Restriction map for
pFN21AB5694
(Circular) (Six-base) MAPSORT of: pFN21AB5694.seq Check: 839 from: 1 to: 6643
pFN21AB5694 6643 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3042 4453 278
Size: 53 83 186 936 1506 1411 2468
Fragments arranged by size:
2468 1506 1411 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2184 2400 3986 1937
Size: 247 216 1586 4594
Fragments arranged by size:
4594 1586 247 216
Acc65I G'GTAC_C
Cuts at: 2502 3964 2502
Size: 1462 5181
AclI AA'CG_TT
Cuts at: 1563 4770 5143 1563
Size: 3207 373 3063
Fragments arranged by size:
3207 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2162 2567 2648 2810 2833 3164 4706 6090
Size: 530 405 81 162 23 331 1542 1384
Cuts at: 6090 6358 1632
Size: 268 1917
Fragments arranged by size:
1917 1542 1384 530 405 331 268 162
81 23
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6446
AflIII A'CryG_T
Cuts at: 1203 5542 6519 1203
Size: 4339 977 1327
Fragments arranged by size:
4339 1327 977
AgeI A'CCGG_T
Cuts at: 5474 5474
Size: 6643
AhdI GACnn_n'nnGTC
Cuts at: 5372 5372
Size: 6643
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6643
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6643
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3110 3142 3110
Size: 32 6611
AlwNI CAG_nnn'CTG
Cuts at: 2971 3062 3136 3160 4386 5958 2971
Size: 91 74 24 1226 1572 3656
Fragments arranged by size:
3656 1572 1226 91 74 24
ApaLI G'TGCA_C
Cuts at: 4700 5856 4700
Size: 1156 5487
ApoI r'AATT_y
Cuts at: 1493 3952 4178 4330 6439 1493
Size: 2459 226 152 2109 1697
Fragments arranged by size:
2459 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5197 160
Size: 872 4165 1606
Fragments arranged by size:
4165 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6643
AvaI C'yCGr_G
Cuts at: 1870 1943 3122 3968 1870
Size: 73 1179 846 4545
Fragments arranged by size:
4545 1179 846 73
BamHI G'GATC_C
Cuts at: 3973 4340 3973
Size: 367 6276
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2502 3372 3964 5419 618
Size: 325 217 627 715 870 592 1455 1842
Fragments arranged by size:
1842 1455 870 715 627 592 325 217
BanII G_rGCy'C
Cuts at: 1391 2679 2778 3904 3962 1391
Size: 1288 99 1126 58 4072
Fragments arranged by size:
4072 1288 1126 99 58
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2655 928
Size: 971 756 4916
Fragments arranged by size:
4916 971 756
BbvCI CC'TCA_GC
Cuts at: 2174 3599 2174
Size: 1425 5218
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4834 4868 1903
Size: 34 2897 34 3678
Fragments arranged by size:
3678 2897 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4535 5745 4535
Size: 1210 5433
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6643
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3775 5254 136
Size: 107 122 71 631 605 2103 1479 1525
Fragments arranged by size:
2103 1525 1479 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6638 6638
Size: 6643
BlpI GC'TnA_GC
Cuts at: 2088 2279 4060 2088
Size: 191 1781 4671
Fragments arranged by size:
4671 1781 191
Bme1580I G_kGCm'C
Cuts at: 1790 2079 3240 3375 4704 5860 1790
Size: 289 1161 135 1329 1156 2573
Fragments arranged by size:
2573 1329 1161 1156 289 135
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6643
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2152 2656 2688
Size: 416 53 83 399 24 735 504 32
Cuts at: 2688 4374 5332 442
Size: 1686 958 1753
Fragments arranged by size:
1753 1686 958 735 504 416 399 83
53 32 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6643
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2270 2945 3566 5303 1239
Size: 1031 675 621 1737 2579
Fragments arranged by size:
2579 1737 1031 675 621
Bpu10I CC'TnA_GC
Cuts at: 2103 2174 3446 3599 3781 2103
Size: 71 1272 153 182 4965
Fragments arranged by size:
4965 1272 182 153 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2301 2499 4768 5633 5931 2301
Size: 198 2269 865 298 3013
Fragments arranged by size:
3013 2269 865 298 198
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3903 5306 882
Size: 617 2404 1403 2219
Fragments arranged by size:
2404 2219 1403 617
BsaAI yAC'GTr
Cuts at: 493 1204 4434 493
Size: 711 3230 2702
Fragments arranged by size:
3230 2702 711
BsaBI GATnn'nnATC
Cuts at: 6637 6637
Size: 6643
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3039 3230 4450 4832
Size: 53 83 186 936 1506 191 1220 382
Cuts at: 4832 5467 275
Size: 635 1451
Fragments arranged by size:
1506 1451 1220 936 635 382 191 186
83 53
BsaWI w'CCGG_w
Cuts at: 2410 5076 5474 5748 5895 6306 2410
Size: 2666 398 274 147 411 2747
Fragments arranged by size:
2747 2666 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3626 3656 1178
Size: 30 165 30 2223 30 4165
Fragments arranged by size:
4165 2223 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2429 2486 2567 1186
Size: 1243 57 81 5262
Fragments arranged by size:
5262 1243 81 57
BseYI C'CCAG_C
Cuts at: 2870 3239 3727 5846 6342 2870
Size: 369 488 2119 496 3171
Fragments arranged by size:
3171 2119 496 488 369
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3793 1163
Size: 573 2057 4013
Fragments arranged by size:
4013 2057 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4093 4854 5882 1540
Size: 275 186 2092 761 1028 2301
Fragments arranged by size:
2301 2092 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2679 3452 4704 4789 5860 2679
Size: 773 1252 85 1071 3462
Fragments arranged by size:
3462 1252 1071 773 85
BsmI GAATG_Cn'
Cuts at: 2790 3305 3503 4154 4247 2790
Size: 515 198 651 93 5186
Fragments arranged by size:
5186 651 515 198 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3816 3816
Size: 6643
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2679 2778 3240 3375 3452 3904
Size: 399 289 600 99 462 135 77 452
Cuts at: 3904 3962 4704 4789 5860 1391
Size: 58 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 600 462 452 399 289
135 99 85 77 58
BspEI T'CCGG_A
Cuts at: 2410 6306 2410
Size: 3896 2747
BspHI T'CATG_A
Cuts at: 4532 6262 4532
Size: 1730 4913
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3998 844
Size: 3154 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4530 1394
Size: 429 2707 3507
Fragments arranged by size:
3507 2707 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5138 5312 65
Size: 1183 3890 174 1396
Fragments arranged by size:
3890 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2538 2685 5287 5474 1700
Size: 110 728 147 2602 187 2869
Fragments arranged by size:
2869 2602 728 187 147 110
BsrGI T'GTAC_A
Cuts at: 96 2607 96
Size: 2511 4132
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6643
BssSI C'ACGA_G
Cuts at: 3464 4703 5715 3464
Size: 1239 1012 4392
Fragments arranged by size:
4392 1239 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6643
BstBI TT'CG_AA
Cuts at: 5447 5447
Size: 6643
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6643
BstXI CCAn_nnnn'nTGG
Cuts at: 3517 3517
Size: 6643
BstYI r'GATC_y
Cuts at: 1972 3430 3973 4340 4725 4742 6194 6280 6292
Size: 1458 543 367 385 17 1452 86 12
Cuts at: 6292 6638 1972
Size: 346 1977
Fragments arranged by size:
1977 1458 1452 543 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2469 2469
Size: 6643
BtgI C'CryG_G
Cuts at: 513 1065 2004 2778 513
Size: 552 939 774 4378
Fragments arranged by size:
4378 939 774 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5904
Fragments arranged by size:
5904 683 56
BtsI GCAGTG_nn'
Cuts at: 3609 4159 4953 4973 3609
Size: 550 794 20 5279
Fragments arranged by size:
5279 794 550 20
ClaI AT'CG_AT
Cuts at: 1554 3930 1554
Size: 2376 4267
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6608
DraI TTT'AAA
Cuts at: 3947 3947
Size: 6643
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2593 2912 5650 809
Size: 507 1277 319 2738 1802
Fragments arranged by size:
2738 1802 1277 507 319
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2536 3279 4090
Size: 54 1425 50 275 18 706 743 811
Cuts at: 4090 4979 6608 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 811 743 706 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4090 1537
Size: 275 2278 4090
Fragments arranged by size:
4090 2278 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2886 3138 3545 3855 4573 1353
Size: 1533 252 407 310 718 3423
Fragments arranged by size:
3423 1533 718 407 310 252
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5230 5604 5750 387
Size: 1064 138 3641 374 146 1280
Fragments arranged by size:
3641 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2677 2677
Size: 6643
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2162 2270 2567 2648 2810 2833 2945
Size: 393 530 108 297 81 162 23 112
Cuts at: 2945 3164 3566 4706 5303 6090 6358 1239
Size: 219 402 1140 597 787 268 1524
Fragments arranged by size:
1524 1140 787 597 530 402 393 297
268 219 162 112 108 81 23
EcoNI CCTnn'n_nnAGG
Cuts at: 3875 3875
Size: 6643
EcoO109I rG'GnC_Cy
Cuts at: 2914 2968 3118 3170 2914
Size: 54 150 52 6387
Fragments arranged by size:
6387 150 54 52
EcoRI G'AATT_C
Cuts at: 3952 4330 3952
Size: 378 6265
EcoRV GAT'ATC
Cuts at: 2991 2991
Size: 6643
FseI GG_CCGG'CC
Cuts at: 2542 2542
Size: 6643
FspI TGC'GCA
Cuts at: 5149 5149
Size: 6643
HaeII r_GCGC'y
Cuts at: 1678 2365 2956 3735 3781 5790 1678
Size: 687 591 779 46 2009 2531
Fragments arranged by size:
2531 2009 779 687 591 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5284 5316 5358 5390 1373
Size: 32 135 32 3712 32 42 32 2626
Fragments arranged by size:
3712 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2185 2434 3274 3403 3987 4233
Size: 988 281 247 249 840 129 584 246
Cuts at: 4233 669
Size: 3079
Fragments arranged by size:
3079 988 840 584 281 249 247 246
129
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6643
HpaI GTT'AAC
Cuts at: 4233 4233
Size: 6643
KpnI G_GTAC'C
Cuts at: 2506 3968 2506
Size: 1462 5181
MfeI C'AATT_G
Cuts at: 4242 4242
Size: 6643
MluI A'CGCG_T
Cuts at: 6519 6519
Size: 6643
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3462 3534 3606 5757 5941 1305
Size: 2157 72 72 2151 184 2007
Fragments arranged by size:
2157 2151 2007 184 72 72
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3281 10
Size: 54 1425 343 1449 3372
Fragments arranged by size:
3372 1449 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2450 4601 4960 5119 518
Size: 648 1284 2151 359 159 2042
Fragments arranged by size:
2151 2042 1284 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2275 2638 3268 3888 4059 4736 5884 6129
Size: 669 363 630 620 171 677 1148 245
Cuts at: 6129 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 669 630 620 363 245
171
NaeI GCC'GGC
Cuts at: 2540 2540
Size: 6643
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5152
Fragments arranged by size:
5152 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6643
NgoMIV G'CCGG_C
Cuts at: 2538 2538
Size: 6643
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6643
NotI GC'GGCC_GC
Cuts at: 4090 4090
Size: 6643
NspI r_CATG'y
Cuts at: 1132 1229 3944 4001 5546 1132
Size: 97 2715 57 1545 2229
Fragments arranged by size:
2715 2229 1545 97 57
PasI CC'CwG_GG
Cuts at: 1348 2440 1348
Size: 1092 5551
PciI A'CATG_T
Cuts at: 5542 5542
Size: 6643
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2590 3160 3393 1108
Size: 1482 570 233 4358
Fragments arranged by size:
4358 1482 570 233
PfoI T'CCnGG_A
Cuts at: 2286 3392 3543 3904 2286
Size: 1106 151 361 5025
Fragments arranged by size:
5025 1106 361 151
PmeI GTTT'AAAC
Cuts at: 3947 3947
Size: 6643
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4712 4744 6225 6257 4712
Size: 32 1481 32 5098
Fragments arranged by size:
5098 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2914 3118 2914
Size: 204 6439
PshAI GACnn'nnGTC
Cuts at: 1613 2318 1613
Size: 705 5938
PsiI TTA'TAA
Cuts at: 4213 4213
Size: 6643
PspXI vC'TCGA_Gb
Cuts at: 3122 3122
Size: 6643
PstI C_TGCA'G
Cuts at: 830 3879 3995 830
Size: 3049 116 3478
Fragments arranged by size:
3478 3049 116
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6643
PvuII CAG'CTG
Cuts at: 2638 3268 2638
Size: 630 6013
SacI G_AGCT'C
Cuts at: 2679 2679
Size: 6643
SalI G'TCGA_C
Cuts at: 1936 2183 3985 1936
Size: 247 1802 4594
Fragments arranged by size:
4594 1802 247
SbfI CC_TGCA'GG
Cuts at: 3879 3995 3879
Size: 116 6527
ScaI AGT'ACT
Cuts at: 4891 6603 4891
Size: 1712 4931
SfcI C'TryA_G
Cuts at: 826 1045 3875 3991 5126 5807 5998 826
Size: 219 2830 116 1135 681 191 1471
Fragments arranged by size:
2830 1471 1135 681 219 191 116
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6643
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6643
SmaI CCC'GGG
Cuts at: 1872 3970 1872
Size: 2098 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2316 2514 3122 4747 5648 5910
Size: 197 926 373 198 608 1625 901 262
Cuts at: 5910 820
Size: 1553
Fragments arranged by size:
1625 1553 926 901 608 373 262 198
197
SnaBI TAC'GTA
Cuts at: 493 4434 493
Size: 3941 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6643
SphI G_CATG'C
Cuts at: 1132 4001 1132
Size: 2869 3774
SspI AAT'ATT
Cuts at: 5 52 4567 5
Size: 47 4515 2081
Fragments arranged by size:
4515 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6643
StyI C'CwwG_G
Cuts at: 513 1065 2004 2164 3296 4082 513
Size: 552 939 160 1132 786 3074
Fragments arranged by size:
3074 1132 939 786 552 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2358 3316 4670 4687 4840 5025 5470
Size: 1255 778 958 1354 17 153 185 445
Cuts at: 5470 325
Size: 1498
Fragments arranged by size:
1498 1354 1255 958 778 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2607 4889 6601
Size: 274 80 33 51 1443 630 2282 1712
Cuts at: 6601 96
Size: 138
Fragments arranged by size:
2282 1712 1443 630 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2801 2833 3426 3458 3601 3633 6603 6635 2801
Size: 32 593 32 143 32 2970 32 2809
Fragments arranged by size:
2970 2809 593 143 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6643
XbaI T'CTAG_A
Cuts at: 3979 3979
Size: 6643
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2255 2255
Size: 6643
XhoI C'TCGA_G
Cuts at: 1943 3122 1943
Size: 1179 5464
XmaI C'CCGG_G
Cuts at: 1870 3968 1870
Size: 2098 4545
XmnI GAAnn'nnTTC
Cuts at: 4772 4772
Size: 6643
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3040 4451 276
Size: 53 83 186 936 1506 1411 2468
Fragments arranged by size:
2468 1506 1411 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I DraIII FalI FspAI KasI NarI
NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI