Restriction map for
pFN21AB5662
(Circular) (Six-base) MAPSORT of: pFN21AB5662.seq Check: 7520 from: 1 to: 5821
pFN21AB5662 5821 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3631 278
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3164 1937
Size: 1227 4594
Acc65I G'GTAC_C
Cuts at: 3142 3142
Size: 5821
AclI AA'CG_TT
Cuts at: 1563 3948 4321 1563
Size: 2385 373 3063
Fragments arranged by size:
3063 2385 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2642 3048 3884 5268 5536 1632
Size: 1010 406 836 1384 268 1917
Fragments arranged by size:
1917 1384 1010 836 406 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5624
AflIII A'CryG_T
Cuts at: 1203 4720 5697 1203
Size: 3517 977 1327
Fragments arranged by size:
3517 1327 977
AgeI A'CCGG_T
Cuts at: 4652 4652
Size: 5821
AhdI GACnn_n'nnGTC
Cuts at: 4550 4550
Size: 5821
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5821
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5821
AlwNI CAG_nnn'CTG
Cuts at: 3564 5136 3564
Size: 1572 4249
ApaI G_GGCC'C
Cuts at: 2268 2268
Size: 5821
ApaLI G'TGCA_C
Cuts at: 3878 5034 3878
Size: 1156 4665
ApoI r'AATT_y
Cuts at: 1493 2889 3130 3356 3508 5617 1493
Size: 1396 241 226 152 2109 1697
Fragments arranged by size:
2109 1697 1396 241 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4375 160
Size: 872 3343 1606
Fragments arranged by size:
3343 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
AvaI C'yCGr_G
Cuts at: 1870 1943 2082 2322 2993 3035 3146 1870
Size: 73 139 240 671 42 111 4545
Fragments arranged by size:
4545 671 240 139 111 73 42
BamHI G'GATC_C
Cuts at: 3151 3518 3151
Size: 367 5454
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 2940 3142 4597 618
Size: 325 217 627 252 901 202 1455 1842
Fragments arranged by size:
1842 1455 901 627 325 252 217 202
BanII G_rGCy'C
Cuts at: 1391 2268 2756 2973 3140 1391
Size: 877 488 217 167 4072
Fragments arranged by size:
4072 877 488 217 167
BbeI G_GCGC'C
Cuts at: 2043 2043
Size: 5821
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2470 2632 2691 928
Size: 971 571 162 59 4058
Fragments arranged by size:
4058 971 571 162 59
BbvCI CC'TCA_GC
Cuts at: 2183 2183
Size: 5821
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4012 4046 1903
Size: 34 50 34 1991 34 3678
Fragments arranged by size:
3678 1991 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3713 4923 3713
Size: 1210 4611
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5821
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2193 4432 136
Size: 107 122 71 631 605 521 2239 1525
Fragments arranged by size:
2239 1525 631 605 521 122 107 71
BglII A'GATC_T
Cuts at: 5816 5816
Size: 5821
BlpI GC'TnA_GC
Cuts at: 3238 3238
Size: 5821
Bme1580I G_kGCm'C
Cuts at: 1790 2147 2268 2878 3882 5038 1790
Size: 357 121 610 1004 1156 2573
Fragments arranged by size:
2573 1156 1004 610 357 121
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5821
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2784 2865 3552
Size: 416 53 83 399 24 1367 81 687
Cuts at: 3552 4510 442
Size: 958 1753
Fragments arranged by size:
1753 1367 958 687 416 399 83 81
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2149 4481 1239
Size: 910 2332 2579
Fragments arranged by size:
2579 2332 910
Bpu10I CC'TnA_GC
Cuts at: 2183 2627 2916 2183
Size: 444 289 5088
Fragments arranged by size:
5088 444 289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2980 3946 4811 5109 2980
Size: 966 865 298 3692
Fragments arranged by size:
3692 966 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4484 882
Size: 617 2985 2219
Fragments arranged by size:
2985 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2749 3612 493
Size: 711 1545 863 2702
Fragments arranged by size:
2702 1545 863 711
BsaBI GATnn'nnATC
Cuts at: 5815 5815
Size: 5821
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2040 2088 2112 3628
Size: 53 83 186 936 507 48 24 1516
Cuts at: 3628 4010 4645 275
Size: 382 635 1451
Fragments arranged by size:
1516 1451 936 635 507 382 186 83
53 48 24
BsaWI w'CCGG_w
Cuts at: 2017 2786 4254 4652 4926 5073 5484 2017
Size: 769 1468 398 274 147 411 2354
Fragments arranged by size:
2354 1468 769 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2492 2522 1178
Size: 30 165 30 1089 30 4477
Fragments arranged by size:
4477 1089 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2666 1186
Size: 1480 4341
BseYI C'CCAG_C
Cuts at: 2074 2267 2611 2661 2740 5024 5520 2074
Size: 193 344 50 79 2284 496 2375
Fragments arranged by size:
2375 2284 496 344 193 79 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2990 1163
Size: 573 1254 3994
Fragments arranged by size:
3994 1254 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3271 4032 5060 1540
Size: 275 186 1270 761 1028 2301
Fragments arranged by size:
2301 1270 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2165 2922 3882 3967 5038 2165
Size: 757 960 85 1071 2948
Fragments arranged by size:
2948 1071 960 757 85
BsmI GAATG_Cn'
Cuts at: 2910 3332 3425 2910
Size: 422 93 5306
Fragments arranged by size:
5306 422 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2956 2956
Size: 5821
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2147 2165 2268 2756 2878 2922 2973
Size: 399 357 18 103 488 122 44 51
Cuts at: 2973 3140 3882 3967 5038 1391
Size: 167 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 488 399 357 167 122
103 85 51 44 18
BspEI T'CCGG_A
Cuts at: 2786 5484 2786
Size: 2698 3123
BspHI T'CATG_A
Cuts at: 3710 5440 3710
Size: 1730 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2295 3176 844
Size: 1451 881 3489
Fragments arranged by size:
3489 1451 881
BsrBI CCG'CTC
Cuts at: 1394 1823 2037 2228 3708 1394
Size: 429 214 191 1480 3507
Fragments arranged by size:
3507 1480 429 214 191
BsrDI GCAATG_nn'
Cuts at: 65 1248 4316 4490 65
Size: 1183 3068 174 1396
Fragments arranged by size:
3068 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2237 4465 4652 1700
Size: 110 427 2228 187 2869
Fragments arranged by size:
2869 2228 427 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5821
BssHII G'CGCG_C
Cuts at: 1925 2256 1925
Size: 331 5490
BssSI C'ACGA_G
Cuts at: 3881 4893 3881
Size: 1012 4809
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5821
BstBI TT'CG_AA
Cuts at: 4625 4625
Size: 5821
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5821
BstXI CCAn_nnnn'nTGG
Cuts at: 2611 2611
Size: 5821
BstYI r'GATC_y
Cuts at: 1972 2760 2997 3151 3518 3903 3920 5372 5458
Size: 788 237 154 367 385 17 1452 86
Cuts at: 5458 5470 5816 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 788 385 367 346 237 154
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2053 2646 513
Size: 552 988 593 3688
Fragments arranged by size:
3688 988 593 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2260 2403 487
Size: 683 56 1034 143 3905
Fragments arranged by size:
3905 1034 683 143 56
BtsI GCAGTG_nn'
Cuts at: 2587 2985 3337 4131 4151 2587
Size: 398 352 794 20 4257
Fragments arranged by size:
4257 794 398 352 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5821
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5786
DraI TTT'AAA
Cuts at: 3125 3125
Size: 5821
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2676 4828 809
Size: 507 1360 2152 1802
Fragments arranged by size:
2152 1802 1360 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2094 2136 3268
Size: 54 1425 50 275 18 264 42 1132
Cuts at: 3268 4157 5786 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1132 889 275 264 54 50
43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 3268 1537
Size: 275 1456 4090
Fragments arranged by size:
4090 1456 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2075 2522 2535 2564 3751 1353
Size: 722 447 13 29 1187 3423
Fragments arranged by size:
3423 1187 722 447 29 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2256 2566 4408 4782 4928 387
Size: 1064 138 667 310 1842 374 146 1280
Fragments arranged by size:
1842 1280 1064 667 374 310 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2149 2642 3048 3884 4481 5268 5536
Size: 393 517 493 406 836 597 787 268
Cuts at: 5536 1239
Size: 1524
Fragments arranged by size:
1524 836 787 597 517 493 406 393
268
EcoO109I rG'GnC_Cy
Cuts at: 2264 2617 2778 2264
Size: 353 161 5307
Fragments arranged by size:
5307 353 161
EcoRI G'AATT_C
Cuts at: 3130 3508 3130
Size: 378 5443
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2471 2656 2471
Size: 185 5636
FspI TGC'GCA
Cuts at: 4327 4327
Size: 5821
HaeII r_GCGC'y
Cuts at: 1678 2043 2330 4968 1678
Size: 365 287 2638 2531
Fragments arranged by size:
2638 2531 365 287
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2303 2335 2492 2524 2705
Size: 32 135 32 731 32 157 32 181
Cuts at: 2705 2737 4462 4494 4536 4568 1373
Size: 32 1725 32 42 32 2626
Fragments arranged by size:
2626 1725 731 181 157 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3165 3411 669
Size: 988 281 1227 246 3079
Fragments arranged by size:
3079 1227 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2624 748
Size: 1876 3945
HpaI GTT'AAC
Cuts at: 3411 3411
Size: 5821
KasI G'GCGC_C
Cuts at: 2039 2039
Size: 5821
KpnI G_GTAC'C
Cuts at: 3146 3146
Size: 5821
MfeI C'AATT_G
Cuts at: 3420 3420
Size: 5821
MluI A'CGCG_T
Cuts at: 5697 5697
Size: 5821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2821 4935 5119 1305
Size: 1516 2114 184 2007
Fragments arranged by size:
2114 2007 1516 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3999
Fragments arranged by size:
3999 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2511 3779 4138 4297 518
Size: 648 1345 1268 359 159 2042
Fragments arranged by size:
2042 1345 1268 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2055 2413 3237 3914 5062 5307 1606
Size: 449 358 824 677 1148 245 2120
Fragments arranged by size:
2120 1148 824 677 449 358 245
NaeI GCC'GGC
Cuts at: 2239 2239
Size: 5821
NarI GG'CG_CC
Cuts at: 2040 2040
Size: 5821
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5269
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5821
NgoMIV G'CCGG_C
Cuts at: 2237 2237
Size: 5821
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5821
NotI GC'GGCC_GC
Cuts at: 3268 3268
Size: 5821
NspI r_CATG'y
Cuts at: 1132 1229 2318 2506 3179 4724 1132
Size: 97 1089 188 673 1545 2229
Fragments arranged by size:
2229 1545 1089 673 188 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5821
PciI A'CATG_T
Cuts at: 4720 4720
Size: 5821
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5821
PfoI T'CCnGG_A
Cuts at: 2249 2249
Size: 5821
PmeI GTTT'AAAC
Cuts at: 3125 3125
Size: 5821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3890 3922 5403 5435 3890
Size: 32 1481 32 4276
Fragments arranged by size:
4276 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2617 2778 2617
Size: 161 5660
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5821
PsiI TTA'TAA
Cuts at: 3391 3391
Size: 5821
PspOMI G'GGCC_C
Cuts at: 2264 2264
Size: 5821
PstI C_TGCA'G
Cuts at: 830 2207 3173 830
Size: 1377 966 3478
Fragments arranged by size:
3478 1377 966
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5821
PvuII CAG'CTG
Cuts at: 2413 2413
Size: 5821
RsrII CG'GwC_CG
Cuts at: 2015 2015
Size: 5821
SacII CC_GC'GG
Cuts at: 2056 2056
Size: 5821
SalI G'TCGA_C
Cuts at: 1936 3163 1936
Size: 1227 4594
SapI GCTCTTCn'nnn_
Cuts at: 2075 2564 2075
Size: 489 5332
SbfI CC_TGCA'GG
Cuts at: 3173 3173
Size: 5821
ScaI AGT'ACT
Cuts at: 4069 5781 4069
Size: 1712 4109
SfcI C'TryA_G
Cuts at: 826 1045 2203 3169 4304 4985 5176 826
Size: 219 1158 966 1135 681 191 1471
Fragments arranged by size:
1471 1158 1135 966 681 219 191
SfoI GGC'GCC
Cuts at: 2041 2041
Size: 5821
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5821
SmaI CCC'GGG
Cuts at: 1872 2324 3037 3148 1872
Size: 452 713 111 4545
Fragments arranged by size:
4545 713 452 111
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2959 3925 4826 5088 820
Size: 197 926 1016 966 901 262 1553
Fragments arranged by size:
1553 1016 966 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3612 493
Size: 3119 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5821
SphI G_CATG'C
Cuts at: 1132 3179 1132
Size: 2047 3774
SspI AAT'ATT
Cuts at: 5 52 3745 5
Size: 47 3693 2081
Fragments arranged by size:
3693 2081 47
StuI AGG'CCT
Cuts at: 1517 2401 2471 1517
Size: 884 70 4867
Fragments arranged by size:
4867 884 70
StyI C'CwwG_G
Cuts at: 513 1065 2455 2466 3260 513
Size: 552 1390 11 794 3074
Fragments arranged by size:
3074 1390 794 552 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2003 3848 3865 4018 4203 4648 325
Size: 1255 423 1845 17 153 185 445 1498
Fragments arranged by size:
1845 1498 1255 445 423 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4067 5779 96
Size: 274 80 33 51 1443 2090 1712 138
Fragments arranged by size:
2090 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5781 5813 5781
Size: 32 5789
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5821
XbaI T'CTAG_A
Cuts at: 2680 3157 2680
Size: 477 5344
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2104 2104
Size: 5821
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5821
XmaI C'CCGG_G
Cuts at: 1870 2322 3035 3146 1870
Size: 452 713 111 4545
Fragments arranged by size:
4545 713 452 111
XmnI GAAnn'nnTTC
Cuts at: 3950 3950
Size: 5821
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3629 276
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI
FspAI NruI NsiI PacI PmlI PspXI PsrI SacI
SanDI SexAI SfiI SrfI SwaI