Restriction map for
pFN21AB5697
(Circular) (Six-base) MAPSORT of: pFN21AB5697.seq Check: 8753 from: 1 to: 6685
pFN21AB5697 6685 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4495 278
Size: 53 83 186 936 2959 2468
Fragments arranged by size:
2959 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3573 4028 1937
Size: 1636 455 4594
Fragments arranged by size:
4594 1636 455
Acc65I G'GTAC_C
Cuts at: 3657 4006 3657
Size: 349 6336
AclI AA'CG_TT
Cuts at: 1563 4812 5185 1563
Size: 3249 373 3063
Fragments arranged by size:
3249 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2234 2883 3938 3959 4748 6132 6400 1632
Size: 602 649 1055 21 789 1384 268 1917
Fragments arranged by size:
1917 1384 1055 789 649 602 268 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6488
AflIII A'CryG_T
Cuts at: 1203 5584 6561 1203
Size: 4381 977 1327
Fragments arranged by size:
4381 1327 977
AgeI A'CCGG_T
Cuts at: 3474 5516 3474
Size: 2042 4643
AhdI GACnn_n'nnGTC
Cuts at: 5414 5414
Size: 6685
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6685
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6685
AlwNI CAG_nnn'CTG
Cuts at: 2600 4428 6000 2600
Size: 1828 1572 3285
Fragments arranged by size:
3285 1828 1572
ApaI G_GGCC'C
Cuts at: 3262 3392 3522 3262
Size: 130 130 6425
Fragments arranged by size:
6425 130 130
ApaLI G'TGCA_C
Cuts at: 4742 5898 4742
Size: 1156 5529
ApoI r'AATT_y
Cuts at: 1493 3764 3994 4220 4372 6481 1493
Size: 2271 230 226 152 2109 1697
Fragments arranged by size:
2271 2109 1697 230 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5239 160
Size: 872 4207 1606
Fragments arranged by size:
4207 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6685
AvaI C'yCGr_G
Cuts at: 1870 1943 2022 4010 1870
Size: 73 79 1988 4545
Fragments arranged by size:
4545 1988 79 73
BamHI G'GATC_C
Cuts at: 3799 4015 4382 3799
Size: 216 367 6102
Fragments arranged by size:
6102 367 216
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2957 3041 3268 3365 3657
Size: 325 217 627 1170 84 227 97 292
Cuts at: 3657 3905 4006 5461 618
Size: 248 101 1455 1842
Fragments arranged by size:
1842 1455 1170 627 325 292 248 227
217 101 97 84
BanII G_rGCy'C
Cuts at: 1391 3262 3336 3392 3522 3552 4004 1391
Size: 1871 74 56 130 30 452 4072
Fragments arranged by size:
4072 1871 452 130 74 56 30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3046 3746 928
Size: 971 1147 700 3867
Fragments arranged by size:
3867 1147 971 700
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4876 4910 1903
Size: 34 2939 34 3678
Fragments arranged by size:
3678 2939 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2540 4577 5787 2540
Size: 2037 1210 3438
Fragments arranged by size:
3438 2037 1210
BclI T'GATC_A
Cuts at: 1548 2514 1548
Size: 966 5719
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3266 3517
Size: 107 122 71 631 605 337 1257 251
Cuts at: 3517 5296 136
Size: 1779 1525
Fragments arranged by size:
1779 1525 1257 631 605 337 251 122
107 71
BglII A'GATC_T
Cuts at: 3405 3456 6680 3405
Size: 51 3224 3410
Fragments arranged by size:
3410 3224 51
BlpI GC'TnA_GC
Cuts at: 2075 4102 2075
Size: 2027 4658
Bme1580I G_kGCm'C
Cuts at: 1790 2903 3046 3262 3392 3522 4746 5902 1790
Size: 1113 143 216 130 130 1224 1156 2573
Fragments arranged by size:
2573 1224 1156 1113 216 143 130 130
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6685
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4416 5374 442
Size: 416 53 83 399 24 2999 958 1753
Fragments arranged by size:
2999 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6685
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2476 3257 3668 3745 3777 3790 3944 5345
Size: 1237 781 411 77 32 13 154 1401
Cuts at: 5345 1239
Size: 2579
Fragments arranged by size:
2579 1401 1237 781 411 154 77 32
13
Bpu10I CC'TnA_GC
Cuts at: 3546 3546
Size: 6685
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4810 5675 5973 4810
Size: 865 298 5522
Fragments arranged by size:
5522 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2282 2999 5348 882
Size: 617 783 717 2349 2219
Fragments arranged by size:
2349 2219 783 717 617
BsaAI yAC'GTr
Cuts at: 493 1204 3503 4476 493
Size: 711 2299 973 2702
Fragments arranged by size:
2702 2299 973 711
BsaBI GATnn'nnATC
Cuts at: 6679 6679
Size: 6685
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3497 4492 4874 5509
Size: 53 83 186 936 1964 995 382 635
Cuts at: 5509 275
Size: 1451
Fragments arranged by size:
1964 1451 995 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3474 5118 5516 5790 5937 6348 3474
Size: 1644 398 274 147 411 3811
Fragments arranged by size:
3811 1644 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3641 3671 1178
Size: 30 165 30 2238 30 4192
Fragments arranged by size:
4192 2238 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3605 1186
Size: 2419 4266
BseYI C'CCAG_C
Cuts at: 2529 3385 5888 6384 2529
Size: 856 2503 496 2830
Fragments arranged by size:
2830 2503 856 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2728 2902 3076 1163
Size: 573 992 174 174 4772
Fragments arranged by size:
4772 992 573 174 174
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2758 2977 3106 3157 4135 4896
Size: 275 186 757 219 129 51 978 761
Cuts at: 4896 5924 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 978 761 757 275 219 186
129 51
BsiHKAI G_wGCw'C
Cuts at: 2783 3243 3336 3552 4746 4831 5902 2783
Size: 460 93 216 1194 85 1071 3566
Fragments arranged by size:
3566 1194 1071 460 216 93 85
BsmI GAATG_Cn'
Cuts at: 3324 3841 4196 4289 3324
Size: 517 355 93 5720
Fragments arranged by size:
5720 517 355 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2783 2903 3046 3243 3262 3336 3392
Size: 399 993 120 143 197 19 74 56
Cuts at: 3392 3522 3552 4004 4746 4831 5902 1391
Size: 130 30 452 742 85 1071 2174
Fragments arranged by size:
2174 1071 993 742 452 399 197 143
130 120 85 74 56 30 19
BspEI T'CCGG_A
Cuts at: 6348 6348
Size: 6685
BspHI T'CATG_A
Cuts at: 2511 4574 6304 2511
Size: 2063 1730 2892
Fragments arranged by size:
2892 2063 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3879 4040 844
Size: 3035 161 3489
Fragments arranged by size:
3489 3035 161
BsrBI CCG'CTC
Cuts at: 1394 1823 4572 1394
Size: 429 2749 3507
Fragments arranged by size:
3507 2749 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5180 5354 65
Size: 1183 3932 174 1396
Fragments arranged by size:
3932 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2753 3101 3368 3474 5329 5516 1700
Size: 110 943 348 267 106 1855 187 2869
Fragments arranged by size:
2869 1855 943 348 267 187 110 106
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6685
BssHII G'CGCG_C
Cuts at: 1925 2028 1925
Size: 103 6582
BssSI C'ACGA_G
Cuts at: 2439 3200 3212 4745 5757 2439
Size: 761 12 1533 1012 3367
Fragments arranged by size:
3367 1533 1012 761 12
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6685
BstBI TT'CG_AA
Cuts at: 5489 5489
Size: 6685
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6685
BstXI CCAn_nnnn'nTGG
Cuts at: 3218 3235 3254 3646 3218
Size: 17 19 392 6257
Fragments arranged by size:
6257 392 19 17
BstYI r'GATC_y
Cuts at: 1972 3405 3444 3456 3627 3799 4015 4382 4767
Size: 1433 39 12 171 172 216 367 385
Cuts at: 4767 4784 6236 6322 6334 6680 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1433 385 367 346 216 172
171 86 39 17 12 12
BstZ17I GTA'TAC
Cuts at: 3574 3574
Size: 6685
Bsu36I CC'TnA_GG
Cuts at: 2045 2045
Size: 6685
BtgI C'CryG_G
Cuts at: 513 1065 2004 2847 3228 3448 3513 513
Size: 552 939 843 381 220 65 3685
Fragments arranged by size:
3685 939 843 552 381 220 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2136 2475 2556 2778 3421 3805
Size: 683 56 910 339 81 222 643 384
Cuts at: 3805 487
Size: 3367
Fragments arranged by size:
3367 910 683 643 384 339 222 81
56
BtsI GCAGTG_nn'
Cuts at: 2160 2471 3115 4201 4995 5015 2160
Size: 311 644 1086 794 20 3830
Fragments arranged by size:
3830 1086 794 644 311 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6685
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6650
DraI TTT'AAA
Cuts at: 3989 3989
Size: 6685
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3013 5692 809
Size: 507 1697 2679 1802
Fragments arranged by size:
2679 1802 1697 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2325 2524 2755
Size: 54 1425 50 275 18 495 199 231
Cuts at: 2755 3103 3220 3531 4132 5021 6650 8
Size: 348 117 311 601 889 1629 43
Fragments arranged by size:
1629 1425 889 601 495 348 311 275
231 199 117 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2755 3103 4132 1537
Size: 275 943 348 1029 4090
Fragments arranged by size:
4090 1029 943 348 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3935 4615 1353
Size: 2582 680 3423
Fragments arranged by size:
3423 2582 680
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3502 5272 5646 5792 387
Size: 1064 138 1913 1770 374 146 1280
Fragments arranged by size:
1913 1770 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3334 3550 3334
Size: 216 6469
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 2476 2883 3257 3668 3745 3777
Size: 393 602 242 407 374 411 77 32
Cuts at: 3777 3790 3938 3944 3959 4748 5345 6132 6400
Size: 13 148 6 15 789 597 787 268
Cuts at: 6400 1239
Size: 1524
Fragments arranged by size:
1524 789 787 602 597 411 407 393
374 268 242 148 77 32 15 13
6
EcoNI CCTnn'n_nnAGG
Cuts at: 3180 3180
Size: 6685
EcoO109I rG'GnC_Cy
Cuts at: 2039 3258 3259 3342 3389 3518 3519 3580 2039
Size: 1219 1 83 47 129 1 61 5144
Fragments arranged by size:
5144 1219 129 83 61 47 1 1
EcoRI G'AATT_C
Cuts at: 3994 4372 3994
Size: 378 6307
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2728 2836 3010 3184 2728
Size: 108 174 174 6229
Fragments arranged by size:
6229 174 174 108
FspI TGC'GCA
Cuts at: 5191 5191
Size: 6685
HaeII r_GCGC'y
Cuts at: 1678 2015 2054 3102 5832 1678
Size: 337 39 1048 2730 2531
Fragments arranged by size:
2730 2531 1048 337 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2251 2283 5326 5358 5400
Size: 32 135 32 679 32 3043 32 42
Cuts at: 5400 5432 1373
Size: 32 2626
Fragments arranged by size:
3043 2626 679 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4029 4275 669
Size: 988 281 2091 246 3079
Fragments arranged by size:
3079 2091 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6685
HpaI GTT'AAC
Cuts at: 4275 4275
Size: 6685
KpnI G_GTAC'C
Cuts at: 3661 4010 3661
Size: 349 6336
MfeI C'AATT_G
Cuts at: 4284 4284
Size: 6685
MluI A'CGCG_T
Cuts at: 6561 6561
Size: 6685
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3993 5799 5983 1305
Size: 2688 1806 184 2007
Fragments arranged by size:
2688 2007 1806 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2327 3222 3533 10
Size: 54 1425 343 495 895 311 3162
Fragments arranged by size:
3162 1425 895 495 343 311 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2541 2625 4643 5002 5161 518
Size: 648 1375 84 2018 359 159 2042
Fragments arranged by size:
2042 2018 1375 648 359 159 84
MspA1I CmG'CkG
Cuts at: 1606 2529 2742 2916 2949 4101 4778 5926 6171
Size: 923 213 174 33 1152 677 1148 245
Cuts at: 6171 1606
Size: 2120
Fragments arranged by size:
2120 1152 1148 923 677 245 213 174
33
NaeI GCC'GGC
Cuts at: 3103 3103
Size: 6685
NcoI C'CATG_G
Cuts at: 513 1065 2004 2847 3228 3513 513
Size: 552 939 843 381 285 3685
Fragments arranged by size:
3685 939 843 552 381 285
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6685
NgoMIV G'CCGG_C
Cuts at: 3101 3101
Size: 6685
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6685
NotI GC'GGCC_GC
Cuts at: 4132 4132
Size: 6685
NspI r_CATG'y
Cuts at: 1132 1229 4043 5588 1132
Size: 97 2814 1545 2229
Fragments arranged by size:
2814 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3254 1348
Size: 1906 4779
PciI A'CATG_T
Cuts at: 5584 5584
Size: 6685
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2600 3229 3540 3706 1108
Size: 1492 629 311 166 4087
Fragments arranged by size:
4087 1492 629 311 166
PfoI T'CCnGG_A
Cuts at: 3552 3552
Size: 6685
PmeI GTTT'AAAC
Cuts at: 3989 3989
Size: 6685
PmlI CAC'GTG
Cuts at: 3503 3503
Size: 6685
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4754 4786 6267 6299 4754
Size: 32 1481 32 5140
Fragments arranged by size:
5140 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2039 2039
Size: 6685
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6685
PsiI TTA'TAA
Cuts at: 4255 4255
Size: 6685
PspOMI G'GGCC_C
Cuts at: 3258 3388 3518 3258
Size: 130 130 6425
Fragments arranged by size:
6425 130 130
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2690 2722 3179 3211 2690
Size: 32 457 32 6164
Fragments arranged by size:
6164 457 32 32
PstI C_TGCA'G
Cuts at: 830 3876 4037 830
Size: 3046 161 3478
Fragments arranged by size:
3478 3046 161
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6685
PvuII CAG'CTG
Cuts at: 2742 2916 2742
Size: 174 6511
SacI G_AGCT'C
Cuts at: 3336 3552 3336
Size: 216 6469
SalI G'TCGA_C
Cuts at: 1936 4027 1936
Size: 2091 4594
SanDI GG'GwC_CC
Cuts at: 2039 2039
Size: 6685
SapI GCTCTTCn'nnn_
Cuts at: 3935 3935
Size: 6685
SbfI CC_TGCA'GG
Cuts at: 3876 4037 3876
Size: 161 6524
ScaI AGT'ACT
Cuts at: 2699 4933 6645 2699
Size: 2234 1712 2739
Fragments arranged by size:
2739 2234 1712
SfcI C'TryA_G
Cuts at: 826 1045 3872 4033 5168 5849 6040 826
Size: 219 2827 161 1135 681 191 1471
Fragments arranged by size:
2827 1471 1135 681 219 191 161
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6685
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6685
SmaI CCC'GGG
Cuts at: 1872 2024 4012 1872
Size: 152 1988 4545
Fragments arranged by size:
4545 1988 152
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4789 5690 5952 820
Size: 197 926 2846 901 262 1553
Fragments arranged by size:
2846 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4476 493
Size: 3983 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6685
SphI G_CATG'C
Cuts at: 1132 4043 1132
Size: 2911 3774
SspI AAT'ATT
Cuts at: 5 52 4609 5
Size: 47 4557 2081
Fragments arranged by size:
4557 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6685
StyI C'CwwG_G
Cuts at: 513 1065 2004 2187 2646 2847 3181 3228 3361
Size: 552 939 183 459 201 334 47 133
Cuts at: 3361 3513 4124 513
Size: 152 611 3074
Fragments arranged by size:
3074 939 611 552 459 334 201 183
152 133 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3286 4712 4729 4882 5067 5512 325
Size: 1255 1706 1426 17 153 185 445 1498
Fragments arranged by size:
1706 1498 1426 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2697 2733 4931
Size: 274 80 33 51 1443 720 36 2198
Cuts at: 4931 6643 96
Size: 1712 138
Fragments arranged by size:
2198 1712 1443 720 274 138 80 51
36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6645 6677 6645
Size: 32 6653
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6685
XbaI T'CTAG_A
Cuts at: 4021 4021
Size: 6685
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3382 3382
Size: 6685
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6685
XmaI C'CCGG_G
Cuts at: 1870 2022 4010 1870
Size: 152 1988 4545
Fragments arranged by size:
4545 1988 152
XmnI GAAnn'nnTTC
Cuts at: 2171 4814 2171
Size: 2643 4042
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4493 276
Size: 53 83 186 936 2959 2468
Fragments arranged by size:
2959 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI DraIII EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PspXI RsrII SacII
SexAI SfiI SfoI SrfI SwaI