Restriction map for
pFN21AB5641
(Circular) (Six-base) MAPSORT of: pFN21AB5641.seq Check: 2362 from: 1 to: 7006
pFN21AB5641 7006 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2100 4023 4816 278
Size: 53 83 186 936 564 1923 793 2468
Fragments arranged by size:
2468 1923 936 793 564 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3825 4349 1937
Size: 1888 524 4594
Fragments arranged by size:
4594 1888 524
Acc65I G'GTAC_C
Cuts at: 4327 4327
Size: 7006
AclI AA'CG_TT
Cuts at: 1563 2813 5133 5506 1563
Size: 1250 2320 373 3063
Fragments arranged by size:
3063 2320 1250 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2697 5069 6453 6721 1632
Size: 1065 2372 1384 268 1917
Fragments arranged by size:
2372 1917 1384 1065 268
AfeI AGC'GCT
Cuts at: 3696 4061 3696
Size: 365 6641
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6809
AflIII A'CryG_T
Cuts at: 1203 3432 4104 5905 6882 1203
Size: 2229 672 1801 977 1327
Fragments arranged by size:
2229 1801 1327 977 672
AgeI A'CCGG_T
Cuts at: 5837 5837
Size: 7006
AhdI GACnn_n'nnGTC
Cuts at: 3901 5735 3901
Size: 1834 5172
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7006
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3509 1184
Size: 2325 4681
AlwNI CAG_nnn'CTG
Cuts at: 2287 2779 3146 4749 6321 2287
Size: 492 367 1603 1572 2972
Fragments arranged by size:
2972 1603 1572 492 367
ApaLI G'TGCA_C
Cuts at: 2757 5063 6219 2757
Size: 2306 1156 3544
Fragments arranged by size:
3544 2306 1156
ApoI r'AATT_y
Cuts at: 1493 3588 4315 4541 4693 6802 1493
Size: 2095 727 226 152 2109 1697
Fragments arranged by size:
2109 2095 1697 727 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3111 5560 160
Size: 872 2079 2449 1606
Fragments arranged by size:
2449 2079 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7006
AvaI C'yCGr_G
Cuts at: 1870 1943 2458 4331 1870
Size: 73 515 1873 4545
Fragments arranged by size:
4545 1873 515 73
BamHI G'GATC_C
Cuts at: 2269 2769 4336 4703 2269
Size: 500 1567 367 4572
Fragments arranged by size:
4572 1567 500 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2782 4327 5782 618
Size: 325 217 627 995 1545 1455 1842
Fragments arranged by size:
1842 1545 1455 995 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2393 2442 4325 1391
Size: 1002 49 1883 4072
Fragments arranged by size:
4072 1883 1002 49
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2685 928
Size: 971 786 5249
Fragments arranged by size:
5249 971 786
BbvCI CC'TCA_GC
Cuts at: 2533 2533
Size: 7006
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4076 4110 5197 5231 1903
Size: 34 2139 34 1087 34 3678
Fragments arranged by size:
3678 2139 1087 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4898 6108 4898
Size: 1210 5796
BclI T'GATC_A
Cuts at: 1548 2872 1548
Size: 1324 5682
BfrBI ATG'CAT
Cuts at: 3200 3200
Size: 7006
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5617 136
Size: 107 122 71 631 605 3945 1525
Fragments arranged by size:
3945 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2521 3001 3654 7001 2521
Size: 480 653 3347 2526
Fragments arranged by size:
3347 2526 653 480
BlpI GC'TnA_GC
Cuts at: 3461 4423 3461
Size: 962 6044
Bme1580I G_kGCm'C
Cuts at: 1790 2761 5067 6223 1790
Size: 971 2306 1156 2573
Fragments arranged by size:
2573 2306 1156 971
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7006
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2659 3156 4737
Size: 416 53 83 399 24 1242 497 1581
Cuts at: 4737 5695 442
Size: 958 1753
Fragments arranged by size:
1753 1581 1242 958 497 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7006
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3676 3676
Size: 7006
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2990 3467 5666 1239
Size: 1751 477 2199 2579
Fragments arranged by size:
2579 2199 1751 477
Bpu10I CC'TnA_GC
Cuts at: 2387 2533 2387
Size: 146 6860
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5131 5996 6294 5131
Size: 865 298 5843
Fragments arranged by size:
5843 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5669 882
Size: 617 4170 2219
Fragments arranged by size:
4170 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3799 4303 4797 493
Size: 711 2595 504 494 2702
Fragments arranged by size:
2702 2595 711 504 494
BsaBI GATnn'nnATC
Cuts at: 7000 7000
Size: 7006
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2097 4020 4813 5195
Size: 53 83 186 936 564 1923 793 382
Cuts at: 5195 5830 275
Size: 635 1451
Fragments arranged by size:
1923 1451 936 793 635 564 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2766 5439 5837 6111 6258 6669 2766
Size: 2673 398 274 147 411 3103
Fragments arranged by size:
3103 2673 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3031 3061 1178
Size: 30 165 30 1628 30 5123
Fragments arranged by size:
5123 1628 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2810 1186
Size: 1624 5382
BseYI C'CCAG_C
Cuts at: 2116 2290 2481 2592 2837 2995 6209 6705 2116
Size: 174 191 111 245 158 3214 496 2417
Fragments arranged by size:
3214 2417 496 245 191 174 158 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2262 2747 3479 3821 4139 1163
Size: 573 526 485 732 342 318 4030
Fragments arranged by size:
4030 732 573 526 485 342 318
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4456 5217 6245 1540
Size: 275 186 2455 761 1028 2301
Fragments arranged by size:
2455 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2761 5067 5152 6223 2761
Size: 2306 85 1071 3544
Fragments arranged by size:
3544 2306 1071 85
BsmI GAATG_Cn'
Cuts at: 3691 4517 4610 3691
Size: 826 93 6087
Fragments arranged by size:
6087 826 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2992 2992
Size: 7006
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2393 2442 2761 4325 5067 5152 6223
Size: 399 603 49 319 1564 742 85 1071
Cuts at: 6223 1391
Size: 2174
Fragments arranged by size:
2174 1564 1071 742 603 399 319 85
49
BspEI T'CCGG_A
Cuts at: 6669 6669
Size: 7006
BspHI T'CATG_A
Cuts at: 4895 6625 4895
Size: 1730 5276
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4361 844
Size: 3517 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4893 1394
Size: 429 3070 3507
Fragments arranged by size:
3507 3070 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2928 3633 5501 5675 65
Size: 1183 1680 705 1868 174 1396
Fragments arranged by size:
1868 1680 1396 1183 705 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5650 5837 1700
Size: 110 3840 187 2869
Fragments arranged by size:
3840 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7006
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7006
BssSI C'ACGA_G
Cuts at: 3650 5066 6078 3650
Size: 1416 1012 4578
Fragments arranged by size:
4578 1416 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7006
BstBI TT'CG_AA
Cuts at: 2007 5810 2007
Size: 3803 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7006
BstXI CCAn_nnnn'nTGG
Cuts at: 3670 3697 3670
Size: 27 6979
BstYI r'GATC_y
Cuts at: 1972 2269 2521 2769 3001 3654 4336 4703 5088
Size: 297 252 248 232 653 682 367 385
Cuts at: 5088 5105 6557 6643 6655 7001 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 682 653 385 367 346 297
252 248 232 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2256 2569 3074 3477 513
Size: 552 1191 313 505 403 4042
Fragments arranged by size:
4042 1191 552 505 403 313
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2805 487
Size: 683 56 1579 4688
Fragments arranged by size:
4688 1579 683 56
BtsI GCAGTG_nn'
Cuts at: 2073 2394 2759 4522 5316 5336 2073
Size: 321 365 1763 794 20 3743
Fragments arranged by size:
3743 1763 794 365 321 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7006
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2605 2640 529
Size: 35 2041 35 4895
Fragments arranged by size:
4895 2041 35 35
DraI TTT'AAA
Cuts at: 3168 4310 3168
Size: 1142 5864
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2815 3833 6013 809
Size: 507 1499 1018 2180 1802
Fragments arranged by size:
2180 1802 1499 1018 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2076 2253 2683
Size: 54 1425 50 275 18 246 177 430
Cuts at: 2683 3547 4453 5342 6971 8
Size: 864 906 889 1629 43
Fragments arranged by size:
1629 1425 906 889 864 430 275 246
177 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4453 1537
Size: 275 2641 4090
Fragments arranged by size:
4090 2641 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2577 3312 3954 4213 4936 1353
Size: 1224 735 642 259 723 3423
Fragments arranged by size:
3423 1224 735 723 642 259
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5593 5967 6113 387
Size: 1064 138 4004 374 146 1280
Fragments arranged by size:
4004 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2697 2990 3467 5069 5666 6453 6721
Size: 393 1065 293 477 1602 597 787 268
Cuts at: 6721 1239
Size: 1524
Fragments arranged by size:
1602 1524 1065 787 597 477 393 293
268
EcoO109I rG'GnC_Cy
Cuts at: 2371 2937 3322 2371
Size: 566 385 6055
Fragments arranged by size:
6055 566 385
EcoRI G'AATT_C
Cuts at: 4315 4693 4315
Size: 378 6628
EcoRV GAT'ATC
Cuts at: 4142 4142
Size: 7006
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3044 3044
Size: 7006
FspI TGC'GCA
Cuts at: 5512 5512
Size: 7006
HaeII r_GCGC'y
Cuts at: 1678 3698 4063 6153 1678
Size: 2020 365 2090 2531
Fragments arranged by size:
2531 2090 2020 365
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2783 2815 2816 2848 3390
Size: 32 135 32 1211 32 1 32 542
Cuts at: 3390 3422 3887 3919 5647 5679 5721 5753 1373
Size: 32 465 32 1728 32 42 32 2626
Fragments arranged by size:
2626 1728 1211 542 465 135 42 32
32 32 32 32 32 32 32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 2056 3151 3355 4350 4596 669
Size: 988 281 118 1095 204 995 246 3079
Fragments arranged by size:
3079 1095 995 988 281 246 204 118
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7006
HpaI GTT'AAC
Cuts at: 3151 4596 3151
Size: 1445 5561
KpnI G_GTAC'C
Cuts at: 4331 4331
Size: 7006
MfeI C'AATT_G
Cuts at: 4605 4605
Size: 7006
MluI A'CGCG_T
Cuts at: 6882 6882
Size: 7006
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3788 3817 3909 6120 6304 1305
Size: 2483 29 92 2211 184 2007
Fragments arranged by size:
2483 2211 2007 184 92 29
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2078 10
Size: 54 1425 343 246 4938
Fragments arranged by size:
4938 1425 343 246 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3231 3521 3917 4292 4964 5323 5482
Size: 648 2065 290 396 375 672 359 159
Cuts at: 5482 518
Size: 2042
Fragments arranged by size:
2065 2042 672 648 396 375 359 290
159
MspA1I CmG'CkG
Cuts at: 1606 2042 2085 2276 2805 4422 5099 6247 6492
Size: 436 43 191 529 1617 677 1148 245
Cuts at: 6492 1606
Size: 2120
Fragments arranged by size:
2120 1617 1148 677 529 436 245 191
43
NcoI C'CATG_G
Cuts at: 513 1065 2256 2569 3074 3477 513
Size: 552 1191 313 505 403 4042
Fragments arranged by size:
4042 1191 552 505 403 313
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7006
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7006
NotI GC'GGCC_GC
Cuts at: 4453 4453
Size: 7006
NsiI A_TGCA'T
Cuts at: 3202 3202
Size: 7006
NspI r_CATG'y
Cuts at: 1132 1229 3140 3436 3460 3817 4135 4364 5909
Size: 97 1911 296 24 357 318 229 1545
Cuts at: 5909 1132
Size: 2229
Fragments arranged by size:
2229 1911 1545 357 318 296 229 97
24
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7006
PciI A'CATG_T
Cuts at: 3432 5905 3432
Size: 2473 4533
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2779 1108
Size: 1671 5335
PfoI T'CCnGG_A
Cuts at: 3444 3444
Size: 7006
PmeI GTTT'AAAC
Cuts at: 4310 4310
Size: 7006
PmlI CAC'GTG
Cuts at: 3799 3799
Size: 7006
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5075 5107 6588 6620 5075
Size: 32 1481 32 5461
Fragments arranged by size:
5461 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2371 2937 3322 2371
Size: 566 385 6055
Fragments arranged by size:
6055 566 385
PshAI GACnn'nnGTC
Cuts at: 1613 2665 1613
Size: 1052 5954
PsiI TTA'TAA
Cuts at: 4576 4576
Size: 7006
PspXI vC'TCGA_Gb
Cuts at: 2458 2458
Size: 7006
PstI C_TGCA'G
Cuts at: 830 2356 2401 2649 3707 4358 830
Size: 1526 45 248 1058 651 3478
Fragments arranged by size:
3478 1526 1058 651 248 45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7006
PvuII CAG'CTG
Cuts at: 2042 2276 2805 2042
Size: 234 529 6243
Fragments arranged by size:
6243 529 234
SalI G'TCGA_C
Cuts at: 1936 4348 1936
Size: 2412 4594
SbfI CC_TGCA'GG
Cuts at: 4358 4358
Size: 7006
ScaI AGT'ACT
Cuts at: 2034 3806 5254 6966 2034
Size: 1772 1448 1712 2074
Fragments arranged by size:
2074 1772 1712 1448
SfcI C'TryA_G
Cuts at: 826 1045 2091 2352 2397 2645 3703 3822 4354
Size: 219 1046 261 45 248 1058 119 532
Cuts at: 4354 5489 6170 6361 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1058 1046 681 532 261 248
219 191 119 45
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7006
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7006
SmaI CCC'GGG
Cuts at: 1872 4333 1872
Size: 2461 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2458 5110 6011 6273 820
Size: 197 926 515 2652 901 262 1553
Fragments arranged by size:
2652 1553 926 901 515 262 197
SnaBI TAC'GTA
Cuts at: 493 4303 4797 493
Size: 3810 494 2702
Fragments arranged by size:
3810 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7006
SphI G_CATG'C
Cuts at: 1132 3140 4364 1132
Size: 2008 1224 3774
Fragments arranged by size:
3774 2008 1224
SspI AAT'ATT
Cuts at: 5 52 4930 5
Size: 47 4878 2081
Fragments arranged by size:
4878 2081 47
StuI AGG'CCT
Cuts at: 1517 2612 1517
Size: 1095 5911
StyI C'CwwG_G
Cuts at: 513 1065 2061 2256 2557 2569 3074 3477 4445
Size: 552 996 195 301 12 505 403 968
Cuts at: 4445 513
Size: 3074
Fragments arranged by size:
3074 996 968 552 505 403 301 195
12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2460 2851 3995 5033 5050 5203 5388
Size: 1255 880 391 1144 1038 17 153 185
Cuts at: 5388 5833 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1144 1038 880 445 391 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2032 3804 5252
Size: 274 80 33 51 1443 55 1772 1448
Cuts at: 5252 6964 96
Size: 1712 138
Fragments arranged by size:
1772 1712 1448 1443 274 138 80 55
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6966 6998 6966
Size: 32 6974
Tth111I GACn'n_nGTC
Cuts at: 1316 3404 1316
Size: 2088 4918
XbaI T'CTAG_A
Cuts at: 4342 4342
Size: 7006
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2314 2314
Size: 7006
XhoI C'TCGA_G
Cuts at: 1943 2458 1943
Size: 515 6491
XmaI C'CCGG_G
Cuts at: 1870 4331 1870
Size: 2461 4545
XmnI GAAnn'nnTTC
Cuts at: 2034 5135 2034
Size: 3101 3905
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2098 4021 4814 276
Size: 53 83 186 936 564 1923 793 2468
Fragments arranged by size:
2468 1923 936 793 564 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PspOMI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI