Restriction map for
pFN21AB5708
(Circular) (Six-base) MAPSORT of: pFN21AB5708.seq Check: 9405 from: 1 to: 6859
pFN21AB5708 6859 bp
With 182 enzymes: SgfI *
April 7, 2017 10:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3256 3256
Size: 6859
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4669 278
Size: 53 83 186 936 3133 2468
Fragments arranged by size:
3133 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4202 1937
Size: 2265 4594
Acc65I G'GTAC_C
Cuts at: 4180 4180
Size: 6859
AclI AA'CG_TT
Cuts at: 1563 4986 5359 1563
Size: 3423 373 3063
Fragments arranged by size:
3423 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2639 3109 3575 3762 3782 3888 4142 4922
Size: 1007 470 466 187 20 106 254 780
Cuts at: 4922 6306 6574 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1007 780 470 466 268 254
187 106 20
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6662
AflIII A'CryG_T
Cuts at: 1203 3339 5758 6735 1203
Size: 2136 2419 977 1327
Fragments arranged by size:
2419 2136 1327 977
AgeI A'CCGG_T
Cuts at: 5690 5690
Size: 6859
AhdI GACnn_n'nnGTC
Cuts at: 5588 5588
Size: 6859
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6859
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3123 3579 3869 1184
Size: 1939 456 290 4174
Fragments arranged by size:
4174 1939 456 290
AlwNI CAG_nnn'CTG
Cuts at: 3147 3470 3499 4602 6174 3147
Size: 323 29 1103 1572 3832
Fragments arranged by size:
3832 1572 1103 323 29
ApaI G_GGCC'C
Cuts at: 2174 2508 2174
Size: 334 6525
ApaLI G'TGCA_C
Cuts at: 4916 6072 4916
Size: 1156 5703
ApoI r'AATT_y
Cuts at: 1493 4168 4394 4546 6655 1493
Size: 2675 226 152 2109 1697
Fragments arranged by size:
2675 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5413 160
Size: 872 4381 1606
Fragments arranged by size:
4381 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6859
AvaI C'yCGr_G
Cuts at: 1870 1943 2137 2591 4184 1870
Size: 73 194 454 1593 4545
Fragments arranged by size:
4545 1593 454 194 73
BamHI G'GATC_C
Cuts at: 4189 4556 4189
Size: 367 6492
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2141 2179 2243 2270 4180
Size: 325 217 627 354 38 64 27 1910
Cuts at: 4180 5635 618
Size: 1455 1842
Fragments arranged by size:
1910 1842 1455 627 354 325 217 64
38 27
BanII G_rGCy'C
Cuts at: 1391 2174 2301 2508 2750 2834 4150 4178 1391
Size: 783 127 207 242 84 1316 28 4072
Fragments arranged by size:
4072 1316 783 242 207 127 84 28
BbeI G_GCGC'C
Cuts at: 2145 2183 2247 2274 2145
Size: 38 64 27 6730
Fragments arranged by size:
6730 64 38 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5888
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5050 5084 1903
Size: 34 3113 34 3678
Fragments arranged by size:
3678 3113 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4127 4751 5961 4127
Size: 624 1210 5025
Fragments arranged by size:
5025 1210 624
BclI T'GATC_A
Cuts at: 1548 3040 1548
Size: 1492 5367
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3152 5470 136
Size: 107 122 71 631 605 1480 2318 1525
Fragments arranged by size:
2318 1525 1480 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6854 6854
Size: 6859
BlpI GC'TnA_GC
Cuts at: 4276 4276
Size: 6859
Bme1580I G_kGCm'C
Cuts at: 1790 2174 2508 3168 3379 3517 4920 6076 1790
Size: 384 334 660 211 138 1403 1156 2573
Fragments arranged by size:
2573 1403 1156 660 384 334 211 138
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6859
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2950 3906 4590
Size: 416 53 83 399 24 1533 956 684
Cuts at: 4590 5548 442
Size: 958 1753
Fragments arranged by size:
1753 1533 958 956 684 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6859
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3193 3193
Size: 6859
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3070 3698 5519 1239
Size: 1831 628 1821 2579
Fragments arranged by size:
2579 1831 1821 628
Bpu10I CC'TnA_GC
Cuts at: 2099 2099
Size: 6859
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3659 4984 5849 6147 3659
Size: 1325 865 298 4371
Fragments arranged by size:
4371 1325 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5522 882
Size: 617 4023 2219
Fragments arranged by size:
4023 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4650 493
Size: 711 3446 2702
Fragments arranged by size:
3446 2702 711
BsaBI GATnn'nnATC
Cuts at: 2613 6853 2613
Size: 4240 2619
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2142 2151 2180 2244
Size: 53 83 186 936 609 9 29 64
Cuts at: 2244 2271 4666 5048 5683 275
Size: 27 2395 382 635 1451
Fragments arranged by size:
2395 1451 936 635 609 382 186 83
64 53 29 27 9
BsaWI w'CCGG_w
Cuts at: 5292 5690 5964 6111 6522 5292
Size: 398 274 147 411 5629
Fragments arranged by size:
5629 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2243 2273 2772 2802 3801
Size: 30 165 30 840 30 499 30 999
Cuts at: 3801 3831 1178
Size: 30 4206
Fragments arranged by size:
4206 999 840 499 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2194 2206 1186
Size: 1008 12 5839
Fragments arranged by size:
5839 1008 12
BseYI C'CCAG_C
Cuts at: 3137 3537 6062 6558 3137
Size: 400 2525 496 3438
Fragments arranged by size:
3438 2525 496 400
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3512 3699 1163
Size: 573 1776 187 4323
Fragments arranged by size:
4323 1776 573 187
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2261 4309 5070 6098 1540
Size: 275 186 260 2048 761 1028 2301
Fragments arranged by size:
2301 2048 1028 761 275 260 186
BsiHKAI G_wGCw'C
Cuts at: 2750 3438 4150 4920 5005 6076 2750
Size: 688 712 770 85 1071 3533
Fragments arranged by size:
3533 1071 770 712 688 85
BsmI GAATG_Cn'
Cuts at: 2554 2687 3412 3937 4370 4463 2554
Size: 133 725 525 433 93 4950
Fragments arranged by size:
4950 725 525 433 133 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2174 2301 2508 2750 2834 3168 3379
Size: 399 384 127 207 242 84 334 211
Cuts at: 3379 3438 3517 4150 4178 4920 5005 6076 1391
Size: 59 79 633 28 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 633 399 384 334 242
211 207 127 85 84 79 59 28
BspEI T'CCGG_A
Cuts at: 6522 6522
Size: 6859
BspHI T'CATG_A
Cuts at: 2079 4748 6478 2079
Size: 2669 1730 2460
Fragments arranged by size:
2669 2460 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2536 2776 3256 4214 844
Size: 1692 240 480 958 3489
Fragments arranged by size:
3489 1692 958 480 240
BsrBI CCG'CTC
Cuts at: 1394 1823 2092 4746 1394
Size: 429 269 2654 3507
Fragments arranged by size:
3507 2654 429 269
BsrDI GCAATG_nn'
Cuts at: 65 1248 5354 5528 65
Size: 1183 4106 174 1396
Fragments arranged by size:
4106 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5503 5690 1700
Size: 110 3693 187 2869
Fragments arranged by size:
3693 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6859
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6859
BssSI C'ACGA_G
Cuts at: 3167 4919 5931 3167
Size: 1752 1012 4095
Fragments arranged by size:
4095 1752 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3470 794
Size: 2676 4183
BstBI TT'CG_AA
Cuts at: 5663 5663
Size: 6859
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6859
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6859
BstYI r'GATC_y
Cuts at: 1972 3496 3634 4107 4189 4556 4941 4958 6410
Size: 1524 138 473 82 367 385 17 1452
Cuts at: 6410 6496 6508 6854 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1524 1452 473 385 367 346 138
86 82 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2035 2247 2730 2776 3156 513
Size: 552 970 212 483 46 380 4216
Fragments arranged by size:
4216 970 552 483 380 212 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6120
Fragments arranged by size:
6120 683 56
BtsI GCAGTG_nn'
Cuts at: 2647 2768 3197 3471 3618 3954 4375 5169 5189
Size: 121 429 274 147 336 421 794 20
Cuts at: 5189 2647
Size: 4317
Fragments arranged by size:
4317 794 429 421 336 274 147 121
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6859
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6824
DraI TTT'AAA
Cuts at: 4163 4163
Size: 6859
DraIII CAC_nnn'GTG
Cuts at: 2441 2441
Size: 6859
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5866 809
Size: 507 4550 1802
Fragments arranged by size:
4550 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2410 3841 4306
Size: 54 1425 50 275 18 580 1431 465
Cuts at: 4306 5195 6824 8
Size: 889 1629 43
Fragments arranged by size:
1629 1431 1425 889 580 465 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4306 1537
Size: 275 2494 4090
Fragments arranged by size:
4090 2494 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3190 4789 1353
Size: 1837 1599 3423
Fragments arranged by size:
3423 1837 1599
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2055 4001 5446 5820 5966 387
Size: 1064 138 466 1946 1445 374 146 1280
Fragments arranged by size:
1946 1445 1280 1064 466 374 146 138
EcoICRI GAG'CTC
Cuts at: 2748 4148 2748
Size: 1400 5459
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2639 3070 3109 3575 3698 3762 3782
Size: 393 1007 431 39 466 123 64 20
Cuts at: 3782 3888 4142 4922 5519 6306 6574 1239
Size: 106 254 780 597 787 268 1524
Fragments arranged by size:
1524 1007 787 780 597 466 431 393
268 254 123 106 64 39 20
EcoO109I rG'GnC_Cy
Cuts at: 2171 2330 2504 2171
Size: 159 174 6526
Fragments arranged by size:
6526 174 159
EcoRI G'AATT_C
Cuts at: 4168 4546 4168
Size: 378 6481
FspI TGC'GCA
Cuts at: 5365 5365
Size: 6859
HaeII r_GCGC'y
Cuts at: 1678 2145 2183 2247 2274 2287 3147 6006 1678
Size: 467 38 64 27 13 860 2859 2531
Fragments arranged by size:
2859 2531 860 467 64 38 27 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2201 2233 2243 2275 2501
Size: 32 135 32 629 32 10 32 226
Cuts at: 2501 2533 2560 2592 2772 2804 4092 4124 5500
Size: 32 27 32 180 32 1288 32 1376
Cuts at: 5500 5532 5574 5606 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1376 1288 629 226 180 135 42
32 32 32 32 32 32 32 32
32 32 27 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3993 4203 4449 669
Size: 988 281 2055 210 246 3079
Fragments arranged by size:
3079 2055 988 281 246 210
HindIII A'AGCT_T
Cuts at: 748 3019 748
Size: 2271 4588
HpaI GTT'AAC
Cuts at: 4449 4449
Size: 6859
KasI G'GCGC_C
Cuts at: 2141 2179 2243 2270 2141
Size: 38 64 27 6730
Fragments arranged by size:
6730 64 38 27
KpnI G_GTAC'C
Cuts at: 4184 4184
Size: 6859
MfeI C'AATT_G
Cuts at: 4458 4458
Size: 6859
MluI A'CGCG_T
Cuts at: 6735 6735
Size: 6859
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2223 2232 4135 5973 6157 1305
Size: 918 9 1903 1838 184 2007
Fragments arranged by size:
2007 1903 1838 918 184 9
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2412 10
Size: 54 1425 343 580 4457
Fragments arranged by size:
4457 1425 580 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2619 3561 4817 5176 5335 518
Size: 648 843 610 942 1256 359 159 2042
Fragments arranged by size:
2042 1256 942 843 648 610 359 159
MspA1I CmG'CkG
Cuts at: 1606 2037 2157 3815 3866 4275 4952 6100 6345
Size: 431 120 1658 51 409 677 1148 245
Cuts at: 6345 1606
Size: 2120
Fragments arranged by size:
2120 1658 1148 677 431 409 245 120
51
NarI GG'CG_CC
Cuts at: 2142 2180 2244 2271 2142
Size: 38 64 27 6730
Fragments arranged by size:
6730 64 38 27
NcoI C'CATG_G
Cuts at: 513 1065 2776 3156 513
Size: 552 1711 380 4216
Fragments arranged by size:
4216 1711 552 380
NdeI CA'TA_TG
Cuts at: 387 2304 387
Size: 1917 4942
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6859
NotI GC'GGCC_GC
Cuts at: 4306 4306
Size: 6859
NspI r_CATG'y
Cuts at: 1132 1229 2891 3421 3560 4217 5762 1132
Size: 97 1662 530 139 657 1545 2229
Fragments arranged by size:
2229 1662 1545 657 530 139 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6859
PciI A'CATG_T
Cuts at: 5758 5758
Size: 6859
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2419 1108
Size: 1311 5548
PmeI GTTT'AAAC
Cuts at: 4163 4163
Size: 6859
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2964 2996 4928 4960 6441 6473 2964
Size: 32 1932 32 1481 32 3350
Fragments arranged by size:
3350 1932 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6859
PsiI TTA'TAA
Cuts at: 4429 4429
Size: 6859
PspOMI G'GGCC_C
Cuts at: 2170 2504 2170
Size: 334 6525
PstI C_TGCA'G
Cuts at: 830 4211 830
Size: 3381 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6859
PvuII CAG'CTG
Cuts at: 2157 3866 2157
Size: 1709 5150
SacI G_AGCT'C
Cuts at: 2750 4150 2750
Size: 1400 5459
SacII CC_GC'GG
Cuts at: 2038 2038
Size: 6859
SalI G'TCGA_C
Cuts at: 1936 4201 1936
Size: 2265 4594
SbfI CC_TGCA'GG
Cuts at: 4211 4211
Size: 6859
ScaI AGT'ACT
Cuts at: 4045 5107 6819 4045
Size: 1062 1712 4085
Fragments arranged by size:
4085 1712 1062
SfcI C'TryA_G
Cuts at: 826 1045 4207 5342 6023 6214 826
Size: 219 3162 1135 681 191 1471
Fragments arranged by size:
3162 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2143 2181 2245 2272 2143
Size: 38 64 27 6730
Fragments arranged by size:
6730 64 38 27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6859
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6859
SmaI CCC'GGG
Cuts at: 1872 2139 2593 4186 1872
Size: 267 454 1593 4545
Fragments arranged by size:
4545 1593 454 267
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3638 4963 5864 6126 820
Size: 197 926 1695 1325 901 262 1553
Fragments arranged by size:
1695 1553 1325 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4650 493
Size: 4157 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6859
SphI G_CATG'C
Cuts at: 1132 4217 1132
Size: 3085 3774
SspI AAT'ATT
Cuts at: 5 52 3061 3298 4783 5
Size: 47 3009 237 1485 2081
Fragments arranged by size:
3009 2081 1485 237 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6859
StyI C'CwwG_G
Cuts at: 513 1065 2776 3156 3364 4298 513
Size: 552 1711 380 208 934 3074
Fragments arranged by size:
3074 1711 934 552 380 208
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2152 2927 4886 4903 5056 5241 5686
Size: 1255 572 775 1959 17 153 185 445
Cuts at: 5686 325
Size: 1498
Fragments arranged by size:
1959 1498 1255 775 572 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4043 5105 6817
Size: 274 80 33 51 1443 2066 1062 1712
Cuts at: 6817 96
Size: 138
Fragments arranged by size:
2066 1712 1443 1062 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3801 3833 6819 6851 3801
Size: 32 2986 32 3809
Fragments arranged by size:
3809 2986 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6859
XbaI T'CTAG_A
Cuts at: 4195 4195
Size: 6859
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2309 2309
Size: 6859
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6859
XmaI C'CCGG_G
Cuts at: 1870 2137 2591 4184 1870
Size: 267 454 1593 4545
Fragments arranged by size:
4545 1593 454 267
XmnI GAAnn'nnTTC
Cuts at: 2610 3888 4988 2610
Size: 1278 1100 4481
Fragments arranged by size:
4481 1278 1100
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4667 276
Size: 53 83 186 936 3133 2468
Fragments arranged by size:
3133 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI
BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI
SwaI