Restriction map for
pFN21AB5672
(Circular) (Six-base) MAPSORT of: pFN21AB5672.seq Check: 145 from: 1 to: 6313
pFN21AB5672 6313 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4123 278
Size: 53 83 186 936 2587 2468
Fragments arranged by size:
2587 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3656 1937
Size: 1719 4594
Acc65I G'GTAC_C
Cuts at: 3249 3634 3249
Size: 385 5928
AclI AA'CG_TT
Cuts at: 1563 4440 4813 1563
Size: 2877 373 3063
Fragments arranged by size:
3063 2877 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2097 2423 2773 3149 4376 5760 6028 1632
Size: 465 326 350 376 1227 1384 268 1917
Fragments arranged by size:
1917 1384 1227 465 376 350 326 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6116
AflIII A'CryG_T
Cuts at: 1203 5212 6189 1203
Size: 4009 977 1327
Fragments arranged by size:
4009 1327 977
AgeI A'CCGG_T
Cuts at: 5144 5144
Size: 6313
AhdI GACnn_n'nnGTC
Cuts at: 5042 5042
Size: 6313
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6313
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2836 1184
Size: 1652 4661
AlwNI CAG_nnn'CTG
Cuts at: 2507 4056 5628 2507
Size: 1549 1572 3192
Fragments arranged by size:
3192 1572 1549
ApaI G_GGCC'C
Cuts at: 2704 2830 2704
Size: 126 6187
ApaLI G'TGCA_C
Cuts at: 4370 5526 4370
Size: 1156 5157
ApoI r'AATT_y
Cuts at: 1493 2312 3622 3848 4000 6109 1493
Size: 819 1310 226 152 2109 1697
Fragments arranged by size:
2109 1697 1310 819 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2172 4867 160
Size: 872 1140 2695 1606
Fragments arranged by size:
2695 1606 1140 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6313
AvaI C'yCGr_G
Cuts at: 1870 1943 3638 1870
Size: 73 1695 4545
Fragments arranged by size:
4545 1695 73
BamHI G'GATC_C
Cuts at: 3643 4010 3643
Size: 367 5946
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3249 3634 5089 618
Size: 325 217 627 1462 385 1455 1842
Fragments arranged by size:
1842 1462 1455 627 385 325 217
BanII G_rGCy'C
Cuts at: 1391 2024 2225 2449 2704 2830 3112 3632 1391
Size: 633 201 224 255 126 282 520 4072
Fragments arranged by size:
4072 633 520 282 255 224 201 126
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2192 3525 928
Size: 971 293 1333 3716
Fragments arranged by size:
3716 1333 971 293
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3298 3332 4504 4538 1903
Size: 34 1361 34 1172 34 3678
Fragments arranged by size:
3678 1361 1172 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4205 5415 4205
Size: 1210 5103
BclI T'GATC_A
Cuts at: 1548 2244 1548
Size: 696 5617
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4924 136
Size: 107 122 71 631 605 3252 1525
Fragments arranged by size:
3252 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3471 6308 3471
Size: 2837 3476
BlpI GC'TnA_GC
Cuts at: 3730 3730
Size: 6313
Bme1580I G_kGCm'C
Cuts at: 1790 2575 2704 2830 4374 5530 1790
Size: 785 129 126 1544 1156 2573
Fragments arranged by size:
2573 1544 1156 785 129 126
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6313
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2241 2585 2982
Size: 416 53 83 399 24 824 344 397
Cuts at: 2982 3272 4044 5002 442
Size: 290 772 958 1753
Fragments arranged by size:
1753 958 824 772 416 399 397 344
290 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2610 3359 3434 4973 1239
Size: 1371 749 75 1539 2579
Fragments arranged by size:
2579 1539 1371 749 75
Bpu10I CC'TnA_GC
Cuts at: 3237 3385 3237
Size: 148 6165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4438 5303 5601 4438
Size: 865 298 5150
Fragments arranged by size:
5150 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2003 2758 3262 4976 882
Size: 617 504 755 504 1714 2219
Fragments arranged by size:
2219 1714 755 617 504 504
BsaAI yAC'GTr
Cuts at: 493 1204 4104 493
Size: 711 2900 2702
Fragments arranged by size:
2900 2702 711
BsaBI GATnn'nnATC
Cuts at: 3158 6307 3158
Size: 3149 3164
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4120 4502 5137 275
Size: 53 83 186 936 2587 382 635 1451
Fragments arranged by size:
2587 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2118 2316 2882 3060 4746 5144 5418 5565 5976
Size: 198 566 178 1686 398 274 147 411
Cuts at: 5976 2118
Size: 2455
Fragments arranged by size:
2455 1686 566 411 398 274 198 178
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6088
Fragments arranged by size:
6088 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6313
BseYI C'CCAG_C
Cuts at: 2823 5516 6012 2823
Size: 2693 496 3124
Fragments arranged by size:
3124 2693 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5740
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3763 4524 5552 1540
Size: 275 186 1762 761 1028 2301
Fragments arranged by size:
2301 1762 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2225 3504 4374 4459 5530 2225
Size: 1279 870 85 1071 3008
Fragments arranged by size:
3008 1279 1071 870 85
BsmI GAATG_Cn'
Cuts at: 2109 2695 3824 3917 2109
Size: 586 1129 93 4505
Fragments arranged by size:
4505 1129 586 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2080 2080
Size: 6313
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2225 2449 2575 2704 2830 3112
Size: 399 234 201 224 126 129 126 282
Cuts at: 3112 3504 3632 4374 4459 5530 1391
Size: 392 128 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 392 282 234 224
201 129 128 126 126 85
BspEI T'CCGG_A
Cuts at: 2118 2316 2882 3060 5976 2118
Size: 198 566 178 2916 2455
Fragments arranged by size:
2916 2455 566 198 178
BspHI T'CATG_A
Cuts at: 4202 5932 4202
Size: 1730 4583
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2298 3668 844
Size: 1454 1370 3489
Fragments arranged by size:
3489 1454 1370
BsrBI CCG'CTC
Cuts at: 1394 1823 4200 1394
Size: 429 2377 3507
Fragments arranged by size:
3507 2377 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4808 4982 65
Size: 1183 3560 174 1396
Fragments arranged by size:
3560 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4957 5144 1700
Size: 110 3147 187 2869
Fragments arranged by size:
3147 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2420 96
Size: 2324 3989
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6313
BssSI C'ACGA_G
Cuts at: 3458 4373 5385 3458
Size: 915 1012 4386
Fragments arranged by size:
4386 1012 915
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6313
BstBI TT'CG_AA
Cuts at: 3308 5117 3308
Size: 1809 4504
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6313
BstYI r'GATC_y
Cuts at: 1972 2862 3471 3643 4010 4395 4412 5864 5950
Size: 890 609 172 367 385 17 1452 86
Cuts at: 5950 5962 6308 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 890 609 385 367 346 172
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3585 3585
Size: 6313
BtgI C'CryG_G
Cuts at: 513 1065 2004 2013 2955 3594 513
Size: 552 939 9 942 639 3232
Fragments arranged by size:
3232 942 939 639 552 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5574
Fragments arranged by size:
5574 683 56
BtsI GCAGTG_nn'
Cuts at: 3385 3829 4623 4643 3385
Size: 444 794 20 5055
Fragments arranged by size:
5055 794 444 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6313
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2361 2396 3361 3396 529
Size: 35 1797 35 965 35 3446
Fragments arranged by size:
3446 1797 965 35 35 35
DraI TTT'AAA
Cuts at: 2149 3617 2149
Size: 1468 4845
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2885 5320 809
Size: 507 1569 2435 1802
Fragments arranged by size:
2435 1802 1569 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3760 4649 6278
Size: 54 1425 50 275 18 1930 889 1629
Cuts at: 6278 8
Size: 43
Fragments arranged by size:
1930 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3760 1537
Size: 275 1948 4090
Fragments arranged by size:
4090 1948 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2042 2089 2097 2399 2792 4243 1353
Size: 689 47 8 302 393 1451 3423
Fragments arranged by size:
3423 1451 689 393 302 47 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4900 5274 5420 387
Size: 1064 138 3311 374 146 1280
Fragments arranged by size:
3311 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2223 2223
Size: 6313
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2097 2423 2610 2773 3149 3359 3434
Size: 393 465 326 187 163 376 210 75
Cuts at: 3434 4376 4973 5760 6028 1239
Size: 942 597 787 268 1524
Fragments arranged by size:
1524 942 787 597 465 393 376 326
268 210 187 163 75
EcoNI CCTnn'n_nnAGG
Cuts at: 3413 3413
Size: 6313
EcoO109I rG'GnC_Cy
Cuts at: 2701 2827 3589 2701
Size: 126 762 5425
Fragments arranged by size:
5425 762 126
EcoRI G'AATT_C
Cuts at: 3622 4000 3622
Size: 378 5935
FspI TGC'GCA
Cuts at: 4819 4819
Size: 6313
HaeII r_GCGC'y
Cuts at: 1678 5460 1678
Size: 3782 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2601 2633 4954 4986 5028
Size: 32 135 32 1029 32 2321 32 42
Cuts at: 5028 5060 1373
Size: 32 2626
Fragments arranged by size:
2626 2321 1029 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2649 3657 3903 669
Size: 988 281 711 1008 246 3079
Fragments arranged by size:
3079 1008 988 711 281 246
HindIII A'AGCT_T
Cuts at: 748 3069 748
Size: 2321 3992
HpaI GTT'AAC
Cuts at: 2649 3903 2649
Size: 1254 5059
KpnI G_GTAC'C
Cuts at: 3253 3638 3253
Size: 385 5928
MfeI C'AATT_G
Cuts at: 3912 3912
Size: 6313
MluI A'CGCG_T
Cuts at: 6189 6189
Size: 6313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5427 5611 1305
Size: 4122 184 2007
Fragments arranged by size:
4122 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4491
Fragments arranged by size:
4491 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4271 4630 4789 518
Size: 648 3105 359 159 2042
Fragments arranged by size:
3105 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3729 4406 5554 5799 1606
Size: 2123 677 1148 245 2120
Fragments arranged by size:
2123 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2013 3594 513
Size: 552 939 9 1581 3232
Fragments arranged by size:
3232 1581 939 552 9
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6313
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6313
NotI GC'GGCC_GC
Cuts at: 3760 3760
Size: 6313
NspI r_CATG'y
Cuts at: 1132 1229 3671 5216 1132
Size: 97 2442 1545 2229
Fragments arranged by size:
2442 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2024 1348
Size: 676 5637
PciI A'CATG_T
Cuts at: 5212 5212
Size: 6313
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6313
PmeI GTTT'AAAC
Cuts at: 3617 3617
Size: 6313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4382 4414 5895 5927 4382
Size: 32 1481 32 4768
Fragments arranged by size:
4768 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2993 1613
Size: 1380 4933
PsiI TTA'TAA
Cuts at: 3883 3883
Size: 6313
PspOMI G'GGCC_C
Cuts at: 2700 2826 2700
Size: 126 6187
PstI C_TGCA'G
Cuts at: 830 2295 3665 830
Size: 1465 1370 3478
Fragments arranged by size:
3478 1465 1370
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6313
SacI G_AGCT'C
Cuts at: 2225 2225
Size: 6313
SalI G'TCGA_C
Cuts at: 1936 3655 1936
Size: 1719 4594
SapI GCTCTTCn'nnn_
Cuts at: 2042 2042
Size: 6313
SbfI CC_TGCA'GG
Cuts at: 3665 3665
Size: 6313
ScaI AGT'ACT
Cuts at: 4561 6273 4561
Size: 1712 4601
SexAI A'CCwGG_T
Cuts at: 3252 3252
Size: 6313
SfcI C'TryA_G
Cuts at: 826 1045 2291 3661 4796 5477 5668 826
Size: 219 1246 1370 1135 681 191 1471
Fragments arranged by size:
1471 1370 1246 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6313
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6313
SmaI CCC'GGG
Cuts at: 1872 3640 1872
Size: 1768 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4417 5318 5580 820
Size: 197 926 2474 901 262 1553
Fragments arranged by size:
2474 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4104 493
Size: 3611 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6313
SphI G_CATG'C
Cuts at: 1132 3671 1132
Size: 2539 3774
SspI AAT'ATT
Cuts at: 5 52 2458 3091 4237 5
Size: 47 2406 633 1146 2081
Fragments arranged by size:
2406 2081 1146 633 47
StuI AGG'CCT
Cuts at: 1517 3485 1517
Size: 1968 4345
StyI C'CwwG_G
Cuts at: 513 1065 2004 2013 3048 3594 3752 513
Size: 552 939 9 1035 546 158 3074
Fragments arranged by size:
3074 1035 939 552 546 158 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4340 4357 4510 4695 5140 325
Size: 1255 2760 17 153 185 445 1498
Fragments arranged by size:
2760 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2420 2770 4559
Size: 274 80 33 51 1443 443 350 1789
Cuts at: 4559 6271 96
Size: 1712 138
Fragments arranged by size:
1789 1712 1443 443 350 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2298 2330 6273 6305 2298
Size: 32 3943 32 2306
Fragments arranged by size:
3943 2306 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6313
XbaI T'CTAG_A
Cuts at: 3649 3649
Size: 6313
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6313
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6313
XmaI C'CCGG_G
Cuts at: 1870 3638 1870
Size: 1768 4545
XmnI GAAnn'nnTTC
Cuts at: 3162 3485 4442 3162
Size: 323 957 5033
Fragments arranged by size:
5033 957 323
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4121 276
Size: 53 83 186 936 2587 2468
Fragments arranged by size:
2587 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII
SacII SanDI SfiI SfoI SrfI SwaI