Restriction map for
pFN21AB5686
(Circular) (Six-base) MAPSORT of: pFN21AB5686.seq Check: 5528 from: 1 to: 6145
pFN21AB5686 6145 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3955 278
Size: 53 83 186 936 2419 2468
Fragments arranged by size:
2468 2419 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3488 1937
Size: 1551 4594
Acc65I G'GTAC_C
Cuts at: 3466 3466
Size: 6145
AclI AA'CG_TT
Cuts at: 1563 4272 4645 1563
Size: 2709 373 3063
Fragments arranged by size:
3063 2709 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4208 5592 5860 1632
Size: 2576 1384 268 1917
Fragments arranged by size:
2576 1917 1384 268
AfeI AGC'GCT
Cuts at: 2181 2717 2181
Size: 536 5609
AflII C'TTAA_G
Cuts at: 820 1017 3425 820
Size: 197 2408 3540
Fragments arranged by size:
3540 2408 197
AflIII A'CryG_T
Cuts at: 1203 2706 2708 2825 5044 6021 1203
Size: 1503 2 117 2219 977 1327
Fragments arranged by size:
2219 1503 1327 977 117 2
AgeI A'CCGG_T
Cuts at: 4976 4976
Size: 6145
AhdI GACnn_n'nnGTC
Cuts at: 2201 4874 2201
Size: 2673 3472
AleI CACnn'nnGTG
Cuts at: 1166 2259 2750 2769 1166
Size: 1093 491 19 4542
Fragments arranged by size:
4542 1093 491 19
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2484 2768 1184
Size: 1300 284 4561
Fragments arranged by size:
4561 1300 284
AlwNI CAG_nnn'CTG
Cuts at: 3106 3271 3888 5460 3106
Size: 165 617 1572 3791
Fragments arranged by size:
3791 1572 617 165
ApaI G_GGCC'C
Cuts at: 2086 2086
Size: 6145
ApaLI G'TGCA_C
Cuts at: 4202 5358 4202
Size: 1156 4989
ApoI r'AATT_y
Cuts at: 1493 2982 3454 3680 3832 5941 1493
Size: 1489 472 226 152 2109 1697
Fragments arranged by size:
2109 1697 1489 472 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4699 160
Size: 872 3667 1606
Fragments arranged by size:
3667 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
AvaI C'yCGr_G
Cuts at: 1870 1943 2175 2549 3470 1870
Size: 73 232 374 921 4545
Fragments arranged by size:
4545 921 374 232 73
BamHI G'GATC_C
Cuts at: 3475 3842 3475
Size: 367 5778
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2744 3466 4921 618
Size: 325 217 627 957 722 1455 1842
Fragments arranged by size:
1842 1455 957 722 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2086 2742 3100 3464 1391
Size: 695 656 358 364 4072
Fragments arranged by size:
4072 695 656 364 358
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2145 928
Size: 971 246 4928
Fragments arranged by size:
4928 971 246
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4336 4370 1903
Size: 34 2399 34 3678
Fragments arranged by size:
3678 2399 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4037 5247 4037
Size: 1210 4935
BclI T'GATC_A
Cuts at: 1548 2620 1548
Size: 1072 5073
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2270 4756 136
Size: 107 122 71 631 605 598 2486 1525
Fragments arranged by size:
2486 1525 631 605 598 122 107 71
BglII A'GATC_T
Cuts at: 2568 6140 2568
Size: 3572 2573
BlpI GC'TnA_GC
Cuts at: 2120 2132 3562 2120
Size: 12 1430 4703
Fragments arranged by size:
4703 1430 12
Bme1580I G_kGCm'C
Cuts at: 1790 2086 2266 4206 5362 1790
Size: 296 180 1940 1156 2573
Fragments arranged by size:
2573 1940 1156 296 180
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6145
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3876 4834 442
Size: 416 53 83 399 24 2459 958 1753
Fragments arranged by size:
2459 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6145
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2425 2425
Size: 6145
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4805 1239
Size: 3566 2579
Bpu10I CC'TnA_GC
Cuts at: 3069 3278 3069
Size: 209 5936
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4270 5135 5433 4270
Size: 865 298 4982
Fragments arranged by size:
4982 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2188 3204 4808 882
Size: 617 689 1016 1604 2219
Fragments arranged by size:
2219 1604 1016 689 617
BsaAI yAC'GTr
Cuts at: 493 1204 2709 2826 3936 493
Size: 711 1505 117 1110 2702
Fragments arranged by size:
2702 1505 1110 711 117
BsaBI GATnn'nnATC
Cuts at: 2286 6139 2286
Size: 3853 2292
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3324 3952 4334 4969
Size: 53 83 186 936 1791 628 382 635
Cuts at: 4969 275
Size: 1451
Fragments arranged by size:
1791 1451 936 635 628 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3021 4578 4976 5250 5397 5808 3021
Size: 1557 398 274 147 411 3358
Fragments arranged by size:
3358 1557 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2824 2854 3029 3059 1178
Size: 30 165 30 1421 30 175 30 4264
Fragments arranged by size:
4264 1421 175 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2567 3419 1186
Size: 1381 852 3912
Fragments arranged by size:
3912 1381 852
BseYI C'CCAG_C
Cuts at: 2412 2511 3392 5348 5844 2412
Size: 99 881 1956 496 2713
Fragments arranged by size:
2713 1956 881 496 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2774 3023 1163
Size: 573 1038 249 4285
Fragments arranged by size:
4285 1038 573 249
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2090 3595 4356 5384 1540
Size: 275 186 89 1505 761 1028 2301
Fragments arranged by size:
2301 1505 1028 761 275 186 89
BsiHKAI G_wGCw'C
Cuts at: 2021 4206 4291 5362 2021
Size: 2185 85 1071 2804
Fragments arranged by size:
2804 2185 1071 85
BsmI GAATG_Cn'
Cuts at: 3656 3749 3656
Size: 93 6052
BsmBI CGTCTCn'nnnn_
Cuts at: 3107 3107
Size: 6145
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2086 2266 2742 3100 3464 4206
Size: 399 231 65 180 476 358 364 742
Cuts at: 4206 4291 5362 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 476 399 364 358 231
180 85 65
BspEI T'CCGG_A
Cuts at: 3021 5808 3021
Size: 2787 3358
BspHI T'CATG_A
Cuts at: 4034 5764 4034
Size: 1730 4415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3500 844
Size: 2656 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4032 1394
Size: 429 2209 3507
Fragments arranged by size:
3507 2209 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2758 4640 4814 65
Size: 1183 1510 1882 174 1396
Fragments arranged by size:
1882 1510 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2062 3258 4789 4976 1700
Size: 110 252 1196 1531 187 2869
Fragments arranged by size:
2869 1531 1196 252 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6145
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6145
BssSI C'ACGA_G
Cuts at: 2257 4205 5217 2257
Size: 1948 1012 3185
Fragments arranged by size:
3185 1948 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6145
BstBI TT'CG_AA
Cuts at: 4949 4949
Size: 6145
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6145
BstXI CCAn_nnnn'nTGG
Cuts at: 2771 2771
Size: 6145
BstYI r'GATC_y
Cuts at: 1972 2568 3475 3842 4227 4244 5696 5782 5794
Size: 596 907 367 385 17 1452 86 12
Cuts at: 5794 6140 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 907 596 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2628 2628
Size: 6145
BtgI C'CryG_G
Cuts at: 513 1065 2004 2054 2845 3354 513
Size: 552 939 50 791 509 3304
Fragments arranged by size:
3304 939 791 552 509 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5406
Fragments arranged by size:
5406 683 56
BtsI GCAGTG_nn'
Cuts at: 3661 4455 4475 3661
Size: 794 20 5331
Fragments arranged by size:
5331 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6145
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6110
DraI TTT'AAA
Cuts at: 3449 3449
Size: 6145
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5152 809
Size: 507 3836 1802
Fragments arranged by size:
3836 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2087 2271 2842
Size: 54 1425 50 275 18 257 184 571
Cuts at: 2842 2947 3592 4481 6110 8
Size: 105 645 889 1629 43
Fragments arranged by size:
1629 1425 889 645 571 275 257 184
105 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2087 3592 1537
Size: 275 275 1505 4090
Fragments arranged by size:
4090 1505 275 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2447 2596 3081 4075 1353
Size: 1094 149 485 994 3423
Fragments arranged by size:
3423 1094 994 485 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4732 5106 5252 387
Size: 1064 138 3143 374 146 1280
Fragments arranged by size:
3143 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4208 4805 5592 5860 1239
Size: 393 2576 597 787 268 1524
Fragments arranged by size:
2576 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2049 2082 2534 2804 3103 2049
Size: 33 452 270 299 5091
Fragments arranged by size:
5091 452 299 270 33
EcoRI G'AATT_C
Cuts at: 3454 3832 3454
Size: 378 5767
FspI TGC'GCA
Cuts at: 4651 4651
Size: 6145
HaeII r_GCGC'y
Cuts at: 1678 2183 2719 5292 1678
Size: 505 536 2573 2531
Fragments arranged by size:
2573 2531 536 505
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2187 2219 2570 2602 2641
Size: 32 135 32 615 32 351 32 39
Cuts at: 2641 2673 2904 2936 3029 3061 4786 4818 4860
Size: 32 231 32 93 32 1725 32 42
Cuts at: 4860 4892 1373
Size: 32 2626
Fragments arranged by size:
2626 1725 615 351 231 135 93 42
39 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2649 2677 3489 3735 669
Size: 988 281 711 28 812 246 3079
Fragments arranged by size:
3079 988 812 711 281 246 28
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6145
HpaI GTT'AAC
Cuts at: 3735 3735
Size: 6145
KpnI G_GTAC'C
Cuts at: 3470 3470
Size: 6145
MfeI C'AATT_G
Cuts at: 3744 3744
Size: 6145
MluI A'CGCG_T
Cuts at: 6021 6021
Size: 6145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3072 5259 5443 1305
Size: 1767 2187 184 2007
Fragments arranged by size:
2187 2007 1767 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2273 2949 10
Size: 54 1425 343 441 676 3206
Fragments arranged by size:
3206 1425 676 441 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2259 2750 2769 4103 4462 4621 518
Size: 648 1093 491 19 1334 359 159 2042
Fragments arranged by size:
2042 1334 1093 648 491 359 159 19
MspA1I CmG'CkG
Cuts at: 1606 2056 2416 2505 2847 3561 4238 5386 5631
Size: 450 360 89 342 714 677 1148 245
Cuts at: 5631 1606
Size: 2120
Fragments arranged by size:
2120 1148 714 677 450 360 342 245
89
NaeI GCC'GGC
Cuts at: 2064 3260 2064
Size: 1196 4949
NcoI C'CATG_G
Cuts at: 513 1065 2004 3354 513
Size: 552 939 1350 3304
Fragments arranged by size:
3304 1350 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6145
NgoMIV G'CCGG_C
Cuts at: 2062 3258 2062
Size: 1196 4949
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6145
NotI GC'GGCC_GC
Cuts at: 3592 3592
Size: 6145
NspI r_CATG'y
Cuts at: 1132 1229 3503 5048 1132
Size: 97 2274 1545 2229
Fragments arranged by size:
2274 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2094 3100 3137 1348
Size: 746 1006 37 4356
Fragments arranged by size:
4356 1006 746 37
PciI A'CATG_T
Cuts at: 5044 5044
Size: 6145
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2028 1108
Size: 920 5225
PmeI GTTT'AAAC
Cuts at: 3449 3449
Size: 6145
PmlI CAC'GTG
Cuts at: 2709 2826 2709
Size: 117 6028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2543 2575 4214 4246 5727 5759 2543
Size: 32 1639 32 1481 32 2929
Fragments arranged by size:
2929 1639 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2049 2049
Size: 6145
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6145
PsiI TTA'TAA
Cuts at: 3715 3715
Size: 6145
PspOMI G'GGCC_C
Cuts at: 2082 2082
Size: 6145
PstI C_TGCA'G
Cuts at: 830 2485 2882 2935 3497 830
Size: 1655 397 53 562 3478
Fragments arranged by size:
3478 1655 562 397 53
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6145
PvuII CAG'CTG
Cuts at: 2416 2416
Size: 6145
SacII CC_GC'GG
Cuts at: 2057 2848 2057
Size: 791 5354
SalI G'TCGA_C
Cuts at: 1936 3487 1936
Size: 1551 4594
SanDI GG'GwC_CC
Cuts at: 2049 2049
Size: 6145
SapI GCTCTTCn'nnn_
Cuts at: 3081 3081
Size: 6145
SbfI CC_TGCA'GG
Cuts at: 2935 3497 2935
Size: 562 5583
ScaI AGT'ACT
Cuts at: 4393 6105 4393
Size: 1712 4433
SexAI A'CCwGG_T
Cuts at: 2197 2197
Size: 6145
SfcI C'TryA_G
Cuts at: 826 1045 2481 2878 2931 3063 3493 4628 5309
Size: 219 1436 397 53 132 430 1135 681
Cuts at: 5309 5500 826
Size: 191 1471
Fragments arranged by size:
1471 1436 1135 681 430 397 219 191
132 53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6145
SmaI CCC'GGG
Cuts at: 1872 3472 1872
Size: 1600 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3425 4249 5150 5412 820
Size: 197 926 1482 824 901 262 1553
Fragments arranged by size:
1553 1482 926 901 824 262 197
SnaBI TAC'GTA
Cuts at: 493 3936 493
Size: 3443 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6145
SphI G_CATG'C
Cuts at: 1132 3503 1132
Size: 2371 3774
SspI AAT'ATT
Cuts at: 5 52 4069 5
Size: 47 4017 2081
Fragments arranged by size:
4017 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6145
StyI C'CwwG_G
Cuts at: 513 1065 2004 2397 2748 3354 3584 513
Size: 552 939 393 351 606 230 3074
Fragments arranged by size:
3074 939 606 552 393 351 230
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3158 4172 4189 4342 4527 4972 325
Size: 1255 1578 1014 17 153 185 445 1498
Fragments arranged by size:
1578 1498 1255 1014 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2240 4391 6103
Size: 274 80 33 51 1443 263 2151 1712
Cuts at: 6103 96
Size: 138
Fragments arranged by size:
2151 1712 1443 274 263 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2824 2856 6105 6137 2824
Size: 32 3249 32 2832
Fragments arranged by size:
3249 2832 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6145
XbaI T'CTAG_A
Cuts at: 3481 3481
Size: 6145
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2101 2101
Size: 6145
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6145
XmaI C'CCGG_G
Cuts at: 1870 3470 1870
Size: 1600 4545
XmnI GAAnn'nnTTC
Cuts at: 4274 4274
Size: 6145
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3953 276
Size: 53 83 186 936 2419 2468
Fragments arranged by size:
2468 2419 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PfoI PspXI
PsrI RsrII SacI SfiI SfoI SrfI SwaI