Restriction map for
pFN21AB5656
(Circular) (Six-base) MAPSORT of: pFN21AB5656.seq Check: 6428 from: 1 to: 5746
pFN21AB5656 5746 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2863 2863
Size: 5746
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3556 278
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3089 1937
Size: 1152 4594
Acc65I G'GTAC_C
Cuts at: 3067 3067
Size: 5746
AclI AA'CG_TT
Cuts at: 1563 3873 4246 1563
Size: 2310 373 3063
Fragments arranged by size:
3063 2310 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2171 2524 3809 5193 5461 1632
Size: 539 353 1285 1384 268 1917
Fragments arranged by size:
1917 1384 1285 539 353 268
AfeI AGC'GCT
Cuts at: 2456 2456
Size: 5746
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5549
AflIII A'CryG_T
Cuts at: 1203 4645 5622 1203
Size: 3442 977 1327
Fragments arranged by size:
3442 1327 977
AgeI A'CCGG_T
Cuts at: 4577 4577
Size: 5746
AhdI GACnn_n'nnGTC
Cuts at: 2384 4475 2384
Size: 2091 3655
AleI CACnn'nnGTG
Cuts at: 1166 2792 1166
Size: 1626 4120
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2921 1184
Size: 1737 4009
AlwNI CAG_nnn'CTG
Cuts at: 2857 2878 3489 5061 2857
Size: 21 611 1572 3542
Fragments arranged by size:
3542 1572 611 21
ApaLI G'TGCA_C
Cuts at: 3803 4959 3803
Size: 1156 4590
ApoI r'AATT_y
Cuts at: 1493 2153 2675 3055 3281 3433 5542 1493
Size: 660 522 380 226 152 2109 1697
Fragments arranged by size:
2109 1697 660 522 380 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4300 160
Size: 872 3268 1606
Fragments arranged by size:
3268 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
AvaI C'yCGr_G
Cuts at: 1870 1943 2697 3071 1870
Size: 73 754 374 4545
Fragments arranged by size:
4545 754 374 73
BamHI G'GATC_C
Cuts at: 3076 3443 3076
Size: 367 5379
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2126 3067 4522 618
Size: 325 217 627 339 941 1455 1842
Fragments arranged by size:
1842 1455 941 627 339 325 217
BanII G_rGCy'C
Cuts at: 1391 2712 3065 1391
Size: 1321 353 4072
Fragments arranged by size:
4072 1321 353
BbeI G_GCGC'C
Cuts at: 2130 2130
Size: 5746
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2061 2190 2530 928
Size: 971 162 129 340 4144
Fragments arranged by size:
4144 971 340 162 129
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3937 3971 1903
Size: 34 2000 34 3678
Fragments arranged by size:
3678 2000 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3638 4848 3638
Size: 1210 4536
BclI T'GATC_A
Cuts at: 1548 2611 1548
Size: 1063 4683
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2600 4357 136
Size: 107 122 71 631 605 928 1757 1525
Fragments arranged by size:
1757 1525 928 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5741 5741
Size: 5746
BlpI GC'TnA_GC
Cuts at: 3163 3163
Size: 5746
Bme1580I G_kGCm'C
Cuts at: 1790 3807 4963 1790
Size: 2017 1156 2573
Fragments arranged by size:
2573 2017 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5746
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2100 3477 4435
Size: 416 53 83 399 24 683 1377 958
Cuts at: 4435 442
Size: 1753
Fragments arranged by size:
1753 1377 958 683 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5746
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2185 2185
Size: 5746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2825 2879 4406 1239
Size: 1586 54 1527 2579
Fragments arranged by size:
2579 1586 1527 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2157 3871 4736 5034 2157
Size: 1714 865 298 2869
Fragments arranged by size:
2869 1714 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2371 2855 4409 882
Size: 617 872 484 1554 2219
Fragments arranged by size:
2219 1554 872 617 484
BsaAI yAC'GTr
Cuts at: 493 1204 2773 3537 493
Size: 711 1569 764 2702
Fragments arranged by size:
2702 1569 764 711
BsaBI GATnn'nnATC
Cuts at: 5740 5740
Size: 5746
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2127 3553 3935 4570
Size: 53 83 186 936 594 1426 382 635
Cuts at: 4570 275
Size: 1451
Fragments arranged by size:
1451 1426 936 635 594 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4179 4577 4851 4998 5409 4179
Size: 398 274 147 411 4516
Fragments arranged by size:
4516 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5521
Fragments arranged by size:
5521 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2271 2759 1186
Size: 1085 488 4173
Fragments arranged by size:
4173 1085 488
BseYI C'CCAG_C
Cuts at: 4949 5445 4949
Size: 496 5250
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2948 1163
Size: 573 1212 3961
Fragments arranged by size:
3961 1212 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3043 3196 3957 4985 1540
Size: 275 186 1042 153 761 1028 2301
Fragments arranged by size:
2301 1042 1028 761 275 186 153
BsiHKAI G_wGCw'C
Cuts at: 2712 3807 3892 4963 2712
Size: 1095 85 1071 3495
Fragments arranged by size:
3495 1095 1071 85
BsmI GAATG_Cn'
Cuts at: 2758 3257 3350 2758
Size: 499 93 5154
Fragments arranged by size:
5154 499 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2712 3065 3807 3892 4963 1391
Size: 399 922 353 742 85 1071 2174
Fragments arranged by size:
2174 1071 922 742 399 353 85
BspEI T'CCGG_A
Cuts at: 5409 5409
Size: 5746
BspHI T'CATG_A
Cuts at: 2343 2608 2614 3635 5365 2343
Size: 265 6 1021 1730 2724
Fragments arranged by size:
2724 1730 1021 265 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2863 3101 844
Size: 2019 238 3489
Fragments arranged by size:
3489 2019 238
BsrBI CCG'CTC
Cuts at: 1394 1823 2827 3633 1394
Size: 429 1004 806 3507
Fragments arranged by size:
3507 1004 806 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2224 2311 2425 4241 4415 65
Size: 1183 976 87 114 1816 174 1396
Fragments arranged by size:
1816 1396 1183 976 174 114 87
BsrFI r'CCGG_y
Cuts at: 1700 1810 4390 4577 1700
Size: 110 2580 187 2869
Fragments arranged by size:
2869 2580 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5746
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5746
BssSI C'ACGA_G
Cuts at: 3806 4818 3806
Size: 1012 4734
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5746
BstBI TT'CG_AA
Cuts at: 4550 4550
Size: 5746
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5746
BstYI r'GATC_y
Cuts at: 1972 2164 3076 3443 3828 3845 5297 5383 5395
Size: 192 912 367 385 17 1452 86 12
Cuts at: 5395 5741 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 912 385 367 346 192 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2442 2442
Size: 5746
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4255
Fragments arranged by size:
4255 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5007
Fragments arranged by size:
5007 683 56
BtsI GCAGTG_nn'
Cuts at: 2799 3262 4056 4076 2799
Size: 463 794 20 4469
Fragments arranged by size:
4469 794 463 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5711
DraI TTT'AAA
Cuts at: 3050 3050
Size: 5746
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4753 809
Size: 507 3437 1802
Fragments arranged by size:
3437 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3193 4082 5711
Size: 54 1425 50 275 18 1363 889 1629
Cuts at: 5711 8
Size: 43
Fragments arranged by size:
1629 1425 1363 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3193 1537
Size: 275 1381 4090
Fragments arranged by size:
4090 1381 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2298 3676 1353
Size: 945 1378 3423
Fragments arranged by size:
3423 1378 945
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2702 4333 4707 4853 387
Size: 1064 138 1113 1631 374 146 1280
Fragments arranged by size:
1631 1280 1113 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2710 2710
Size: 5746
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 2524 2825 2879 3809 4406 5193
Size: 393 539 353 301 54 930 597 787
Cuts at: 5193 5461 1239
Size: 268 1524
Fragments arranged by size:
1524 930 787 597 539 393 353 301
268 54
EcoNI CCTnn'n_nnAGG
Cuts at: 2870 2870
Size: 5746
EcoRI G'AATT_C
Cuts at: 2675 3055 3433 2675
Size: 380 378 4988
Fragments arranged by size:
4988 380 378
FspI TGC'GCA
Cuts at: 2786 4252 2786
Size: 1466 4280
FspAI rTGC'GCAy
Cuts at: 2786 2786
Size: 5746
HaeII r_GCGC'y
Cuts at: 1678 2130 2458 4893 1678
Size: 452 328 2435 2531
Fragments arranged by size:
2531 2435 452 328
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2062 2094 2370 2402 4387
Size: 32 135 32 490 32 276 32 1985
Cuts at: 4387 4419 4461 4493 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1985 490 276 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3090 3336 669
Size: 988 281 1152 246 3079
Fragments arranged by size:
3079 1152 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5746
HpaI GTT'AAC
Cuts at: 3336 3336
Size: 5746
KasI G'GCGC_C
Cuts at: 2126 2126
Size: 5746
KpnI G_GTAC'C
Cuts at: 3071 3071
Size: 5746
MfeI C'AATT_G
Cuts at: 3345 3345
Size: 5746
MluI A'CGCG_T
Cuts at: 5622 5622
Size: 5746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2754 4860 5044 1305
Size: 1449 2106 184 2007
Fragments arranged by size:
2106 2007 1449 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3924
Fragments arranged by size:
3924 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2235 2408 2613 2792 3704 4063 4222
Size: 648 1069 173 205 179 912 359 159
Cuts at: 4222 518
Size: 2042
Fragments arranged by size:
2042 1069 912 648 359 205 179 173
159
MspA1I CmG'CkG
Cuts at: 1606 3162 3839 4987 5232 1606
Size: 1556 677 1148 245 2120
Fragments arranged by size:
2120 1556 1148 677 245
NarI GG'CG_CC
Cuts at: 2127 2127
Size: 5746
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4255
Fragments arranged by size:
4255 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5746
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5746
NotI GC'GGCC_GC
Cuts at: 3193 3193
Size: 5746
NspI r_CATG'y
Cuts at: 1132 1229 2785 2902 3104 4649 1132
Size: 97 1556 117 202 1545 2229
Fragments arranged by size:
2229 1556 1545 202 117 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5746
PciI A'CATG_T
Cuts at: 4645 4645
Size: 5746
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5746
PfoI T'CCnGG_A
Cuts at: 2820 2820
Size: 5746
PmeI GTTT'AAAC
Cuts at: 3050 3050
Size: 5746
PmlI CAC'GTG
Cuts at: 2773 2773
Size: 5746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3815 3847 5328 5360 3815
Size: 32 1481 32 4201
Fragments arranged by size:
4201 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5746
PsiI TTA'TAA
Cuts at: 3316 3316
Size: 5746
PstI C_TGCA'G
Cuts at: 830 3098 830
Size: 2268 3478
PvuI CG_AT'CG
Cuts at: 2001 3043 2001
Size: 1042 4704
SacI G_AGCT'C
Cuts at: 2712 2712
Size: 5746
SalI G'TCGA_C
Cuts at: 1936 3088 1936
Size: 1152 4594
SbfI CC_TGCA'GG
Cuts at: 3098 3098
Size: 5746
ScaI AGT'ACT
Cuts at: 3994 5706 3994
Size: 1712 4034
SfcI C'TryA_G
Cuts at: 826 1045 2928 3012 3094 4229 4910 5101 826
Size: 219 1883 84 82 1135 681 191 1471
Fragments arranged by size:
1883 1471 1135 681 219 191 84 82
SfoI GGC'GCC
Cuts at: 2128 2128
Size: 5746
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5746
SmaI CCC'GGG
Cuts at: 1872 3073 1872
Size: 1201 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2172 3850 4751 5013 820
Size: 197 926 229 1678 901 262 1553
Fragments arranged by size:
1678 1553 926 901 262 229 197
SnaBI TAC'GTA
Cuts at: 493 3537 493
Size: 3044 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5746
SphI G_CATG'C
Cuts at: 1132 2785 2902 3104 1132
Size: 1653 117 202 3774
Fragments arranged by size:
3774 1653 202 117
SspI AAT'ATT
Cuts at: 5 52 3670 5
Size: 47 3618 2081
Fragments arranged by size:
3618 2081 47
StuI AGG'CCT
Cuts at: 1517 2528 1517
Size: 1011 4735
StyI C'CwwG_G
Cuts at: 513 1065 2004 2064 3185 513
Size: 552 939 60 1121 3074
Fragments arranged by size:
3074 1121 939 552 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2118 2326 3773 3790 3943 4128 4573
Size: 1255 538 208 1447 17 153 185 445
Cuts at: 4573 325
Size: 1498
Fragments arranged by size:
1498 1447 1255 538 445 208 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3992 5704 96
Size: 274 80 33 51 1443 2015 1712 138
Fragments arranged by size:
2015 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2131 2163 5706 5738 2131
Size: 32 3543 32 2139
Fragments arranged by size:
3543 2139 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5746
XbaI T'CTAG_A
Cuts at: 3082 3082
Size: 5746
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5746
XmaI C'CCGG_G
Cuts at: 1870 3071 1870
Size: 1201 4545
XmnI GAAnn'nnTTC
Cuts at: 2364 3875 2364
Size: 1511 4235
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3554 276
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I DraIII EcoO109I EcoRV FalI
FseI NaeI NgoMIV NruI NsiI PacI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI
SfiI SrfI SwaI XcmI