Restriction map for
pFN21AB5724
(Circular) (Six-base) MAPSORT of: pFN21AB5724.seq Check: 3175 from: 1 to: 6892
pFN21AB5724 6892 bp
With 182 enzymes: SgfI *
August 16, 2010 14:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4702 278
Size: 53 83 186 936 3166 2468
Fragments arranged by size:
3166 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4235 1937
Size: 2298 4594
Acc65I G'GTAC_C
Cuts at: 4213 4213
Size: 6892
AclI AA'CG_TT
Cuts at: 1563 4179 5019 5392 1563
Size: 2616 840 373 3063
Fragments arranged by size:
3063 2616 840 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2807 3545 4955 6339 6607 1632
Size: 1175 738 1410 1384 268 1917
Fragments arranged by size:
1917 1410 1384 1175 738 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6695
AflIII A'CryG_T
Cuts at: 1203 5791 6768 1203
Size: 4588 977 1327
Fragments arranged by size:
4588 1327 977
AgeI A'CCGG_T
Cuts at: 5723 5723
Size: 6892
AhdI GACnn_n'nnGTC
Cuts at: 3080 5621 3080
Size: 2541 4351
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6892
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6892
AlwNI CAG_nnn'CTG
Cuts at: 2333 2651 4635 6207 2333
Size: 318 1984 1572 3018
Fragments arranged by size:
3018 1984 1572 318
ApaLI G'TGCA_C
Cuts at: 2399 4949 6105 2399
Size: 2550 1156 3186
Fragments arranged by size:
3186 2550 1156
ApoI r'AATT_y
Cuts at: 1493 2168 2264 2336 3192 3495 3755 4201 4427
Size: 675 96 72 856 303 260 446 226
Cuts at: 4427 4579 6688 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 856 675 446 303 260 226
152 96 72
AseI AT'TA_AT
Cuts at: 160 1032 3179 3317 3350 5446 160
Size: 872 2147 138 33 2096 1606
Fragments arranged by size:
2147 2096 1606 872 138 33
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6892
AvaI C'yCGr_G
Cuts at: 1870 1943 2814 4217 1870
Size: 73 871 1403 4545
Fragments arranged by size:
4545 1403 871 73
BamHI G'GATC_C
Cuts at: 4222 4589 4222
Size: 367 6525
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4213 5668 618
Size: 325 217 627 2426 1455 1842
Fragments arranged by size:
2426 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4211 1391
Size: 2820 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2089 2813 3551 928
Size: 971 190 724 738 4269
Fragments arranged by size:
4269 971 738 724 190
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5083 5117 1903
Size: 34 50 34 3062 34 3678
Fragments arranged by size:
3678 3062 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3324 4784 5994 3324
Size: 1460 1210 4222
Fragments arranged by size:
4222 1460 1210
BclI T'GATC_A
Cuts at: 1548 3399 1548
Size: 1851 5041
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5503 136
Size: 107 122 71 631 605 3831 1525
Fragments arranged by size:
3831 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6887 6887
Size: 6892
BlpI GC'TnA_GC
Cuts at: 4309 4309
Size: 6892
Bme1580I G_kGCm'C
Cuts at: 1790 2403 3616 4953 6109 1790
Size: 613 1213 1337 1156 2573
Fragments arranged by size:
2573 1337 1213 1156 613
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6892
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4623 5581 442
Size: 416 53 83 399 24 3206 958 1753
Fragments arranged by size:
3206 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3243 1056
Size: 2187 4705
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2766 2800 3570 5552 1239
Size: 1527 34 770 1982 2579
Fragments arranged by size:
2579 1982 1527 770 34
Bpu10I CC'TnA_GC
Cuts at: 3249 3249
Size: 6892
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5017 5882 6180 5017
Size: 865 298 5729
Fragments arranged by size:
5729 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5555 882
Size: 617 4056 2219
Fragments arranged by size:
4056 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4683 493
Size: 711 3479 2702
Fragments arranged by size:
3479 2702 711
BsaBI GATnn'nnATC
Cuts at: 3464 6886 3464
Size: 3422 3470
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4699 5081 5716 275
Size: 53 83 186 936 3166 382 635 1451
Fragments arranged by size:
3166 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5325 5723 5997 6144 6555 5325
Size: 398 274 147 411 5662
Fragments arranged by size:
5662 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2592 2622 1178
Size: 30 165 30 1189 30 5448
Fragments arranged by size:
5448 1189 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2243 1186
Size: 1057 5835
BseYI C'CCAG_C
Cuts at: 6095 6591 6095
Size: 496 6396
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2502 1163
Size: 573 766 5553
Fragments arranged by size:
5553 766 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4342 5103 6131 1540
Size: 275 186 2341 761 1028 2301
Fragments arranged by size:
2341 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2015 2403 4953 5038 6109 2015
Size: 388 2550 85 1071 2798
Fragments arranged by size:
2798 2550 1071 388 85
BsmI GAATG_Cn'
Cuts at: 4403 4496 4403
Size: 93 6799
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2403 3616 4211 4953 5038 6109
Size: 399 225 388 1213 595 742 85 1071
Cuts at: 6109 1391
Size: 2174
Fragments arranged by size:
2174 1213 1071 742 595 399 388 225
85
BspEI T'CCGG_A
Cuts at: 6555 6555
Size: 6892
BspHI T'CATG_A
Cuts at: 3010 4781 6511 3010
Size: 1771 1730 3391
Fragments arranged by size:
3391 1771 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4247 844
Size: 3403 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4779 1394
Size: 429 2956 3507
Fragments arranged by size:
3507 2956 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5387 5561 65
Size: 1183 4139 174 1396
Fragments arranged by size:
4139 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5536 5723 1700
Size: 110 3726 187 2869
Fragments arranged by size:
3726 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2460 96
Size: 2364 4528
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6892
BssSI C'ACGA_G
Cuts at: 4952 5964 4952
Size: 1012 5880
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6892
BstBI TT'CG_AA
Cuts at: 5696 5696
Size: 6892
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6892
BstXI CCAn_nnnn'nTGG
Cuts at: 2778 2778
Size: 6892
BstYI r'GATC_y
Cuts at: 1972 4222 4589 4974 4991 6443 6529 6541 6887
Size: 2250 367 385 17 1452 86 12 346
Cuts at: 6887 1972
Size: 1977
Fragments arranged by size:
2250 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 4158 513
Size: 552 3093 3247
Fragments arranged by size:
3247 3093 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6153
Fragments arranged by size:
6153 683 56
BtsI GCAGTG_nn'
Cuts at: 2574 2657 4408 5202 5222 2574
Size: 83 1751 794 20 4244
Fragments arranged by size:
4244 1751 794 83 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6892
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6857
DraI TTT'AAA
Cuts at: 4196 4196
Size: 6892
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5899 809
Size: 507 4583 1802
Fragments arranged by size:
4583 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4339 5228 6857
Size: 54 1425 50 275 18 2509 889 1629
Cuts at: 6857 8
Size: 43
Fragments arranged by size:
2509 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4339 1537
Size: 275 2527 4090
Fragments arranged by size:
4090 2527 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2220 4083 4822 1353
Size: 867 1863 739 3423
Fragments arranged by size:
3423 1863 867 739
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5479 5853 5999 387
Size: 1064 138 3890 374 146 1280
Fragments arranged by size:
3890 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2766 2800 2807 3545 3570 4955 5552
Size: 393 1134 34 7 738 25 1385 597
Cuts at: 5552 6339 6607 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1385 1134 787 738 597 393 268
34 25 7
EcoO109I rG'GnC_Cy
Cuts at: 2681 2681
Size: 6892
EcoRI G'AATT_C
Cuts at: 2336 4201 4579 2336
Size: 1865 378 4649
Fragments arranged by size:
4649 1865 378
EcoRV GAT'ATC
Cuts at: 2410 3568 2410
Size: 1158 5734
FspI TGC'GCA
Cuts at: 5398 5398
Size: 6892
HaeII r_GCGC'y
Cuts at: 1678 6039 1678
Size: 4361 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2438 2470 3066 3098 3679
Size: 32 135 32 866 32 596 32 581
Cuts at: 3679 3711 3862 3894 5533 5565 5607 5639 1373
Size: 32 151 32 1639 32 42 32 2626
Fragments arranged by size:
2626 1639 866 596 581 151 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4183 4236 4482 669
Size: 988 281 2245 53 246 3079
Fragments arranged by size:
3079 2245 988 281 246 53
HindIII A'AGCT_T
Cuts at: 748 2134 748
Size: 1386 5506
HpaI GTT'AAC
Cuts at: 4482 4482
Size: 6892
KpnI G_GTAC'C
Cuts at: 4217 4217
Size: 6892
MfeI C'AATT_G
Cuts at: 2772 4491 2772
Size: 1719 5173
MluI A'CGCG_T
Cuts at: 6768 6768
Size: 6892
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6006 6190 1305
Size: 4701 184 2007
Fragments arranged by size:
4701 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5070
Fragments arranged by size:
5070 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4850 5209 5368 518
Size: 648 3684 359 159 2042
Fragments arranged by size:
3684 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4308 4985 6133 6378 1606
Size: 2702 677 1148 245 2120
Fragments arranged by size:
2702 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 4158 513
Size: 552 3093 3247
Fragments arranged by size:
3247 3093 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6892
NheI G'CTAG_C
Cuts at: 1052 3239 1052
Size: 2187 4705
NotI GC'GGCC_GC
Cuts at: 4339 4339
Size: 6892
NspI r_CATG'y
Cuts at: 1132 1229 2794 4250 5795 1132
Size: 97 1565 1456 1545 2229
Fragments arranged by size:
2229 1565 1545 1456 97
PacI TTA_AT'TAA
Cuts at: 3354 3354
Size: 6892
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6892
PciI A'CATG_T
Cuts at: 5791 5791
Size: 6892
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6892
PmeI GTTT'AAAC
Cuts at: 4196 4196
Size: 6892
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4961 4993 6474 6506 4961
Size: 32 1481 32 5347
Fragments arranged by size:
5347 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6892
PsiI TTA'TAA
Cuts at: 3735 4462 3735
Size: 727 6165
PstI C_TGCA'G
Cuts at: 830 3284 4244 830
Size: 2454 960 3478
Fragments arranged by size:
3478 2454 960
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6892
SalI G'TCGA_C
Cuts at: 1936 4234 1936
Size: 2298 4594
SbfI CC_TGCA'GG
Cuts at: 4244 4244
Size: 6892
ScaI AGT'ACT
Cuts at: 2253 5140 6852 2253
Size: 2887 1712 2293
Fragments arranged by size:
2887 2293 1712
SfcI C'TryA_G
Cuts at: 826 1045 3280 4240 5375 6056 6247 826
Size: 219 2235 960 1135 681 191 1471
Fragments arranged by size:
2235 1471 1135 960 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6892
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6892
SmaI CCC'GGG
Cuts at: 1872 4219 1872
Size: 2347 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4996 5897 6159 820
Size: 197 926 3053 901 262 1553
Fragments arranged by size:
3053 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4683 493
Size: 4190 2702
SpeI A'CTAG_T
Cuts at: 152 2909 152
Size: 2757 4135
SphI G_CATG'C
Cuts at: 1132 4250 1132
Size: 3118 3774
SspI AAT'ATT
Cuts at: 5 52 2203 2540 4816 5
Size: 47 2151 337 2276 2081
Fragments arranged by size:
2276 2151 2081 337 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6892
StyI C'CwwG_G
Cuts at: 513 1065 2053 4158 4331 513
Size: 552 988 2105 173 3074
Fragments arranged by size:
3074 2105 988 552 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2354 4919 4936 5089 5274 5719 325
Size: 1255 774 2565 17 153 185 445 1498
Fragments arranged by size:
2565 1498 1255 774 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2251 2460 2749
Size: 274 80 33 51 1443 274 209 289
Cuts at: 2749 3208 5138 6850 96
Size: 459 1930 1712 138
Fragments arranged by size:
1930 1712 1443 459 289 274 274 209
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2442 2474 3096 3128 6852 6884 2442
Size: 32 622 32 3724 32 2450
Fragments arranged by size:
3724 2450 622 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6892
XbaI T'CTAG_A
Cuts at: 4228 4228
Size: 6892
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2648 2648
Size: 6892
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6892
XmaI C'CCGG_G
Cuts at: 1870 4217 1870
Size: 2347 4545
XmnI GAAnn'nnTTC
Cuts at: 2728 3375 5021 2728
Size: 647 1646 4599
Fragments arranged by size:
4599 1646 647
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4700 276
Size: 53 83 186 936 3166 2468
Fragments arranged by size:
3166 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PacI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI