Restriction map for
pFN21AB5645
(Circular) (Six-base) MAPSORT of: pFN21AB5645.seq Check: 6833 from: 1 to: 8215
pFN21AB5645 8215 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5238 5238
Size: 8215
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2547 6025 278
Size: 53 83 186 936 1011 3478 2468
Fragments arranged by size:
3478 2468 1011 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4452 5443 5558 1937
Size: 2515 991 115 4594
Fragments arranged by size:
4594 2515 991 115
Acc65I G'GTAC_C
Cuts at: 5536 5536
Size: 8215
AclI AA'CG_TT
Cuts at: 1563 6342 6715 1563
Size: 4779 373 3063
Fragments arranged by size:
4779 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2338 2931 5011 6278 7662 7930 1632
Size: 706 593 2080 1267 1384 268 1917
Fragments arranged by size:
2080 1917 1384 1267 706 593 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8018
AflIII A'CryG_T
Cuts at: 1203 3353 7114 8091 1203
Size: 2150 3761 977 1327
Fragments arranged by size:
3761 2150 1327 977
AgeI A'CCGG_T
Cuts at: 3822 7046 3822
Size: 3224 4991
AhdI GACnn_n'nnGTC
Cuts at: 2027 6944 2027
Size: 4917 3298
AleI CACnn'nnGTG
Cuts at: 1166 2873 3825 1166
Size: 1707 952 5556
Fragments arranged by size:
5556 1707 952
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3803 4757 1184
Size: 2619 954 4642
Fragments arranged by size:
4642 2619 954
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3160 3192 3160
Size: 32 8183
AlwNI CAG_nnn'CTG
Cuts at: 2075 3095 5219 5958 7530 2075
Size: 1020 2124 739 1572 2760
Fragments arranged by size:
2760 2124 1572 1020 739
ApaI G_GGCC'C
Cuts at: 2159 2500 2159
Size: 341 7874
ApaLI G'TGCA_C
Cuts at: 3760 6272 7428 3760
Size: 2512 1156 4547
Fragments arranged by size:
4547 2512 1156
ApoI r'AATT_y
Cuts at: 1493 3198 4242 5524 5750 5902 8011 1493
Size: 1705 1044 1282 226 152 2109 1697
Fragments arranged by size:
2109 1705 1697 1282 1044 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6769 160
Size: 872 5737 1606
Fragments arranged by size:
5737 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8215
AvaI C'yCGr_G
Cuts at: 1870 1943 2304 5179 5540 1870
Size: 73 361 2875 361 4545
Fragments arranged by size:
4545 2875 361 361 73
BamHI G'GATC_C
Cuts at: 3378 5545 5912 3378
Size: 2167 367 5681
Fragments arranged by size:
5681 2167 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2054 2139 2381 5033 5536
Size: 325 217 627 267 85 242 2652 503
Cuts at: 5536 6991 618
Size: 1455 1842
Fragments arranged by size:
2652 1842 1455 627 503 325 267 242
217 85
BanII G_rGCy'C
Cuts at: 1391 2159 2500 3251 5170 5534 1391
Size: 768 341 751 1919 364 4072
Fragments arranged by size:
4072 1919 768 751 364 341
BbeI G_GCGC'C
Cuts at: 2143 2143
Size: 8215
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2306 2344 2496 2554 2682 4191 5452
Size: 971 407 38 152 58 128 1509 1261
Cuts at: 5452 928
Size: 3691
Fragments arranged by size:
3691 1509 1261 971 407 152 128 58
38
BbvCI CC'TCA_GC
Cuts at: 2393 2939 2393
Size: 546 7669
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5051 5085 5276 5310 6406 6440 1903
Size: 34 3114 34 191 34 1096 34 3678
Fragments arranged by size:
3678 3114 1096 191 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4430 5168 6107 7317 4430
Size: 738 939 1210 5328
Fragments arranged by size:
5328 1210 939 738
BclI T'GATC_A
Cuts at: 1548 3369 1548
Size: 1821 6394
BfrBI ATG'CAT
Cuts at: 2044 2044
Size: 8215
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4649 6826 136
Size: 107 122 71 631 605 2977 2177 1525
Fragments arranged by size:
2977 2177 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3621 8210 3621
Size: 4589 3626
BlpI GC'TnA_GC
Cuts at: 5632 5632
Size: 8215
Bme1580I G_kGCm'C
Cuts at: 1790 2159 2312 2384 2500 3764 4638 4746 6276
Size: 369 153 72 116 1264 874 108 1530
Cuts at: 6276 7432 1790
Size: 1156 2573
Fragments arranged by size:
2573 1530 1264 1156 874 369 153 116
108 72
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8215
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3571 4095 4942
Size: 416 53 83 399 24 2154 524 847
Cuts at: 4942 5131 5155 5946 6904 442
Size: 189 24 791 958 1753
Fragments arranged by size:
2154 1753 958 847 791 524 416 399
189 83 53 24 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8215
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2964 3187 3268 2964
Size: 223 81 7911
Fragments arranged by size:
7911 223 81
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2345 2686 3337 6875 1239
Size: 1106 341 651 3538 2579
Fragments arranged by size:
3538 2579 1106 651 341
Bpu10I CC'TnA_GC
Cuts at: 2393 2939 5169 2393
Size: 546 2230 5439
Fragments arranged by size:
5439 2230 546
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5451 6340 7205 7503 5451
Size: 889 865 298 6163
Fragments arranged by size:
6163 889 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2565 3156 3437 4311 4859 6878 882
Size: 617 1066 591 281 874 548 2019 2219
Fragments arranged by size:
2219 2019 1066 874 617 591 548 281
BsaAI yAC'GTr
Cuts at: 493 1204 2422 6006 493
Size: 711 1218 3584 2702
Fragments arranged by size:
3584 2702 1218 711
BsaBI GATnn'nnATC
Cuts at: 8209 8209
Size: 8215
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2028 2140 2544 2613
Size: 53 83 186 936 495 112 404 69
Cuts at: 2613 3465 4389 5335 6022 6404 7039 275
Size: 852 924 946 687 382 635 1451
Fragments arranged by size:
1451 946 936 924 852 687 635 495
404 382 186 112 83 69 53
BsaWI w'CCGG_w
Cuts at: 3822 4528 6648 7046 7320 7467 7878 3822
Size: 706 2120 398 274 147 411 4159
Fragments arranged by size:
4159 2120 706 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3522 3552 1178
Size: 30 165 30 2119 30 5841
Fragments arranged by size:
5841 2119 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2711 3241 3546 1186
Size: 1525 530 305 5855
Fragments arranged by size:
5855 1525 530 305
BseYI C'CCAG_C
Cuts at: 2173 2499 3125 3398 3486 4282 7418 7914 2173
Size: 326 626 273 88 796 3136 496 2474
Fragments arranged by size:
3136 2474 796 626 496 326 273 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2504 2995 3937 1163
Size: 573 768 491 942 5441
Fragments arranged by size:
5441 942 768 573 491
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3214 5665 6426 7454 1540
Size: 275 186 1213 2451 761 1028 2301
Fragments arranged by size:
2451 2301 1213 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3764 3822 5229 6276 6361 7432 3764
Size: 58 1407 1047 85 1071 4547
Fragments arranged by size:
4547 1407 1071 1047 85 58
BsmI GAATG_Cn'
Cuts at: 2064 2536 3110 4710 5726 5819 2064
Size: 472 574 1600 1016 93 4460
Fragments arranged by size:
4460 1600 1016 574 472 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3560 4380 4407 5342 3560
Size: 820 27 935 6433
Fragments arranged by size:
6433 935 820 27
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2159 2312 2384 2500 3251 3764 3822
Size: 399 369 153 72 116 751 513 58
Cuts at: 3822 4638 4746 5170 5229 5534 6276 6361 7432
Size: 816 108 424 59 305 742 85 1071
Cuts at: 7432 1391
Size: 2174
Fragments arranged by size:
2174 1071 816 751 742 513 424 399
369 305 153 116 108 85 72 59
58
BspEI T'CCGG_A
Cuts at: 4528 7878 4528
Size: 3350 4865
BspHI T'CATG_A
Cuts at: 6104 7834 6104
Size: 1730 6485
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3278 5238 5570 844
Size: 2434 1960 332 3489
Fragments arranged by size:
3489 2434 1960 332
BsrBI CCG'CTC
Cuts at: 1394 1823 2329 3216 6102 1394
Size: 429 506 887 2886 3507
Fragments arranged by size:
3507 2886 887 506 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3199 4063 6710 6884 65
Size: 1183 1951 864 2647 174 1396
Fragments arranged by size:
2647 1951 1396 1183 864 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2453 3677 3822 3878 6859 7046 1700
Size: 110 643 1224 145 56 2981 187 2869
Fragments arranged by size:
2981 2869 1224 643 187 145 110 56
BsrGI T'GTAC_A
Cuts at: 96 2505 96
Size: 2409 5806
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8215
BssSI C'ACGA_G
Cuts at: 4739 5161 6275 7287 4739
Size: 422 1114 1012 5667
Fragments arranged by size:
5667 1114 1012 422
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3594 3759 4068 794
Size: 2800 165 309 4941
Fragments arranged by size:
4941 2800 309 165
BstBI TT'CG_AA
Cuts at: 7019 7019
Size: 8215
BstEII G'GTnAC_C
Cuts at: 1184 3498 1184
Size: 2314 5901
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2575 2011
Size: 564 7651
BstYI r'GATC_y
Cuts at: 1972 3378 3621 5509 5545 5912 6297 6314 7766
Size: 1406 243 1888 36 367 385 17 1452
Cuts at: 7766 7852 7864 8210 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1888 1452 1406 385 367 346 243
86 36 17 12
Bsu36I CC'TnA_GG
Cuts at: 2285 2684 2285
Size: 399 7816
BtgI C'CryG_G
Cuts at: 513 1065 2004 3155 3332 3503 3915 513
Size: 552 939 1151 177 171 412 4813
Fragments arranged by size:
4813 1151 939 552 412 177 171
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2569 2584 4788 487
Size: 683 56 1343 15 2204 3914
Fragments arranged by size:
3914 2204 1343 683 56 15
BtsI GCAGTG_nn'
Cuts at: 4138 5731 6525 6545 4138
Size: 1593 794 20 5808
Fragments arranged by size:
5808 1593 794 20
ClaI AT'CG_AT
Cuts at: 1554 4830 1554
Size: 3276 4939
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8180
DraI TTT'AAA
Cuts at: 3301 5519 3301
Size: 2218 5997
DraIII CAC_nnn'GTG
Cuts at: 2425 3018 5283 2425
Size: 593 2265 5357
Fragments arranged by size:
5357 2265 593
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7222 809
Size: 507 5906 1802
Fragments arranged by size:
5906 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2344 2455 2577
Size: 54 1425 50 275 18 514 111 122
Cuts at: 2577 2782 2928 3211 3895 3918 4122 4600 5266
Size: 205 146 283 684 23 204 478 666
Cuts at: 5266 5662 6551 8180 8
Size: 396 889 1629 43
Fragments arranged by size:
1629 1425 889 684 666 514 478 396
283 275 205 204 146 122 111 54
50 43 23 18
EagI C'GGCC_G
Cuts at: 1537 1812 3211 5662 1537
Size: 275 1399 2451 4090
Fragments arranged by size:
4090 2451 1399 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2459 2907 6145 1353
Size: 1106 448 3238 3423
Fragments arranged by size:
3423 3238 1106 448
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2094 4865 6802 7176 7322 387
Size: 1064 138 505 2771 1937 374 146 1280
Fragments arranged by size:
2771 1937 1280 1064 505 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2338 2345 2686 2931 3337 5011 6278
Size: 393 706 7 341 245 406 1674 1267
Cuts at: 6278 6875 7662 7930 1239
Size: 597 787 268 1524
Fragments arranged by size:
1674 1524 1267 787 706 597 406 393
341 268 245 7
EcoO109I rG'GnC_Cy
Cuts at: 2155 2156 2496 2860 3491 3963 5216 5411 2155
Size: 1 340 364 631 472 1253 195 4959
Fragments arranged by size:
4959 1253 631 472 364 340 195 1
EcoRI G'AATT_C
Cuts at: 3198 5524 5902 3198
Size: 2326 378 5511
Fragments arranged by size:
5511 2326 378
FspI TGC'GCA
Cuts at: 2867 5276 6721 2867
Size: 2409 1445 4361
Fragments arranged by size:
4361 2409 1445
FspAI rTGC'GCAy
Cuts at: 5276 5276
Size: 8215
HaeII r_GCGC'y
Cuts at: 1678 2143 7362 1678
Size: 465 5219 2531
Fragments arranged by size:
5219 2531 465
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2013 2045 2583 2615 2819
Size: 32 135 32 441 32 538 32 204
Cuts at: 2819 2851 3134 3166 4404 4436 4532 4564 4879
Size: 32 283 32 1238 32 96 32 315
Cuts at: 4879 4911 5322 5354 6856 6888 6930 6962 1373
Size: 32 411 32 1502 32 42 32 2626
Fragments arranged by size:
2626 1502 1238 538 441 411 315 283
204 135 96 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4855 4975 5559 5805 669
Size: 988 281 2917 120 584 246 3079
Fragments arranged by size:
3079 2917 988 584 281 246 120
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8215
HpaI GTT'AAC
Cuts at: 5805 5805
Size: 8215
KasI G'GCGC_C
Cuts at: 2139 2139
Size: 8215
KpnI G_GTAC'C
Cuts at: 5540 5540
Size: 8215
MfeI C'AATT_G
Cuts at: 3645 4507 5814 3645
Size: 862 1307 6046
Fragments arranged by size:
6046 1307 862
MluI A'CGCG_T
Cuts at: 8091 8091
Size: 8215
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2520 4471 7329 7513 1305
Size: 1215 1951 2858 184 2007
Fragments arranged by size:
2858 2007 1951 1215 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2346 2579 2784 2930 4124
Size: 54 1425 343 514 233 205 146 1194
Cuts at: 4124 4602 5268 10
Size: 478 666 2957
Fragments arranged by size:
2957 1425 1194 666 514 478 343 233
205 146 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2049 2873 3825 4478 4695 4815 5369
Size: 648 883 824 952 653 217 120 554
Cuts at: 5369 6173 6532 6691 518
Size: 804 359 159 2042
Fragments arranged by size:
2042 952 883 824 804 653 648 554
359 217 159 120
MspA1I CmG'CkG
Cuts at: 1606 2503 3711 4029 4323 4537 5631 6308 7456
Size: 897 1208 318 294 214 1094 677 1148
Cuts at: 7456 7701 1606
Size: 245 2120
Fragments arranged by size:
2120 1208 1148 1094 897 677 318 294
245 214
NaeI GCC'GGC
Cuts at: 3679 3679
Size: 8215
NarI GG'CG_CC
Cuts at: 2140 2140
Size: 8215
NcoI C'CATG_G
Cuts at: 513 1065 2004 3155 3332 3503 513
Size: 552 939 1151 177 171 5225
Fragments arranged by size:
5225 1151 939 552 177 171
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8215
NgoMIV G'CCGG_C
Cuts at: 3677 3677
Size: 8215
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8215
NotI GC'GGCC_GC
Cuts at: 3211 5662 3211
Size: 2451 5764
NsiI A_TGCA'T
Cuts at: 2046 2046
Size: 8215
NspI r_CATG'y
Cuts at: 1132 1229 2048 3357 3914 5573 7118 1132
Size: 97 819 1309 557 1659 1545 2229
Fragments arranged by size:
2229 1659 1545 1309 819 557 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8215
PciI A'CATG_T
Cuts at: 3353 7114 3353
Size: 3761 4454
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2075 2236 3650 1108
Size: 967 161 1414 5673
Fragments arranged by size:
5673 1414 967 161
PfoI T'CCnGG_A
Cuts at: 2847 4984 2847
Size: 2137 6078
PmeI GTTT'AAAC
Cuts at: 5519 5519
Size: 8215
PmlI CAC'GTG
Cuts at: 2422 2422
Size: 8215
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6284 6316 7797 7829 6284
Size: 32 1481 32 6670
Fragments arranged by size:
6670 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3491 3963 5411 3491
Size: 472 1448 6295
Fragments arranged by size:
6295 1448 472
PshAI GACnn'nnGTC
Cuts at: 1613 4263 4332 4623 4839 5295 1613
Size: 2650 69 291 216 456 4533
Fragments arranged by size:
4533 2650 456 291 216 69
PsiI TTA'TAA
Cuts at: 5785 5785
Size: 8215
PspOMI G'GGCC_C
Cuts at: 2155 2496 2155
Size: 341 7874
PstI C_TGCA'G
Cuts at: 830 2643 2889 4402 4736 5567 830
Size: 1813 246 1513 334 831 3478
Fragments arranged by size:
3478 1813 1513 831 334 246
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8215
PvuII CAG'CTG
Cuts at: 2503 3711 4537 2503
Size: 1208 826 6181
Fragments arranged by size:
6181 1208 826
SalI G'TCGA_C
Cuts at: 1936 5557 1936
Size: 3621 4594
SanDI GG'GwC_CC
Cuts at: 3963 3963
Size: 8215
SapI GCTCTTCn'nnn_
Cuts at: 2459 2459
Size: 8215
SbfI CC_TGCA'GG
Cuts at: 5567 5567
Size: 8215
ScaI AGT'ACT
Cuts at: 6463 8175 6463
Size: 1712 6503
SexAI A'CCwGG_T
Cuts at: 2235 4110 2235
Size: 1875 6340
SfcI C'TryA_G
Cuts at: 826 1045 2639 2885 4009 4398 4732 4995 5326
Size: 219 1594 246 1124 389 334 263 331
Cuts at: 5326 5563 6698 7379 7570 826
Size: 237 1135 681 191 1471
Fragments arranged by size:
1594 1471 1135 1124 681 389 334 331
263 246 237 219 191
SfoI GGC'GCC
Cuts at: 2141 2141
Size: 8215
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8215
SgrAI Cr'CCGG_yG
Cuts at: 1700 3822 1700
Size: 2122 6093
SmaI CCC'GGG
Cuts at: 1872 2306 5542 1872
Size: 434 3236 4545
Fragments arranged by size:
4545 3236 434
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5466 6319 7220 7482 820
Size: 197 926 3523 853 901 262 1553
Fragments arranged by size:
3523 1553 926 901 853 262 197
SnaBI TAC'GTA
Cuts at: 493 6006 493
Size: 5513 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8215
SphI G_CATG'C
Cuts at: 1132 5573 1132
Size: 4441 3774
SspI AAT'ATT
Cuts at: 5 52 6139 5
Size: 47 6087 2081
Fragments arranged by size:
6087 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8215
StyI C'CwwG_G
Cuts at: 513 1065 2004 2407 3155 3332 3494 3503 4515
Size: 552 939 403 748 177 162 9 1012
Cuts at: 4515 5654 513
Size: 1139 3074
Fragments arranged by size:
3074 1139 1012 939 748 552 403 177
162 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3244 4883 5150 6242 6259 6412 6597
Size: 1255 1664 1639 267 1092 17 153 185
Cuts at: 6597 7042 325
Size: 445 1498
Fragments arranged by size:
1664 1639 1498 1255 1092 445 267 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2505 2976 3689
Size: 274 80 33 51 1443 528 471 713
Cuts at: 3689 6461 8173 96
Size: 2772 1712 138
Fragments arranged by size:
2772 1712 1443 713 528 471 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3409 3441 3706 3738 8175 8207 3409
Size: 32 265 32 4437 32 3417
Fragments arranged by size:
4437 3417 265 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8215
XbaI T'CTAG_A
Cuts at: 5551 5551
Size: 8215
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2845 3162 4108 4664 2845
Size: 317 946 556 6396
Fragments arranged by size:
6396 946 556 317
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8215
XmaI C'CCGG_G
Cuts at: 1870 2304 5540 1870
Size: 434 3236 4545
Fragments arranged by size:
4545 3236 434
XmnI GAAnn'nnTTC
Cuts at: 5315 6344 5315
Size: 1029 7186
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2545 6023 276
Size: 53 83 186 936 1011 3478 2468
Fragments arranged by size:
3478 2468 1011 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BsiWI BstZ17I EcoICRI EcoNI
EcoRV FalI FseI NruI PacI PspXI PsrI RsrII
SacI SacII SfiI SrfI SwaI