Restriction map for
pFN21AB5655
(Circular) (Six-base) MAPSORT of: pFN21AB5655.seq Check: 2516 from: 1 to: 8389
pFN21AB5655 8389 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2284 2284
Size: 8389
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6199 278
Size: 53 83 186 936 4663 2468
Fragments arranged by size:
4663 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3644 5732 1937
Size: 1707 2088 4594
Fragments arranged by size:
4594 2088 1707
Acc65I G'GTAC_C
Cuts at: 5710 5710
Size: 8389
AclI AA'CG_TT
Cuts at: 1563 6516 6889 1563
Size: 4953 373 3063
Fragments arranged by size:
4953 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2115 2247 2414 3891 4865 6452 7836 8104
Size: 483 132 167 1477 974 1587 1384 268
Cuts at: 8104 1632
Size: 1917
Fragments arranged by size:
1917 1587 1477 1384 974 483 268 167
132
AflII C'TTAA_G
Cuts at: 820 1017 3824 5045 820
Size: 197 2807 1221 4164
Fragments arranged by size:
4164 2807 1221 197
AflIII A'CryG_T
Cuts at: 1203 3422 3639 3786 3847 7288 8265 1203
Size: 2219 217 147 61 3441 977 1327
Fragments arranged by size:
3441 2219 1327 977 217 147 61
AgeI A'CCGG_T
Cuts at: 2726 7220 2726
Size: 4494 3895
AhdI GACnn_n'nnGTC
Cuts at: 4600 7118 4600
Size: 2518 5871
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8389
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8389
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3056 3088 3056
Size: 32 8357
AlwNI CAG_nnn'CTG
Cuts at: 3608 6132 7704 3608
Size: 2524 1572 4293
Fragments arranged by size:
4293 2524 1572
ApaLI G'TGCA_C
Cuts at: 2271 6446 7602 2271
Size: 4175 1156 3058
Fragments arranged by size:
4175 3058 1156
ApoI r'AATT_y
Cuts at: 1493 2423 2605 3113 3192 3815 3841 4069 4573
Size: 930 182 508 79 623 26 228 504
Cuts at: 4573 4702 4898 5411 5418 5698 5924 6076 8185
Size: 129 196 513 7 280 226 152 2109
Cuts at: 8185 1493
Size: 1697
Fragments arranged by size:
2109 1697 930 623 513 508 504 280
228 226 196 182 152 129 79 26
7
AseI AT'TA_AT
Cuts at: 160 1032 3153 6943 160
Size: 872 2121 3790 1606
Fragments arranged by size:
3790 2121 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8389
AvaI C'yCGr_G
Cuts at: 1870 1943 2178 5714 1870
Size: 73 235 3536 4545
Fragments arranged by size:
4545 3536 235 73
BamHI G'GATC_C
Cuts at: 2732 5719 6086 2732
Size: 2987 367 5035
Fragments arranged by size:
5035 2987 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5710 7165 618
Size: 325 217 627 3923 1455 1842
Fragments arranged by size:
3923 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2539 2857 5708 1391
Size: 1148 318 2851 4072
Fragments arranged by size:
4072 2851 1148 318
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2120 2228 2235 3789 928
Size: 971 221 108 7 1554 5528
Fragments arranged by size:
5528 1554 971 221 108 7
BbvCI CC'TCA_GC
Cuts at: 2280 2555 2280
Size: 275 8114
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3332 3366 6580 6614 1903
Size: 34 1395 34 3214 34 3678
Fragments arranged by size:
3678 3214 1395 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2819 6281 7491 2819
Size: 3462 1210 3717
Fragments arranged by size:
3717 3462 1210
BclI T'GATC_A
Cuts at: 1548 2980 1548
Size: 1432 6957
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7000 136
Size: 107 122 71 631 605 5328 1525
Fragments arranged by size:
5328 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2747 8384 2747
Size: 5637 2752
BlpI GC'TnA_GC
Cuts at: 2925 5806 2925
Size: 2881 5508
Bme1580I G_kGCm'C
Cuts at: 1790 2275 5168 6450 7606 1790
Size: 485 2893 1282 1156 2573
Fragments arranged by size:
2893 2573 1282 1156 485
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8389
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5577 6120 7078
Size: 416 53 83 399 24 4160 543 958
Cuts at: 7078 442
Size: 1753
Fragments arranged by size:
4160 1753 958 543 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2370 1056
Size: 1314 7075
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3995 3995
Size: 8389
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2193 2406 2511 2538 3585 5250 7049 1239
Size: 954 213 105 27 1047 1665 1799 2579
Fragments arranged by size:
2579 1799 1665 1047 954 213 105 27
Bpu10I CC'TnA_GC
Cuts at: 2280 2555 2280
Size: 275 8114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3478 3983 4914 6514 7379 7677 3478
Size: 505 931 1600 865 298 4190
Fragments arranged by size:
4190 1600 931 865 505 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7052 882
Size: 617 5553 2219
Fragments arranged by size:
5553 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5169 5328 6180 493
Size: 711 3965 159 852 2702
Fragments arranged by size:
3965 2702 852 711 159
BsaBI GATnn'nnATC
Cuts at: 8383 8383
Size: 8389
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6196 6578 7213 275
Size: 53 83 186 936 4663 382 635 1451
Fragments arranged by size:
4663 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2726 6822 7220 7494 7641 8052 2726
Size: 4096 398 274 147 411 3063
Fragments arranged by size:
4096 3063 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2239 2269 2803 2833 3056
Size: 30 165 30 836 30 534 30 223
Cuts at: 3056 3086 3746 3776 1178
Size: 30 660 30 5791
Fragments arranged by size:
5791 836 660 534 223 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2931 3064 5138 1186
Size: 1745 133 2074 4437
Fragments arranged by size:
4437 2074 1745 133
BseYI C'CCAG_C
Cuts at: 3321 7592 8088 3321
Size: 4271 496 3622
Fragments arranged by size:
4271 3622 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5215 1163
Size: 573 3479 4337
Fragments arranged by size:
4337 3479 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4970 5839 6600 7628 1540
Size: 275 186 2969 869 761 1028 2301
Fragments arranged by size:
2969 2301 1028 869 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2275 3478 5473 6450 6535 7606 2275
Size: 1203 1995 977 85 1071 3058
Fragments arranged by size:
3058 1995 1203 1071 977 85
BsmI GAATG_Cn'
Cuts at: 2077 3501 3601 4834 5900 5993 2077
Size: 1424 100 1233 1066 93 4473
Fragments arranged by size:
4473 1424 1233 1066 100 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3745 3745
Size: 8389
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2275 2539 2857 3478 5168 5473 5708
Size: 399 485 264 318 621 1690 305 235
Cuts at: 5708 6450 6535 7606 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1690 1071 742 621 485 399 318
305 264 235 85
BspEI T'CCGG_A
Cuts at: 8052 8052
Size: 8389
BspHI T'CATG_A
Cuts at: 6278 8008 6278
Size: 1730 6659
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2284 4415 5744 844
Size: 1440 2131 1329 3489
Fragments arranged by size:
3489 2131 1440 1329
BsrBI CCG'CTC
Cuts at: 1394 1823 6276 1394
Size: 429 4453 3507
Fragments arranged by size:
4453 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3006 5021 6884 7058 65
Size: 1183 1758 2015 1863 174 1396
Fragments arranged by size:
2015 1863 1758 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2726 7033 7220 1700
Size: 110 916 4307 187 2869
Fragments arranged by size:
4307 2869 916 187 110
BsrGI T'GTAC_A
Cuts at: 96 2644 96
Size: 2548 5841
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8389
BssSI C'ACGA_G
Cuts at: 6449 7461 6449
Size: 1012 7377
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2167 5315 794
Size: 1373 3148 3868
Fragments arranged by size:
3868 3148 1373
BstBI TT'CG_AA
Cuts at: 2610 7193 2610
Size: 4583 3806
BstEII G'GTnAC_C
Cuts at: 1184 2951 1184
Size: 1767 6622
BstYI r'GATC_y
Cuts at: 1972 2732 2747 3945 4284 5282 5719 6086 6471
Size: 760 15 1198 339 998 437 367 385
Cuts at: 6471 6488 7940 8026 8038 8384 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1198 998 760 437 385 367
346 339 86 17 15 12
Bsu36I CC'TnA_GG
Cuts at: 2094 5121 2094
Size: 3027 5362
BtgI C'CryG_G
Cuts at: 513 1065 2540 5301 513
Size: 552 1475 2761 3601
Fragments arranged by size:
3601 2761 1475 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7650
Fragments arranged by size:
7650 683 56
BtsI GCAGTG_nn'
Cuts at: 2249 4660 5905 6699 6719 2249
Size: 2411 1245 794 20 3919
Fragments arranged by size:
3919 2411 1245 794 20
ClaI AT'CG_AT
Cuts at: 1554 4966 1554
Size: 3412 4977
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8354
DraI TTT'AAA
Cuts at: 3190 3814 5693 3190
Size: 624 1879 5886
Fragments arranged by size:
5886 1879 624
DraIII CAC_nnn'GTG
Cuts at: 3394 3394
Size: 8389
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2684 7396 809
Size: 507 1368 4712 1802
Fragments arranged by size:
4712 1802 1368 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5836 6725 8354
Size: 54 1425 50 275 18 4006 889 1629
Cuts at: 8354 8
Size: 43
Fragments arranged by size:
4006 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5836 1537
Size: 275 4024 4090
Fragments arranged by size:
4090 4024 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3018 3129 3236 3831 4689 6319 1353
Size: 1665 111 107 595 858 1630 3423
Fragments arranged by size:
3423 1665 1630 858 595 111 107
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2207 6976 7350 7496 387
Size: 1064 138 618 4769 374 146 1280
Fragments arranged by size:
4769 1280 1064 618 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2115 2193 2247 2406 2414 2511 2538
Size: 393 483 78 54 159 8 97 27
Cuts at: 2538 3585 3891 4865 5250 6452 7049 7836 8104
Size: 1047 306 974 385 1202 597 787 268
Cuts at: 8104 1239
Size: 1524
Fragments arranged by size:
1524 1202 1047 974 787 597 483 393
385 306 268 159 97 78 54 27
8
EcoO109I rG'GnC_Cy
Cuts at: 2711 3259 2711
Size: 548 7841
EcoRI G'AATT_C
Cuts at: 5418 5698 6076 5418
Size: 280 378 7731
Fragments arranged by size:
7731 378 280
EcoRV GAT'ATC
Cuts at: 2760 2760
Size: 8389
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2252 3084 2252
Size: 832 7557
FspI TGC'GCA
Cuts at: 6895 6895
Size: 8389
HaeII r_GCGC'y
Cuts at: 1678 7536 1678
Size: 5858 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2010 2042 2090 2122 2749
Size: 32 135 32 438 32 48 32 627
Cuts at: 2749 2781 2803 2835 3626 3658 3715 3746 3747
Size: 32 22 32 791 32 57 31 1
Cuts at: 3747 3776 3778 3808 4027 4059 4586 4618 7030
Size: 29 2 30 219 32 527 32 2412
Cuts at: 7030 7062 7104 7136 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2412 791 627 527 438 219 135
57 48 42 32 32 32 32 32
32 32 32 32 32 32 31 30
29 22 2 1
HincII GTy'rAC
Cuts at: 669 1657 1938 2846 5733 5979 669
Size: 988 281 908 2887 246 3079
Fragments arranged by size:
3079 2887 988 908 281 246
HindIII A'AGCT_T
Cuts at: 748 5240 5510 748
Size: 4492 270 3627
Fragments arranged by size:
4492 3627 270
HpaI GTT'AAC
Cuts at: 5979 5979
Size: 8389
KpnI G_GTAC'C
Cuts at: 5714 5714
Size: 8389
MfeI C'AATT_G
Cuts at: 5988 5988
Size: 8389
MluI A'CGCG_T
Cuts at: 8265 8265
Size: 8389
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2461 2488 4482 4701 7503 7687 1305
Size: 1156 27 1994 219 2802 184 2007
Fragments arranged by size:
2802 2007 1994 1156 219 184 27
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6567
Fragments arranged by size:
6567 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5328 6347 6706 6865 518
Size: 648 4162 1019 359 159 2042
Fragments arranged by size:
4162 2042 1019 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2906 3605 5805 6482 7630 7875 1606
Size: 1300 699 2200 677 1148 245 2120
Fragments arranged by size:
2200 2120 1300 1148 699 677 245
NcoI C'CATG_G
Cuts at: 513 1065 5301 513
Size: 552 4236 3601
Fragments arranged by size:
4236 3601 552
NdeI CA'TA_TG
Cuts at: 387 3997 387
Size: 3610 4779
NheI G'CTAG_C
Cuts at: 1052 2366 1052
Size: 1314 7075
NotI GC'GGCC_GC
Cuts at: 5836 5836
Size: 8389
NspI r_CATG'y
Cuts at: 1132 1229 3211 3426 3643 3790 3851 5747 7292
Size: 97 1982 215 217 147 61 1896 1545
Cuts at: 7292 1132
Size: 2229
Fragments arranged by size:
2229 1982 1896 1545 217 215 147 97
61
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8389
PciI A'CATG_T
Cuts at: 3422 3639 3786 3847 7288 3422
Size: 217 147 61 3441 4523
Fragments arranged by size:
4523 3441 217 147 61
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5405 1108
Size: 4297 4092
PfoI T'CCnGG_A
Cuts at: 2383 2821 2383
Size: 438 7951
PmeI GTTT'AAAC
Cuts at: 5693 5693
Size: 8389
PmlI CAC'GTG
Cuts at: 5169 5169
Size: 8389
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3056 3088 6458 6490 7971 8003 3056
Size: 32 3370 32 1481 32 3442
Fragments arranged by size:
3442 3370 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2711 3259 2711
Size: 548 7841
PshAI GACnn'nnGTC
Cuts at: 1613 4370 1613
Size: 2757 5632
PsiI TTA'TAA
Cuts at: 4208 5959 4208
Size: 1751 6638
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4466 4498 5176 5208 4466
Size: 32 678 32 7647
Fragments arranged by size:
7647 678 32 32
PstI C_TGCA'G
Cuts at: 830 3021 5207 5741 830
Size: 2191 2186 534 3478
Fragments arranged by size:
3478 2191 2186 534
PvuI CG_AT'CG
Cuts at: 2001 4970 2001
Size: 2969 5420
PvuII CAG'CTG
Cuts at: 2906 3605 2906
Size: 699 7690
SalI G'TCGA_C
Cuts at: 1936 5731 1936
Size: 3795 4594
SapI GCTCTTCn'nnn_
Cuts at: 3236 4689 3236
Size: 1453 6936
SbfI CC_TGCA'GG
Cuts at: 5741 5741
Size: 8389
ScaI AGT'ACT
Cuts at: 4264 6637 8349 4264
Size: 2373 1712 4304
Fragments arranged by size:
4304 2373 1712
SexAI A'CCwGG_T
Cuts at: 2651 2682 5344 2651
Size: 31 2662 5696
Fragments arranged by size:
5696 2662 31
SfcI C'TryA_G
Cuts at: 826 1045 2773 3017 3219 5137 5203 5737 6872
Size: 219 1728 244 202 1918 66 534 1135
Cuts at: 6872 7553 7744 826
Size: 681 191 1471
Fragments arranged by size:
1918 1728 1471 1135 681 534 244 219
202 191 66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8389
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8389
SmaI CCC'GGG
Cuts at: 1872 5716 1872
Size: 3844 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3493 3824 3962 4929 5045 6493
Size: 197 926 1550 331 138 967 116 1448
Cuts at: 6493 7394 7656 820
Size: 901 262 1553
Fragments arranged by size:
1553 1550 1448 967 926 901 331 262
197 138 116
SnaBI TAC'GTA
Cuts at: 493 5328 6180 493
Size: 4835 852 2702
Fragments arranged by size:
4835 2702 852
SpeI A'CTAG_T
Cuts at: 152 4049 152
Size: 3897 4492
SphI G_CATG'C
Cuts at: 1132 5747 1132
Size: 4615 3774
SspI AAT'ATT
Cuts at: 5 52 3037 5533 6313 5
Size: 47 2985 2496 780 2081
Fragments arranged by size:
2985 2496 2081 780 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8389
StyI C'CwwG_G
Cuts at: 513 1065 5301 5828 513
Size: 552 4236 527 3074
Fragments arranged by size:
4236 3074 552 527
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5314 6416 6433 6586 6771 7216 325
Size: 1255 3734 1102 17 153 185 445 1498
Fragments arranged by size:
3734 1498 1255 1102 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2644 3157 3550
Size: 274 80 33 51 1443 667 513 393
Cuts at: 3550 3636 4120 4262 5448 6635 8347 96
Size: 86 484 142 1186 1187 1712 138
Fragments arranged by size:
1712 1443 1187 1186 667 513 484 393
274 142 138 86 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2798 2830 5475 5507 8349 8381 2798
Size: 32 2645 32 2842 32 2806
Fragments arranged by size:
2842 2806 2645 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8389
XbaI T'CTAG_A
Cuts at: 3478 5725 3478
Size: 2247 6142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2671 2671
Size: 8389
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8389
XmaI C'CCGG_G
Cuts at: 1870 5714 1870
Size: 3844 4545
XmnI GAAnn'nnTTC
Cuts at: 6518 6518
Size: 8389
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6197 276
Size: 53 83 186 936 4663 2468
Fragments arranged by size:
4663 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BstXI BstZ17I EcoICRI EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PspOMI PspXI RsrII
SacI SacII SanDI SfiI SfoI SrfI SwaI