Restriction map for
pFN21AB5669
(Circular) (Six-base) MAPSORT of: pFN21AB5669.seq Check: 4118 from: 1 to: 5752
pFN21AB5669 5752 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3562 278
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2595 3095 1937
Size: 658 500 4594
Fragments arranged by size:
4594 658 500
Acc65I G'GTAC_C
Cuts at: 3073 3073
Size: 5752
AclI AA'CG_TT
Cuts at: 1563 3879 4252 1563
Size: 2316 373 3063
Fragments arranged by size:
3063 2316 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2170 2918 3815 5199 5467 1632
Size: 538 748 897 1384 268 1917
Fragments arranged by size:
1917 1384 897 748 538 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5555
AflIII A'CryG_T
Cuts at: 1203 2508 4651 5628 1203
Size: 1305 2143 977 1327
Fragments arranged by size:
2143 1327 1305 977
AgeI A'CCGG_T
Cuts at: 4583 4583
Size: 5752
AhdI GACnn_n'nnGTC
Cuts at: 4481 4481
Size: 5752
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5752
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5752
AlwNI CAG_nnn'CTG
Cuts at: 2347 3495 5067 2347
Size: 1148 1572 3032
Fragments arranged by size:
3032 1572 1148
ApaLI G'TGCA_C
Cuts at: 3809 4965 3809
Size: 1156 4596
ApoI r'AATT_y
Cuts at: 1493 2129 2273 2462 2800 3061 3287 3439 5548
Size: 636 144 189 338 261 226 152 2109
Cuts at: 5548 1493
Size: 1697
Fragments arranged by size:
2109 1697 636 338 261 226 189 152
144
AseI AT'TA_AT
Cuts at: 160 1032 2232 4306 160
Size: 872 1200 2074 1606
Fragments arranged by size:
2074 1606 1200 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
AvaI C'yCGr_G
Cuts at: 1870 1943 2108 3077 1870
Size: 73 165 969 4545
Fragments arranged by size:
4545 969 165 73
BamHI G'GATC_C
Cuts at: 2011 2153 3082 3449 2011
Size: 142 929 367 4314
Fragments arranged by size:
4314 929 367 142
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3073 4528 618
Size: 325 217 627 1286 1455 1842
Fragments arranged by size:
1842 1455 1286 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3071 1391
Size: 1680 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2511 928
Size: 971 612 4169
Fragments arranged by size:
4169 971 612
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3943 3977 1903
Size: 34 2006 34 3678
Fragments arranged by size:
3678 2006 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3644 4854 3644
Size: 1210 4542
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5752
BfrBI ATG'CAT
Cuts at: 2971 2971
Size: 5752
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2039 4363 136
Size: 107 122 71 631 605 367 2324 1525
Fragments arranged by size:
2324 1525 631 605 367 122 107 71
BglII A'GATC_T
Cuts at: 5747 5747
Size: 5752
BlpI GC'TnA_GC
Cuts at: 2262 3169 2262
Size: 907 4845
Bme1580I G_kGCm'C
Cuts at: 1790 3020 3813 4969 1790
Size: 1230 793 1156 2573
Fragments arranged by size:
2573 1230 1156 793
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5752
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2097 3483 4441
Size: 416 53 83 399 24 680 1386 958
Cuts at: 4441 442
Size: 1753
Fragments arranged by size:
1753 1386 958 680 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 2155 4412 1239
Size: 842 74 2257 2579
Fragments arranged by size:
2579 2257 842 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3877 4742 5040 3877
Size: 865 298 4589
Fragments arranged by size:
4589 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4415 882
Size: 617 2916 2219
Fragments arranged by size:
2916 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3543 493
Size: 711 2339 2702
Fragments arranged by size:
2702 2339 711
BsaBI GATnn'nnATC
Cuts at: 5746 5746
Size: 5752
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3559 3941 4576 275
Size: 53 83 186 936 2026 382 635 1451
Fragments arranged by size:
2026 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2437 2467 4185 4583 4857 5004 5415 2437
Size: 30 1718 398 274 147 411 2774
Fragments arranged by size:
2774 1718 411 398 274 147 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5527
Fragments arranged by size:
5527 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2102 2307 1186
Size: 916 205 4631
Fragments arranged by size:
4631 916 205
BseYI C'CCAG_C
Cuts at: 4955 5451 4955
Size: 496 5256
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5179
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2075 3202 3963 4991 1540
Size: 275 186 74 1127 761 1028 2301
Fragments arranged by size:
2301 1127 1028 761 275 186 74
BsiHKAI G_wGCw'C
Cuts at: 3813 3898 4969 3813
Size: 85 1071 4596
Fragments arranged by size:
4596 1071 85
BsmI GAATG_Cn'
Cuts at: 2934 3263 3356 2934
Size: 329 93 5330
Fragments arranged by size:
5330 329 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3020 3071 3813 3898 4969 1391
Size: 399 1230 51 742 85 1071 2174
Fragments arranged by size:
2174 1230 1071 742 399 85 51
BspEI T'CCGG_A
Cuts at: 5415 5415
Size: 5752
BspHI T'CATG_A
Cuts at: 3641 5371 3641
Size: 1730 4022
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3107 844
Size: 2263 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3639 1394
Size: 429 1816 3507
Fragments arranged by size:
3507 1816 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4247 4421 65
Size: 1183 2999 174 1396
Fragments arranged by size:
2999 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2033 4396 4583 1700
Size: 110 223 2363 187 2869
Fragments arranged by size:
2869 2363 223 187 110
BsrGI T'GTAC_A
Cuts at: 96 2446 96
Size: 2350 3402
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5752
BssSI C'ACGA_G
Cuts at: 3812 4824 3812
Size: 1012 4740
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5752
BstBI TT'CG_AA
Cuts at: 2926 4556 2926
Size: 1630 4122
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5752
BstYI r'GATC_y
Cuts at: 1972 2011 2153 3082 3449 3834 3851 5303 5389
Size: 39 142 929 367 385 17 1452 86
Cuts at: 5389 5401 5747 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 929 385 367 346 142 86
39 17 12
Bsu36I CC'TnA_GG
Cuts at: 2616 2616
Size: 5752
BtgI C'CryG_G
Cuts at: 513 1065 2004 2043 2069 513
Size: 552 939 39 26 4196
Fragments arranged by size:
4196 939 552 39 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5013
Fragments arranged by size:
5013 683 56
BtsI GCAGTG_nn'
Cuts at: 3268 4062 4082 3268
Size: 794 20 4938
Fragments arranged by size:
4938 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5752
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5717
DraI TTT'AAA
Cuts at: 3056 3056
Size: 5752
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4759 809
Size: 507 3443 1802
Fragments arranged by size:
3443 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2072 2137 3199
Size: 54 1425 50 275 18 242 65 1062
Cuts at: 3199 4088 5717 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1062 889 275 242 65 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2072 3199 1537
Size: 275 260 1127 4090
Fragments arranged by size:
4090 1127 275 260
EarI CTCTTCn'nnn_
Cuts at: 1353 2309 3004 3682 1353
Size: 956 695 678 3423
Fragments arranged by size:
3423 956 695 678
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4339 4713 4859 387
Size: 1064 138 2750 374 146 1280
Fragments arranged by size:
2750 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2155 2170 2918 3815 4412 5199
Size: 393 449 74 15 748 897 597 787
Cuts at: 5199 5467 1239
Size: 268 1524
Fragments arranged by size:
1524 897 787 748 597 449 393 268
74 15
EcoRI G'AATT_C
Cuts at: 2273 3061 3439 2273
Size: 788 378 4586
Fragments arranged by size:
4586 788 378
FspI TGC'GCA
Cuts at: 4258 4258
Size: 5752
HaeII r_GCGC'y
Cuts at: 1678 4899 1678
Size: 3221 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4393 4425 4467 4499 1373
Size: 32 135 32 2821 32 42 32 2626
Fragments arranged by size:
2821 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2735 3096 3342 669
Size: 988 281 797 361 246 3079
Fragments arranged by size:
3079 988 797 361 281 246
HindIII A'AGCT_T
Cuts at: 748 2264 2279 748
Size: 1516 15 4221
Fragments arranged by size:
4221 1516 15
HpaI GTT'AAC
Cuts at: 3342 3342
Size: 5752
KpnI G_GTAC'C
Cuts at: 3077 3077
Size: 5752
MfeI C'AATT_G
Cuts at: 3351 3351
Size: 5752
MluI A'CGCG_T
Cuts at: 5628 5628
Size: 5752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4866 5050 1305
Size: 3561 184 2007
Fragments arranged by size:
3561 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2139 10
Size: 54 1425 343 307 3623
Fragments arranged by size:
3623 1425 343 307 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3710 4069 4228 518
Size: 648 2544 359 159 2042
Fragments arranged by size:
2544 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2071 2548 3168 3845 4993 5238 1606
Size: 465 477 620 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 620 477 465 245
NaeI GCC'GGC
Cuts at: 2035 2035
Size: 5752
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4261
Fragments arranged by size:
4261 939 552
NdeI CA'TA_TG
Cuts at: 387 2794 387
Size: 2407 3345
NgoMIV G'CCGG_C
Cuts at: 2033 2033
Size: 5752
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5752
NotI GC'GGCC_GC
Cuts at: 3199 3199
Size: 5752
NsiI A_TGCA'T
Cuts at: 2973 2973
Size: 5752
NspI r_CATG'y
Cuts at: 1132 1229 2975 3110 4655 1132
Size: 97 1746 135 1545 2229
Fragments arranged by size:
2229 1746 1545 135 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5752
PciI A'CATG_T
Cuts at: 4651 4651
Size: 5752
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5752
PmeI GTTT'AAAC
Cuts at: 3056 3056
Size: 5752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3821 3853 5334 5366 3821
Size: 32 1481 32 4207
Fragments arranged by size:
4207 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5752
PsiI TTA'TAA
Cuts at: 2334 3322 2334
Size: 988 4764
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2289 2321 2289
Size: 32 5720
PstI C_TGCA'G
Cuts at: 830 3104 830
Size: 2274 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5752
PvuII CAG'CTG
Cuts at: 2548 2548
Size: 5752
SacII CC_GC'GG
Cuts at: 2072 2072
Size: 5752
SalI G'TCGA_C
Cuts at: 1936 3094 1936
Size: 1158 4594
SbfI CC_TGCA'GG
Cuts at: 3104 3104
Size: 5752
ScaI AGT'ACT
Cuts at: 4000 5712 4000
Size: 1712 4040
SfcI C'TryA_G
Cuts at: 826 1045 3100 4235 4916 5107 826
Size: 219 2055 1135 681 191 1471
Fragments arranged by size:
2055 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5752
SmaI CCC'GGG
Cuts at: 1872 3079 1872
Size: 1207 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3856 4757 5019 820
Size: 197 926 1913 901 262 1553
Fragments arranged by size:
1913 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3543 493
Size: 3050 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5752
SphI G_CATG'C
Cuts at: 1132 2975 3110 1132
Size: 1843 135 3774
Fragments arranged by size:
3774 1843 135
SspI AAT'ATT
Cuts at: 5 52 2782 3676 5
Size: 47 2730 894 2081
Fragments arranged by size:
2730 2081 894 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5752
StyI C'CwwG_G
Cuts at: 513 1065 2004 3191 513
Size: 552 939 1187 3074
Fragments arranged by size:
3074 1187 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2931 3779 3796 3949 4134 4579 325
Size: 1255 1351 848 17 153 185 445 1498
Fragments arranged by size:
1498 1351 1255 848 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2446 3998 5710
Size: 274 80 33 51 1443 469 1552 1712
Cuts at: 5710 96
Size: 138
Fragments arranged by size:
1712 1552 1443 469 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5712 5744 5712
Size: 32 5720
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5752
XbaI T'CTAG_A
Cuts at: 3088 3088
Size: 5752
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5752
XmaI C'CCGG_G
Cuts at: 1870 3077 1870
Size: 1207 4545
XmnI GAAnn'nnTTC
Cuts at: 3881 3881
Size: 5752
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3560 276
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI
PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NarI NruI PacI PfoI PmlI PpuMI PspOMI PspXI
RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI