Restriction map for
pFN21AB5676
(Circular) (Six-base) MAPSORT of: pFN21AB5676.seq Check: 5277 from: 1 to: 7123
pFN21AB5676 7123 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4933 278
Size: 53 83 186 936 3397 2468
Fragments arranged by size:
3397 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2403 4389 4466 1937
Size: 466 1986 77 4594
Fragments arranged by size:
4594 1986 466 77
Acc65I G'GTAC_C
Cuts at: 4444 4444
Size: 7123
AclI AA'CG_TT
Cuts at: 1563 5250 5623 1563
Size: 3687 373 3063
Fragments arranged by size:
3687 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3131 3536 4274 4300 4311 5186 6570 6838
Size: 1499 405 738 26 11 875 1384 268
Cuts at: 6838 1632
Size: 1917
Fragments arranged by size:
1917 1499 1384 875 738 405 268 26
11
AflII C'TTAA_G
Cuts at: 820 1017 2408 820
Size: 197 1391 5535
Fragments arranged by size:
5535 1391 197
AflIII A'CryG_T
Cuts at: 1203 3022 6022 6999 1203
Size: 1819 3000 977 1327
Fragments arranged by size:
3000 1819 1327 977
AgeI A'CCGG_T
Cuts at: 5954 5954
Size: 7123
AhdI GACnn_n'nnGTC
Cuts at: 5852 5852
Size: 7123
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7123
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3243 3262 1184
Size: 2059 19 5045
Fragments arranged by size:
5045 2059 19
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4007 4039 4007
Size: 32 7091
AlwNI CAG_nnn'CTG
Cuts at: 3257 3478 3817 4190 4866 6438 3257
Size: 221 339 373 676 1572 3942
Fragments arranged by size:
3942 1572 676 373 339 221
ApaLI G'TGCA_C
Cuts at: 5180 6336 5180
Size: 1156 5967
ApoI r'AATT_y
Cuts at: 1493 3114 3509 4432 4658 4810 6919 1493
Size: 1621 395 923 226 152 2109 1697
Fragments arranged by size:
2109 1697 1621 923 395 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5677 160
Size: 872 4645 1606
Fragments arranged by size:
4645 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7123
AvaI C'yCGr_G
Cuts at: 1870 1943 2769 4448 1870
Size: 73 826 1679 4545
Fragments arranged by size:
4545 1679 826 73
AvrII C'CTAG_G
Cuts at: 2290 2290
Size: 7123
BamHI G'GATC_C
Cuts at: 4453 4820 4453
Size: 367 6756
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3415 4444 5899 618
Size: 325 217 627 1628 1029 1455 1842
Fragments arranged by size:
1842 1628 1455 1029 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4442 1391
Size: 3051 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3441 4404 928
Size: 971 1542 963 3647
Fragments arranged by size:
3647 1542 971 963
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5314 5348 1903
Size: 34 3377 34 3678
Fragments arranged by size:
3678 3377 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2354 3498 5015 6225 2354
Size: 1144 1517 1210 3252
Fragments arranged by size:
3252 1517 1210 1144
BclI T'GATC_A
Cuts at: 1548 2808 3922 1548
Size: 1260 1114 4749
Fragments arranged by size:
4749 1260 1114
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5734 136
Size: 107 122 71 631 605 4062 1525
Fragments arranged by size:
4062 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7118 7118
Size: 7123
BlpI GC'TnA_GC
Cuts at: 4540 4540
Size: 7123
Bme1580I G_kGCm'C
Cuts at: 1790 3022 5184 6340 1790
Size: 1232 2162 1156 2573
Fragments arranged by size:
2573 2162 1232 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7123
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3992 4347 4854
Size: 416 53 83 399 24 2575 355 507
Cuts at: 4854 5812 442
Size: 958 1753
Fragments arranged by size:
2575 1753 958 507 416 399 355 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2645 1056
Size: 1589 5534
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2034 2520 2811 4074 5783 1239
Size: 795 486 291 1263 1709 2579
Fragments arranged by size:
2579 1709 1263 795 486 291
Bpu10I CC'TnA_GC
Cuts at: 3639 3639
Size: 7123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3391 3959 5248 6113 6411 3391
Size: 568 1289 865 298 4103
Fragments arranged by size:
4103 1289 865 568 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3302 5786 882
Size: 617 1803 2484 2219
Fragments arranged by size:
2484 2219 1803 617
BsaAI yAC'GTr
Cuts at: 493 1204 4914 493
Size: 711 3710 2702
Fragments arranged by size:
3710 2702 711
BsaBI GATnn'nnATC
Cuts at: 7117 7117
Size: 7123
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4930 5312 5947 275
Size: 53 83 186 936 3397 382 635 1451
Fragments arranged by size:
3397 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2442 3360 5556 5954 6228 6375 6786 2442
Size: 918 2196 398 274 147 411 2779
Fragments arranged by size:
2779 2196 918 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3608 3638 1178
Size: 30 165 30 2205 30 4663
Fragments arranged by size:
4663 2205 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2891 1186
Size: 1705 5418
BseYI C'CCAG_C
Cuts at: 2130 3067 6326 6822 2130
Size: 937 3259 496 2431
Fragments arranged by size:
3259 2431 937 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6550
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4573 5334 6362 1540
Size: 275 186 2572 761 1028 2301
Fragments arranged by size:
2572 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3251 3501 5184 5269 6340 3251
Size: 250 1683 85 1071 4034
Fragments arranged by size:
4034 1683 1071 250 85
BsmI GAATG_Cn'
Cuts at: 2896 3611 4634 4727 2896
Size: 715 1023 93 5292
Fragments arranged by size:
5292 1023 715 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2188 2188
Size: 7123
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3022 3251 3501 4442 5184 5269 6340
Size: 399 1232 229 250 941 742 85 1071
Cuts at: 6340 1391
Size: 2174
Fragments arranged by size:
2174 1232 1071 941 742 399 250 229
85
BspEI T'CCGG_A
Cuts at: 2442 6786 2442
Size: 4344 2779
BspHI T'CATG_A
Cuts at: 2826 3327 5012 6742 2826
Size: 501 1685 1730 3207
Fragments arranged by size:
3207 1730 1685 501
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3993 4478 844
Size: 3149 485 3489
Fragments arranged by size:
3489 3149 485
BsrBI CCG'CTC
Cuts at: 1394 1823 2968 5010 1394
Size: 429 1145 2042 3507
Fragments arranged by size:
3507 2042 1145 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3594 5618 5792 65
Size: 1183 2346 2024 174 1396
Fragments arranged by size:
2346 2024 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3876 5767 5954 1700
Size: 110 2066 1891 187 2869
Fragments arranged by size:
2869 2066 1891 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7123
BssHII G'CGCG_C
Cuts at: 1925 4232 1925
Size: 2307 4816
BssSI C'ACGA_G
Cuts at: 3759 5183 6195 3759
Size: 1424 1012 4687
Fragments arranged by size:
4687 1424 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7123
BstBI TT'CG_AA
Cuts at: 5927 5927
Size: 7123
BstEII G'GTnAC_C
Cuts at: 1184 3424 1184
Size: 2240 4883
BstXI CCAn_nnnn'nTGG
Cuts at: 4012 4012
Size: 7123
BstYI r'GATC_y
Cuts at: 1972 2509 3150 3286 3813 4194 4453 4820 5205
Size: 537 641 136 527 381 259 367 385
Cuts at: 5205 5222 6674 6760 6772 7118 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 641 537 527 385 381 367
346 259 136 86 17 12
BstZ17I GTA'TAC
Cuts at: 2404 4390 2404
Size: 1986 5137
BtgI C'CryG_G
Cuts at: 513 1065 2004 2467 3405 4161 513
Size: 552 939 463 938 756 3475
Fragments arranged by size:
3475 939 938 756 552 463
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2914 487
Size: 683 56 1688 4696
Fragments arranged by size:
4696 1688 683 56
BtsI GCAGTG_nn'
Cuts at: 2551 3392 4369 4390 4639 5433 5453 2551
Size: 841 977 21 249 794 20 4221
Fragments arranged by size:
4221 977 841 794 249 21 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7123
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3742 3777 529
Size: 35 3178 35 3875
Fragments arranged by size:
3875 3178 35 35
DraI TTT'AAA
Cuts at: 4427 4427
Size: 7123
DraIII CAC_nnn'GTG
Cuts at: 3654 3654
Size: 7123
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3915 6130 809
Size: 507 2599 2215 1802
Fragments arranged by size:
2599 2215 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3585 3913 4095
Size: 54 1425 50 275 18 1755 328 182
Cuts at: 4095 4570 5459 7088 8
Size: 475 889 1629 43
Fragments arranged by size:
1755 1629 1425 889 475 328 275 182
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4570 1537
Size: 275 2758 4090
Fragments arranged by size:
4090 2758 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2186 2399 2583 4167 5053 1353
Size: 833 213 184 1584 886 3423
Fragments arranged by size:
3423 1584 886 833 213 184
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5710 6084 6230 387
Size: 1064 138 4121 374 146 1280
Fragments arranged by size:
4121 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 2520 2811 3131 3536 4074 4274
Size: 393 402 486 291 320 405 538 200
Cuts at: 4274 4300 4311 5186 5783 6570 6838 1239
Size: 26 11 875 597 787 268 1524
Fragments arranged by size:
1524 875 787 597 538 486 405 402
393 320 291 268 200 26 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2693 2718 2748 3194 2693
Size: 25 30 446 6622
Fragments arranged by size:
6622 446 30 25
EcoO109I rG'GnC_Cy
Cuts at: 3445 3635 3445
Size: 190 6933
EcoRI G'AATT_C
Cuts at: 3114 4432 4810 3114
Size: 1318 378 5427
Fragments arranged by size:
5427 1318 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2434 2434
Size: 7123
FspI TGC'GCA
Cuts at: 3989 5629 3989
Size: 1640 5483
HaeII r_GCGC'y
Cuts at: 1678 3870 6270 1678
Size: 2192 2400 2531
Fragments arranged by size:
2531 2400 2192
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2024 2056 2576 2608 2800
Size: 32 135 32 452 32 520 32 192
Cuts at: 2800 2832 3821 3853 3941 3973 5764 5796 5838
Size: 32 989 32 88 32 1791 32 42
Cuts at: 5838 5870 1373
Size: 32 2626
Fragments arranged by size:
2626 1791 989 520 452 192 135 88
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2991 4467 4713 669
Size: 988 281 1053 1476 246 3079
Fragments arranged by size:
3079 1476 1053 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7123
HpaI GTT'AAC
Cuts at: 4713 4713
Size: 7123
KpnI G_GTAC'C
Cuts at: 4448 4448
Size: 7123
MfeI C'AATT_G
Cuts at: 2700 4722 2700
Size: 2022 5101
MluI A'CGCG_T
Cuts at: 6999 6999
Size: 7123
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6237 6421 1305
Size: 4932 184 2007
Fragments arranged by size:
4932 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3587 4097 10
Size: 54 1425 343 1755 510 3036
Fragments arranged by size:
3036 1755 1425 510 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3219 3225 5081 5440 5599 518
Size: 648 2053 6 1856 359 159 2042
Fragments arranged by size:
2053 2042 1856 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 4539 5216 6364 6609 1606
Size: 2933 677 1148 245 2120
Fragments arranged by size:
2933 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 4161 513
Size: 552 939 2157 3475
Fragments arranged by size:
3475 2157 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7123
NheI G'CTAG_C
Cuts at: 1052 2641 1052
Size: 1589 5534
NotI GC'GGCC_GC
Cuts at: 4570 4570
Size: 7123
NspI r_CATG'y
Cuts at: 1132 1229 3026 3224 3838 4481 6026 1132
Size: 97 1797 198 614 643 1545 2229
Fragments arranged by size:
2229 1797 1545 643 614 198 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7123
PciI A'CATG_T
Cuts at: 3022 6022 3022
Size: 3000 4123
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7123
PfoI T'CCnGG_A
Cuts at: 2421 2743 2421
Size: 322 6801
PmeI GTTT'AAAC
Cuts at: 4427 4427
Size: 7123
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5192 5224 6705 6737 5192
Size: 32 1481 32 5578
Fragments arranged by size:
5578 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3635 3635
Size: 7123
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7123
PsiI TTA'TAA
Cuts at: 4693 4693
Size: 7123
PspXI vC'TCGA_Gb
Cuts at: 2769 2769
Size: 7123
PstI C_TGCA'G
Cuts at: 830 2633 4475 830
Size: 1803 1842 3478
Fragments arranged by size:
3478 1842 1803
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7123
SalI G'TCGA_C
Cuts at: 1936 4465 1936
Size: 2529 4594
SbfI CC_TGCA'GG
Cuts at: 4475 4475
Size: 7123
ScaI AGT'ACT
Cuts at: 5371 7083 5371
Size: 1712 5411
SfcI C'TryA_G
Cuts at: 826 1045 2629 2775 3126 3470 4471 5606 6287
Size: 219 1584 146 351 344 1001 1135 681
Cuts at: 6287 6478 826
Size: 191 1471
Fragments arranged by size:
1584 1471 1135 1001 681 351 344 219
191 146
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7123
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7123
SmaI CCC'GGG
Cuts at: 1872 4450 1872
Size: 2578 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2408 2769 3370 3974 5227 6128
Size: 197 926 465 361 601 604 1253 901
Cuts at: 6128 6390 820
Size: 262 1553
Fragments arranged by size:
1553 1253 926 901 604 601 465 361
262 197
SnaBI TAC'GTA
Cuts at: 493 4914 493
Size: 4421 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7123
SphI G_CATG'C
Cuts at: 1132 4481 1132
Size: 3349 3774
SspI AAT'ATT
Cuts at: 5 52 5047 5
Size: 47 4995 2081
Fragments arranged by size:
4995 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7123
StyI C'CwwG_G
Cuts at: 513 1065 2004 2290 3537 3753 4005 4161 4562
Size: 552 939 286 1247 216 252 156 401
Cuts at: 4562 513
Size: 3074
Fragments arranged by size:
3074 1247 939 552 401 286 252 216
156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3215 3516 3813 5150 5167 5320 5505
Size: 1255 1635 301 297 1337 17 153 185
Cuts at: 5505 5950 325
Size: 445 1498
Fragments arranged by size:
1635 1498 1337 1255 445 301 297 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5369 7081 96
Size: 274 80 33 51 1443 3392 1712 138
Fragments arranged by size:
3392 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4324 4356 7083 7115 4324
Size: 32 2727 32 4332
Fragments arranged by size:
4332 2727 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3800 1316
Size: 2484 4639
XbaI T'CTAG_A
Cuts at: 4459 4459
Size: 7123
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3426 4343 3426
Size: 917 6206
XhoI C'TCGA_G
Cuts at: 1943 2769 1943
Size: 826 6297
XmaI C'CCGG_G
Cuts at: 1870 4448 1870
Size: 2578 4545
XmnI GAAnn'nnTTC
Cuts at: 5252 5252
Size: 7123
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4931 276
Size: 53 83 186 936 3397 2468
Fragments arranged by size:
3397 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI BsiWI Bsu36I EcoICRI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI