Restriction map for
pFN21AB5666
(Circular) (Six-base) MAPSORT of: pFN21AB5666.seq Check: 251 from: 1 to: 7606
pFN21AB5666 7606 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2348 3573 2348
Size: 1225 6381
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2361 5416 278
Size: 53 83 186 936 825 3055 2468
Fragments arranged by size:
3055 2468 936 825 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4392 4949 1937
Size: 2455 557 4594
Fragments arranged by size:
4594 2455 557
Acc65I G'GTAC_C
Cuts at: 4533 4927 4533
Size: 394 7212
AclI AA'CG_TT
Cuts at: 1563 5733 6106 1563
Size: 4170 373 3063
Fragments arranged by size:
4170 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3823 4186 4190 5669 7053 7321 1632
Size: 2191 363 4 1479 1384 268 1917
Fragments arranged by size:
2191 1917 1479 1384 363 268 4
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7409
AflIII A'CryG_T
Cuts at: 1203 3750 6505 7482 1203
Size: 2547 2755 977 1327
Fragments arranged by size:
2755 2547 1327 977
AgeI A'CCGG_T
Cuts at: 3585 6437 3585
Size: 2852 4754
AhdI GACnn_n'nnGTC
Cuts at: 3118 6335 3118
Size: 3217 4389
AleI CACnn'nnGTG
Cuts at: 1166 3608 1166
Size: 2442 5164
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2089 1184
Size: 905 6701
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2531 2563 3572 3604 2531
Size: 32 1009 32 6533
Fragments arranged by size:
6533 1009 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3502 4756 5349 6921 3502
Size: 1254 593 1572 4187
Fragments arranged by size:
4187 1572 1254 593
ApaI G_GGCC'C
Cuts at: 2917 2972 4813 2917
Size: 55 1841 5710
Fragments arranged by size:
5710 1841 55
ApaLI G'TGCA_C
Cuts at: 2074 2495 5663 6819 2074
Size: 421 3168 1156 2861
Fragments arranged by size:
3168 2861 1156 421
ApoI r'AATT_y
Cuts at: 1493 3740 4915 5141 5293 7402 1493
Size: 2247 1175 226 152 2109 1697
Fragments arranged by size:
2247 2109 1697 1175 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3990 6160 160
Size: 872 2958 2170 1606
Fragments arranged by size:
2958 2170 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7606
AvaI C'yCGr_G
Cuts at: 1870 1943 4806 4931 1870
Size: 73 2863 125 4545
Fragments arranged by size:
4545 2863 125 73
BamHI G'GATC_C
Cuts at: 2049 4521 4936 5303 2049
Size: 2472 415 367 4352
Fragments arranged by size:
4352 2472 415 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3059 3443 4533 4802 4927
Size: 325 217 627 1272 384 1090 269 125
Cuts at: 4927 6382 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1272 1090 627 384 325 269
217 125
BanII G_rGCy'C
Cuts at: 1391 2321 2478 2510 2917 2972 3285 3826 4813
Size: 930 157 32 407 55 313 541 987
Cuts at: 4813 4925 1391
Size: 112 4072
Fragments arranged by size:
4072 987 930 541 407 313 157 112
55 32
BbeI G_GCGC'C
Cuts at: 3063 3447 3063
Size: 384 7222
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2790 4838 928
Size: 971 891 2048 3696
Fragments arranged by size:
3696 2048 971 891
BbvCI CC'TCA_GC
Cuts at: 2164 2396 2164
Size: 232 7374
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2065 2099 4250 4284 4387 4421 5797
Size: 34 128 34 2151 34 103 34 1376
Cuts at: 5797 5831 1903
Size: 34 3678
Fragments arranged by size:
3678 2151 1376 128 103 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3661 5498 6708 3661
Size: 1837 1210 4559
Fragments arranged by size:
4559 1837 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7606
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2693 2933 6217
Size: 107 122 71 631 605 1021 240 3284
Cuts at: 6217 136
Size: 1525
Fragments arranged by size:
3284 1525 1021 631 605 240 122 107
71
BglII A'GATC_T
Cuts at: 7601 7601
Size: 7606
BlpI GC'TnA_GC
Cuts at: 5023 5023
Size: 7606
Bme1580I G_kGCm'C
Cuts at: 1790 2078 2409 2499 2917 2972 3765 3982 4018
Size: 288 331 90 418 55 793 217 36
Cuts at: 4018 4813 5667 6823 1790
Size: 795 854 1156 2573
Fragments arranged by size:
2573 1156 854 795 793 418 331 288
217 90 55 36
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7606
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2916 3082 5337
Size: 416 53 83 399 24 1499 166 2255
Cuts at: 5337 6295 442
Size: 958 1753
Fragments arranged by size:
2255 1753 1499 958 416 399 166 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7606
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4336 4336
Size: 7606
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2305 3112 3451 3940 4243 4778 4835 4847
Size: 1066 807 339 489 303 535 57 12
Cuts at: 4847 4865 6266 1239
Size: 18 1401 2579
Fragments arranged by size:
2579 1401 1066 807 535 489 339 303
57 18 12
Bpu10I CC'TnA_GC
Cuts at: 2164 2396 4100 4109 4538 4798 2164
Size: 232 1704 9 429 260 4972
Fragments arranged by size:
4972 1704 429 260 232 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2486 5731 6596 6894 2486
Size: 3245 865 298 3198
Fragments arranged by size:
3245 3198 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2266 3942 4324 6269 882
Size: 617 767 1676 382 1945 2219
Fragments arranged by size:
2219 1945 1676 767 617 382
BsaAI yAC'GTr
Cuts at: 493 1204 2835 2872 3253 3751 5397 493
Size: 711 1631 37 381 498 1646 2702
Fragments arranged by size:
2702 1646 1631 711 498 381 37
BsaBI GATnn'nnATC
Cuts at: 2906 7600 2906
Size: 4694 2912
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2358 3060 3423 3444
Size: 53 83 186 936 825 702 363 21
Cuts at: 3444 3506 3996 4578 5413 5795 6430 275
Size: 62 490 582 835 382 635 1451
Fragments arranged by size:
1451 936 835 825 702 635 582 490
382 363 186 83 62 53 21
BsaWI w'CCGG_w
Cuts at: 2904 3585 4566 6039 6437 6711 6858 7269 2904
Size: 681 981 1473 398 274 147 411 3241
Fragments arranged by size:
3241 1473 981 681 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3413 3443 4412 4442 1178
Size: 30 165 30 2010 30 969 30 4342
Fragments arranged by size:
4342 2010 969 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3115 3346 4325 4436 4439 1186
Size: 1929 231 979 111 3 4353
Fragments arranged by size:
4353 1929 979 231 111 3
BseYI C'CCAG_C
Cuts at: 2439 2765 6809 7305 2439
Size: 326 4044 496 2740
Fragments arranged by size:
4044 2740 496 326
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7033
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5056 5817 6845 1540
Size: 275 186 3055 761 1028 2301
Fragments arranged by size:
3055 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2078 2499 3699 4881 5667 5752 6823 2078
Size: 421 1200 1182 786 85 1071 2861
Fragments arranged by size:
2861 1200 1182 1071 786 421 85
BsmI GAATG_Cn'
Cuts at: 5117 5210 5117
Size: 93 7513
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2078 2321 2409 2478 2499 2510 2917
Size: 399 288 243 88 69 21 11 407
Cuts at: 2917 2972 3285 3699 3765 3826 3982 4018 4813
Size: 55 313 414 66 61 156 36 795
Cuts at: 4813 4881 4925 5667 5752 6823 1391
Size: 68 44 742 85 1071 2174
Fragments arranged by size:
2174 1071 795 742 414 407 399 313
288 243 156 88 85 69 68 66
61 55 44 36 21 11
BspEI T'CCGG_A
Cuts at: 2904 4566 7269 2904
Size: 1662 2703 3241
Fragments arranged by size:
3241 2703 1662
BspHI T'CATG_A
Cuts at: 3166 3621 5495 7225 3166
Size: 455 1874 1730 3547
Fragments arranged by size:
3547 1874 1730 455
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2348 3573 4168 4386 4961 844
Size: 1504 1225 595 218 575 3489
Fragments arranged by size:
3489 1504 1225 595 575 218
BsrBI CCG'CTC
Cuts at: 1394 1823 3145 4137 5493 1394
Size: 429 1322 992 1356 3507
Fragments arranged by size:
3507 1356 1322 992 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2614 2928 3859 6101 6275 65
Size: 1183 1366 314 931 2242 174 1396
Fragments arranged by size:
2242 1396 1366 1183 931 314 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2683 3056 3397 3585 6250 6437 1700
Size: 110 873 373 341 188 2665 187 2869
Fragments arranged by size:
2869 2665 873 373 341 188 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7606
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7606
BssSI C'ACGA_G
Cuts at: 5666 6678 5666
Size: 1012 6594
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3344 4284 794
Size: 2550 940 4116
Fragments arranged by size:
4116 2550 940
BstBI TT'CG_AA
Cuts at: 6410 6410
Size: 7606
BstEII G'GTnAC_C
Cuts at: 1184 3082 3199 1184
Size: 1898 117 5591
Fragments arranged by size:
5591 1898 117
BstXI CCAn_nnnn'nTGG
Cuts at: 2035 2197 2035
Size: 162 7444
BstYI r'GATC_y
Cuts at: 1972 2049 2907 4230 4521 4936 5303 5688 5705
Size: 77 858 1323 291 415 367 385 17
Cuts at: 5705 7157 7243 7255 7601 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1323 858 415 385 367 346
291 86 77 17 12
Bsu36I CC'TnA_GG
Cuts at: 3455 3455
Size: 7606
BtgI C'CryG_G
Cuts at: 513 1065 2004 2139 2941 3063 513
Size: 552 939 135 802 122 5056
Fragments arranged by size:
5056 939 802 552 135 122
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2068 487
Size: 683 56 842 6025
Fragments arranged by size:
6025 842 683 56
BtsI GCAGTG_nn'
Cuts at: 3345 4343 4722 5122 5916 5936 3345
Size: 998 379 400 794 20 5015
Fragments arranged by size:
5015 998 794 400 379 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7606
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7571
DraI TTT'AAA
Cuts at: 4910 4910
Size: 7606
DraIII CAC_nnn'GTG
Cuts at: 2413 2413
Size: 7606
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2740 6613 809
Size: 507 1424 3873 1802
Fragments arranged by size:
3873 1802 1424 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2187 2625
Size: 54 1425 50 275 18 177 180 438
Cuts at: 2625 2685 3957 4008 5053 5942 7571 8
Size: 60 1272 51 1045 889 1629 43
Fragments arranged by size:
1629 1425 1272 1045 889 438 275 180
177 60 54 51 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5053 1537
Size: 275 3241 4090
Fragments arranged by size:
4090 3241 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3842 3906 4061 4062 4196 5536 1353
Size: 2489 64 155 1 134 1340 3423
Fragments arranged by size:
3423 2489 1340 155 134 64 1
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2149 6193 6567 6713 387
Size: 1064 138 560 4044 374 146 1280
Fragments arranged by size:
4044 1280 1064 560 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2305 3112 3451 3823 3940 4186 4190
Size: 393 673 807 339 372 117 246 4
Cuts at: 4190 4243 4778 4835 4847 4865 5669 6266 7053
Size: 53 535 57 12 18 804 597 787
Cuts at: 7053 7321 1239
Size: 268 1524
Fragments arranged by size:
1524 807 804 787 673 597 535 393
372 339 268 246 117 57 53 18
12 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2399 2516 3479 3569 3689 4709 2399
Size: 117 963 90 120 1020 5296
Fragments arranged by size:
5296 1020 963 120 117 90
EcoO109I rG'GnC_Cy
Cuts at: 2299 2728 2762 2969 3499 3681 3862 4024 4810
Size: 429 34 207 530 182 181 162 786
Cuts at: 4810 2299
Size: 5095
Fragments arranged by size:
5095 786 530 429 207 182 181 162
34
EcoRI G'AATT_C
Cuts at: 3740 4915 5293 3740
Size: 1175 378 6053
Fragments arranged by size:
6053 1175 378
FspI TGC'GCA
Cuts at: 6112 6112
Size: 7606
HaeII r_GCGC'y
Cuts at: 1678 3063 3447 4043 6753 1678
Size: 1385 384 596 2710 2531
Fragments arranged by size:
2710 2531 1385 596 384
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2265 2297 3104 3136 3413
Size: 32 135 32 693 32 807 32 277
Cuts at: 3413 3445 3919 3951 4568 4600 6247 6279 6321
Size: 32 474 32 617 32 1647 32 42
Cuts at: 6321 6353 1373
Size: 32 2626
Fragments arranged by size:
2626 1647 807 693 617 474 277 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4950 5196 669
Size: 988 281 3012 246 3079
Fragments arranged by size:
3079 3012 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4661 748
Size: 3913 3693
HpaI GTT'AAC
Cuts at: 5196 5196
Size: 7606
KasI G'GCGC_C
Cuts at: 3059 3443 3059
Size: 384 7222
KpnI G_GTAC'C
Cuts at: 4537 4931 4537
Size: 394 7212
MfeI C'AATT_G
Cuts at: 5205 5205
Size: 7606
MluI A'CGCG_T
Cuts at: 7482 7482
Size: 7606
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2769 3619 4314 4725 6720 6904 1305
Size: 1464 850 695 411 1995 184 2007
Fragments arranged by size:
2007 1995 1464 850 695 411 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2627 4010 10
Size: 54 1425 343 177 618 1383 3606
Fragments arranged by size:
3606 1425 1383 618 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3608 3662 5564 5923 6082 518
Size: 648 2442 54 1902 359 159 2042
Fragments arranged by size:
2442 2042 1902 648 359 159 54
MspA1I CmG'CkG
Cuts at: 1606 3367 3630 3940 4756 5022 5699 6847 7092
Size: 1761 263 310 816 266 677 1148 245
Cuts at: 7092 1606
Size: 2120
Fragments arranged by size:
2120 1761 1148 816 677 310 266 263
245
NaeI GCC'GGC
Cuts at: 2685 3058 3399 2685
Size: 373 341 6892
Fragments arranged by size:
6892 373 341
NarI GG'CG_CC
Cuts at: 3060 3444 3060
Size: 384 7222
NcoI C'CATG_G
Cuts at: 513 1065 2004 3063 513
Size: 552 939 1059 5056
Fragments arranged by size:
5056 1059 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7606
NgoMIV G'CCGG_C
Cuts at: 2683 3056 3397 2683
Size: 373 341 6892
Fragments arranged by size:
6892 373 341
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7606
NotI GC'GGCC_GC
Cuts at: 5053 5053
Size: 7606
NspI r_CATG'y
Cuts at: 1132 1229 2752 4084 4964 6509 1132
Size: 97 1523 1332 880 1545 2229
Fragments arranged by size:
2229 1545 1523 1332 880 97
PasI CC'CwG_GG
Cuts at: 1348 2035 4086 4411 1348
Size: 687 2051 325 4543
Fragments arranged by size:
4543 2051 687 325
PciI A'CATG_T
Cuts at: 6505 6505
Size: 7606
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3210 4221 1108
Size: 2102 1011 4493
Fragments arranged by size:
4493 2102 1011
PmeI GTTT'AAAC
Cuts at: 4910 4910
Size: 7606
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5675 5707 7188 7220 5675
Size: 32 1481 32 6061
Fragments arranged by size:
6061 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2728 2762 3681 3862 4024 2728
Size: 34 919 181 162 6310
Fragments arranged by size:
6310 919 181 162 34
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7606
PsiI TTA'TAA
Cuts at: 5176 5176
Size: 7606
PspOMI G'GGCC_C
Cuts at: 2913 2968 4809 2913
Size: 55 1841 5710
Fragments arranged by size:
5710 1841 55
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 7574
PstI C_TGCA'G
Cuts at: 830 2650 3570 3642 3945 4508 4752 4958 830
Size: 1820 920 72 303 563 244 206 3478
Fragments arranged by size:
3478 1820 920 563 303 244 206 72
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7606
PvuII CAG'CTG
Cuts at: 3940 4756 3940
Size: 816 6790
SalI G'TCGA_C
Cuts at: 1936 4948 1936
Size: 3012 4594
SanDI GG'GwC_CC
Cuts at: 3681 3681
Size: 7606
SbfI CC_TGCA'GG
Cuts at: 3570 4958 3570
Size: 1388 6218
ScaI AGT'ACT
Cuts at: 5854 7566 5854
Size: 1712 5894
SexAI A'CCwGG_T
Cuts at: 2487 3195 2487
Size: 708 6898
SfcI C'TryA_G
Cuts at: 826 1045 2646 3433 3566 3638 3941 4504 4748
Size: 219 1601 787 133 72 303 563 244
Cuts at: 4748 4954 6089 6770 6961 826
Size: 206 1135 681 191 1471
Fragments arranged by size:
1601 1471 1135 787 681 563 303 244
219 206 191 133 72
SfiI GGCCn_nnn'nGGCC
Cuts at: 2693 2693
Size: 7606
SfoI GGC'GCC
Cuts at: 3061 3445 3061
Size: 384 7222
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7606
SgrAI Cr'CCGG_yG
Cuts at: 1700 3056 1700
Size: 1356 6250
SmaI CCC'GGG
Cuts at: 1872 4808 4933 1872
Size: 2936 125 4545
Fragments arranged by size:
4545 2936 125
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2501 5710 6611 6873 820
Size: 197 926 558 3209 901 262 1553
Fragments arranged by size:
3209 1553 926 901 558 262 197
SnaBI TAC'GTA
Cuts at: 493 5397 493
Size: 4904 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7606
SphI G_CATG'C
Cuts at: 1132 4964 1132
Size: 3832 3774
SspI AAT'ATT
Cuts at: 5 52 5530 5
Size: 47 5478 2081
Fragments arranged by size:
5478 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7606
StyI C'CwwG_G
Cuts at: 513 1065 2004 2010 2196 2724 3063 5045 513
Size: 552 939 6 186 528 339 1982 3074
Fragments arranged by size:
3074 1982 939 552 528 339 186 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3254 4843 5633 5650 5803 5988 6433
Size: 1255 1674 1589 790 17 153 185 445
Cuts at: 6433 325
Size: 1498
Fragments arranged by size:
1674 1589 1498 1255 790 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2100 2595 4077
Size: 274 80 33 51 1443 123 495 1482
Cuts at: 4077 4254 5852 7564 96
Size: 177 1598 1712 138
Fragments arranged by size:
1712 1598 1482 1443 495 274 177 138
123 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2761 2793 3353 3385 4412 4444 7566 7598 2761
Size: 32 560 32 1027 32 3122 32 2769
Fragments arranged by size:
3122 2769 1027 560 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4640 1316
Size: 3324 4282
XbaI T'CTAG_A
Cuts at: 4942 4942
Size: 7606
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2485 2839 2485
Size: 354 7252
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7606
XmaI C'CCGG_G
Cuts at: 1870 4806 4931 1870
Size: 2936 125 4545
Fragments arranged by size:
4545 2936 125
XmnI GAAnn'nnTTC
Cuts at: 2663 5735 2663
Size: 3072 4534
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2359 5414 276
Size: 53 83 186 936 825 3055 2468
Fragments arranged by size:
3055 2468 936 825 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI
SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I
EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI
PfoI PmlI PspXI RsrII SacI SacII SapI SrfI
SwaI