Restriction map for
pFN21AB5680
(Circular) (Six-base) MAPSORT of: pFN21AB5680.seq Check: 3119 from: 1 to: 6145
pFN21AB5680 6145 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3955 278
Size: 53 83 186 936 2419 2468
Fragments arranged by size:
2468 2419 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3488 1937
Size: 1551 4594
Acc65I G'GTAC_C
Cuts at: 3466 3466
Size: 6145
AclI AA'CG_TT
Cuts at: 1563 4272 4645 1563
Size: 2709 373 3063
Fragments arranged by size:
3063 2709 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2418 2483 2663 4208 5592 5860 1632
Size: 786 65 180 1545 1384 268 1917
Fragments arranged by size:
1917 1545 1384 786 268 180 65
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5948
AflIII A'CryG_T
Cuts at: 1203 3249 5044 6021 1203
Size: 2046 1795 977 1327
Fragments arranged by size:
2046 1795 1327 977
AgeI A'CCGG_T
Cuts at: 4976 4976
Size: 6145
AhdI GACnn_n'nnGTC
Cuts at: 2365 4874 2365
Size: 2509 3636
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6145
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6145
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2250 2282 2720 2752 2250
Size: 32 438 32 5643
Fragments arranged by size:
5643 438 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2396 3888 5460 2396
Size: 1492 1572 3081
Fragments arranged by size:
3081 1572 1492
ApaI G_GGCC'C
Cuts at: 2755 2755
Size: 6145
ApaLI G'TGCA_C
Cuts at: 4202 5358 4202
Size: 1156 4989
ApoI r'AATT_y
Cuts at: 1493 3454 3680 3832 5941 1493
Size: 1961 226 152 2109 1697
Fragments arranged by size:
2109 1961 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4699 160
Size: 872 3667 1606
Fragments arranged by size:
3667 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
AvaI C'yCGr_G
Cuts at: 1870 1943 2724 2957 3125 3470 1870
Size: 73 781 233 168 345 4545
Fragments arranged by size:
4545 781 345 233 168 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2930 2963 2930
Size: 33 6112
BamHI G'GATC_C
Cuts at: 3475 3842 3475
Size: 367 5778
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2102 2622 2912 3466 4921
Size: 325 217 627 315 520 290 554 1455
Cuts at: 4921 618
Size: 1842
Fragments arranged by size:
1842 1455 627 554 520 325 315 290
217
BanII G_rGCy'C
Cuts at: 1391 2375 2755 3084 3191 3464 1391
Size: 984 380 329 107 273 4072
Fragments arranged by size:
4072 984 380 329 273 107
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2201 2930 928
Size: 971 302 729 4143
Fragments arranged by size:
4143 971 729 302
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4336 4370 1903
Size: 34 2399 34 3678
Fragments arranged by size:
3678 2399 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4037 5247 4037
Size: 1210 4935
BclI T'GATC_A
Cuts at: 1548 3342 1548
Size: 1794 4351
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4756 136
Size: 107 122 71 631 605 3084 1525
Fragments arranged by size:
3084 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6140 6140
Size: 6145
BlpI GC'TnA_GC
Cuts at: 3266 3562 3266
Size: 296 5849
Bme1580I G_kGCm'C
Cuts at: 1790 2483 2755 2865 4206 5362 1790
Size: 693 272 110 1341 1156 2573
Fragments arranged by size:
2573 1341 1156 693 272 110
BmgBI CAC'GTC
Cuts at: 1318 2014 3294 1318
Size: 696 1280 4169
Fragments arranged by size:
4169 1280 696
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2373 3409 3876
Size: 416 53 83 399 24 956 1036 467
Cuts at: 3876 4834 442
Size: 958 1753
Fragments arranged by size:
1753 1036 958 956 467 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6145
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2602 2855 2987 4805 1239
Size: 1363 253 132 1818 2579
Fragments arranged by size:
2579 1818 1363 253 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2075 2318 2531 2813 3140 4270 5135 5433 2075
Size: 243 213 282 327 1130 865 298 2787
Fragments arranged by size:
2787 1130 865 327 298 282 243 213
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4808 882
Size: 617 3309 2219
Fragments arranged by size:
3309 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3250 3936 493
Size: 711 2046 686 2702
Fragments arranged by size:
2702 2046 711 686
BsaBI GATnn'nnATC
Cuts at: 3347 6139 3347
Size: 2792 3353
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3198 3952 4334 4969
Size: 53 83 186 936 1665 754 382 635
Cuts at: 4969 275
Size: 1451
Fragments arranged by size:
1665 1451 936 754 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2364 4578 4976 5250 5397 5808 2364
Size: 2214 398 274 147 411 2701
Fragments arranged by size:
2701 2214 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2720 2750 1178
Size: 30 165 30 1317 30 4573
Fragments arranged by size:
4573 1317 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 2300 2309 2360 2363 2366 3110 3338
Size: 892 222 9 51 3 3 744 228
Cuts at: 3338 1186
Size: 3993
Fragments arranged by size:
3993 892 744 228 222 51 9 3
3
BseYI C'CCAG_C
Cuts at: 2470 3007 5348 5844 2470
Size: 537 2341 496 2771
Fragments arranged by size:
2771 2341 537 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2024 2780 3009 1163
Size: 573 288 756 229 4299
Fragments arranged by size:
4299 756 573 288 229
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2129 2369 2668 2986 3040 3444
Size: 275 186 128 240 299 318 54 404
Cuts at: 3444 3595 4356 5384 1540
Size: 151 761 1028 2301
Fragments arranged by size:
2301 1028 761 404 318 299 275 240
186 151 128 54
BsiHKAI G_wGCw'C
Cuts at: 3084 4206 4291 5362 3084
Size: 1122 85 1071 3867
Fragments arranged by size:
3867 1122 1071 85
BsmI GAATG_Cn'
Cuts at: 3656 3749 3656
Size: 93 6052
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2375 2483 2755 2865 3084 3191 3464
Size: 399 585 108 272 110 219 107 273
Cuts at: 3464 4206 4291 5362 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 585 399 273 272 219
110 108 107 85
BspEI T'CCGG_A
Cuts at: 5808 5808
Size: 6145
BspHI T'CATG_A
Cuts at: 4034 5764 4034
Size: 1730 4415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2802 3500 844
Size: 1958 698 3489
Fragments arranged by size:
3489 1958 698
BsrBI CCG'CTC
Cuts at: 1394 1823 2125 2203 2290 4032 1394
Size: 429 302 78 87 1742 3507
Fragments arranged by size:
3507 1742 429 302 87 78
BsrDI GCAATG_nn'
Cuts at: 65 1248 4640 4814 65
Size: 1183 3392 174 1396
Fragments arranged by size:
3392 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3230 4789 4976 1700
Size: 110 1420 1559 187 2869
Fragments arranged by size:
2869 1559 1420 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6145
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6145
BssSI C'ACGA_G
Cuts at: 4205 5217 4205
Size: 1012 5133
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6145
BstBI TT'CG_AA
Cuts at: 4949 4949
Size: 6145
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6145
BstYI r'GATC_y
Cuts at: 1972 2541 3099 3475 3842 4227 4244 5696 5782
Size: 569 558 376 367 385 17 1452 86
Cuts at: 5782 5794 6140 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 569 558 385 376 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2283 2283
Size: 6145
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5593
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3203 487
Size: 683 56 1977 3429
Fragments arranged by size:
3429 1977 683 56
BtsI GCAGTG_nn'
Cuts at: 2186 2246 2997 3661 4455 4475 2186
Size: 60 751 664 794 20 3856
Fragments arranged by size:
3856 794 751 664 60 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6145
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6110
DraI TTT'AAA
Cuts at: 3449 3449
Size: 6145
DraIII CAC_nnn'GTG
Cuts at: 2381 2437 2381
Size: 56 6089
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5152 809
Size: 507 3836 1802
Fragments arranged by size:
3836 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2072 2126 2418
Size: 54 1425 50 275 18 242 54 292
Cuts at: 2418 2565 2983 3429 3441 3592 4481 6110 8
Size: 147 418 446 12 151 889 1629 43
Fragments arranged by size:
1629 1425 889 446 418 292 275 242
151 147 54 54 50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2126 2983 3441 3592 1537
Size: 275 314 857 458 151 4090
Fragments arranged by size:
4090 857 458 314 275 151
EarI CTCTTCn'nnn_
Cuts at: 1353 2394 4075 1353
Size: 1041 1681 3423
Fragments arranged by size:
3423 1681 1041
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4732 5106 5252 387
Size: 1064 138 3143 374 146 1280
Fragments arranged by size:
3143 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3082 3082
Size: 6145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2418 2483 2602 2663 2855 2987 4208
Size: 393 786 65 119 61 192 132 1221
Cuts at: 4208 4805 5592 5860 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1221 787 786 597 393 268 192
132 119 65 61
EcoNI CCTnn'n_nnAGG
Cuts at: 2504 2549 3092 2504
Size: 45 543 5557
Fragments arranged by size:
5557 543 45
EcoO109I rG'GnC_Cy
Cuts at: 2824 3163 3402 2824
Size: 339 239 5567
Fragments arranged by size:
5567 339 239
EcoRI G'AATT_C
Cuts at: 3454 3832 3454
Size: 378 5767
FspI TGC'GCA
Cuts at: 4651 4651
Size: 6145
HaeII r_GCGC'y
Cuts at: 1678 2769 5292 1678
Size: 1091 2523 2531
Fragments arranged by size:
2531 2523 1091
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2351 2383 2762 2794 3022
Size: 32 135 32 779 32 379 32 228
Cuts at: 3022 3054 3119 3151 4786 4818 4860 4892 1373
Size: 32 65 32 1635 32 42 32 2626
Fragments arranged by size:
2626 1635 779 379 228 135 65 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3489 3735 669
Size: 988 281 1551 246 3079
Fragments arranged by size:
3079 1551 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3071 748
Size: 2323 3822
HpaI GTT'AAC
Cuts at: 3735 3735
Size: 6145
KpnI G_GTAC'C
Cuts at: 3470 3470
Size: 6145
MfeI C'AATT_G
Cuts at: 3744 3744
Size: 6145
MluI A'CGCG_T
Cuts at: 6021 6021
Size: 6145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3276 5259 5443 1305
Size: 1971 1983 184 2007
Fragments arranged by size:
2007 1983 1971 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2420 2567 10
Size: 54 1425 343 588 147 3588
Fragments arranged by size:
3588 1425 588 343 147 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3089 4103 4462 4621 518
Size: 648 1923 1014 359 159 2042
Fragments arranged by size:
2042 1923 1014 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2188 2232 3435 3561 4238 5386 5631 1606
Size: 582 44 1203 126 677 1148 245 2120
Fragments arranged by size:
2120 1203 1148 677 582 245 126 44
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5593
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6145
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6145
NotI GC'GGCC_GC
Cuts at: 2126 3592 2126
Size: 1466 4679
NspI r_CATG'y
Cuts at: 1132 1229 3503 5048 1132
Size: 97 2274 1545 2229
Fragments arranged by size:
2274 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6145
PciI A'CATG_T
Cuts at: 5044 5044
Size: 6145
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2320 1108
Size: 1212 4933
PmeI GTTT'AAAC
Cuts at: 3449 3449
Size: 6145
PmlI CAC'GTG
Cuts at: 3250 3250
Size: 6145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2720 2752 4214 4246 5727 5759 2720
Size: 32 1462 32 1481 32 3106
Fragments arranged by size:
3106 1481 1462 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2824 3163 2824
Size: 339 5806
PshAI GACnn'nnGTC
Cuts at: 1613 2249 1613
Size: 636 5509
PsiI TTA'TAA
Cuts at: 3715 3715
Size: 6145
PspOMI G'GGCC_C
Cuts at: 2751 2751
Size: 6145
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2801 2833 2801
Size: 32 6113
PstI C_TGCA'G
Cuts at: 830 2241 2613 3497 830
Size: 1411 372 884 3478
Fragments arranged by size:
3478 1411 884 372
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6145
PvuII CAG'CTG
Cuts at: 2188 2188
Size: 6145
SacI G_AGCT'C
Cuts at: 3084 3084
Size: 6145
SalI G'TCGA_C
Cuts at: 1936 3487 1936
Size: 1551 4594
SbfI CC_TGCA'GG
Cuts at: 3497 3497
Size: 6145
ScaI AGT'ACT
Cuts at: 2648 4393 6105 2648
Size: 1745 1712 2688
Fragments arranged by size:
2688 1745 1712
SexAI A'CCwGG_T
Cuts at: 3308 3308
Size: 6145
SfcI C'TryA_G
Cuts at: 826 1045 2237 2609 3493 4628 5309 5500 826
Size: 219 1192 372 884 1135 681 191 1471
Fragments arranged by size:
1471 1192 1135 884 681 372 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6145
SmaI CCC'GGG
Cuts at: 1872 2726 3472 1872
Size: 854 746 4545
Fragments arranged by size:
4545 854 746
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2090 2333 2546 2828 3155 4249
Size: 197 926 147 243 213 282 327 1094
Cuts at: 4249 5150 5412 820
Size: 901 262 1553
Fragments arranged by size:
1553 1094 926 901 327 282 262 243
213 197 147
SnaBI TAC'GTA
Cuts at: 493 3936 493
Size: 3443 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6145
SphI G_CATG'C
Cuts at: 1132 3503 1132
Size: 2371 3774
SspI AAT'ATT
Cuts at: 5 52 4069 5
Size: 47 4017 2081
Fragments arranged by size:
4017 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6145
StyI C'CwwG_G
Cuts at: 513 1065 2568 3201 3584 513
Size: 552 1503 633 383 3074
Fragments arranged by size:
3074 1503 633 552 383
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2392 4172 4189 4342 4527 4972 325
Size: 1255 812 1780 17 153 185 445 1498
Fragments arranged by size:
1780 1498 1255 812 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2055 2646 4391
Size: 274 80 33 51 1443 78 591 1745
Cuts at: 4391 6103 96
Size: 1712 138
Fragments arranged by size:
1745 1712 1443 591 274 138 80 78
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2067 2099 3106 3138 6105 6137 2067
Size: 32 1007 32 2967 32 2075
Fragments arranged by size:
2967 2075 1007 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6145
XbaI T'CTAG_A
Cuts at: 3481 3481
Size: 6145
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2317 2575 2011
Size: 306 258 5581
Fragments arranged by size:
5581 306 258
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6145
XmaI C'CCGG_G
Cuts at: 1870 2724 3470 1870
Size: 854 746 4545
Fragments arranged by size:
4545 854 746
XmnI GAAnn'nnTTC
Cuts at: 4274 4274
Size: 6145
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3953 276
Size: 53 83 186 936 2419 2468
Fragments arranged by size:
2468 2419 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PspXI RsrII SacII SanDI SapI SfiI SfoI
SrfI SwaI