size:
3813 2340 496 249
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2762 3750 1163
Size: 573 1026 988 4311
Fragments arranged by size:
4311 1026 988 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4348 5109 6137 1540
Size: 275 186 2347 761 1028 2301
Fragments arranged by size:
2347 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4959 5044 6115 4959
Size: 85 1071 5742
Fragments arranged by size:
5742 1071 85
BsmI GAATG_Cn'
Cuts at: 3191 4409 4502 3191
Size: 1218 93 5587
Fragments arranged by size:
5587 1218 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2195 2373 4126 4217 4959 5044
Size: 399 246 159 178 1753 91 742 85
Cuts at: 5044 6115 1391
Size: 1071 2174
Fragments arranged by size:
2174 1753 1071 742 399 246 178 159
91 85
BspEI T'CCGG_A
Cuts at: 6561 6561
Size: 6898
BspHI T'CATG_A
Cuts at: 4787 6517 4787
Size: 1730 5168
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3416 4253 844
Size: 2572 837 3489
Fragments arranged by size:
3489 2572 837
BsrBI CCG'CTC
Cuts at: 1394 1823 4785 1394
Size: 429 2962 3507
Fragments arranged by size:
3507 2962 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5393 5567 65
Size: 1183 4145 174 1396
Fragments arranged by size:
4145 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2171 2294 5542 5729 1700
Size: 110 361 123 3248 187 2869
Fragments arranged by size:
3248 2869 361 187 123 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6898
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6898
BssSI C'ACGA_G
Cuts at: 4958 5970 4958
Size: 1012 5886
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6898
BstBI TT'CG_AA
Cuts at: 3416 5702 3416
Size: 2286 4612
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6898
BstYI r'GATC_y
Cuts at: 1972 2847 3577 4228 4595 4980 4997 6449 6535
Size: 875 730 651 367 385 17 1452 86
Cuts at: 6535 6547 6893 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 875 730 651 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2121 2253 513
Size: 552 1056 132 5158
Fragments arranged by size:
5158 1056 552 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6159
Fragments arranged by size:
6159 683 56
BtsI GCAGTG_nn'
Cuts at: 3116 3381 3475 4414 5208 5228 3116
Size: 265 94 939 794 20 4786
Fragments arranged by size:
4786 939 794 265 94 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6898
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2450 2485 529
Size: 35 1886 35 4942
Fragments arranged by size:
4942 1886 35 35
DraI TTT'AAA
Cuts at: 4202 4202
Size: 6898
DraIII CAC_nnn'GTG
Cuts at: 2635 2635
Size: 6898
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5905 809
Size: 507 4589 1802
Fragments arranged by size:
4589 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2280 2458 2650
Size: 54 1425 50 275 18 450 178 192
Cuts at: 2650 2709 4345 5234 6863 8
Size: 59 1636 889 1629 43
Fragments arranged by size:
1636 1629 1425 889 450 275 192 178
59 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4345 1537
Size: 275 2533 4090
Fragments arranged by size:
4090 2533 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2107 2868 3539 3706 4828 1353
Size: 754 761 671 167 1122 3423
Fragments arranged by size:
3423 1122 761 754 671 167
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2071 5485 5859 6005 387
Size: 1064 138 482 3414 374 146 1280
Fragments arranged by size:
3414 1280 1064 482 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2299 2991 3342 3579 4138 4961 5558
Size: 393 667 692 351 237 559 823 597
Cuts at: 5558 6345 6613 1239
Size: 787 268 1524
Fragments arranged by size:
1524 823 787 692 667 597 559 393
351 268 237
EcoO109I rG'GnC_Cy
Cuts at: 2055 2191 2055
Size: 136 6762
EcoRI G'AATT_C
Cuts at: 2801 4207 4585 2801
Size: 1406 378 5114
Fragments arranged by size:
5114 1406 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2717 2717
Size: 6898
FspI TGC'GCA
Cuts at: 5404 5404
Size: 6898
HaeII r_GCGC'y
Cuts at: 1678 2144 2219 2295 6045 1678
Size: 466 75 76 3750 2531
Fragments arranged by size:
3750 2531 466 76 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2334 2366 4124 4156 5539
Size: 32 135 32 762 32 1758 32 1383
Cuts at: 5539 5571 5613 5645 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1758 1383 762 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3562 4242 4488 669
Size: 988 281 1624 680 246 3079
Fragments arranged by size:
3079 1624 988 680 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6898
HpaI GTT'AAC
Cuts at: 4488 4488
Size: 6898
KasI G'GCGC_C
Cuts at: 2215 2215
Size: 6898
KpnI G_GTAC'C
Cuts at: 4223 4223
Size: 6898
MfeI C'AATT_G
Cuts at: 4497 4497
Size: 6898
MluI A'CGCG_T
Cuts at: 6774 6774
Size: 6898
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2709 3150 3416 3533 6012 6196 1305
Size: 1404 441 266 117 2479 184 2007
Fragments arranged by size:
2479 2007 1404 441 266 184 117
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2460 2652 2711 10
Size: 54 1425 343 628 192 59 4197
Fragments arranged by size:
4197 1425 628 343 192 59 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3750 3924 4856 5215 5374 518
Size: 648 2584 174 932 359 159 2042
Fragments arranged by size:
2584 2042 932 648 359 174 159
MspA1I CmG'CkG
Cuts at: 1606 2039 2274 2328 3426 4314 4991 6139 6384
Size: 433 235 54 1098 888 677 1148 245
Cuts at: 6384 1606
Size: 2120
Fragments arranged by size:
2120 1148 1098 888 677 433 245 235
54
NaeI GCC'GGC
Cuts at: 2173 2296 2173
Size: 123 6775
NarI GG'CG_CC
Cuts at: 2216 2216
Size: 6898
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6346
NdeI CA'TA_TG
Cuts at: 387 3241 387
Size: 2854 4044
NgoMIV G'CCGG_C
Cuts at: 2171 2294 2171
Size: 123 6775
NheI G'CTAG_C
Cuts at: 1052 3464 1052
Size: 2412 4486
NotI GC'GGCC_GC
Cuts at: 4345 4345
Size: 6898
NspI r_CATG'y
Cuts at: 1132 1229 2777 4256 5801 1132
Size: 97 1548 1479 1545 2229
Fragments arranged by size:
2229 1548 1545 1479 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6898
PciI A'CATG_T
Cuts at: 5797 5797
Size: 6898
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6898
PfoI T'CCnGG_A
Cuts at: 3366 3366
Size: 6898
PmeI GTTT'AAAC
Cuts at: 4202 4202
Size: 6898
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4967 4999 6480 6512 4967
Size: 32 1481 32 5353
Fragments arranged by size:
5353 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2055 2055
Size: 6898
PshAI GACnn'nnGTC
Cuts at: 1613 3517 1613
Size: 1904 4994
PsiI TTA'TAA
Cuts at: 4468 4468
Size: 6898
PspOMI G'GGCC_C
Cuts at: 2191 2191
Size: 6898
PstI C_TGCA'G
Cuts at: 830 4250 830
Size: 3420 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6898
PvuII CAG'CTG
Cuts at: 2274 3426 2274
Size: 1152 5746
SalI G'TCGA_C
Cuts at: 1936 4240 1936
Size: 2304 4594
SbfI CC_TGCA'GG
Cuts at: 4250 4250
Size: 6898
ScaI AGT'ACT
Cuts at: 5146 6858 5146
Size: 1712 5186
SfcI C'TryA_G
Cuts at: 826 1045 2786 4246 5381 6062 6253 826
Size: 219 1741 1460 1135 681 191 1471
Fragments arranged by size:
1741 1471 1460 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2190 2190
Size: 6898
SfoI GGC'GCC
Cuts at: 2217 2217
Size: 6898
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6898
SgrAI Cr'CCGG_yG
Cuts at: 1700 2294 1700
Size: 594 6304
SmaI CCC'GGG
Cuts at: 1872 2053 4225 1872
Size: 181 2172 4545
Fragments arranged by size:
4545 2172 181
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2807 5002 5903 6165 820
Size: 197 926 864 2195 901 262 1553
Fragments arranged by size:
2195 1553 926 901 864 262 197
SnaBI TAC'GTA
Cuts at: 493 4689 493
Size: 4196 2702
SpeI A'CTAG_T
Cuts at: 152 2737 152
Size: 2585 4313
SphI G_CATG'C
Cuts at: 1132 4256 1132
Size: 3124 3774
SspI AAT'ATT
Cuts at: 5 52 2542 4822 5
Size: 47 2490 2280 2081
Fragments arranged by size:
2490 2280 2081 47
StuI AGG'CCT
Cuts at: 1517 3945 1517
Size: 2428 4470
StyI C'CwwG_G
Cuts at: 513 1065 2320 3044 4337 513
Size: 552 1255 724 1293 3074
Fragments arranged by size:
3074 1293 1255 724 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4089 4925 4942 5095 5280 5725 325
Size: 1255 2509 836 17 153 185 445 1498
Fragments arranged by size:
2509 1498 1255 836 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4002 5144 6856
Size: 274 80 33 51 1443 2025 1142 1712
Cuts at: 6856 96
Size: 138
Fragments arranged by size:
2025 1712 1443 1142 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6858 6890 6858
Size: 32 6866
Tth111I GACn'n_nGTC
Cuts at: 1316 3359 1316
Size: 2043 4855
XbaI T'CTAG_A
Cuts at: 3637 4234 3637
Size: 597 6301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3712 3712
Size: 6898
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6898
XmaI C'CCGG_G
Cuts at: 1870 2051 4223 1870
Size: 181 2172 4545
Fragments arranged by size:
4545 2172 181
XmnI GAAnn'nnTTC
Cuts at: 2580 5027 2580
Size: 2447 4451
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4706 276
Size: 53 83 186 936 3172 2468
Fragments arranged by size:
3172 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI
FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SrfI SwaI