Restriction map for
pFN21AB5702
(Circular) (Six-base) MAPSORT of: pFN21AB5702.seq Check: 5841 from: 1 to: 7423
pFN21AB5702 7423 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5233 278
Size: 53 83 186 936 3697 2468
Fragments arranged by size:
3697 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2445 3142 3705 4766 1937
Size: 508 697 563 1061 4594
Fragments arranged by size:
4594 1061 697 563 508
Acc65I G'GTAC_C
Cuts at: 4744 4744
Size: 7423
AclI AA'CG_TT
Cuts at: 1563 2655 5550 5923 1563
Size: 1092 2895 373 3063
Fragments arranged by size:
3063 2895 1092 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2571 2875 4125 5486 6870 7138 1632
Size: 939 304 1250 1361 1384 268 1917
Fragments arranged by size:
1917 1384 1361 1250 939 304 268
AfeI AGC'GCT
Cuts at: 2903 2903
Size: 7423
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7226
AflIII A'CryG_T
Cuts at: 1203 2978 6322 7299 1203
Size: 1775 3344 977 1327
Fragments arranged by size:
3344 1775 1327 977
AgeI A'CCGG_T
Cuts at: 6254 6254
Size: 7423
AhdI GACnn_n'nnGTC
Cuts at: 6152 6152
Size: 7423
AleI CACnn'nnGTG
Cuts at: 1166 2817 1166
Size: 1651 5772
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4318 1184
Size: 3134 4289
AlwNI CAG_nnn'CTG
Cuts at: 3034 3043 3506 4330 5166 6738 3034
Size: 9 463 824 836 1572 3719
Fragments arranged by size:
3719 1572 836 824 463 9
ApaI G_GGCC'C
Cuts at: 2145 2145
Size: 7423
ApaLI G'TGCA_C
Cuts at: 5480 6636 5480
Size: 1156 6267
ApoI r'AATT_y
Cuts at: 1493 2215 4732 4958 5110 7219 1493
Size: 722 2517 226 152 2109 1697
Fragments arranged by size:
2517 2109 1697 722 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3399 5977 160
Size: 872 2367 2578 1606
Fragments arranged by size:
2578 2367 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7423
AvaI C'yCGr_G
Cuts at: 1870 1943 2388 3654 4011 4194 4748 1870
Size: 73 445 1266 357 183 554 4545
Fragments arranged by size:
4545 1266 554 445 357 183 73
BamHI G'GATC_C
Cuts at: 2044 4262 4753 5120 2044
Size: 2218 491 367 4347
Fragments arranged by size:
4347 2218 491 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4591 4744 6199 618
Size: 325 217 627 2804 153 1455 1842
Fragments arranged by size:
2804 1842 1455 627 325 217 153
BanII G_rGCy'C
Cuts at: 1391 2145 4742 1391
Size: 754 2597 4072
Fragments arranged by size:
4072 2597 754
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3131 3987 928
Size: 971 1232 856 4364
Fragments arranged by size:
4364 1232 971 856
BbvCI CC'TCA_GC
Cuts at: 3797 4254 4694 3797
Size: 457 440 6526
Fragments arranged by size:
6526 457 440
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5614 5648 1903
Size: 34 3677 34 3678
Fragments arranged by size:
3678 3677 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5315 6525 5315
Size: 1210 6213
BclI T'GATC_A
Cuts at: 1548 2617 1548
Size: 1069 6354
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2717 2960 3311
Size: 107 122 71 631 605 1045 243 351
Cuts at: 3311 6034 136
Size: 2723 1525
Fragments arranged by size:
2723 1525 1045 631 605 351 243 122
107 71
BglII A'GATC_T
Cuts at: 7418 7418
Size: 7423
BlpI GC'TnA_GC
Cuts at: 4026 4840 4026
Size: 814 6609
Bme1580I G_kGCm'C
Cuts at: 1790 2145 4012 5484 6640 1790
Size: 355 1867 1472 1156 2573
Fragments arranged by size:
2573 1867 1472 1156 355
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7423
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5154 6112 442
Size: 416 53 83 399 24 3737 958 1753
Fragments arranged by size:
3737 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7423
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2414 2414
Size: 7423
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2939 6083 1239
Size: 1700 3144 2579
Fragments arranged by size:
3144 2579 1700
Bpu10I CC'TnA_GC
Cuts at: 3797 4254 4694 3797
Size: 457 440 6526
Fragments arranged by size:
6526 457 440
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2246 2905 5548 6413 6711 2246
Size: 659 2643 865 298 2958
Fragments arranged by size:
2958 2643 865 659 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6086 882
Size: 617 4587 2219
Fragments arranged by size:
4587 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3667 4675 5214 493
Size: 711 2463 1008 539 2702
Fragments arranged by size:
2702 2463 1008 711 539
BsaBI GATnn'nnATC
Cuts at: 7417 7417
Size: 7423
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5230 5612 6247 275
Size: 53 83 186 936 3697 382 635 1451
Fragments arranged by size:
3697 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5856 6254 6528 6675 7086 5856
Size: 398 274 147 411 6193
Fragments arranged by size:
6193 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2240 2270 1178
Size: 30 165 30 837 30 6331
Fragments arranged by size:
6331 837 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2282 2838 2841 2844 2912 2936 3926 1186
Size: 1096 556 3 3 68 24 990 4683
Fragments arranged by size:
4683 1096 990 556 68 24 3 3
BseYI C'CCAG_C
Cuts at: 3813 6626 7122 3813
Size: 2813 496 4114
Fragments arranged by size:
4114 2813 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2959 3390 4424 4514 4706 1163
Size: 573 1223 431 1034 90 192 3880
Fragments arranged by size:
3880 1223 1034 573 431 192 90
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4873 5634 6662 1540
Size: 275 186 2872 761 1028 2301
Fragments arranged by size:
2872 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5484 5569 6640 5484
Size: 85 1071 6267
Fragments arranged by size:
6267 1071 85
BsmI GAATG_Cn'
Cuts at: 2506 4489 4934 5027 2506
Size: 1983 445 93 4902
Fragments arranged by size:
4902 1983 445 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 4012 4742 5484 5569 6640 1391
Size: 399 355 1867 730 742 85 1071 2174
Fragments arranged by size:
2174 1867 1071 742 730 399 355 85
BspEI T'CCGG_A
Cuts at: 7086 7086
Size: 7423
BspHI T'CATG_A
Cuts at: 5312 7042 5312
Size: 1730 5693
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3051 4338 4350 4778 844
Size: 2207 1287 12 428 3489
Fragments arranged by size:
3489 2207 1287 428 12
BsrBI CCG'CTC
Cuts at: 1394 1823 5310 1394
Size: 429 3487 3507
Fragments arranged by size:
3507 3487 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2515 3070 3585 4138 4290 4300 5918
Size: 1183 1267 555 515 553 152 10 1618
Cuts at: 5918 6092 65
Size: 174 1396
Fragments arranged by size:
1618 1396 1267 1183 555 553 515 174
152 10
BsrFI r'CCGG_y
Cuts at: 1700 1810 6067 6254 1700
Size: 110 4257 187 2869
Fragments arranged by size:
4257 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7423
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7423
BssSI C'ACGA_G
Cuts at: 5483 6495 5483
Size: 1012 6411
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3043 4330 794
Size: 2249 1287 3887
Fragments arranged by size:
3887 2249 1287
BstBI TT'CG_AA
Cuts at: 2296 3355 6227 2296
Size: 1059 2872 3492
Fragments arranged by size:
3492 2872 1059
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7423
BstYI r'GATC_y
Cuts at: 1972 2044 3457 4262 4753 5120 5505 5522 6974
Size: 72 1413 805 491 367 385 17 1452
Cuts at: 6974 7060 7072 7418 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1413 805 491 385 367 346
86 72 17 12
Bsu36I CC'TnA_GG
Cuts at: 3390 3909 3390
Size: 519 6904
BtgI C'CryG_G
Cuts at: 513 1065 3786 3819 513
Size: 552 2721 33 4117
Fragments arranged by size:
4117 2721 552 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4336 487
Size: 683 56 3110 3574
Fragments arranged by size:
3574 3110 683 56
BtsI GCAGTG_nn'
Cuts at: 2787 3894 4939 5733 5753 2787
Size: 1107 1045 794 20 4457
Fragments arranged by size:
4457 1107 1045 794 20
ClaI AT'CG_AT
Cuts at: 1554 3840 1554
Size: 2286 5137
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7388
DraI TTT'AAA
Cuts at: 3466 4727 3466
Size: 1261 6162
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6430 809
Size: 507 5114 1802
Fragments arranged by size:
5114 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2608 2961 4000
Size: 54 1425 50 275 18 778 353 1039
Cuts at: 4000 4068 4149 4282 4870 5759 7388 8
Size: 68 81 133 588 889 1629 43
Fragments arranged by size:
1629 1425 1039 889 778 588 353 275
133 81 68 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4870 1537
Size: 275 3058 4090
Fragments arranged by size:
4090 3058 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2150 2359 4610 5353 1353
Size: 797 209 2251 743 3423
Fragments arranged by size:
3423 2251 797 743 209
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3014 6010 6384 6530 387
Size: 1064 138 1425 2996 374 146 1280
Fragments arranged by size:
2996 1425 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2571 2875 2939 4125 5486 6083 6870
Size: 393 939 304 64 1186 1361 597 787
Cuts at: 6870 7138 1239
Size: 268 1524
Fragments arranged by size:
1524 1361 1186 939 787 597 393 304
268 64
EcoNI CCTnn'n_nnAGG
Cuts at: 4460 4460
Size: 7423
EcoO109I rG'GnC_Cy
Cuts at: 2141 2142 2728 3031 3040 3903 4327 4690 2141
Size: 1 586 303 9 863 424 363 4874
Fragments arranged by size:
4874 863 586 424 363 303 9 1
EcoRI G'AATT_C
Cuts at: 4732 5110 4732
Size: 378 7045
FspI TGC'GCA
Cuts at: 5929 5929
Size: 7423
HaeII r_GCGC'y
Cuts at: 1678 2905 4449 6570 1678
Size: 1227 1544 2121 2531
Fragments arranged by size:
2531 2121 1544 1227
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2053 2085 2336 2368 2789
Size: 32 135 32 481 32 251 32 421
Cuts at: 2789 2821 4009 4041 4247 4279 6064 6096 6138
Size: 32 1188 32 206 32 1785 32 42
Cuts at: 6138 6170 1373
Size: 32 2626
Fragments arranged by size:
2626 1785 1188 481 421 251 206 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4767 5013 669
Size: 988 281 2829 246 3079
Fragments arranged by size:
3079 2829 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3347 4277 748
Size: 2599 930 3894
Fragments arranged by size:
3894 2599 930
HpaI GTT'AAC
Cuts at: 5013 5013
Size: 7423
KpnI G_GTAC'C
Cuts at: 4748 4748
Size: 7423
MfeI C'AATT_G
Cuts at: 5022 5022
Size: 7423
MluI A'CGCG_T
Cuts at: 2978 7299 2978
Size: 4321 3102
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2070 3768 3897 6537 6721 1305
Size: 765 1698 129 2640 184 2007
Fragments arranged by size:
2640 2007 1698 765 184 129
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2610 4002 4070 4151 10
Size: 54 1425 343 778 1392 68 81 3282
Fragments arranged by size:
3282 1425 1392 778 343 81 68 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2700 2817 3752 4142 4169 5381 5740
Size: 648 1534 117 935 390 27 1212 359
Cuts at: 5740 5899 518
Size: 159 2042
Fragments arranged by size:
2042 1534 1212 935 648 390 359 159
117 27
MspA1I CmG'CkG
Cuts at: 1606 2464 2746 3629 3991 4372 4839 5516 6664
Size: 858 282 883 362 381 467 677 1148
Cuts at: 6664 6909 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 883 858 677 467 381 362
282 245
NcoI C'CATG_G
Cuts at: 513 1065 3819 513
Size: 552 2754 4117
Fragments arranged by size:
4117 2754 552
NdeI CA'TA_TG
Cuts at: 387 2842 387
Size: 2455 4968
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7423
NotI GC'GGCC_GC
Cuts at: 4870 4870
Size: 7423
NspI r_CATG'y
Cuts at: 1132 1229 2688 3284 4781 6326 1132
Size: 97 1459 596 1497 1545 2229
Fragments arranged by size:
2229 1545 1497 1459 596 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7423
PciI A'CATG_T
Cuts at: 6322 6322
Size: 7423
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2185 3820 1108
Size: 1077 1635 4711
Fragments arranged by size:
4711 1635 1077
PfoI T'CCnGG_A
Cuts at: 3186 3870 4077 4265 3186
Size: 684 207 188 6344
Fragments arranged by size:
6344 684 207 188
PmeI GTTT'AAAC
Cuts at: 4727 4727
Size: 7423
PmlI CAC'GTG
Cuts at: 4675 4675
Size: 7423
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2149 2181 5492 5524 7005 7037 2149
Size: 32 3311 32 1481 32 2535
Fragments arranged by size:
3311 2535 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2728 3040 3903 4327 2728
Size: 312 863 424 5824
Fragments arranged by size:
5824 863 424 312
PshAI GACnn'nnGTC
Cuts at: 1613 2012 3968 1613
Size: 399 1956 5068
Fragments arranged by size:
5068 1956 399
PsiI TTA'TAA
Cuts at: 4993 4993
Size: 7423
PspOMI G'GGCC_C
Cuts at: 2141 2141
Size: 7423
PspXI vC'TCGA_Gb
Cuts at: 2388 2388
Size: 7423
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2277 2309 3336 3368 2277
Size: 32 1027 32 6332
Fragments arranged by size:
6332 1027 32 32
PstI C_TGCA'G
Cuts at: 830 2329 2632 2649 2751 3039 4385 4775 830
Size: 1499 303 17 102 288 1346 390 3478
Fragments arranged by size:
3478 1499 1346 390 303 288 102 17
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7423
PvuII CAG'CTG
Cuts at: 2464 2746 3629 3991 4372 2464
Size: 282 883 362 381 5515
Fragments arranged by size:
5515 883 381 362 282
SalI G'TCGA_C
Cuts at: 1936 4765 1936
Size: 2829 4594
SapI GCTCTTCn'nnn_
Cuts at: 2359 2359
Size: 7423
SbfI CC_TGCA'GG
Cuts at: 3039 4775 3039
Size: 1736 5687
ScaI AGT'ACT
Cuts at: 5671 7383 5671
Size: 1712 5711
SfcI C'TryA_G
Cuts at: 826 1045 2283 2325 2628 2645 2690 2747 3035
Size: 219 1238 42 303 17 45 57 288
Cuts at: 3035 4381 4771 5906 6587 6778 826
Size: 1346 390 1135 681 191 1471
Fragments arranged by size:
1471 1346 1238 1135 681 390 303 288
219 191 57 45 42 17
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7423
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7423
SmaI CCC'GGG
Cuts at: 1872 3656 4013 4750 1872
Size: 1784 357 737 4545
Fragments arranged by size:
4545 1784 737 357
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2261 2388 2884 5527 6428 6690
Size: 197 926 318 127 496 2643 901 262
Cuts at: 6690 820
Size: 1553
Fragments arranged by size:
2643 1553 926 901 496 318 262 197
127
SnaBI TAC'GTA
Cuts at: 493 5214 493
Size: 4721 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7423
SphI G_CATG'C
Cuts at: 1132 2688 4781 1132
Size: 1556 2093 3774
Fragments arranged by size:
3774 2093 1556
SrfI GCCC'GGGC
Cuts at: 4013 4013
Size: 7423
SspI AAT'ATT
Cuts at: 5 52 5347 5
Size: 47 5295 2081
Fragments arranged by size:
5295 2081 47
StuI AGG'CCT
Cuts at: 1517 2911 1517
Size: 1394 6029
StyI C'CwwG_G
Cuts at: 513 1065 3819 4862 513
Size: 552 2754 1043 3074
Fragments arranged by size:
3074 2754 1043 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5450 5467 5620 5805 6250 325
Size: 1255 3870 17 153 185 445 1498
Fragments arranged by size:
3870 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3054 5669 7381
Size: 274 80 33 51 1443 1077 2615 1712
Cuts at: 7381 96
Size: 138
Fragments arranged by size:
2615 1712 1443 1077 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7383 7415 7383
Size: 32 7391
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7423
XbaI T'CTAG_A
Cuts at: 2056 4759 2056
Size: 2703 4720
XhoI C'TCGA_G
Cuts at: 1943 2388 1943
Size: 445 6978
XmaI C'CCGG_G
Cuts at: 1870 3654 4011 4748 1870
Size: 1784 357 737 4545
Fragments arranged by size:
4545 1784 737 357
XmnI GAAnn'nnTTC
Cuts at: 2292 5552 2292
Size: 3260 4163
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5231 276
Size: 53 83 186 936 3697 2468
Fragments arranged by size:
3697 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
RsrII SacI SacII SanDI SexAI SfiI SfoI SwaI
XcmI