Restriction map for
pFN21AB5673
(Circular) (Six-base) MAPSORT of: pFN21AB5673.seq Check: 3608 from: 1 to: 6355
pFN21AB5673 6355 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4165 278
Size: 53 83 186 936 2629 2468
Fragments arranged by size:
2629 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2585 3698 1937
Size: 648 1113 4594
Fragments arranged by size:
4594 1113 648
Acc65I G'GTAC_C
Cuts at: 3676 3676
Size: 6355
AclI AA'CG_TT
Cuts at: 1563 4482 4855 1563
Size: 2919 373 3063
Fragments arranged by size:
3063 2919 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3058 4418 5802 6070 1632
Size: 1426 1360 1384 268 1917
Fragments arranged by size:
1917 1426 1384 1360 268
AfeI AGC'GCT
Cuts at: 3079 3079
Size: 6355
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6158
AflIII A'CryG_T
Cuts at: 1203 2433 3648 5254 6231 1203
Size: 1230 1215 1606 977 1327
Fragments arranged by size:
1606 1327 1230 1215 977
AgeI A'CCGG_T
Cuts at: 5186 5186
Size: 6355
AhdI GACnn_n'nnGTC
Cuts at: 5084 5084
Size: 6355
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6355
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6355
AlwNI CAG_nnn'CTG
Cuts at: 4098 5670 4098
Size: 1572 4783
ApaLI G'TGCA_C
Cuts at: 4412 5568 4412
Size: 1156 5199
ApoI r'AATT_y
Cuts at: 1493 2543 2842 3664 3890 4042 6151 1493
Size: 1050 299 822 226 152 2109 1697
Fragments arranged by size:
2109 1697 1050 822 299 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4909 160
Size: 872 3877 1606
Fragments arranged by size:
3877 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6355
AvaI C'yCGr_G
Cuts at: 1870 1943 2832 3680 1870
Size: 73 889 848 4545
Fragments arranged by size:
4545 889 848 73
BamHI G'GATC_C
Cuts at: 3685 4052 3685
Size: 367 5988
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3676 5131 618
Size: 325 217 627 1889 1455 1842
Fragments arranged by size:
1889 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3072 3674 1391
Size: 1681 602 4072
Fragments arranged by size:
4072 1681 602
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2082 928
Size: 971 183 5201
Fragments arranged by size:
5201 971 183
BbvCI CC'TCA_GC
Cuts at: 2465 2465
Size: 6355
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4546 4580 1903
Size: 34 2609 34 3678
Fragments arranged by size:
3678 2609 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4247 5457 4247
Size: 1210 5145
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6355
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4966 136
Size: 107 122 71 631 605 3294 1525
Fragments arranged by size:
3294 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6350 6350
Size: 6355
BlpI GC'TnA_GC
Cuts at: 3772 3772
Size: 6355
Bme1580I G_kGCm'C
Cuts at: 1790 3043 4416 5572 1790
Size: 1253 1373 1156 2573
Fragments arranged by size:
2573 1373 1253 1156
BmgBI CAC'GTC
Cuts at: 1318 3237 3578 1318
Size: 1919 341 4095
Fragments arranged by size:
4095 1919 341
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4086 5044 442
Size: 416 53 83 399 24 2669 958 1753
Fragments arranged by size:
2669 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6355
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2364 3563 2364
Size: 1199 5156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2334 5015 1239
Size: 1095 2681 2579
Fragments arranged by size:
2681 2579 1095
Bpu10I CC'TnA_GC
Cuts at: 2465 2850 2465
Size: 385 5970
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2531 3427 3546 4480 5345 5643 2531
Size: 896 119 934 865 298 3243
Fragments arranged by size:
3243 934 896 865 298 119
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5018 882
Size: 617 3519 2219
Fragments arranged by size:
3519 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3649 4146 493
Size: 711 2445 497 2702
Fragments arranged by size:
2702 2445 711 497
BsaBI GATnn'nnATC
Cuts at: 3337 6349 3337
Size: 3012 3343
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4162 4544 5179 275
Size: 53 83 186 936 2629 382 635 1451
Fragments arranged by size:
2629 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4788 5186 5460 5607 6018 4788
Size: 398 274 147 411 5125
Fragments arranged by size:
5125 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6130
Fragments arranged by size:
6130 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2438 1186
Size: 1252 5103
BseYI C'CCAG_C
Cuts at: 5558 6054 5558
Size: 496 5859
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5782
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3805 4566 5594 1540
Size: 275 186 1804 761 1028 2301
Fragments arranged by size:
2301 1804 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2347 3429 3438 4416 4501 5572 2347
Size: 1082 9 978 85 1071 3130
Fragments arranged by size:
3130 1082 1071 978 85 9
BsmI GAATG_Cn'
Cuts at: 3866 3959 3866
Size: 93 6262
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2347 3043 3072 3429 3438 3674 4416
Size: 399 557 696 29 357 9 236 742
Cuts at: 4416 4501 5572 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 696 557 399 357 236
85 29 9
BspEI T'CCGG_A
Cuts at: 6018 6018
Size: 6355
BspHI T'CATG_A
Cuts at: 2685 4244 5974 2685
Size: 1559 1730 3066
Fragments arranged by size:
3066 1730 1559
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3710 844
Size: 2866 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4242 1394
Size: 429 2419 3507
Fragments arranged by size:
3507 2419 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3541 4850 5024 65
Size: 1183 2293 1309 174 1396
Fragments arranged by size:
2293 1396 1309 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2037 4999 5186 1700
Size: 110 227 2962 187 2869
Fragments arranged by size:
2962 2869 227 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6355
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6355
BssSI C'ACGA_G
Cuts at: 3081 4415 5427 3081
Size: 1334 1012 4009
Fragments arranged by size:
4009 1334 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6355
BstBI TT'CG_AA
Cuts at: 5159 5159
Size: 6355
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6355
BstXI CCAn_nnnn'nTGG
Cuts at: 3610 3633 3610
Size: 23 6332
BstYI r'GATC_y
Cuts at: 1972 3685 4052 4437 4454 5906 5992 6004 6350
Size: 1713 367 385 17 1452 86 12 346
Cuts at: 6350 1972
Size: 1977
Fragments arranged by size:
1977 1713 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2064 2064
Size: 6355
BtgI C'CryG_G
Cuts at: 513 1065 2004 2671 513
Size: 552 939 667 4197
Fragments arranged by size:
4197 939 667 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5616
Fragments arranged by size:
5616 683 56
BtsI GCAGTG_nn'
Cuts at: 2744 3160 3871 4665 4685 2744
Size: 416 711 794 20 4414
Fragments arranged by size:
4414 794 711 416 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6355
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6320
DraI TTT'AAA
Cuts at: 3659 3659
Size: 6355
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5362 809
Size: 507 4046 1802
Fragments arranged by size:
4046 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3623 3802 4691
Size: 54 1425 50 275 18 1793 179 889
Cuts at: 4691 6320 8
Size: 1629 43
Fragments arranged by size:
1793 1629 1425 889 275 179 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3802 1537
Size: 275 1990 4090
Fragments arranged by size:
4090 1990 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2010 2672 3140 3434 3443 4285 1353
Size: 657 662 468 294 9 842 3423
Fragments arranged by size:
3423 842 662 657 468 294 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4942 5316 5462 387
Size: 1064 138 3353 374 146 1280
Fragments arranged by size:
3353 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2334 3058 4418 5015 5802 6070 1239
Size: 393 702 724 1360 597 787 268 1524
Fragments arranged by size:
1524 1360 787 724 702 597 393 268
EcoRI G'AATT_C
Cuts at: 3664 4042 3664
Size: 378 5977
FspI TGC'GCA
Cuts at: 4861 4861
Size: 6355
HaeII r_GCGC'y
Cuts at: 1678 2383 3081 5502 1678
Size: 705 698 2421 2531
Fragments arranged by size:
2531 2421 705 698
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2009 2041 2632 2647 2664
Size: 32 135 32 437 32 591 15 17
Cuts at: 2664 2679 4996 5028 5070 5102 1373
Size: 15 2317 32 42 32 2626
Fragments arranged by size:
2626 2317 591 437 135 42 32 32
32 32 32 17 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2166 2887 3699 3945 669
Size: 988 281 228 721 812 246 3079
Fragments arranged by size:
3079 988 812 721 281 246 228
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6355
HpaI GTT'AAC
Cuts at: 3945 3945
Size: 6355
KpnI G_GTAC'C
Cuts at: 3680 3680
Size: 6355
MfeI C'AATT_G
Cuts at: 3954 3954
Size: 6355
MluI A'CGCG_T
Cuts at: 6231 6231
Size: 6355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3270 3464 5469 5653 1305
Size: 1965 194 2005 184 2007
Fragments arranged by size:
2007 2005 1965 194 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4533
Fragments arranged by size:
4533 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2223 3387 4313 4672 4831 518
Size: 648 1057 1164 926 359 159 2042
Fragments arranged by size:
2042 1164 1057 926 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2653 3771 4448 5596 5841 1606
Size: 1047 1118 677 1148 245 2120
Fragments arranged by size:
2120 1148 1118 1047 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2671 513
Size: 552 939 667 4197
Fragments arranged by size:
4197 939 667 552
NdeI CA'TA_TG
Cuts at: 387 2725 387
Size: 2338 4017
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6355
NotI GC'GGCC_GC
Cuts at: 3802 3802
Size: 6355
NspI r_CATG'y
Cuts at: 1132 1229 2437 3713 5258 1132
Size: 97 1208 1276 1545 2229
Fragments arranged by size:
2229 1545 1276 1208 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6355
PciI A'CATG_T
Cuts at: 2433 5254 2433
Size: 2821 3534
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6355
PfoI T'CCnGG_A
Cuts at: 3632 3632
Size: 6355
PmeI GTTT'AAAC
Cuts at: 3659 3659
Size: 6355
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4424 4456 5937 5969 4424
Size: 32 1481 32 4810
Fragments arranged by size:
4810 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6355
PsiI TTA'TAA
Cuts at: 3925 3925
Size: 6355
PstI C_TGCA'G
Cuts at: 830 3707 830
Size: 2877 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6355
SalI G'TCGA_C
Cuts at: 1936 3697 1936
Size: 1761 4594
SapI GCTCTTCn'nnn_
Cuts at: 3434 3443 3434
Size: 9 6346
SbfI CC_TGCA'GG
Cuts at: 3707 3707
Size: 6355
ScaI AGT'ACT
Cuts at: 4603 6315 4603
Size: 1712 4643
SexAI A'CCwGG_T
Cuts at: 2564 2564
Size: 6355
SfcI C'TryA_G
Cuts at: 826 1045 2632 2880 3375 3703 4838 5519 5710
Size: 219 1587 248 495 328 1135 681 191
Cuts at: 5710 826
Size: 1471
Fragments arranged by size:
1587 1471 1135 681 495 328 248 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6355
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6355
SmaI CCC'GGG
Cuts at: 1872 3682 1872
Size: 1810 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2510 3406 3561 4459 5360 5622
Size: 197 926 567 896 155 898 901 262
Cuts at: 5622 820
Size: 1553
Fragments arranged by size:
1553 926 901 898 896 567 262 197
155
SnaBI TAC'GTA
Cuts at: 493 4146 493
Size: 3653 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6355
SphI G_CATG'C
Cuts at: 1132 3713 1132
Size: 2581 3774
SspI AAT'ATT
Cuts at: 5 52 4279 5
Size: 47 4227 2081
Fragments arranged by size:
4227 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6355
StyI C'CwwG_G
Cuts at: 513 1065 2004 2671 3393 3794 513
Size: 552 939 667 722 401 3074
Fragments arranged by size:
3074 939 722 667 552 401
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2000 2265 4382 4399 4552 4737 5182
Size: 1255 420 265 2117 17 153 185 445
Cuts at: 5182 325
Size: 1498
Fragments arranged by size:
2117 1498 1255 445 420 265 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2898 3015 3379
Size: 274 80 33 51 1443 921 117 364
Cuts at: 3379 4601 6313 96
Size: 1222 1712 138
Fragments arranged by size:
1712 1443 1222 921 364 274 138 117
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2490 2522 6315 6347 2490
Size: 32 3793 32 2498
Fragments arranged by size:
3793 2498 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6355
XbaI T'CTAG_A
Cuts at: 3691 3691
Size: 6355
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6355
XmaI C'CCGG_G
Cuts at: 1870 3680 1870
Size: 1810 4545
XmnI GAAnn'nnTTC
Cuts at: 4484 4484
Size: 6355
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4163 276
Size: 53 83 186 936 2629 2468
Fragments arranged by size:
2629 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI
XcmI