Restriction map for
pFN21AB5678
(Circular) (Six-base) MAPSORT of: pFN21AB5678.seq Check: 1881 from: 1 to: 7039
pFN21AB5678 7039 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4849 278
Size: 53 83 186 936 3313 2468
Fragments arranged by size:
3313 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4382 1937
Size: 2445 4594
Acc65I G'GTAC_C
Cuts at: 4360 4360
Size: 7039
AclI AA'CG_TT
Cuts at: 1563 5166 5539 1563
Size: 3603 373 3063
Fragments arranged by size:
3603 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3401 4089 5102 6486 6754 1632
Size: 1769 688 1013 1384 268 1917
Fragments arranged by size:
1917 1769 1384 1013 688 268
AfeI AGC'GCT
Cuts at: 3485 3485
Size: 7039
AflII C'TTAA_G
Cuts at: 820 1017 3455 820
Size: 197 2438 4404
Fragments arranged by size:
4404 2438 197
AflIII A'CryG_T
Cuts at: 1203 5938 6915 1203
Size: 4735 977 1327
Fragments arranged by size:
4735 1327 977
AgeI A'CCGG_T
Cuts at: 5870 5870
Size: 7039
AhdI GACnn_n'nnGTC
Cuts at: 5768 5768
Size: 7039
AleI CACnn'nnGTG
Cuts at: 1166 3470 1166
Size: 2304 4735
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7039
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3881 3913 3881
Size: 32 7007
AlwNI CAG_nnn'CTG
Cuts at: 2213 2716 3328 3389 4782 6354 2213
Size: 503 612 61 1393 1572 2898
Fragments arranged by size:
2898 1572 1393 612 503 61
ApaI G_GGCC'C
Cuts at: 2474 2809 4116 2474
Size: 335 1307 5397
Fragments arranged by size:
5397 1307 335
ApaLI G'TGCA_C
Cuts at: 5096 6252 5096
Size: 1156 5883
ApoI r'AATT_y
Cuts at: 1493 3572 4191 4348 4574 4726 6835 1493
Size: 2079 619 157 226 152 2109 1697
Fragments arranged by size:
2109 2079 1697 619 226 157 152
AseI AT'TA_AT
Cuts at: 160 1032 2760 5593 160
Size: 872 1728 2833 1606
Fragments arranged by size:
2833 1728 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7039
AvaI C'yCGr_G
Cuts at: 1870 1943 3399 3635 4062 4364 1870
Size: 73 1456 236 427 302 4545
Fragments arranged by size:
4545 1456 427 302 236 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2638 2671 2638
Size: 33 7006
BamHI G'GATC_C
Cuts at: 3288 4369 4736 3288
Size: 1081 367 5591
Fragments arranged by size:
5591 1081 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3472 4313 4360 5815 618
Size: 325 217 627 1685 841 47 1455 1842
Fragments arranged by size:
1842 1685 1455 841 627 325 217 47
BanII G_rGCy'C
Cuts at: 1391 2474 2809 2972 4116 4358 1391
Size: 1083 335 163 1144 242 4072
Fragments arranged by size:
4072 1144 1083 335 242 163
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2073 928
Size: 971 174 5894
Fragments arranged by size:
5894 971 174
BbvCI CC'TCA_GC
Cuts at: 2052 4050 2052
Size: 1998 5041
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5230 5264 1903
Size: 34 3293 34 3678
Fragments arranged by size:
3678 3293 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4931 6141 4931
Size: 1210 5829
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7039
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2040 2444 2618
Size: 107 122 71 631 605 368 404 174
Cuts at: 2618 2813 3740 5650 136
Size: 195 927 1910 1525
Fragments arranged by size:
1910 1525 927 631 605 404 368 195
174 122 107 71
BglII A'GATC_T
Cuts at: 2109 3808 7034 2109
Size: 1699 3226 2114
Fragments arranged by size:
3226 2114 1699
BlpI GC'TnA_GC
Cuts at: 4456 4456
Size: 7039
Bme1580I G_kGCm'C
Cuts at: 1790 2316 2474 2809 3477 3873 4116 5100 6256
Size: 526 158 335 668 396 243 984 1156
Cuts at: 6256 1790
Size: 2573
Fragments arranged by size:
2573 1156 984 668 526 396 335 243
158
BmgBI CAC'GTC
Cuts at: 1318 2963 1318
Size: 1645 5394
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3002 3020 3038
Size: 416 53 83 399 24 1585 18 18
Cuts at: 3038 3056 3665 4770 5728 442
Size: 18 609 1105 958 1753
Fragments arranged by size:
1753 1585 1105 958 609 416 399 83
53 24 18 18 18
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7039
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2738 2848 2958 3174 4138 4219 5699 1239
Size: 1499 110 110 216 964 81 1480 2579
Fragments arranged by size:
2579 1499 1480 964 216 110 110 81
Bpu10I CC'TnA_GC
Cuts at: 2052 4050 2052
Size: 1998 5041
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5164 6029 6327 5164
Size: 865 298 5876
Fragments arranged by size:
5876 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2636 3692 3969 5702 882
Size: 617 1137 1056 277 1733 2219
Fragments arranged by size:
2219 1733 1137 1056 617 277
BsaAI yAC'GTr
Cuts at: 493 1204 4830 493
Size: 711 3626 2702
Fragments arranged by size:
3626 2702 711
BsaBI GATnn'nnATC
Cuts at: 3528 7033 3528
Size: 3505 3534
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2897 4846 5228 5863
Size: 53 83 186 936 1364 1949 382 635
Cuts at: 5863 275
Size: 1451
Fragments arranged by size:
1949 1451 1364 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5472 5870 6144 6291 6702 5472
Size: 398 274 147 411 5809
Fragments arranged by size:
5809 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3783 3813 3881 3911 1178
Size: 30 165 30 2380 30 68 30 4306
Fragments arranged by size:
4306 2380 165 68 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 3680 1186
Size: 841 1653 4545
Fragments arranged by size:
4545 1653 841
BseYI C'CCAG_C
Cuts at: 4122 6242 6738 4122
Size: 2120 496 4423
Fragments arranged by size:
4423 2120 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2664 2820 3375 3978 1163
Size: 573 928 156 555 603 4224
Fragments arranged by size:
4224 928 603 573 555 156
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4489 5250 6278 1540
Size: 275 186 2488 761 1028 2301
Fragments arranged by size:
2488 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2889 5100 5185 6256 2889
Size: 2211 85 1071 3672
Fragments arranged by size:
3672 2211 1071 85
BsmI GAATG_Cn'
Cuts at: 2254 2458 4231 4550 4643 2254
Size: 204 1773 319 93 4650
Fragments arranged by size:
4650 1773 319 204 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2316 2474 2809 2889 2972 3477 3873
Size: 399 526 158 335 80 83 505 396
Cuts at: 3873 4116 4358 5100 5185 6256 1391
Size: 243 242 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 526 505 399 396 335
243 242 158 85 83 80
BspEI T'CCGG_A
Cuts at: 6702 6702
Size: 7039
BspHI T'CATG_A
Cuts at: 3717 4928 6658 3717
Size: 1211 1730 4098
Fragments arranged by size:
4098 1730 1211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3336 3354 4394 844
Size: 2492 18 1040 3489
Fragments arranged by size:
3489 2492 1040 18
BsrBI CCG'CTC
Cuts at: 1394 1823 4233 4926 1394
Size: 429 2410 693 3507
Fragments arranged by size:
3507 2410 693 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3954 5534 5708 65
Size: 1183 2706 1580 174 1396
Fragments arranged by size:
2706 1580 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4256 5683 5870 1700
Size: 110 2446 1427 187 2869
Fragments arranged by size:
2869 2446 1427 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7039
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7039
BssSI C'ACGA_G
Cuts at: 3981 5099 6111 3981
Size: 1118 1012 4909
Fragments arranged by size:
4909 1118 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3328 794
Size: 2534 4505
BstBI TT'CG_AA
Cuts at: 5843 5843
Size: 7039
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7039
BstXI CCAn_nnnn'nTGG
Cuts at: 2188 2518 2907 2997 3015 3033 3051 3069 3123
Size: 330 389 90 18 18 18 18 54
Cuts at: 3123 3141 3159 3177 3231 3249 3285 3318 2188
Size: 18 18 18 54 18 36 33 5909
Fragments arranged by size:
5909 389 330 90 54 54 36 33
18 18 18 18 18 18 18 18
BstYI r'GATC_y
Cuts at: 1972 2109 3288 3808 4177 4369 4736 5121 5138
Size: 137 1179 520 369 192 367 385 17
Cuts at: 5138 6590 6676 6688 7034 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1179 520 385 369 367 346
192 137 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2920 2920
Size: 7039
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5548
Fragments arranged by size:
5548 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6300
Fragments arranged by size:
6300 683 56
BtsI GCAGTG_nn'
Cuts at: 2794 3236 4555 5349 5369 2794
Size: 442 1319 794 20 4464
Fragments arranged by size:
4464 1319 794 442 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7039
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7004
DraI TTT'AAA
Cuts at: 2239 4343 2239
Size: 2104 4935
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2830 6046 809
Size: 507 1514 3216 1802
Fragments arranged by size:
3216 1802 1514 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2520 2619 3035
Size: 54 1425 50 275 18 690 99 416
Cuts at: 3035 3071 3107 3215 3233 3251 3269 3302 4486
Size: 36 36 108 18 18 18 33 1184
Cuts at: 4486 5375 7004 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1184 889 690 416 275 108
99 54 50 43 36 36 33 18
18 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 4486 1537
Size: 275 2674 4090
Fragments arranged by size:
4090 2674 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2772 4969 1353
Size: 1419 2197 3423
Fragments arranged by size:
3423 2197 1419
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5626 6000 6146 387
Size: 1064 138 4037 374 146 1280
Fragments arranged by size:
4037 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2738 2848 2958 3174 3401 4089 4138
Size: 393 1106 110 110 216 227 688 49
Cuts at: 4138 4219 5102 5699 6486 6754 1239
Size: 81 883 597 787 268 1524
Fragments arranged by size:
1524 1106 883 787 688 597 393 268
227 216 110 110 81 49
EcoNI CCTnn'n_nnAGG
Cuts at: 3416 3752 3416
Size: 336 6703
EcoO109I rG'GnC_Cy
Cuts at: 2572 2748 2806 2823 3185 3203 3907 4113 2572
Size: 176 58 17 362 18 704 206 5498
Fragments arranged by size:
5498 704 362 206 176 58 18 17
EcoRI G'AATT_C
Cuts at: 4191 4348 4726 4191
Size: 157 378 6504
Fragments arranged by size:
6504 378 157
EcoRV GAT'ATC
Cuts at: 3505 3505
Size: 7039
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2251 2353 2251
Size: 102 6937
FspI TGC'GCA
Cuts at: 5545 5545
Size: 7039
HaeII r_GCGC'y
Cuts at: 1678 2076 3487 6186 1678
Size: 398 1411 2699 2531
Fragments arranged by size:
2699 2531 1411 398
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3492 3524 3783 3815 4091
Size: 32 135 32 1920 32 259 32 276
Cuts at: 4091 4123 4169 4201 5680 5712 5754 5786 1373
Size: 32 46 32 1479 32 42 32 2626
Fragments arranged by size:
2626 1920 1479 276 259 135 46 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4383 4629 669
Size: 988 281 2445 246 3079
Fragments arranged by size:
3079 2445 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7039
HpaI GTT'AAC
Cuts at: 4629 4629
Size: 7039
KpnI G_GTAC'C
Cuts at: 4364 4364
Size: 7039
MfeI C'AATT_G
Cuts at: 4638 4638
Size: 7039
MluI A'CGCG_T
Cuts at: 6915 6915
Size: 7039
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6153 6337 1305
Size: 4848 184 2007
Fragments arranged by size:
4848 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2522 2621 3037 3073 3235
Size: 54 1425 343 690 99 416 36 162
Cuts at: 3235 3253 3271 3304 10
Size: 18 18 33 3745
Fragments arranged by size:
3745 1425 690 416 343 162 99 54
36 33 18 18
MslI CAynn'nnrTG
Cuts at: 518 1166 3139 3157 3470 3716 4224 4997 5356
Size: 648 1973 18 313 246 508 773 359
Cuts at: 5356 5515 518
Size: 159 2042
Fragments arranged by size:
2042 1973 773 648 508 359 313 246
159 18
MspA1I CmG'CkG
Cuts at: 1606 2147 2213 2582 3296 4049 4455 5132 6280
Size: 541 66 369 714 753 406 677 1148
Cuts at: 6280 6525 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 753 714 677 541 406 369
245 66
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5548
Fragments arranged by size:
5548 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7039
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7039
NotI GC'GGCC_GC
Cuts at: 4486 4486
Size: 7039
NspI r_CATG'y
Cuts at: 1132 1229 3555 4397 5942 1132
Size: 97 2326 842 1545 2229
Fragments arranged by size:
2326 2229 1545 842 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7039
PciI A'CATG_T
Cuts at: 5938 5938
Size: 7039
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3087 3267 1108
Size: 1979 180 4880
Fragments arranged by size:
4880 1979 180
PfoI T'CCnGG_A
Cuts at: 2723 2723
Size: 7039
PmeI GTTT'AAAC
Cuts at: 4343 4343
Size: 7039
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3881 3913 5108 5140 6621 6653 3881
Size: 32 1195 32 1481 32 4267
Fragments arranged by size:
4267 1481 1195 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2748 2823 3185 3203 2748
Size: 75 362 18 6584
Fragments arranged by size:
6584 362 75 18
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7039
PsiI TTA'TAA
Cuts at: 4609 4609
Size: 7039
PspOMI G'GGCC_C
Cuts at: 2470 2805 4112 2470
Size: 335 1307 5397
Fragments arranged by size:
5397 1307 335
PstI C_TGCA'G
Cuts at: 830 2821 4015 4048 4391 830
Size: 1991 1194 33 343 3478
Fragments arranged by size:
3478 1991 1194 343 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7039
PvuII CAG'CTG
Cuts at: 2147 2213 2582 4049 2147
Size: 66 369 1467 5137
Fragments arranged by size:
5137 1467 369 66
SalI G'TCGA_C
Cuts at: 1936 4381 1936
Size: 2445 4594
SbfI CC_TGCA'GG
Cuts at: 4391 4391
Size: 7039
ScaI AGT'ACT
Cuts at: 3446 5287 6999 3446
Size: 1841 1712 3486
Fragments arranged by size:
3486 1841 1712
SfcI C'TryA_G
Cuts at: 826 1045 2817 3859 4011 4044 4387 5522 6203
Size: 219 1772 1042 152 33 343 1135 681
Cuts at: 6203 6394 826
Size: 191 1471
Fragments arranged by size:
1772 1471 1135 1042 681 343 219 191
152 33
SfiI GGCCn_nnn'nGGCC
Cuts at: 2444 2618 2444
Size: 174 6865
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7039
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7039
SmaI CCC'GGG
Cuts at: 1872 4064 4366 1872
Size: 2192 302 4545
Fragments arranged by size:
4545 2192 302
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3455 5143 6044 6306 820
Size: 197 926 1512 1688 901 262 1553
Fragments arranged by size:
1688 1553 1512 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4830 493
Size: 4337 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7039
SphI G_CATG'C
Cuts at: 1132 3555 4397 1132
Size: 2423 842 3774
Fragments arranged by size:
3774 2423 842
SrfI GCCC'GGGC
Cuts at: 4064 4064
Size: 7039
SspI AAT'ATT
Cuts at: 5 52 4963 5
Size: 47 4911 2081
Fragments arranged by size:
4911 2081 47
StuI AGG'CCT
Cuts at: 1517 2057 2224 2533 4022 1517
Size: 540 167 309 1489 4534
Fragments arranged by size:
4534 1489 540 309 167
StyI C'CwwG_G
Cuts at: 513 1065 2004 2304 2328 3802 4023 4478 513
Size: 552 939 300 24 1474 221 455 3074
Fragments arranged by size:
3074 1474 939 552 455 300 221 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2660 3674 3691 3695 5066 5083 5236
Size: 1255 1080 1014 17 4 1371 17 153
Cuts at: 5236 5421 5866 325
Size: 185 445 1498
Fragments arranged by size:
1498 1371 1255 1080 1014 445 185 153
17 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3444 5285 6997
Size: 274 80 33 51 1443 1467 1841 1712
Cuts at: 6997 96
Size: 138
Fragments arranged by size:
1841 1712 1467 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6999 7031 6999
Size: 32 7007
Tth111I GACn'n_nGTC
Cuts at: 1316 3777 1316
Size: 2461 4578
XbaI T'CTAG_A
Cuts at: 4375 4375
Size: 7039
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2464 4203 2464
Size: 1739 5300
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7039
XmaI C'CCGG_G
Cuts at: 1870 4062 4364 1870
Size: 2192 302 4545
Fragments arranged by size:
4545 2192 302
XmnI GAAnn'nnTTC
Cuts at: 5168 5168
Size: 7039
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4847 276
Size: 53 83 186 936 3313 2468
Fragments arranged by size:
3313 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI
BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfoI SwaI