Restriction map for
pFN21AB5674
(Circular) (Six-base) MAPSORT of: pFN21AB5674.seq Check: 73 from: 1 to: 6010
pFN21AB5674 6010 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2151 3820 278
Size: 53 83 186 936 615 1669 2468
Fragments arranged by size:
2468 1669 936 615 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3353 1937
Size: 1416 4594
Acc65I G'GTAC_C
Cuts at: 3331 3331
Size: 6010
AclI AA'CG_TT
Cuts at: 1563 4137 4510 1563
Size: 2574 373 3063
Fragments arranged by size:
3063 2574 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2463 3129 4073 5457 5725 1632
Size: 831 666 944 1384 268 1917
Fragments arranged by size:
1917 1384 944 831 666 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5813
AflIII A'CryG_T
Cuts at: 1203 3240 4909 5886 1203
Size: 2037 1669 977 1327
Fragments arranged by size:
2037 1669 1327 977
AgeI A'CCGG_T
Cuts at: 4841 4841
Size: 6010
AhdI GACnn_n'nnGTC
Cuts at: 4739 4739
Size: 6010
AleI CACnn'nnGTG
Cuts at: 1166 2649 1166
Size: 1483 4527
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2667 1184
Size: 1483 4527
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2006 2038 2006
Size: 32 5978
AlwNI CAG_nnn'CTG
Cuts at: 3753 5325 3753
Size: 1572 4438
ApaI G_GGCC'C
Cuts at: 2264 2634 2264
Size: 370 5640
ApaLI G'TGCA_C
Cuts at: 2642 4067 5223 2642
Size: 1425 1156 3429
Fragments arranged by size:
3429 1425 1156
ApoI r'AATT_y
Cuts at: 1493 2419 2840 3319 3545 3697 5806 1493
Size: 926 421 479 226 152 2109 1697
Fragments arranged by size:
2109 1697 926 479 421 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4564 160
Size: 872 3532 1606
Fragments arranged by size:
3532 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
AvaI C'yCGr_G
Cuts at: 1870 1943 2508 2912 3188 3335 1870
Size: 73 565 404 276 147 4545
Fragments arranged by size:
4545 565 404 276 147 73
BamHI G'GATC_C
Cuts at: 3340 3707 3340
Size: 367 5643
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2834 3146 3331 4786 618
Size: 325 217 627 1047 312 185 1455 1842
Fragments arranged by size:
1842 1455 1047 627 325 312 217 185
BanII G_rGCy'C
Cuts at: 1391 2264 2634 2907 3329 1391
Size: 873 370 273 422 4072
Fragments arranged by size:
4072 873 422 370 273
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2418 3131 928
Size: 971 519 713 3807
Fragments arranged by size:
3807 971 713 519
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2305 2339 3188 3222 4201 4235 1903
Size: 34 368 34 849 34 979 34 3678
Fragments arranged by size:
3678 979 849 368 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3902 5112 3902
Size: 1210 4800
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6010
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4621 136
Size: 107 122 71 631 605 2949 1525
Fragments arranged by size:
2949 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2514 6005 2514
Size: 3491 2519
BlpI GC'TnA_GC
Cuts at: 3427 3427
Size: 6010
Bme1580I G_kGCm'C
Cuts at: 1790 2264 2634 2646 3151 4071 5227 1790
Size: 474 370 12 505 920 1156 2573
Fragments arranged by size:
2573 1156 920 505 474 370 12
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6010
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3741 4699 442
Size: 416 53 83 399 24 2324 958 1753
Fragments arranged by size:
2324 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2921 2975 4670 1239
Size: 1682 54 1695 2579
Fragments arranged by size:
2579 1695 1682 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4135 5000 5298 4135
Size: 865 298 4847
Fragments arranged by size:
4847 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3008 4673 882
Size: 617 1509 1665 2219
Fragments arranged by size:
2219 1665 1509 617
BsaAI yAC'GTr
Cuts at: 493 1204 3801 493
Size: 711 2597 2702
Fragments arranged by size:
2702 2597 711
BsaBI GATnn'nnATC
Cuts at: 6004 6004
Size: 6010
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2148 3817 4199 4834
Size: 53 83 186 936 615 1669 382 635
Cuts at: 4834 275
Size: 1451
Fragments arranged by size:
1669 1451 936 635 615 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4443 4841 5115 5262 5673 4443
Size: 398 274 147 411 4780
Fragments arranged by size:
4780 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2285 2315 1178
Size: 30 165 30 882 30 4873
Fragments arranged by size:
4873 882 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6010
BseYI C'CCAG_C
Cuts at: 2049 3163 5213 5709 2049
Size: 1114 2050 496 2350
Fragments arranged by size:
2350 2050 1114 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2673 2885 1163
Size: 573 937 212 4288
Fragments arranged by size:
4288 937 573 212
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3267 3460 4221 5249 1540
Size: 275 186 1266 193 761 1028 2301
Fragments arranged by size:
2301 1266 1028 761 275 193 186
BsiHKAI G_wGCw'C
Cuts at: 2628 2646 4071 4156 5227 2628
Size: 18 1425 85 1071 3411
Fragments arranged by size:
3411 1425 1071 85 18
BsmI GAATG_Cn'
Cuts at: 3521 3614 3521
Size: 93 5917
BsmBI CGTCTCn'nnnn_
Cuts at: 2139 2139
Size: 6010
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2264 2628 2634 2646 2907 3151 3329
Size: 399 474 364 6 12 261 244 178
Cuts at: 3329 4071 4156 5227 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 474 399 364 261 244
178 85 12 6
BspEI T'CCGG_A
Cuts at: 5673 5673
Size: 6010
BspHI T'CATG_A
Cuts at: 3899 5629 3899
Size: 1730 4280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2655 2702 3365 844
Size: 1811 47 663 3489
Fragments arranged by size:
3489 1811 663 47
BsrBI CCG'CTC
Cuts at: 1394 1823 3897 1394
Size: 429 2074 3507
Fragments arranged by size:
3507 2074 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4505 4679 65
Size: 1183 3257 174 1396
Fragments arranged by size:
3257 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3266 4654 4841 1700
Size: 110 1456 1388 187 2869
Fragments arranged by size:
2869 1456 1388 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6010
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6010
BssSI C'ACGA_G
Cuts at: 2891 4070 5082 2891
Size: 1179 1012 3819
Fragments arranged by size:
3819 1179 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6010
BstBI TT'CG_AA
Cuts at: 4814 4814
Size: 6010
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6010
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6010
BstYI r'GATC_y
Cuts at: 1972 2514 3340 3707 4092 4109 5561 5647 5659
Size: 542 826 367 385 17 1452 86 12
Cuts at: 5659 6005 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 826 542 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4519
Fragments arranged by size:
4519 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5271
Fragments arranged by size:
5271 683 56
BtsI GCAGTG_nn'
Cuts at: 2644 2656 3182 3526 4320 4340 2644
Size: 12 526 344 794 20 4314
Fragments arranged by size:
4314 794 526 344 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6010
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5975
DraI TTT'AAA
Cuts at: 3314 3314
Size: 6010
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2552 5017 809
Size: 507 1236 2465 1802
Fragments arranged by size:
2465 1802 1236 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2106 2378
Size: 54 1425 50 275 18 225 51 272
Cuts at: 2378 2936 3264 3457 4346 5975 8
Size: 558 328 193 889 1629 43
Fragments arranged by size:
1629 1425 889 558 328 275 272 225
193 54 51 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3264 3457 1537
Size: 275 1452 193 4090
Fragments arranged by size:
4090 1452 275 193
EarI CTCTTCn'nnn_
Cuts at: 1353 2863 3006 3940 1353
Size: 1510 143 934 3423
Fragments arranged by size:
3423 1510 934 143
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4597 4971 5117 387
Size: 1064 138 3008 374 146 1280
Fragments arranged by size:
3008 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2463 2921 2975 3129 4073 4670 5457
Size: 393 831 458 54 154 944 597 787
Cuts at: 5457 5725 1239
Size: 268 1524
Fragments arranged by size:
1524 944 831 787 597 458 393 268
154 54
EcoO109I rG'GnC_Cy
Cuts at: 2260 2261 2896 2260
Size: 1 635 5374
Fragments arranged by size:
5374 635 1
EcoRI G'AATT_C
Cuts at: 3319 3697 3319
Size: 378 5632
EcoRV GAT'ATC
Cuts at: 2071 2449 2740 2071
Size: 378 291 5341
Fragments arranged by size:
5341 378 291
FspI TGC'GCA
Cuts at: 2368 4516 2368
Size: 2148 3862
FspAI rTGC'GCAy
Cuts at: 2368 2368
Size: 6010
HaeII r_GCGC'y
Cuts at: 1678 3273 5157 1678
Size: 1595 1884 2531
Fragments arranged by size:
2531 1884 1595
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2438 2470 2594 2626 4651
Size: 32 135 32 866 32 124 32 2025
Cuts at: 4651 4683 4725 4757 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2025 866 135 124 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3238 3354 3600 669
Size: 988 281 1300 116 246 3079
Fragments arranged by size:
3079 1300 988 281 246 116
HindIII A'AGCT_T
Cuts at: 748 2111 748
Size: 1363 4647
HpaI GTT'AAC
Cuts at: 3600 3600
Size: 6010
KpnI G_GTAC'C
Cuts at: 3335 3335
Size: 6010
MfeI C'AATT_G
Cuts at: 3609 3609
Size: 6010
MluI A'CGCG_T
Cuts at: 5886 5886
Size: 6010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2098 2150 5124 5308 1305
Size: 793 52 2974 184 2007
Fragments arranged by size:
2974 2007 793 184 52
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2108 2380 10
Size: 54 1425 343 276 272 3640
Fragments arranged by size:
3640 1425 343 276 272 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2649 3245 3968 4327 4486 518
Size: 648 843 640 596 723 359 159 2042
Fragments arranged by size:
2042 843 723 648 640 596 359 159
MspA1I CmG'CkG
Cuts at: 1606 2803 3426 4103 5251 5496 1606
Size: 1197 623 677 1148 245 2120
Fragments arranged by size:
2120 1197 1148 677 623 245
NaeI GCC'GGC
Cuts at: 3268 3268
Size: 6010
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4519
Fragments arranged by size:
4519 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6010
NgoMIV G'CCGG_C
Cuts at: 3266 3266
Size: 6010
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6010
NotI GC'GGCC_GC
Cuts at: 3457 3457
Size: 6010
NspI r_CATG'y
Cuts at: 1132 1229 2280 3244 3368 4913 1132
Size: 97 1051 964 124 1545 2229
Fragments arranged by size:
2229 1545 1051 964 124 97
PasI CC'CwG_GG
Cuts at: 1348 2257 1348
Size: 909 5101
PciI A'CATG_T
Cuts at: 3240 4909 3240
Size: 1669 4341
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2966 1108
Size: 1858 4152
PfoI T'CCnGG_A
Cuts at: 2042 2952 2042
Size: 910 5100
PmeI GTTT'AAAC
Cuts at: 3314 3314
Size: 6010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4079 4111 5592 5624 4079
Size: 32 1481 32 4465
Fragments arranged by size:
4465 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2896 2896
Size: 6010
PshAI GACnn'nnGTC
Cuts at: 1613 2151 2166 1613
Size: 538 15 5457
Fragments arranged by size:
5457 538 15
PsiI TTA'TAA
Cuts at: 3580 3580
Size: 6010
PspOMI G'GGCC_C
Cuts at: 2260 2630 2260
Size: 370 5640
PstI C_TGCA'G
Cuts at: 830 2651 3362 830
Size: 1821 711 3478
Fragments arranged by size:
3478 1821 711
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6010
PvuII CAG'CTG
Cuts at: 2803 2803
Size: 6010
SalI G'TCGA_C
Cuts at: 1936 3352 1936
Size: 1416 4594
SbfI CC_TGCA'GG
Cuts at: 3362 3362
Size: 6010
ScaI AGT'ACT
Cuts at: 4258 5970 4258
Size: 1712 4298
SfcI C'TryA_G
Cuts at: 826 1045 2647 3283 3358 4493 5174 5365 826
Size: 219 1602 636 75 1135 681 191 1471
Fragments arranged by size:
1602 1471 1135 681 636 219 191 75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6010
SmaI CCC'GGG
Cuts at: 1872 3337 1872
Size: 1465 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2508 4114 5015 5277 820
Size: 197 926 565 1606 901 262 1553
Fragments arranged by size:
1606 1553 926 901 565 262 197
SnaBI TAC'GTA
Cuts at: 493 3801 493
Size: 3308 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6010
SphI G_CATG'C
Cuts at: 1132 3368 1132
Size: 2236 3774
SspI AAT'ATT
Cuts at: 5 52 3934 5
Size: 47 3882 2081
Fragments arranged by size:
3882 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6010
StyI C'CwwG_G
Cuts at: 513 1065 2004 3449 513
Size: 552 939 1445 3074
Fragments arranged by size:
3074 1445 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2956 2992 4037 4054 4207 4392 4837
Size: 1255 1376 36 1045 17 153 185 445
Cuts at: 4837 325
Size: 1498
Fragments arranged by size:
1498 1376 1255 1045 445 185 153 36
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4256 5968 96
Size: 274 80 33 51 1443 2279 1712 138
Fragments arranged by size:
2279 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2285 2317 5970 6002 2285
Size: 32 3653 32 2293
Fragments arranged by size:
3653 2293 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6010
XbaI T'CTAG_A
Cuts at: 3346 3346
Size: 6010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2845 2950 2997 2845
Size: 105 47 5858
Fragments arranged by size:
5858 105 47
XhoI C'TCGA_G
Cuts at: 1943 2508 1943
Size: 565 5445
XmaI C'CCGG_G
Cuts at: 1870 3335 1870
Size: 1465 4545
XmnI GAAnn'nnTTC
Cuts at: 3227 4139 3227
Size: 912 5098
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2149 3818 276
Size: 53 83 186 936 615 1669 2468
Fragments arranged by size:
2468 1669 936 615 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
FalI FseI KasI NarI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI