Restriction map for
pFN21AB5670
(Circular) (Six-base) MAPSORT of: pFN21AB5670.seq Check: 8116 from: 1 to: 7480
pFN21AB5670 7480 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2444 2444
Size: 7480
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5290 278
Size: 53 83 186 936 3754 2468
Fragments arranged by size:
3754 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4537 4823 1937
Size: 2600 286 4594
Fragments arranged by size:
4594 2600 286
Acc65I G'GTAC_C
Cuts at: 4801 4801
Size: 7480
AclI AA'CG_TT
Cuts at: 1563 2329 5607 5980 1563
Size: 766 3278 373 3063
Fragments arranged by size:
3278 3063 766 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2676 3183 3700 4292 5543 6927 7195 1632
Size: 1044 507 517 592 1251 1384 268 1917
Fragments arranged by size:
1917 1384 1251 1044 592 517 507 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7283
AflIII A'CryG_T
Cuts at: 1203 4266 6379 7356 1203
Size: 3063 2113 977 1327
Fragments arranged by size:
3063 2113 1327 977
AgeI A'CCGG_T
Cuts at: 6311 6311
Size: 7480
AhdI GACnn_n'nnGTC
Cuts at: 6209 6209
Size: 7480
AleI CACnn'nnGTG
Cuts at: 1166 4039 1166
Size: 2873 4607
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7480
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2195 2227 2195
Size: 32 7448
AlwNI CAG_nnn'CTG
Cuts at: 4103 4714 5223 6795 4103
Size: 611 509 1572 4788
Fragments arranged by size:
4788 1572 611 509
ApaI G_GGCC'C
Cuts at: 2153 2153
Size: 7480
ApaLI G'TGCA_C
Cuts at: 5537 6693 5537
Size: 1156 6324
ApoI r'AATT_y
Cuts at: 1493 2342 2418 2677 2855 3413 3498 3512 4367
Size: 849 76 259 178 558 85 14 855
Cuts at: 4367 4789 5015 5167 7276 1493
Size: 422 226 152 2109 1697
Fragments arranged by size:
2109 1697 855 849 558 422 259 226
178 152 85 76 14
AseI AT'TA_AT
Cuts at: 160 1032 2400 6034 160
Size: 872 1368 3634 1606
Fragments arranged by size:
3634 1606 1368 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7480
AvaI C'yCGr_G
Cuts at: 1870 1943 4805 1870
Size: 73 2862 4545
Fragments arranged by size:
4545 2862 73
BamHI G'GATC_C
Cuts at: 3811 4810 5177 3811
Size: 999 367 6114
Fragments arranged by size:
6114 999 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4801 6256 618
Size: 325 217 627 3014 1455 1842
Fragments arranged by size:
3014 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2153 2196 4799 1391
Size: 762 43 2603 4072
Fragments arranged by size:
4072 2603 762 43
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2063 3846 928
Size: 971 164 1783 4562
Fragments arranged by size:
4562 1783 971 164
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3463 3497 4590 4624 5671 5705 1903
Size: 34 1526 34 1093 34 1047 34 3678
Fragments arranged by size:
3678 1526 1093 1047 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5372 6582 5372
Size: 1210 6270
BclI T'GATC_A
Cuts at: 1548 3159 1548
Size: 1611 5869
BfrBI ATG'CAT
Cuts at: 3564 4055 3564
Size: 491 6989
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6091 136
Size: 107 122 71 631 605 4419 1525
Fragments arranged by size:
4419 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2599 3757 7475 2599
Size: 1158 3718 2604
Fragments arranged by size:
3718 2604 1158
BlpI GC'TnA_GC
Cuts at: 3432 4897 3432
Size: 1465 6015
Bme1580I G_kGCm'C
Cuts at: 1790 2153 4600 5541 6697 1790
Size: 363 2447 941 1156 2573
Fragments arranged by size:
2573 2447 1156 941 363
BmgBI CAC'GTC
Cuts at: 1318 3995 1318
Size: 2677 4803
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2900 2996 4630
Size: 416 53 83 399 24 1483 96 1634
Cuts at: 4630 5211 6169 442
Size: 581 958 1753
Fragments arranged by size:
1753 1634 1483 958 581 416 399 96
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7480
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2180 3000 3362 3371 4722 6140 1239
Size: 941 820 362 9 1351 1418 2579
Fragments arranged by size:
2579 1418 1351 941 820 362 9
Bpu10I CC'TnA_GC
Cuts at: 2081 2081
Size: 7480
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2162 2624 5605 6470 6768 2162
Size: 462 2981 865 298 2874
Fragments arranged by size:
2981 2874 865 462 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6143 882
Size: 617 4644 2219
Fragments arranged by size:
4644 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5271 493
Size: 711 4067 2702
Fragments arranged by size:
4067 2702 711
BsaBI GATnn'nnATC
Cuts at: 7474 7474
Size: 7480
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5287 5669 6304 275
Size: 53 83 186 936 3754 382 635 1451
Fragments arranged by size:
3754 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2052 5913 6311 6585 6732 7143 2052
Size: 3861 398 274 147 411 2389
Fragments arranged by size:
3861 2389 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7255
Fragments arranged by size:
7255 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2321 1186
Size: 1135 6345
BseYI C'CCAG_C
Cuts at: 3815 6683 7179 3815
Size: 2868 496 4116
Fragments arranged by size:
4116 2868 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2138 2478 1163
Size: 573 402 340 6165
Fragments arranged by size:
6165 573 402 340
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4930 5691 6719 1540
Size: 275 186 2929 761 1028 2301
Fragments arranged by size:
2929 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2196 2797 3919 5541 5626 6697 2196
Size: 601 1122 1622 85 1071 2979
Fragments arranged by size:
2979 1622 1122 1071 601 85
BsmI GAATG_Cn'
Cuts at: 2480 2663 3128 4055 4493 4529 4991 5084 2480
Size: 183 465 927 438 36 462 93 4876
Fragments arranged by size:
4876 927 465 462 438 183 93 36
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2153 2196 2797 3919 4600 4799 5541
Size: 399 363 43 601 1122 681 199 742
Cuts at: 5541 5626 6697 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1122 1071 742 681 601 399 363
199 85 43
BspEI T'CCGG_A
Cuts at: 2052 7143 2052
Size: 5091 2389
BspHI T'CATG_A
Cuts at: 4360 5369 7099 4360
Size: 1009 1730 4741
Fragments arranged by size:
4741 1730 1009
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2234 2444 4835 844
Size: 1390 210 2391 3489
Fragments arranged by size:
3489 2391 1390 210
BsrBI CCG'CTC
Cuts at: 1394 1823 5367 1394
Size: 429 3544 3507
Fragments arranged by size:
3544 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3727 3826 4119 4168 5975 6149 65
Size: 1183 2479 99 293 49 1807 174 1396
Fragments arranged by size:
2479 1807 1396 1183 293 174 99 49
BsrFI r'CCGG_y
Cuts at: 1700 1810 6124 6311 1700
Size: 110 4314 187 2869
Fragments arranged by size:
4314 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2715 4269 96
Size: 2619 1554 3307
Fragments arranged by size:
3307 2619 1554
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7480
BssSI C'ACGA_G
Cuts at: 5540 6552 5540
Size: 1012 6468
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4440 794
Size: 3646 3834
BstBI TT'CG_AA
Cuts at: 6284 6284
Size: 7480
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7480
BstXI CCAn_nnnn'nTGG
Cuts at: 3221 3221
Size: 7480
BstYI r'GATC_y
Cuts at: 1972 2599 2650 2901 3194 3757 3811 4810 5177
Size: 627 51 251 293 563 54 999 367
Cuts at: 5177 5562 5579 7031 7117 7129 7475 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 999 627 563 385 367 346
293 251 86 54 51 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2215 513
Size: 552 1150 5778
Fragments arranged by size:
5778 1150 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6741
Fragments arranged by size:
6741 683 56
BtsI GCAGTG_nn'
Cuts at: 2825 3206 4996 5790 5810 2825
Size: 381 1790 794 20 4495
Fragments arranged by size:
4495 1790 794 381 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7480
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4048 4081 4083 4116 529
Size: 35 3484 33 2 33 3893
Fragments arranged by size:
3893 3484 35 33 33 2
DraI TTT'AAA
Cuts at: 2325 2347 2728 3418 3933 4784 2325
Size: 22 381 690 515 851 5021
Fragments arranged by size:
5021 851 690 515 381 22
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6487 809
Size: 507 5171 1802
Fragments arranged by size:
5171 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4927 5816 7445
Size: 54 1425 50 275 18 3097 889 1629
Cuts at: 7445 8
Size: 43
Fragments arranged by size:
3097 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4927 1537
Size: 275 3115 4090
Fragments arranged by size:
4090 3115 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2106 3588 3858 4192 5410 1353
Size: 753 1482 270 334 1218 3423
Fragments arranged by size:
3423 1482 1218 753 334 270
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6067 6441 6587 387
Size: 1064 138 4478 374 146 1280
Fragments arranged by size:
4478 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2194 2194
Size: 7480
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2180 2676 3000 3183 3362 3371 3700
Size: 393 548 496 324 183 179 9 329
Cuts at: 3700 4292 4722 5543 6140 6927 7195 1239
Size: 592 430 821 597 787 268 1524
Fragments arranged by size:
1524 821 787 597 592 548 496 430
393 329 324 268 183 179 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2101 3071 2101
Size: 970 6510
EcoO109I rG'GnC_Cy
Cuts at: 2149 2149
Size: 7480
EcoRI G'AATT_C
Cuts at: 2677 4789 5167 2677
Size: 2112 378 4990
Fragments arranged by size:
4990 2112 378
EcoRV GAT'ATC
Cuts at: 2371 2371
Size: 7480
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4543 4543
Size: 7480
FspI TGC'GCA
Cuts at: 5986 5986
Size: 7480
HaeII r_GCGC'y
Cuts at: 1678 6627 1678
Size: 4949 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 6121 6153 6195 6227 1373
Size: 32 135 32 4549 32 42 32 2626
Fragments arranged by size:
4549 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4824 5070 669
Size: 988 281 2886 246 3079
Fragments arranged by size:
3079 2886 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7480
HpaI GTT'AAC
Cuts at: 5070 5070
Size: 7480
KpnI G_GTAC'C
Cuts at: 4805 4805
Size: 7480
MfeI C'AATT_G
Cuts at: 5079 5079
Size: 7480
MluI A'CGCG_T
Cuts at: 7356 7356
Size: 7480
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2169 2283 6594 6778 1305
Size: 864 114 4311 184 2007
Fragments arranged by size:
4311 2007 864 184 114
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5658
Fragments arranged by size:
5658 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4019 4039 4172 5438 5797 5956 518
Size: 648 2853 20 133 1266 359 159 2042
Fragments arranged by size:
2853 2042 1266 648 359 159 133 20
MspA1I CmG'CkG
Cuts at: 1606 2990 3436 4466 4896 5573 6721 6966 1606
Size: 1384 446 1030 430 677 1148 245 2120
Fragments arranged by size:
2120 1384 1148 1030 677 446 430 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6928
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7480
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7480
NotI GC'GGCC_GC
Cuts at: 4927 4927
Size: 7480
NsiI A_TGCA'T
Cuts at: 3566 4057 3566
Size: 491 6989
NspI r_CATG'y
Cuts at: 1132 1229 3564 3677 4270 4458 4838 6383 1132
Size: 97 2335 113 593 188 380 1545 2229
Fragments arranged by size:
2335 2229 1545 593 380 188 113 97
PacI TTA_AT'TAA
Cuts at: 2400 2400
Size: 7480
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7480
PciI A'CATG_T
Cuts at: 4266 6379 4266
Size: 2113 5367
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7480
PfoI T'CCnGG_A
Cuts at: 2040 2196 2040
Size: 156 7324
PmeI GTTT'AAAC
Cuts at: 4784 4784
Size: 7480
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5549 5581 7062 7094 5549
Size: 32 1481 32 5935
Fragments arranged by size:
5935 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7480
PsiI TTA'TAA
Cuts at: 3366 4664 5050 3366
Size: 1298 386 5796
Fragments arranged by size:
5796 1298 386
PspOMI G'GGCC_C
Cuts at: 2149 2149
Size: 7480
PstI C_TGCA'G
Cuts at: 830 3789 4436 4613 4832 830
Size: 2959 647 177 219 3478
Fragments arranged by size:
3478 2959 647 219 177
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7480
PvuII CAG'CTG
Cuts at: 2990 3436 4466 2990
Size: 446 1030 6004
Fragments arranged by size:
6004 1030 446
SacI G_AGCT'C
Cuts at: 2196 2196
Size: 7480
SalI G'TCGA_C
Cuts at: 1936 4822 1936
Size: 2886 4594
SapI GCTCTTCn'nnn_
Cuts at: 4192 4192
Size: 7480
SbfI CC_TGCA'GG
Cuts at: 4832 4832
Size: 7480
ScaI AGT'ACT
Cuts at: 5728 7440 5728
Size: 1712 5768
SfcI C'TryA_G
Cuts at: 826 1045 2423 2799 3785 4432 4609 4828 5963
Size: 219 1378 376 986 647 177 219 1135
Cuts at: 5963 6644 6835 826
Size: 681 191 1471
Fragments arranged by size:
1471 1378 1135 986 681 647 376 219
219 191 177
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7480
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7480
SmaI CCC'GGG
Cuts at: 1872 4807 1872
Size: 2935 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2177 2603 5584 6485 6747 820
Size: 197 926 234 426 2981 901 262 1553
Fragments arranged by size:
2981 1553 926 901 426 262 234 197
SnaBI TAC'GTA
Cuts at: 493 5271 493
Size: 4778 2702
SpeI A'CTAG_T
Cuts at: 152 3134 4229 152
Size: 2982 1095 3403
Fragments arranged by size:
3403 2982 1095
SphI G_CATG'C
Cuts at: 1132 3564 3677 4838 1132
Size: 2432 113 1161 3774
Fragments arranged by size:
3774 2432 1161 113
SspI AAT'ATT
Cuts at: 5 52 4081 5404 5
Size: 47 4029 1323 2081
Fragments arranged by size:
4029 2081 1323 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7480
StyI C'CwwG_G
Cuts at: 513 1065 3273 4449 4919 513
Size: 552 2208 1176 470 3074
Fragments arranged by size:
3074 2208 1176 552 470
SwaI ATTT'AAAT
Cuts at: 3418 3418
Size: 7480
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5507 5524 5677 5862 6307 325
Size: 1255 3927 17 153 185 445 1498
Fragments arranged by size:
3927 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2715 4269 4638
Size: 274 80 33 51 1443 738 1554 369
Cuts at: 4638 4716 5726 7438 96
Size: 78 1010 1712 138
Fragments arranged by size:
1712 1554 1443 1010 738 369 274 138
80 78 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4617 4649 7440 7472 4617
Size: 32 2791 32 4625
Fragments arranged by size:
4625 2791 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7480
XbaI T'CTAG_A
Cuts at: 3175 4816 3175
Size: 1641 5839
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3919 3919
Size: 7480
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7480
XmaI C'CCGG_G
Cuts at: 1870 4805 1870
Size: 2935 4545
XmnI GAAnn'nnTTC
Cuts at: 2418 2478 2546 3380 3710 4341 5609 2418
Size: 60 68 834 330 631 1268 4289
Fragments arranged by size:
4289 1268 834 631 330 68 60
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5288 276
Size: 53 83 186 936 3754 2468
Fragments arranged by size:
3754 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII FseI FspAI KasI NaeI
NarI NgoMIV NruI PmlI PpuMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI