Restriction map for
pFN21AB5646
(Circular) (Six-base) MAPSORT of: pFN21AB5646.seq Check: 1669 from: 1 to: 6169
pFN21AB5646 6169 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3979 278
Size: 53 83 186 936 2443 2468
Fragments arranged by size:
2468 2443 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3512 1937
Size: 1575 4594
Acc65I G'GTAC_C
Cuts at: 3490 3490
Size: 6169
AclI AA'CG_TT
Cuts at: 1563 4296 4669 1563
Size: 2733 373 3063
Fragments arranged by size:
3063 2733 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3282 4232 5616 5884 1632
Size: 1650 950 1384 268 1917
Fragments arranged by size:
1917 1650 1384 950 268
AflII C'TTAA_G
Cuts at: 820 1017 2719 820
Size: 197 1702 4270
Fragments arranged by size:
4270 1702 197
AflIII A'CryG_T
Cuts at: 1203 2335 5068 6045 1203
Size: 1132 2733 977 1327
Fragments arranged by size:
2733 1327 1132 977
AgeI A'CCGG_T
Cuts at: 5000 5000
Size: 6169
AhdI GACnn_n'nnGTC
Cuts at: 4898 4898
Size: 6169
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6169
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6169
AlwNI CAG_nnn'CTG
Cuts at: 3912 5484 3912
Size: 1572 4597
ApaLI G'TGCA_C
Cuts at: 4226 5382 4226
Size: 1156 5013
ApoI r'AATT_y
Cuts at: 1493 2187 2394 2705 3478 3704 3856 5965 1493
Size: 694 207 311 773 226 152 2109 1697
Fragments arranged by size:
2109 1697 773 694 311 226 207 152
AseI AT'TA_AT
Cuts at: 160 1032 2634 2927 4723 160
Size: 872 1602 293 1796 1606
Fragments arranged by size:
1796 1606 1602 872 293
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6169
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 2054 2084 3453 3494 1870
Size: 73 93 18 30 1369 41 4545
Fragments arranged by size:
4545 1369 93 73 41 30 18
BamHI G'GATC_C
Cuts at: 3055 3499 3866 3055
Size: 444 367 5358
Fragments arranged by size:
5358 444 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2467 3490 4945 618
Size: 325 217 627 680 1023 1455 1842
Fragments arranged by size:
1842 1455 1023 680 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2231 3488 1391
Size: 840 1257 4072
Fragments arranged by size:
4072 1257 840
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5198
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4360 4394 1903
Size: 34 2423 34 3678
Fragments arranged by size:
3678 2423 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4061 5271 4061
Size: 1210 4959
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6169
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4780 136
Size: 107 122 71 631 605 337 2771 1525
Fragments arranged by size:
2771 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6164 6164
Size: 6169
BlpI GC'TnA_GC
Cuts at: 2075 3586 2075
Size: 1511 4658
Bme1580I G_kGCm'C
Cuts at: 1790 4230 5386 1790
Size: 2440 1156 2573
Fragments arranged by size:
2573 2440 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6169
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3900 4858 442
Size: 416 53 83 399 24 2483 958 1753
Fragments arranged by size:
2483 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2384 1056
Size: 1328 4841
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 6169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2313 2407 2886 4829 1239
Size: 1074 94 479 1943 2579
Fragments arranged by size:
2579 1943 1074 479 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2250 2668 4294 5159 5457 2250
Size: 418 1626 865 298 2962
Fragments arranged by size:
2962 1626 865 418 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4832 882
Size: 617 3333 2219
Fragments arranged by size:
3333 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3960 493
Size: 711 2756 2702
Fragments arranged by size:
2756 2702 711
BsaBI GATnn'nnATC
Cuts at: 6163 6163
Size: 6169
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3976 4358 4993 275
Size: 53 83 186 936 2443 382 635 1451
Fragments arranged by size:
2443 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4602 5000 5274 5421 5832 4602
Size: 398 274 147 411 4939
Fragments arranged by size:
4939 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2979 3009 1178
Size: 30 165 30 1576 30 4338
Fragments arranged by size:
4338 1576 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3104 3312 1186
Size: 1918 208 4043
Fragments arranged by size:
4043 1918 208
BseYI C'CCAG_C
Cuts at: 2740 5372 5868 2740
Size: 2632 496 3041
Fragments arranged by size:
3041 2632 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2752 2829 3063 1163
Size: 573 1016 77 234 4269
Fragments arranged by size:
4269 1016 573 234 77
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3619 4380 5408 1540
Size: 275 186 1618 761 1028 2301
Fragments arranged by size:
2301 1618 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2076 4230 4315 5386 2076
Size: 2154 85 1071 2859
Fragments arranged by size:
2859 2154 1071 85
BsmI GAATG_Cn'
Cuts at: 3680 3773 3680
Size: 93 6076
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2231 3488 4230 4315 5386 1391
Size: 399 286 155 1257 742 85 1071 2174
Fragments arranged by size:
2174 1257 1071 742 399 286 155 85
BspEI T'CCGG_A
Cuts at: 5832 5832
Size: 6169
BspHI T'CATG_A
Cuts at: 4058 5788 4058
Size: 1730 4439
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3524 844
Size: 2680 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2017 4056 1394
Size: 429 194 2039 3507
Fragments arranged by size:
3507 2039 429 194
BsrDI GCAATG_nn'
Cuts at: 65 1248 4664 4838 65
Size: 1183 3416 174 1396
Fragments arranged by size:
3416 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3435 4813 5000 1700
Size: 110 1625 1378 187 2869
Fragments arranged by size:
2869 1625 1378 187 110
BsrGI T'GTAC_A
Cuts at: 96 2818 96
Size: 2722 3447
BssHII G'CGCG_C
Cuts at: 1925 2098 1925
Size: 173 5996
BssSI C'ACGA_G
Cuts at: 4229 5241 4229
Size: 1012 5157
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6169
BstBI TT'CG_AA
Cuts at: 4973 4973
Size: 6169
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6169
BstYI r'GATC_y
Cuts at: 1972 3055 3203 3499 3866 4251 4268 5720 5806
Size: 1083 148 296 367 385 17 1452 86
Cuts at: 5806 5818 6164 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1083 385 367 346 296 148
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3417 3417
Size: 6169
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4678
Fragments arranged by size:
4678 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5430
Fragments arranged by size:
5430 683 56
BtsI GCAGTG_nn'
Cuts at: 2764 3685 4479 4499 2764
Size: 921 794 20 4434
Fragments arranged by size:
4434 921 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6169
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6134
DraI TTT'AAA
Cuts at: 2331 3473 2331
Size: 1142 5027
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5176 809
Size: 507 3860 1802
Fragments arranged by size:
3860 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3122 3616 4505
Size: 54 1425 50 275 18 1292 494 889
Cuts at: 4505 6134 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1292 889 494 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3616 1537
Size: 275 1804 4090
Fragments arranged by size:
4090 1804 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2119 2722 4099 1353
Size: 766 603 1377 3423
Fragments arranged by size:
3423 1377 766 603
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2027 4756 5130 5276 387
Size: 1064 138 438 2729 374 146 1280
Fragments arranged by size:
2729 1280 1064 438 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2313 2407 2886 3282 4232 4829 5616
Size: 393 681 94 479 396 950 597 787
Cuts at: 5616 5884 1239
Size: 268 1524
Fragments arranged by size:
1524 950 787 681 597 479 396 393
268 94
EcoNI CCTnn'n_nnAGG
Cuts at: 2601 3420 2601
Size: 819 5350
EcoRI G'AATT_C
Cuts at: 3478 3856 3478
Size: 378 5791
EcoRV GAT'ATC
Cuts at: 2499 2499
Size: 6169
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2576 2576
Size: 6169
FspI TGC'GCA
Cuts at: 4675 4675
Size: 6169
HaeII r_GCGC'y
Cuts at: 1678 5316 1678
Size: 3638 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2292 2324 3188 3220 3400
Size: 32 135 32 720 32 864 32 180
Cuts at: 3400 3432 4810 4842 4884 4916 1373
Size: 32 1378 32 42 32 2626
Fragments arranged by size:
2626 1378 864 720 180 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3513 3759 669
Size: 988 281 1575 246 3079
Fragments arranged by size:
3079 1575 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6169
HpaI GTT'AAC
Cuts at: 3759 3759
Size: 6169
KpnI G_GTAC'C
Cuts at: 3494 3494
Size: 6169
MfeI C'AATT_G
Cuts at: 3768 3768
Size: 6169
MluI A'CGCG_T
Cuts at: 6045 6045
Size: 6169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2436 2729 3142 5283 5467 1305
Size: 1131 293 413 2141 184 2007
Fragments arranged by size:
2141 2007 1131 413 293 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3124 10
Size: 54 1425 343 1292 3055
Fragments arranged by size:
3055 1425 1292 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4127 4486 4645 518
Size: 648 2961 359 159 2042
Fragments arranged by size:
2961 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2079 3585 4262 5410 5655 1606
Size: 473 1506 677 1148 245 2120
Fragments arranged by size:
2120 1506 1148 677 473 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4678
Fragments arranged by size:
4678 939 552
NdeI CA'TA_TG
Cuts at: 387 2476 387
Size: 2089 4080
NheI G'CTAG_C
Cuts at: 1052 2380 1052
Size: 1328 4841
NotI GC'GGCC_GC
Cuts at: 3616 3616
Size: 6169
NspI r_CATG'y
Cuts at: 1132 1229 3527 5072 1132
Size: 97 2298 1545 2229
Fragments arranged by size:
2298 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6169
PciI A'CATG_T
Cuts at: 5068 5068
Size: 6169
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2291 1108
Size: 1183 4986
PfoI T'CCnGG_A
Cuts at: 2148 2148
Size: 6169
PmeI GTTT'AAAC
Cuts at: 3473 3473
Size: 6169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4238 4270 5751 5783 4238
Size: 32 1481 32 4624
Fragments arranged by size:
4624 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6169
PsiI TTA'TAA
Cuts at: 2193 3739 2193
Size: 1546 4623
PstI C_TGCA'G
Cuts at: 830 2123 3521 830
Size: 1293 1398 3478
Fragments arranged by size:
3478 1398 1293
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6169
SalI G'TCGA_C
Cuts at: 1936 3511 1936
Size: 1575 4594
SapI GCTCTTCn'nnn_
Cuts at: 2119 2119
Size: 6169
SbfI CC_TGCA'GG
Cuts at: 3521 3521
Size: 6169
ScaI AGT'ACT
Cuts at: 4417 6129 4417
Size: 1712 4457
SfcI C'TryA_G
Cuts at: 826 1045 2119 3517 4652 5333 5524 826
Size: 219 1074 1398 1135 681 191 1471
Fragments arranged by size:
1471 1398 1135 1074 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6169
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6169
SmaI CCC'GGG
Cuts at: 1872 2038 2056 2086 3496 1872
Size: 166 18 30 1410 4545
Fragments arranged by size:
4545 1410 166 30 18
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2265 2683 2719 4273 5174 5436
Size: 197 926 322 418 36 1554 901 262
Cuts at: 5436 820
Size: 1553
Fragments arranged by size:
1554 1553 926 901 418 322 262 197
36
SnaBI TAC'GTA
Cuts at: 493 3960 493
Size: 3467 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6169
SphI G_CATG'C
Cuts at: 1132 3527 1132
Size: 2395 3774
SspI AAT'ATT
Cuts at: 5 52 4093 5
Size: 47 4041 2081
Fragments arranged by size:
4041 2081 47
StuI AGG'CCT
Cuts at: 1517 3286 1517
Size: 1769 4400
StyI C'CwwG_G
Cuts at: 513 1065 2004 2580 3608 513
Size: 552 939 576 1028 3074
Fragments arranged by size:
3074 1028 939 576 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4196 4213 4366 4551 4996 325
Size: 1255 2616 17 153 185 445 1498
Fragments arranged by size:
2616 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2657 2818 4415
Size: 274 80 33 51 1443 680 161 1597
Cuts at: 4415 6127 96
Size: 1712 138
Fragments arranged by size:
1712 1597 1443 680 274 161 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2743 2775 6129 6161 2743
Size: 32 3354 32 2751
Fragments arranged by size:
3354 2751 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6169
XbaI T'CTAG_A
Cuts at: 3505 3505
Size: 6169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3244 3244
Size: 6169
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6169
XmaI C'CCGG_G
Cuts at: 1870 2036 2054 2084 3494 1870
Size: 166 18 30 1410 4545
Fragments arranged by size:
4545 1410 166 30 18
XmnI GAAnn'nnTTC
Cuts at: 2394 4298 2394
Size: 1904 4265
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3977 276
Size: 53 83 186 936 2443 2468
Fragments arranged by size:
2468 2443 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI