Restriction map for
pFN21AB5657
(Circular) (Six-base) MAPSORT of: pFN21AB5657.seq Check: 8940 from: 1 to: 8137
pFN21AB5657 8137 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5947 278
Size: 53 83 186 936 4411 2468
Fragments arranged by size:
4411 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5480 1937
Size: 3543 4594
Acc65I G'GTAC_C
Cuts at: 3758 3948 4698 5458 3758
Size: 190 750 760 6437
Fragments arranged by size:
6437 760 750 190
AclI AA'CG_TT
Cuts at: 1563 6264 6637 1563
Size: 4701 373 3063
Fragments arranged by size:
4701 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4023 4287 6200 7584 7852 1632
Size: 2391 264 1913 1384 268 1917
Fragments arranged by size:
2391 1917 1913 1384 268 264
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7940
AflIII A'CryG_T
Cuts at: 1203 7036 8013 1203
Size: 5833 977 1327
Fragments arranged by size:
5833 1327 977
AgeI A'CCGG_T
Cuts at: 6968 6968
Size: 8137
AhdI GACnn_n'nnGTC
Cuts at: 6866 6866
Size: 8137
AleI CACnn'nnGTG
Cuts at: 1166 3081 1166
Size: 1915 6222
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8137
AlwNI CAG_nnn'CTG
Cuts at: 2824 3728 5880 7452 2824
Size: 904 2152 1572 3509
Fragments arranged by size:
3509 2152 1572 904
ApaLI G'TGCA_C
Cuts at: 6194 7350 6194
Size: 1156 6981
ApoI r'AATT_y
Cuts at: 1493 3352 3591 3641 3688 3805 4183 4236 4647
Size: 1859 239 50 47 117 378 53 411
Cuts at: 4647 5105 5446 5672 5824 7933 1493
Size: 458 341 226 152 2109 1697
Fragments arranged by size:
2109 1859 1697 458 411 378 341 239
226 152 117 53 50 47
AseI AT'TA_AT
Cuts at: 160 1032 6691 160
Size: 872 5659 1606
Fragments arranged by size:
5659 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8137
AvaI C'yCGr_G
Cuts at: 1870 1943 5462 1870
Size: 73 3519 4545
Fragments arranged by size:
4545 3519 73
BamHI G'GATC_C
Cuts at: 5467 5834 5467
Size: 367 7770
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2515 3758 3948 4421 4698
Size: 325 217 627 728 1243 190 473 277
Cuts at: 4698 5458 6913 618
Size: 760 1455 1842
Fragments arranged by size:
1842 1455 1243 760 728 627 473 325
277 217 190
BanII G_rGCy'C
Cuts at: 1391 2129 3205 5456 1391
Size: 738 1076 2251 4072
Fragments arranged by size:
4072 2251 1076 738
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2555 2625 3119 4879 928
Size: 971 656 70 494 1760 4186
Fragments arranged by size:
4186 1760 971 656 494 70
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6328 6362 1903
Size: 34 4391 34 3678
Fragments arranged by size:
4391 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4072 4922 6029 7239 4072
Size: 850 1107 1210 4970
Fragments arranged by size:
4970 1210 1107 850
BclI T'GATC_A
Cuts at: 1548 2797 3505 4312 1548
Size: 1249 708 807 5373
Fragments arranged by size:
5373 1249 807 708
BfrBI ATG'CAT
Cuts at: 5356 5356
Size: 8137
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2124 6748 136
Size: 107 122 71 631 605 452 4624 1525
Fragments arranged by size:
4624 1525 631 605 452 122 107 71
BglII A'GATC_T
Cuts at: 2649 4113 8132 2649
Size: 1464 4019 2654
Fragments arranged by size:
4019 2654 1464
BlpI GC'TnA_GC
Cuts at: 5554 5554
Size: 8137
Bme1580I G_kGCm'C
Cuts at: 1790 5190 6198 7354 1790
Size: 3400 1008 1156 2573
Fragments arranged by size:
3400 2573 1156 1008
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8137
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3748 5868 6826
Size: 416 53 83 399 24 2331 2120 958
Cuts at: 6826 442
Size: 1753
Fragments arranged by size:
2331 2120 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2192 1056
Size: 1136 7001
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2227 3636 2227
Size: 1409 6728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3153 3535 3893 6797 1239
Size: 1914 382 358 2904 2579
Fragments arranged by size:
2904 2579 1914 382 358
Bpu10I CC'TnA_GC
Cuts at: 5018 5018
Size: 8137
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4922 5111 5416 6262 7127 7425 4922
Size: 189 305 846 865 298 5634
Fragments arranged by size:
5634 865 846 305 298 189
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4000 6800 882
Size: 617 2501 2800 2219
Fragments arranged by size:
2800 2501 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5928 493
Size: 711 4724 2702
Fragments arranged by size:
4724 2702 711
BsaBI GATnn'nnATC
Cuts at: 4890 8131 4890
Size: 3241 4896
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5944 6326 6961 275
Size: 53 83 186 936 4411 382 635 1451
Fragments arranged by size:
4411 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6570 6968 7242 7389 7800 6570
Size: 398 274 147 411 6907
Fragments arranged by size:
6907 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3932 3962 1178
Size: 30 165 30 2529 30 5353
Fragments arranged by size:
5353 2529 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 5068 1186
Size: 3882 4255
BseYI C'CCAG_C
Cuts at: 2751 2859 7340 7836 2751
Size: 108 4481 496 3052
Fragments arranged by size:
4481 3052 496 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2411 1163
Size: 573 675 6889
Fragments arranged by size:
6889 675 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3499 5587 6348 7376 1540
Size: 275 186 1498 2088 761 1028 2301
Fragments arranged by size:
2301 2088 1498 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4129 6198 6283 7354 4129
Size: 2069 85 1071 4912
Fragments arranged by size:
4912 2069 1071 85
BsmI GAATG_Cn'
Cuts at: 5356 5648 5741 5356
Size: 292 93 7752
Fragments arranged by size:
7752 292 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2129 3205 4129 5190 5456 6198 6283
Size: 399 339 1076 924 1061 266 742 85
Cuts at: 6283 7354 1391
Size: 1071 2174
Fragments arranged by size:
2174 1076 1071 1061 924 742 399 339
266 85
BspEI T'CCGG_A
Cuts at: 7800 7800
Size: 8137
BspHI T'CATG_A
Cuts at: 3709 5040 6026 7756 3709
Size: 1331 986 1730 4090
Fragments arranged by size:
4090 1730 1331 986
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5492 844
Size: 4648 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 6024 1394
Size: 429 4201 3507
Fragments arranged by size:
4201 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4647 5188 6632 6806 65
Size: 1183 3399 541 1444 174 1396
Fragments arranged by size:
3399 1444 1396 1183 541 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6781 6968 1700
Size: 110 4971 187 2869
Fragments arranged by size:
4971 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8137
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8137
BssSI C'ACGA_G
Cuts at: 6197 7209 6197
Size: 1012 7125
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8137
BstBI TT'CG_AA
Cuts at: 4945 6941 4945
Size: 1996 6141
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8137
BstXI CCAn_nnnn'nTGG
Cuts at: 2297 4606 2297
Size: 2309 5828
BstYI r'GATC_y
Cuts at: 1972 2649 3013 3020 4113 5467 5834 6219 6236
Size: 677 364 7 1093 1354 367 385 17
Cuts at: 6236 7688 7774 7786 8132 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1354 1093 677 385 367 364
346 86 17 12 7
Bsu36I CC'TnA_GG
Cuts at: 2136 2136
Size: 8137
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7585
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7398
Fragments arranged by size:
7398 683 56
BtsI GCAGTG_nn'
Cuts at: 2044 2340 2972 4802 5653 6447 6467 2044
Size: 296 632 1830 851 794 20 3714
Fragments arranged by size:
3714 1830 851 794 632 296 20
ClaI AT'CG_AT
Cuts at: 1554 3455 1554
Size: 1901 6236
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8102
DraI TTT'AAA
Cuts at: 3121 3370 5307 5441 3121
Size: 249 1937 134 5817
Fragments arranged by size:
5817 1937 249 134
DraIII CAC_nnn'GTG
Cuts at: 2369 2369
Size: 8137
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7144 809
Size: 507 5828 1802
Fragments arranged by size:
5828 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5584 6473 8102
Size: 54 1425 50 275 18 3754 889 1629
Cuts at: 8102 8
Size: 43
Fragments arranged by size:
3754 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5584 1537
Size: 275 3772 4090
Fragments arranged by size:
4090 3772 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4436 4743 6067 1353
Size: 3083 307 1324 3423
Fragments arranged by size:
3423 3083 1324 307
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6724 7098 7244 387
Size: 1064 138 5135 374 146 1280
Fragments arranged by size:
5135 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3153 3535 3893 4023 4287 6200 6797
Size: 393 1521 382 358 130 264 1913 597
Cuts at: 6797 7584 7852 1239
Size: 787 268 1524
Fragments arranged by size:
1913 1524 1521 787 597 393 382 358
268 264 130
EcoRI G'AATT_C
Cuts at: 3352 5105 5446 5824 3352
Size: 1753 341 378 5665
Fragments arranged by size:
5665 1753 378 341
FspI TGC'GCA
Cuts at: 6643 6643
Size: 8137
HaeII r_GCGC'y
Cuts at: 1678 7284 1678
Size: 5606 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2214 2246 3005 3037 3839
Size: 32 135 32 642 32 759 32 802
Cuts at: 3839 3871 3971 4003 6778 6810 6852 6884 1373
Size: 32 100 32 2775 32 42 32 2626
Fragments arranged by size:
2775 2626 802 759 642 135 100 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2338 5481 5727 669
Size: 988 281 400 3143 246 3079
Fragments arranged by size:
3143 3079 988 400 281 246
HindIII A'AGCT_T
Cuts at: 748 3239 748
Size: 2491 5646
HpaI GTT'AAC
Cuts at: 5727 5727
Size: 8137
KpnI G_GTAC'C
Cuts at: 3762 3952 4702 5462 3762
Size: 190 750 760 6437
Fragments arranged by size:
6437 760 750 190
MfeI C'AATT_G
Cuts at: 5736 5736
Size: 8137
MluI A'CGCG_T
Cuts at: 8013 8013
Size: 8137
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2379 4576 7251 7435 1305
Size: 1074 2197 2675 184 2007
Fragments arranged by size:
2675 2197 2007 1074 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6315
Fragments arranged by size:
6315 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3081 3711 5060 5193 6095 6454 6613
Size: 648 1915 630 1349 133 902 359 159
Cuts at: 6613 518
Size: 2042
Fragments arranged by size:
2042 1915 1349 902 648 630 359 159
133
MspA1I CmG'CkG
Cuts at: 1606 5553 6230 7378 7623 1606
Size: 3947 677 1148 245 2120
Fragments arranged by size:
3947 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7585
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8137
NheI G'CTAG_C
Cuts at: 1052 2188 1052
Size: 1136 7001
NotI GC'GGCC_GC
Cuts at: 5584 5584
Size: 8137
NsiI A_TGCA'T
Cuts at: 5358 5358
Size: 8137
NspI r_CATG'y
Cuts at: 1132 1229 2932 5495 7040 1132
Size: 97 1703 2563 1545 2229
Fragments arranged by size:
2563 2229 1703 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3153 1348
Size: 1805 6332
PciI A'CATG_T
Cuts at: 7036 7036
Size: 8137
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2438 1108
Size: 1330 6807
PfoI T'CCnGG_A
Cuts at: 3130 3181 3870 4779 4914 3130
Size: 51 689 909 135 6353
Fragments arranged by size:
6353 909 689 135 51
PmeI GTTT'AAAC
Cuts at: 5441 5441
Size: 8137
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6206 6238 7719 7751 6206
Size: 32 1481 32 6592
Fragments arranged by size:
6592 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2088 1613
Size: 475 7662
PsiI TTA'TAA
Cuts at: 5707 5707
Size: 8137
PstI C_TGCA'G
Cuts at: 830 2347 5489 830
Size: 1517 3142 3478
Fragments arranged by size:
3478 3142 1517
PvuI CG_AT'CG
Cuts at: 2001 3499 2001
Size: 1498 6639
RsrII CG'GwC_CG
Cuts at: 2853 2853
Size: 8137
SalI G'TCGA_C
Cuts at: 1936 5479 1936
Size: 3543 4594
SbfI CC_TGCA'GG
Cuts at: 5489 5489
Size: 8137
ScaI AGT'ACT
Cuts at: 6385 8097 6385
Size: 1712 6425
SfcI C'TryA_G
Cuts at: 826 1045 2343 3058 5485 6620 7301 7492 826
Size: 219 1298 715 2427 1135 681 191 1471
Fragments arranged by size:
2427 1471 1298 1135 715 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8137
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8137
SmaI CCC'GGG
Cuts at: 1872 5464 1872
Size: 3592 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4901 5090 5395 6241 7142 7404
Size: 197 926 2958 189 305 846 901 262
Cuts at: 7404 820
Size: 1553
Fragments arranged by size:
2958 1553 926 901 846 305 262 197
189
SnaBI TAC'GTA
Cuts at: 493 5928 493
Size: 5435 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8137
SphI G_CATG'C
Cuts at: 1132 5495 1132
Size: 4363 3774
SspI AAT'ATT
Cuts at: 5 52 2218 4603 6061 5
Size: 47 2166 2385 1458 2081
Fragments arranged by size:
2385 2166 2081 1458 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8137
StyI C'CwwG_G
Cuts at: 513 1065 2290 3944 5576 513
Size: 552 1225 1654 1632 3074
Fragments arranged by size:
3074 1654 1632 1225 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2841 3730 3996 4987 6164 6181 6334
Size: 1255 1261 889 266 991 1177 17 153
Cuts at: 6334 6519 6964 325
Size: 185 445 1498
Fragments arranged by size:
1498 1261 1255 1177 991 889 445 266
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6383 8095 96
Size: 274 80 33 51 1443 4406 1712 138
Fragments arranged by size:
4406 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4035 4067 4137 4169 4572 4604 8097 8129 4035
Size: 32 70 32 403 32 3493 32 4043
Fragments arranged by size:
4043 3493 403 70 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2228 1316
Size: 912 7225
XbaI T'CTAG_A
Cuts at: 5473 5473
Size: 8137
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3259 3688 4765 3259
Size: 429 1077 6631
Fragments arranged by size:
6631 1077 429
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8137
XmaI C'CCGG_G
Cuts at: 1870 5462 1870
Size: 3592 4545
XmnI GAAnn'nnTTC
Cuts at: 2499 2552 6266 2499
Size: 53 3714 4370
Fragments arranged by size:
4370 3714 53
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5945 276
Size: 53 83 186 936 4411 2468
Fragments arranged by size:
4411 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI RsrII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI