Restriction map for
pFN21AB5664
(Circular) (Six-base) MAPSORT of: pFN21AB5664.seq Check: 6318 from: 1 to: 5839
pFN21AB5664 5839 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2390 2390
Size: 5839
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3649 278
Size: 53 83 186 936 2113 2468
Fragments arranged by size:
2468 2113 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3182 1937
Size: 1245 4594
Acc65I G'GTAC_C
Cuts at: 3160 3160
Size: 5839
AclI AA'CG_TT
Cuts at: 1563 3138 3966 4339 1563
Size: 1575 828 373 3063
Fragments arranged by size:
3063 1575 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2120 3101 3902 5286 5554 1632
Size: 488 981 801 1384 268 1917
Fragments arranged by size:
1917 1384 981 801 488 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5642
AflIII A'CryG_T
Cuts at: 1203 4738 5715 1203
Size: 3535 977 1327
Fragments arranged by size:
3535 1327 977
AgeI A'CCGG_T
Cuts at: 4670 4670
Size: 5839
AhdI GACnn_n'nnGTC
Cuts at: 4568 4568
Size: 5839
AleI CACnn'nnGTG
Cuts at: 1166 2132 1166
Size: 966 4873
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2933 1184
Size: 1749 4090
AlwNI CAG_nnn'CTG
Cuts at: 3582 5154 3582
Size: 1572 4267
ApaI G_GGCC'C
Cuts at: 2974 2974
Size: 5839
ApaLI G'TGCA_C
Cuts at: 3896 5052 3896
Size: 1156 4683
ApoI r'AATT_y
Cuts at: 1493 2210 3148 3374 3526 5635 1493
Size: 717 938 226 152 2109 1697
Fragments arranged by size:
2109 1697 938 717 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4393 160
Size: 872 3361 1606
Fragments arranged by size:
3361 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
AvaI C'yCGr_G
Cuts at: 1870 1943 3164 1870
Size: 73 1221 4545
Fragments arranged by size:
4545 1221 73
BamHI G'GATC_C
Cuts at: 3169 3536 3169
Size: 367 5472
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2457 2510 3160 4615 618
Size: 325 217 627 670 53 650 1455 1842
Fragments arranged by size:
1842 1455 670 650 627 325 217 53
BanII G_rGCy'C
Cuts at: 1391 2974 3158 1391
Size: 1583 184 4072
Fragments arranged by size:
4072 1583 184
BbeI G_GCGC'C
Cuts at: 2514 2514
Size: 5839
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2315 2458 3069 928
Size: 971 416 143 611 3698
Fragments arranged by size:
3698 971 611 416 143
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2143 2177 2341 2375 4030 4064 1903
Size: 34 206 34 164 34 1655 34 3678
Fragments arranged by size:
3678 1655 206 164 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3731 4941 3731
Size: 1210 4629
BclI T'GATC_A
Cuts at: 1548 2424 1548
Size: 876 4963
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2372 4450 136
Size: 107 122 71 631 605 700 2078 1525
Fragments arranged by size:
2078 1525 700 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5834 5834
Size: 5839
BlpI GC'TnA_GC
Cuts at: 3256 3256
Size: 5839
Bme1580I G_kGCm'C
Cuts at: 1790 2828 2974 3900 5056 1790
Size: 1038 146 926 1156 2573
Fragments arranged by size:
2573 1156 1038 926 146
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5839
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3570 4528 442
Size: 416 53 83 399 24 2153 958 1753
Fragments arranged by size:
2153 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2015 4499 1239
Size: 776 2484 2579
Fragments arranged by size:
2579 2484 776
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3964 4829 5127 3964
Size: 865 298 4676
Fragments arranged by size:
4676 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2045 4502 882
Size: 617 546 2457 2219
Fragments arranged by size:
2457 2219 617 546
BsaAI yAC'GTr
Cuts at: 493 1204 2077 3630 493
Size: 711 873 1553 2702
Fragments arranged by size:
2702 1553 873 711
BsaBI GATnn'nnATC
Cuts at: 2192 5833 2192
Size: 3641 2198
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2375 2445 2511 3646
Size: 53 83 186 936 842 70 66 1135
Cuts at: 3646 4028 4663 275
Size: 382 635 1451
Fragments arranged by size:
1451 1135 936 842 635 382 186 83
70 66 53
BsaWI w'CCGG_w
Cuts at: 4272 4670 4944 5091 5502 4272
Size: 398 274 147 411 4609
Fragments arranged by size:
4609 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5614
Fragments arranged by size:
5614 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5839
BseYI C'CCAG_C
Cuts at: 2506 5042 5538 2506
Size: 2536 496 2807
Fragments arranged by size:
2807 2536 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2525 2837 1163
Size: 573 789 312 4165
Fragments arranged by size:
4165 789 573 312
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3289 4050 5078 1540
Size: 275 186 1288 761 1028 2301
Fragments arranged by size:
2301 1288 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2139 2871 3900 3985 5056 2139
Size: 732 1029 85 1071 2922
Fragments arranged by size:
2922 1071 1029 732 85
BsmI GAATG_Cn'
Cuts at: 3350 3443 3350
Size: 93 5746
BsmBI CGTCTCn'nnnn_
Cuts at: 2033 2033
Size: 5839
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2139 2828 2871 2974 3158 3900 3985
Size: 399 349 689 43 103 184 742 85
Cuts at: 3985 5056 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 689 399 349 184 103
85 43
BspEI T'CCGG_A
Cuts at: 5502 5502
Size: 5839
BspHI T'CATG_A
Cuts at: 3728 5458 3728
Size: 1730 4109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2268 2390 3075 3194 844
Size: 1424 122 685 119 3489
Fragments arranged by size:
3489 1424 685 122 119
BsrBI CCG'CTC
Cuts at: 1394 1823 2410 2713 3726 1394
Size: 429 587 303 1013 3507
Fragments arranged by size:
3507 1013 587 429 303
BsrDI GCAATG_nn'
Cuts at: 65 1248 2493 2592 4334 4508 65
Size: 1183 1245 99 1742 174 1396
Fragments arranged by size:
1742 1396 1245 1183 174 99
BsrFI r'CCGG_y
Cuts at: 1700 1810 2035 2267 4483 4670 1700
Size: 110 225 232 2216 187 2869
Fragments arranged by size:
2869 2216 232 225 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5839
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5839
BssSI C'ACGA_G
Cuts at: 3899 4911 3899
Size: 1012 4827
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5839
BstBI TT'CG_AA
Cuts at: 4643 4643
Size: 5839
BstEII G'GTnAC_C
Cuts at: 1184 2629 1184
Size: 1445 4394
BstYI r'GATC_y
Cuts at: 1972 3126 3169 3536 3921 3938 5390 5476 5488
Size: 1154 43 367 385 17 1452 86 12
Cuts at: 5488 5834 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1154 385 367 346 86 43
17 12
Bsu36I CC'TnA_GG
Cuts at: 2342 2342
Size: 5839
BtgI C'CryG_G
Cuts at: 513 1065 2004 2328 2718 2736 513
Size: 552 939 324 390 18 3616
Fragments arranged by size:
3616 939 552 390 324 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2257 2383 2746 487
Size: 683 56 1031 126 363 3580
Fragments arranged by size:
3580 1031 683 363 126 56
BtsI GCAGTG_nn'
Cuts at: 2723 3355 4149 4169 2723
Size: 632 794 20 4393
Fragments arranged by size:
4393 794 632 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5839
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5804
DraI TTT'AAA
Cuts at: 3143 3143
Size: 5839
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4846 809
Size: 507 3530 1802
Fragments arranged by size:
3530 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2265 2394 2619
Size: 54 1425 50 275 18 435 129 225
Cuts at: 2619 2691 2904 3286 4175 5804 8
Size: 72 213 382 889 1629 43
Fragments arranged by size:
1629 1425 889 435 382 275 225 213
129 72 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3286 1537
Size: 275 1474 4090
Fragments arranged by size:
4090 1474 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2144 2786 2795 2840 3769 1353
Size: 791 642 9 45 929 3423
Fragments arranged by size:
3423 929 791 642 45 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2866 4426 4800 4946 387
Size: 1064 138 1277 1560 374 146 1280
Fragments arranged by size:
1560 1280 1277 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2015 2120 3101 3902 4499 5286 5554
Size: 393 383 105 981 801 597 787 268
Cuts at: 5554 1239
Size: 1524
Fragments arranged by size:
1524 981 801 787 597 393 383 268
105
EcoNI CCTnn'n_nnAGG
Cuts at: 2345 2837 2345
Size: 492 5347
EcoO109I rG'GnC_Cy
Cuts at: 2970 2970
Size: 5839
EcoRI G'AATT_C
Cuts at: 3148 3526 3148
Size: 378 5461
FspI TGC'GCA
Cuts at: 4345 4345
Size: 5839
HaeII r_GCGC'y
Cuts at: 1678 2514 4986 1678
Size: 836 2472 2531
Fragments arranged by size:
2531 2472 836
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4480 4512 4554 4586 1373
Size: 32 135 32 2908 32 42 32 2626
Fragments arranged by size:
2908 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2287 3183 3429 669
Size: 988 281 349 896 246 3079
Fragments arranged by size:
3079 988 896 349 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5839
HpaI GTT'AAC
Cuts at: 3429 3429
Size: 5839
KasI G'GCGC_C
Cuts at: 2510 2510
Size: 5839
KpnI G_GTAC'C
Cuts at: 3164 3164
Size: 5839
MfeI C'AATT_G
Cuts at: 3438 3438
Size: 5839
MluI A'CGCG_T
Cuts at: 5715 5715
Size: 5839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4953 5137 1305
Size: 3648 184 2007
Fragments arranged by size:
3648 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2396 2693 2906 10
Size: 54 1425 343 564 297 213 2943
Fragments arranged by size:
2943 1425 564 343 297 213 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2132 2294 2627 2801 3797 4156 4315
Size: 648 966 162 333 174 996 359 159
Cuts at: 4315 518
Size: 2042
Fragments arranged by size:
2042 996 966 648 359 333 174 162
159
MspA1I CmG'CkG
Cuts at: 1606 3255 3932 5080 5325 1606
Size: 1649 677 1148 245 2120
Fragments arranged by size:
2120 1649 1148 677 245
NaeI GCC'GGC
Cuts at: 2269 2269
Size: 5839
NarI GG'CG_CC
Cuts at: 2511 2511
Size: 5839
NcoI C'CATG_G
Cuts at: 513 1065 2004 2718 513
Size: 552 939 714 3634
Fragments arranged by size:
3634 939 714 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5839
NgoMIV G'CCGG_C
Cuts at: 2267 2267
Size: 5839
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5839
NotI GC'GGCC_GC
Cuts at: 3286 3286
Size: 5839
NspI r_CATG'y
Cuts at: 1132 1229 2227 3197 4742 1132
Size: 97 998 970 1545 2229
Fragments arranged by size:
2229 1545 998 970 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5839
PciI A'CATG_T
Cuts at: 4738 4738
Size: 5839
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2077 2334 1108
Size: 969 257 4613
Fragments arranged by size:
4613 969 257
PmeI GTTT'AAAC
Cuts at: 3143 3143
Size: 5839
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3908 3940 5421 5453 3908
Size: 32 1481 32 4294
Fragments arranged by size:
4294 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5839
PsiI TTA'TAA
Cuts at: 3409 3409
Size: 5839
PspOMI G'GGCC_C
Cuts at: 2970 2970
Size: 5839
PstI C_TGCA'G
Cuts at: 830 2156 2387 2813 3191 830
Size: 1326 231 426 378 3478
Fragments arranged by size:
3478 1326 426 378 231
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5839
RsrII CG'GwC_CG
Cuts at: 2733 2733
Size: 5839
SalI G'TCGA_C
Cuts at: 1936 3181 1936
Size: 1245 4594
SapI GCTCTTCn'nnn_
Cuts at: 2144 2144
Size: 5839
SbfI CC_TGCA'GG
Cuts at: 2813 3191 2813
Size: 378 5461
ScaI AGT'ACT
Cuts at: 4087 5799 4087
Size: 1712 4127
SfcI C'TryA_G
Cuts at: 826 1045 2152 2383 2809 3187 4322 5003 5194
Size: 219 1107 231 426 378 1135 681 191
Cuts at: 5194 826
Size: 1471
Fragments arranged by size:
1471 1135 1107 681 426 378 231 219
191
SfoI GGC'GCC
Cuts at: 2512 2512
Size: 5839
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5839
SmaI CCC'GGG
Cuts at: 1872 3166 1872
Size: 1294 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3943 4844 5106 820
Size: 197 926 2000 901 262 1553
Fragments arranged by size:
2000 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3630 493
Size: 3137 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5839
SphI G_CATG'C
Cuts at: 1132 3197 1132
Size: 2065 3774
SspI AAT'ATT
Cuts at: 5 52 3763 5
Size: 47 3711 2081
Fragments arranged by size:
3711 2081 47
StuI AGG'CCT
Cuts at: 1517 2108 2437 1517
Size: 591 329 4919
Fragments arranged by size:
4919 591 329
StyI C'CwwG_G
Cuts at: 513 1065 2004 2368 2718 3278 513
Size: 552 939 364 350 560 3074
Fragments arranged by size:
3074 939 560 552 364 350
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2998 3866 3883 4036 4221 4666 325
Size: 1255 1418 868 17 153 185 445 1498
Fragments arranged by size:
1498 1418 1255 868 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2226 2589 4085
Size: 274 80 33 51 1443 249 363 1496
Cuts at: 4085 5797 96
Size: 1712 138
Fragments arranged by size:
1712 1496 1443 363 274 249 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2769 2801 5799 5831 2769
Size: 32 2998 32 2777
Fragments arranged by size:
2998 2777 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5839
XbaI T'CTAG_A
Cuts at: 3175 3175
Size: 5839
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5839
XmaI C'CCGG_G
Cuts at: 1870 3164 1870
Size: 1294 4545
XmnI GAAnn'nnTTC
Cuts at: 2312 3968 2312
Size: 1656 4183
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3647 276
Size: 53 83 186 936 2113 2468
Fragments arranged by size:
2468 2113 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI RsrII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI PvuII SacI SacII SanDI SexAI SfiI
SrfI SwaI XcmI