Restriction map for
pFN21AB5716
(Circular) (Six-base) MAPSORT of: pFN21AB5716.seq Check: 3757 from: 1 to: 6868
pFN21AB5716 6868 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2428 2428
Size: 6868
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4678 278
Size: 53 83 186 936 3142 2468
Fragments arranged by size:
3142 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3853 4211 1937
Size: 1916 358 4594
Fragments arranged by size:
4594 1916 358
Acc65I G'GTAC_C
Cuts at: 4189 4189
Size: 6868
AclI AA'CG_TT
Cuts at: 1563 4995 5368 1563
Size: 3432 373 3063
Fragments arranged by size:
3432 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2282 2561 3017 3404 3429 4931 6315 6583
Size: 650 279 456 387 25 1502 1384 268
Cuts at: 6583 1632
Size: 1917
Fragments arranged by size:
1917 1502 1384 650 456 387 279 268
25
AfeI AGC'GCT
Cuts at: 2030 2030
Size: 6868
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6671
AflIII A'CryG_T
Cuts at: 1203 4066 4107 5767 6744 1203
Size: 2863 41 1660 977 1327
Fragments arranged by size:
2863 1660 1327 977 41
AgeI A'CCGG_T
Cuts at: 2987 5699 2987
Size: 2712 4156
AhdI GACnn_n'nnGTC
Cuts at: 3510 5597 3510
Size: 2087 4781
AleI CACnn'nnGTG
Cuts at: 1166 3147 1166
Size: 1981 4887
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2945 3165 1184
Size: 1761 220 4887
Fragments arranged by size:
4887 1761 220
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2519 2551 2519
Size: 32 6836
AlwNI CAG_nnn'CTG
Cuts at: 3063 3139 4611 6183 3063
Size: 76 1472 1572 3748
Fragments arranged by size:
3748 1572 1472 76
ApaLI G'TGCA_C
Cuts at: 2720 4925 6081 2720
Size: 2205 1156 3507
Fragments arranged by size:
3507 2205 1156
ApoI r'AATT_y
Cuts at: 1493 4177 4403 4555 6664 1493
Size: 2684 226 152 2109 1697
Fragments arranged by size:
2684 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5422 160
Size: 872 4390 1606
Fragments arranged by size:
4390 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6868
AvaI C'yCGr_G
Cuts at: 1870 1943 3500 3764 4193 1870
Size: 73 1557 264 429 4545
Fragments arranged by size:
4545 1557 429 264 73
BamHI G'GATC_C
Cuts at: 4198 4565 4198
Size: 367 6501
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2181 2741 2984 3190 4189
Size: 325 217 627 394 560 243 206 999
Cuts at: 4189 5644 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 999 627 560 394 325 243
217 206
BanII G_rGCy'C
Cuts at: 1391 3984 4077 4187 1391
Size: 2593 93 110 4072
Fragments arranged by size:
4072 2593 110 93
BbeI G_GCGC'C
Cuts at: 2185 2185
Size: 6868
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2990 3843 928
Size: 971 1091 853 3953
Fragments arranged by size:
3953 1091 971 853
BbvCI CC'TCA_GC
Cuts at: 2171 2666 2974 2171
Size: 495 308 6065
Fragments arranged by size:
6065 495 308
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5059 5093 1903
Size: 34 3122 34 3678
Fragments arranged by size:
3678 3122 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3414 4760 5970 3414
Size: 1346 1210 4312
Fragments arranged by size:
4312 1346 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6868
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3054 5479 136
Size: 107 122 71 631 605 1382 2425 1525
Fragments arranged by size:
2425 1525 1382 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3570 6863 3570
Size: 3293 3575
BlpI GC'TnA_GC
Cuts at: 2553 2681 4285 2553
Size: 128 1604 5136
Fragments arranged by size:
5136 1604 128
Bme1580I G_kGCm'C
Cuts at: 1790 2724 2844 2987 4929 6085 1790
Size: 934 120 143 1942 1156 2573
Fragments arranged by size:
2573 1942 1156 934 143 120
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6868
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2386 3489 4599
Size: 416 53 83 399 24 969 1103 1110
Cuts at: 4599 5557 442
Size: 958 1753
Fragments arranged by size:
1753 1110 1103 969 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6868
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2220 2310 3326 3458 3694 3908 4061 5528
Size: 981 90 1016 132 236 214 153 1467
Cuts at: 5528 1239
Size: 2579
Fragments arranged by size:
2579 1467 1016 981 236 214 153 132
90
Bpu10I CC'TnA_GC
Cuts at: 2171 2666 2974 3818 3831 2171
Size: 495 308 844 13 5208
Fragments arranged by size:
5208 844 495 308 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2744 4993 5858 6156 2744
Size: 2249 865 298 3456
Fragments arranged by size:
3456 2249 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2467 2591 3420 5531 882
Size: 617 968 124 829 2111 2219
Fragments arranged by size:
2219 2111 968 829 617 124
BsaAI yAC'GTr
Cuts at: 493 1204 2725 4659 493
Size: 711 1521 1934 2702
Fragments arranged by size:
2702 1934 1521 711
BsaBI GATnn'nnATC
Cuts at: 3965 6862 3965
Size: 2897 3971
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2182 2995 4675 5057
Size: 53 83 186 936 649 813 1680 382
Cuts at: 5057 5692 275
Size: 635 1451
Fragments arranged by size:
1680 1451 936 813 649 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2987 5301 5699 5973 6120 6531 2987
Size: 2314 398 274 147 411 3324
Fragments arranged by size:
3324 2314 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2555 2585 3153 3183 1178
Size: 30 165 30 1152 30 568 30 4863
Fragments arranged by size:
4863 1152 568 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2165 2209 2393 2471 3038 4012 1186
Size: 979 44 184 78 567 974 4042
Fragments arranged by size:
4042 979 974 567 184 78 44
BseYI C'CCAG_C
Cuts at: 3220 3232 3539 6071 6567 3220
Size: 12 307 2532 496 3521
Fragments arranged by size:
3521 2532 496 307 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2265 2388 3125 3635 3671 1163
Size: 573 529 123 737 510 36 4360
Fragments arranged by size:
4360 737 573 529 510 123 36
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2869 4318 5079 6107 1540
Size: 275 186 868 1449 761 1028 2301
Fragments arranged by size:
2301 1449 1028 868 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2157 2724 3154 4929 5014 6085 2157
Size: 567 430 1775 85 1071 2940
Fragments arranged by size:
2940 1775 1071 567 430 85
BsmI GAATG_Cn'
Cuts at: 4379 4472 4379
Size: 93 6775
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 2724 2844 2987 3154 3984 4077
Size: 399 367 567 120 143 167 830 93
Cuts at: 4077 4187 4929 5014 6085 1391
Size: 110 742 85 1071 2174
Fragments arranged by size:
2174 1071 830 742 567 399 367 167
143 120 110 93 85
BspEI T'CCGG_A
Cuts at: 6531 6531
Size: 6868
BspHI T'CATG_A
Cuts at: 4757 6487 4757
Size: 1730 5138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2428 2449 2686 3531 4223 844
Size: 1584 21 237 845 692 3489
Fragments arranged by size:
3489 1584 845 692 237 21
BsrBI CCG'CTC
Cuts at: 1394 1823 4755 1394
Size: 429 2932 3507
Fragments arranged by size:
3507 2932 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3840 5363 5537 65
Size: 1183 2592 1523 174 1396
Fragments arranged by size:
2592 1523 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2716 2864 2987 3193 5512 5699 1700
Size: 110 906 148 123 206 2319 187 2869
Fragments arranged by size:
2869 2319 906 206 187 148 123 110
BsrGI T'GTAC_A
Cuts at: 96 3640 96
Size: 3544 3324
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6868
BssSI C'ACGA_G
Cuts at: 4928 5940 4928
Size: 1012 5856
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3139 794
Size: 2345 4523
BstBI TT'CG_AA
Cuts at: 5672 5672
Size: 6868
BstEII G'GTnAC_C
Cuts at: 1184 2959 1184
Size: 1775 5093
BstXI CCAn_nnnn'nTGG
Cuts at: 3361 3361
Size: 6868
BstYI r'GATC_y
Cuts at: 1972 3066 3570 4198 4565 4950 4967 6419 6505
Size: 1094 504 628 367 385 17 1452 86
Cuts at: 6505 6517 6863 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1094 628 504 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3792 3945 4143 513
Size: 552 939 1788 153 198 3238
Fragments arranged by size:
3238 1788 939 552 198 153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2947 487
Size: 683 56 1721 4408
Fragments arranged by size:
4408 1721 683 56
BtsI GCAGTG_nn'
Cuts at: 2277 2376 2498 2861 3243 3373 3618 4384 5178
Size: 99 122 363 382 130 245 766 794
Cuts at: 5178 5198 2277
Size: 20 3947
Fragments arranged by size:
3947 794 766 382 363 245 130 122
99 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6868
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4050 4085 529
Size: 35 3486 35 3312
Fragments arranged by size:
3486 3312 35 35
DraI TTT'AAA
Cuts at: 4172 4172
Size: 6868
DraIII CAC_nnn'GTG
Cuts at: 3087 4038 3087
Size: 951 5917
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5875 809
Size: 507 4559 1802
Fragments arranged by size:
4559 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2437 2638 2866
Size: 54 1425 50 275 18 607 201 228
Cuts at: 2866 2890 4315 5204 6833 8
Size: 24 1425 889 1629 43
Fragments arranged by size:
1629 1425 1425 889 607 275 228 201
54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2866 4315 1537
Size: 275 1054 1449 4090
Fragments arranged by size:
4090 1449 1054 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3423 4798 1353
Size: 2070 1375 3423
Fragments arranged by size:
3423 2070 1375
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5455 5829 5975 387
Size: 1064 138 3866 374 146 1280
Fragments arranged by size:
3866 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2220 2282 2310 2561 3017 3326 3404
Size: 393 588 62 28 251 456 309 78
Cuts at: 3404 3429 3458 3694 3908 4061 4931 5528 6315
Size: 25 29 236 214 153 870 597 787
Cuts at: 6315 6583 1239
Size: 268 1524
Fragments arranged by size:
1524 870 787 597 588 456 393 309
268 251 236 214 153 78 62 29
28 25
EcoNI CCTnn'n_nnAGG
Cuts at: 2264 3159 2264
Size: 895 5973
EcoO109I rG'GnC_Cy
Cuts at: 2167 2316 2773 2811 3177 2167
Size: 149 457 38 366 5858
Fragments arranged by size:
5858 457 366 149 38
EcoRI G'AATT_C
Cuts at: 4177 4555 4177
Size: 378 6490
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3434 3434
Size: 6868
FspI TGC'GCA
Cuts at: 5374 5374
Size: 6868
HaeII r_GCGC'y
Cuts at: 1678 2032 2185 2830 6015 1678
Size: 354 153 645 3185 2531
Fragments arranged by size:
3185 2531 645 354 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2378 2410 3045 3077 3153
Size: 32 135 32 806 32 635 32 76
Cuts at: 3153 3185 3496 3528 5509 5541 5583 5615 1373
Size: 32 311 32 1981 32 42 32 2626
Fragments arranged by size:
2626 1981 806 635 311 135 76 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3312 4212 4458 669
Size: 988 281 1374 900 246 3079
Fragments arranged by size:
3079 1374 988 900 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6868
HpaI GTT'AAC
Cuts at: 4458 4458
Size: 6868
KasI G'GCGC_C
Cuts at: 2181 2181
Size: 6868
KpnI G_GTAC'C
Cuts at: 4193 4193
Size: 6868
MfeI C'AATT_G
Cuts at: 3624 4467 3624
Size: 843 6025
MluI A'CGCG_T
Cuts at: 6744 6744
Size: 6868
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3345 3837 5982 6166 1305
Size: 2040 492 2145 184 2007
Fragments arranged by size:
2145 2040 2007 492 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2439 2640 2892 10
Size: 54 1425 343 607 201 252 3986
Fragments arranged by size:
3986 1425 607 343 252 201 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2408 3147 3791 3872 4826 5185 5344
Size: 648 1242 739 644 81 954 359 159
Cuts at: 5344 518
Size: 2042
Fragments arranged by size:
2042 1242 954 739 648 644 359 159
81
MspA1I CmG'CkG
Cuts at: 1606 2606 3375 3619 4166 4284 4961 6109 6354
Size: 1000 769 244 547 118 677 1148 245
Cuts at: 6354 1606
Size: 2120
Fragments arranged by size:
2120 1148 1000 769 677 547 245 244
118
NarI GG'CG_CC
Cuts at: 2182 2182
Size: 6868
NcoI C'CATG_G
Cuts at: 513 1065 2004 3792 3945 513
Size: 552 939 1788 153 3436
Fragments arranged by size:
3436 1788 939 552 153
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6868
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6868
NotI GC'GGCC_GC
Cuts at: 4315 4315
Size: 6868
NspI r_CATG'y
Cuts at: 1132 1229 4070 4111 4226 5771 1132
Size: 97 2841 41 115 1545 2229
Fragments arranged by size:
2841 2229 1545 115 97 41
PasI CC'CwG_GG
Cuts at: 1348 2107 3115 1348
Size: 759 1008 5101
Fragments arranged by size:
5101 1008 759
PciI A'CATG_T
Cuts at: 4066 4107 5767 4066
Size: 41 1660 5167
Fragments arranged by size:
5167 1660 41
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3672 1108
Size: 2564 4304
PfoI T'CCnGG_A
Cuts at: 2396 2396
Size: 6868
PmeI GTTT'AAAC
Cuts at: 4172 4172
Size: 6868
PmlI CAC'GTG
Cuts at: 2725 2725
Size: 6868
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4937 4969 6450 6482 4937
Size: 32 1481 32 5323
Fragments arranged by size:
5323 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2316 2773 3177 2316
Size: 457 404 6007
Fragments arranged by size:
6007 457 404
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6868
PsiI TTA'TAA
Cuts at: 4438 4438
Size: 6868
PstI C_TGCA'G
Cuts at: 830 2460 4220 830
Size: 1630 1760 3478
Fragments arranged by size:
3478 1760 1630
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6868
PvuII CAG'CTG
Cuts at: 2606 3619 4166 2606
Size: 1013 547 5308
Fragments arranged by size:
5308 1013 547
SalI G'TCGA_C
Cuts at: 1936 4210 1936
Size: 2274 4594
SbfI CC_TGCA'GG
Cuts at: 4220 4220
Size: 6868
ScaI AGT'ACT
Cuts at: 2567 5116 6828 2567
Size: 2549 1712 2607
Fragments arranged by size:
2607 2549 1712
SfcI C'TryA_G
Cuts at: 826 1045 2456 4003 4216 5351 6032 6223 826
Size: 219 1411 1547 213 1135 681 191 1471
Fragments arranged by size:
1547 1471 1411 1135 681 219 213 191
SfoI GGC'GCC
Cuts at: 2183 2183
Size: 6868
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6868
SgrAI Cr'CCGG_yG
Cuts at: 1700 2987 1700
Size: 1287 5581
SmaI CCC'GGG
Cuts at: 1872 4195 1872
Size: 2323 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2759 3500 4972 5873 6135 820
Size: 197 926 816 741 1472 901 262 1553
Fragments arranged by size:
1553 1472 926 901 816 741 262 197
SnaBI TAC'GTA
Cuts at: 493 4659 493
Size: 4166 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6868
SphI G_CATG'C
Cuts at: 1132 4226 1132
Size: 3094 3774
SspI AAT'ATT
Cuts at: 5 52 4792 5
Size: 47 4740 2081
Fragments arranged by size:
4740 2081 47
StuI AGG'CCT
Cuts at: 1517 2897 1517
Size: 1380 5488
StyI C'CwwG_G
Cuts at: 513 1065 2004 2163 3354 3792 3945 4307 513
Size: 552 939 159 1191 438 153 362 3074
Fragments arranged by size:
3074 1191 939 552 438 362 159 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2367 2729 2816 2890 3159 3553 4895
Size: 1255 787 362 87 74 269 394 1342
Cuts at: 4895 4912 5065 5250 5695 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1342 1255 787 445 394 362 269
185 153 87 74 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2223 2565 3640
Size: 274 80 33 51 1443 246 342 1075
Cuts at: 3640 5114 6826 96
Size: 1474 1712 138
Fragments arranged by size:
1712 1474 1443 1075 342 274 246 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2342 2374 3274 3306 3794 3826 6828 6860 2342
Size: 32 900 32 488 32 3002 32 2350
Fragments arranged by size:
3002 2350 900 488 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2429 1316
Size: 1113 5755
XbaI T'CTAG_A
Cuts at: 4204 4204
Size: 6868
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3122 3122
Size: 6868
XhoI C'TCGA_G
Cuts at: 1943 3500 1943
Size: 1557 5311
XmaI C'CCGG_G
Cuts at: 1870 4193 1870
Size: 2323 4545
XmnI GAAnn'nnTTC
Cuts at: 3773 4997 3773
Size: 1224 5644
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4676 276
Size: 53 83 186 936 3142 2468
Fragments arranged by size:
3142 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI