Restriction map for
pFN21AB5684
(Circular) (Six-base) MAPSORT of: pFN21AB5684.seq Check: 8961 from: 1 to: 6391
pFN21AB5684 6391 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2300 2906 2300
Size: 606 5785
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4201 278
Size: 53 83 186 936 2665 2468
Fragments arranged by size:
2665 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3734 1937
Size: 1797 4594
Acc65I G'GTAC_C
Cuts at: 3712 3712
Size: 6391
AclI AA'CG_TT
Cuts at: 1563 4518 4891 1563
Size: 2955 373 3063
Fragments arranged by size:
3063 2955 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2867 3413 4454 5838 6106 1632
Size: 1235 546 1041 1384 268 1917
Fragments arranged by size:
1917 1384 1235 1041 546 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6194
AflIII A'CryG_T
Cuts at: 1203 5290 6267 1203
Size: 4087 977 1327
Fragments arranged by size:
4087 1327 977
AgeI A'CCGG_T
Cuts at: 3688 5222 3688
Size: 1534 4857
AhdI GACnn_n'nnGTC
Cuts at: 5120 5120
Size: 6391
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6391
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6391
AlwNI CAG_nnn'CTG
Cuts at: 2249 2305 2646 2779 3051 3429 4134 5706 2249
Size: 56 341 133 272 378 705 1572 2934
Fragments arranged by size:
2934 1572 705 378 341 272 133 56
ApaI G_GGCC'C
Cuts at: 3507 3507
Size: 6391
ApaLI G'TGCA_C
Cuts at: 2446 4448 5604 2446
Size: 2002 1156 3233
Fragments arranged by size:
3233 2002 1156
ApoI r'AATT_y
Cuts at: 1493 2722 3161 3194 3700 3926 4078 6187 1493
Size: 1229 439 33 506 226 152 2109 1697
Fragments arranged by size:
2109 1697 1229 506 439 226 152 33
AseI AT'TA_AT
Cuts at: 160 1032 4945 160
Size: 872 3913 1606
Fragments arranged by size:
3913 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6391
AvaI C'yCGr_G
Cuts at: 1870 1943 3631 3716 1870
Size: 73 1688 85 4545
Fragments arranged by size:
4545 1688 85 73
BamHI G'GATC_C
Cuts at: 3721 4088 3721
Size: 367 6024
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2288 2438 2702 2835 3712
Size: 325 217 627 501 150 264 133 877
Cuts at: 3712 5167 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 877 627 501 325 264 217
150 133
BanII G_rGCy'C
Cuts at: 1391 2028 2949 3342 3507 3710 1391
Size: 637 921 393 165 203 4072
Fragments arranged by size:
4072 921 637 393 203 165
BbeI G_GCGC'C
Cuts at: 2442 2442
Size: 6391
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2050 928
Size: 971 151 5269
Fragments arranged by size:
5269 971 151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2327 2361 2684 2718 4582 4616 1903
Size: 34 390 34 323 34 1864 34 3678
Fragments arranged by size:
3678 1864 390 323 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4283 5493 4283
Size: 1210 5181
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6391
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5002 136
Size: 107 122 71 631 605 3330 1525
Fragments arranged by size:
3330 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2108 6386 2108
Size: 4278 2113
BlpI GC'TnA_GC
Cuts at: 3808 3808
Size: 6391
Bme1580I G_kGCm'C
Cuts at: 1790 2233 2291 2336 2417 2450 2773 2886 3265
Size: 443 58 45 81 33 323 113 379
Cuts at: 3265 3507 4452 5608 1790
Size: 242 945 1156 2573
Fragments arranged by size:
2573 1156 945 443 379 323 242 113
81 58 45 33
BmgBI CAC'GTC
Cuts at: 1318 2865 1318
Size: 1547 4844
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2760 2789 4122
Size: 416 53 83 399 24 1343 29 1333
Cuts at: 4122 5080 442
Size: 958 1753
Fragments arranged by size:
1753 1343 1333 958 416 399 83 53
29 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6391
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3504 5051 1239
Size: 2265 1547 2579
Fragments arranged by size:
2579 2265 1547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4516 5381 5679 4516
Size: 865 298 5228
Fragments arranged by size:
5228 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5054 882
Size: 617 3555 2219
Fragments arranged by size:
3555 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2382 2418 2842 4182 493
Size: 711 1178 36 424 1340 2702
Fragments arranged by size:
2702 1340 1178 711 424 36
BsaBI GATnn'nnATC
Cuts at: 6385 6385
Size: 6391
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2439 3525 4198 4580
Size: 53 83 186 936 906 1086 673 382
Cuts at: 4580 5215 275
Size: 635 1451
Fragments arranged by size:
1451 1086 936 906 673 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3688 4824 5222 5496 5643 6054 3688
Size: 1136 398 274 147 411 4025
Fragments arranged by size:
4025 1136 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2948 2978 1178
Size: 30 165 30 1545 30 4591
Fragments arranged by size:
4591 1545 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2115 3164 3167 1186
Size: 929 1049 3 4410
Fragments arranged by size:
4410 1049 929 3
BseYI C'CCAG_C
Cuts at: 2069 2348 2515 3600 5594 6090 2069
Size: 279 167 1085 1994 496 2370
Fragments arranged by size:
2370 1994 1085 496 279 167
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3415 1163
Size: 573 1679 4139
Fragments arranged by size:
4139 1679 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3689 3841 4602 5630 1540
Size: 275 186 1688 152 761 1028 2301
Fragments arranged by size:
2301 1688 1028 761 275 186 152
BsiHKAI G_wGCw'C
Cuts at: 2187 2426 2450 3342 4452 4537 5608 2187
Size: 239 24 892 1110 85 1071 2970
Fragments arranged by size:
2970 1110 1071 892 239 85 24
BsmI GAATG_Cn'
Cuts at: 3902 3995 3902
Size: 93 6298
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2187 2233 2291 2336 2417 2426
Size: 399 238 159 46 58 45 81 9
Cuts at: 2426 2450 2773 2886 2949 3265 3342 3507 3710
Size: 24 323 113 63 316 77 165 203
Cuts at: 3710 4452 4537 5608 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 323 316 238 203
165 159 113 85 81 77 63 58
46 45 24 9
BspEI T'CCGG_A
Cuts at: 6054 6054
Size: 6391
BspHI T'CATG_A
Cuts at: 4280 6010 4280
Size: 1730 4661
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2300 2906 3318 3746 844
Size: 1456 606 412 428 3489
Fragments arranged by size:
3489 1456 606 428 412
BsrBI CCG'CTC
Cuts at: 1394 1823 3019 3388 3498 4278 1394
Size: 429 1196 369 110 780 3507
Fragments arranged by size:
3507 1196 780 429 369 110
BsrDI GCAATG_nn'
Cuts at: 65 1248 2019 3222 4886 5060 65
Size: 1183 771 1203 1664 174 1396
Fragments arranged by size:
1664 1396 1203 1183 771 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3688 5035 5222 1700
Size: 110 1878 1347 187 2869
Fragments arranged by size:
2869 1878 1347 187 110
BsrGI T'GTAC_A
Cuts at: 96 3639 96
Size: 3543 2848
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6391
BssSI C'ACGA_G
Cuts at: 4451 5463 4451
Size: 1012 5379
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3291 794
Size: 2497 3894
BstBI TT'CG_AA
Cuts at: 5195 5195
Size: 6391
BstEII G'GTnAC_C
Cuts at: 1184 3649 1184
Size: 2465 3926
BstXI CCAn_nnnn'nTGG
Cuts at: 2515 2515
Size: 6391
BstYI r'GATC_y
Cuts at: 1972 2108 3721 4088 4473 4490 5942 6028 6040
Size: 136 1613 367 385 17 1452 86 12
Cuts at: 6040 6386 1972
Size: 346 1977
Fragments arranged by size:
1977 1613 1452 385 367 346 136 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2674 2717 513
Size: 552 939 670 43 4187
Fragments arranged by size:
4187 939 670 552 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2944 487
Size: 683 56 1718 3934
Fragments arranged by size:
3934 1718 683 56
BtsI GCAGTG_nn'
Cuts at: 3073 3907 4701 4721 3073
Size: 834 794 20 4743
Fragments arranged by size:
4743 834 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6391
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6356
DraI TTT'AAA
Cuts at: 3695 3695
Size: 6391
DraIII CAC_nnn'GTG
Cuts at: 2245 3291 2245
Size: 1046 5345
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5398 809
Size: 507 4082 1802
Fragments arranged by size:
4082 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3571 3838 4727
Size: 54 1425 50 275 18 1741 267 889
Cuts at: 4727 6356 8
Size: 1629 43
Fragments arranged by size:
1741 1629 1425 889 275 267 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3838 1537
Size: 275 2026 4090
Fragments arranged by size:
4090 2026 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2012 2092 2954 4321 1353
Size: 659 80 862 1367 3423
Fragments arranged by size:
3423 1367 862 659 80
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2509 3084 3320 4978 5352 5498
Size: 1064 138 920 575 236 1658 374 146
Cuts at: 5498 387
Size: 1280
Fragments arranged by size:
1658 1280 1064 920 575 374 236 146
138
EcoICRI GAG'CTC
Cuts at: 3340 3340
Size: 6391
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2867 3413 3504 4454 5051 5838 6106
Size: 393 1235 546 91 950 597 787 268
Cuts at: 6106 1239
Size: 1524
Fragments arranged by size:
1524 1235 950 787 597 546 393 268
91
EcoO109I rG'GnC_Cy
Cuts at: 2302 3504 3548 3604 2302
Size: 1202 44 56 5089
Fragments arranged by size:
5089 1202 56 44
EcoRI G'AATT_C
Cuts at: 2722 3194 3700 4078 2722
Size: 472 506 378 5035
Fragments arranged by size:
5035 506 472 378
FspI TGC'GCA
Cuts at: 4897 4897
Size: 6391
HaeII r_GCGC'y
Cuts at: 1678 2442 5538 1678
Size: 764 3096 2531
Fragments arranged by size:
3096 2531 764
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2543 2575 2870 2902 2948
Size: 32 135 32 971 32 295 32 46
Cuts at: 2948 2980 3353 3385 5032 5064 5106 5138 1373
Size: 32 373 32 1647 32 42 32 2626
Fragments arranged by size:
2626 1647 971 373 295 135 46 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3735 3981 669
Size: 988 281 1797 246 3079
Fragments arranged by size:
3079 1797 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6391
HpaI GTT'AAC
Cuts at: 3981 3981
Size: 6391
KasI G'GCGC_C
Cuts at: 2438 2438
Size: 6391
KpnI G_GTAC'C
Cuts at: 3716 3716
Size: 6391
MfeI C'AATT_G
Cuts at: 3990 3990
Size: 6391
MluI A'CGCG_T
Cuts at: 6267 6267
Size: 6391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5505 5689 1305
Size: 4200 184 2007
Fragments arranged by size:
4200 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4569
Fragments arranged by size:
4569 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4349 4708 4867 518
Size: 648 3183 359 159 2042
Fragments arranged by size:
3183 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2429 2490 3238 3258 3316 3429 3600 3807
Size: 823 61 748 20 58 113 171 207
Cuts at: 3807 4484 5632 5877 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 823 748 677 245 207 171
113 61 58 20
NarI GG'CG_CC
Cuts at: 2439 2439
Size: 6391
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4900
Fragments arranged by size:
4900 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6391
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6391
NotI GC'GGCC_GC
Cuts at: 3838 3838
Size: 6391
NspI r_CATG'y
Cuts at: 1132 1229 3475 3685 3749 5294 1132
Size: 97 2246 210 64 1545 2229
Fragments arranged by size:
2246 2229 1545 210 97 64
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6391
PciI A'CATG_T
Cuts at: 5290 5290
Size: 6391
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2779 2832 1108
Size: 1671 53 4667
Fragments arranged by size:
4667 1671 53
PfoI T'CCnGG_A
Cuts at: 3081 3081
Size: 6391
PmeI GTTT'AAAC
Cuts at: 3695 3695
Size: 6391
PmlI CAC'GTG
Cuts at: 2382 2418 2842 2382
Size: 36 424 5931
Fragments arranged by size:
5931 424 36
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4460 4492 5973 6005 4460
Size: 32 1481 32 4846
Fragments arranged by size:
4846 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6391
PsiI TTA'TAA
Cuts at: 3961 3961
Size: 6391
PspOMI G'GGCC_C
Cuts at: 3503 3503
Size: 6391
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2519 2551 2519
Size: 32 6359
PstI C_TGCA'G
Cuts at: 830 2129 2543 2615 3237 3315 3743 830
Size: 1299 414 72 622 78 428 3478
Fragments arranged by size:
3478 1299 622 428 414 78 72
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6391
PvuII CAG'CTG
Cuts at: 3238 3316 3600 3238
Size: 78 284 6029
Fragments arranged by size:
6029 284 78
SacI G_AGCT'C
Cuts at: 3342 3342
Size: 6391
SalI G'TCGA_C
Cuts at: 1936 3733 1936
Size: 1797 4594
SapI GCTCTTCn'nnn_
Cuts at: 2954 2954
Size: 6391
SbfI CC_TGCA'GG
Cuts at: 3743 3743
Size: 6391
ScaI AGT'ACT
Cuts at: 2873 4639 6351 2873
Size: 1766 1712 2913
Fragments arranged by size:
2913 1766 1712
SfcI C'TryA_G
Cuts at: 826 1045 2125 2407 2485 2539 2611 3233 3311
Size: 219 1080 282 78 54 72 622 78
Cuts at: 3311 3739 4874 5555 5746 826
Size: 428 1135 681 191 1471
Fragments arranged by size:
1471 1135 1080 681 622 428 282 219
191 78 78 72 54
SfoI GGC'GCC
Cuts at: 2440 2440
Size: 6391
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6391
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6391
SmaI CCC'GGG
Cuts at: 1872 3633 3718 1872
Size: 1761 85 4545
Fragments arranged by size:
4545 1761 85
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4495 5396 5658 820
Size: 197 926 2552 901 262 1553
Fragments arranged by size:
2552 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4182 493
Size: 3689 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6391
SphI G_CATG'C
Cuts at: 1132 3749 1132
Size: 2617 3774
SspI AAT'ATT
Cuts at: 5 52 2207 4315 5
Size: 47 2155 2108 2081
Fragments arranged by size:
2155 2108 2081 47
StuI AGG'CCT
Cuts at: 1517 3365 1517
Size: 1848 4543
StyI C'CwwG_G
Cuts at: 513 1065 2004 2270 3645 3830 513
Size: 552 939 266 1375 185 3074
Fragments arranged by size:
3074 1375 939 552 266 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4418 4435 4588 4773 5218 325
Size: 1255 2838 17 153 185 445 1498
Fragments arranged by size:
2838 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2307 2595 2871
Size: 274 80 33 51 1443 330 288 276
Cuts at: 2871 2913 3639 4637 6349 96
Size: 42 726 998 1712 138
Fragments arranged by size:
1712 1443 998 726 330 288 276 274
138 80 51 42 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2344 2376 3319 3351 6351 6383 2344
Size: 32 943 32 3000 32 2352
Fragments arranged by size:
3000 2352 943 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6391
XbaI T'CTAG_A
Cuts at: 3727 3727
Size: 6391
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6391
XmaI C'CCGG_G
Cuts at: 1870 3631 3716 1870
Size: 1761 85 4545
Fragments arranged by size:
4545 1761 85
XmnI GAAnn'nnTTC
Cuts at: 4520 4520
Size: 6391
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4199 276
Size: 53 83 186 936 2665 2468
Fragments arranged by size:
2665 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI
PspXI RsrII SacII SanDI SexAI SfiI SrfI SwaI
XcmI