Restriction map for
pFN21AB5640
(Circular) (Six-base) MAPSORT of: pFN21AB5640.seq Check: 3642 from: 1 to: 6241
pFN21AB5640 6241 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4051 278
Size: 53 83 186 936 2515 2468
Fragments arranged by size:
2515 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2982 3584 1937
Size: 1045 602 4594
Fragments arranged by size:
4594 1045 602
Acc65I G'GTAC_C
Cuts at: 3562 3562
Size: 6241
AclI AA'CG_TT
Cuts at: 1563 4368 4741 1563
Size: 2805 373 3063
Fragments arranged by size:
3063 2805 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2633 2846 3284 3380 4304 5688 5956 1632
Size: 1001 213 438 96 924 1384 268 1917
Fragments arranged by size:
1917 1384 1001 924 438 268 213 96
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6044
AflIII A'CryG_T
Cuts at: 1203 2156 5140 6117 1203
Size: 953 2984 977 1327
Fragments arranged by size:
2984 1327 977 953
AgeI A'CCGG_T
Cuts at: 5072 5072
Size: 6241
AhdI GACnn_n'nnGTC
Cuts at: 4970 4970
Size: 6241
AleI CACnn'nnGTG
Cuts at: 1166 3500 1166
Size: 2334 3907
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6241
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2078 2110 2078
Size: 32 6209
AlwNI CAG_nnn'CTG
Cuts at: 3984 5556 3984
Size: 1572 4669
ApaI G_GGCC'C
Cuts at: 2718 2718
Size: 6241
ApaLI G'TGCA_C
Cuts at: 4298 5454 4298
Size: 1156 5085
ApoI r'AATT_y
Cuts at: 1493 3550 3776 3928 6037 1493
Size: 2057 226 152 2109 1697
Fragments arranged by size:
2109 2057 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4795 160
Size: 872 3763 1606
Fragments arranged by size:
3763 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6241
AvaI C'yCGr_G
Cuts at: 1870 1943 2172 3566 1870
Size: 73 229 1394 4545
Fragments arranged by size:
4545 1394 229 73
BamHI G'GATC_C
Cuts at: 3571 3938 3571
Size: 367 5874
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2268 2578 3562 5017 618
Size: 325 217 627 481 310 984 1455 1842
Fragments arranged by size:
1842 1455 984 627 481 325 310 217
BanII G_rGCy'C
Cuts at: 1391 2261 2514 2718 3155 3177 3560 1391
Size: 870 253 204 437 22 383 4072
Fragments arranged by size:
4072 870 437 383 253 204 22
BbeI G_GCGC'C
Cuts at: 2272 2272
Size: 6241
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3171 3187 928
Size: 971 1272 16 3982
Fragments arranged by size:
3982 1272 971 16
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4432 4466 1903
Size: 34 2495 34 3678
Fragments arranged by size:
3678 2495 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2359 4133 5343 2359
Size: 1774 1210 3257
Fragments arranged by size:
3257 1774 1210
BclI T'GATC_A
Cuts at: 1548 2661 3076 1548
Size: 1113 415 4713
Fragments arranged by size:
4713 1113 415
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2345 4852 136
Size: 107 122 71 631 605 673 2507 1525
Fragments arranged by size:
2507 1525 673 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6236 6236
Size: 6241
BlpI GC'TnA_GC
Cuts at: 3154 3658 3154
Size: 504 5737
Bme1580I G_kGCm'C
Cuts at: 1790 2718 2911 4302 5458 1790
Size: 928 193 1391 1156 2573
Fragments arranged by size:
2573 1391 1156 928 193
BmgBI CAC'GTC
Cuts at: 1318 2215 1318
Size: 897 5344
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3972 4930 442
Size: 416 53 83 399 24 2555 958 1753
Fragments arranged by size:
2555 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2105 2375 2426 2667 2861 2941 4901 1239
Size: 866 270 51 241 194 80 1960 2579
Fragments arranged by size:
2579 1960 866 270 241 194 80 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4366 5231 5529 4366
Size: 865 298 5078
Fragments arranged by size:
5078 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3030 4904 882
Size: 617 1531 1874 2219
Fragments arranged by size:
2219 1874 1531 617
BsaAI yAC'GTr
Cuts at: 493 1204 4032 493
Size: 711 2828 2702
Fragments arranged by size:
2828 2702 711
BsaBI GATnn'nnATC
Cuts at: 2108 6235 2108
Size: 4127 2114
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2269 4048 4430 5065
Size: 53 83 186 936 736 1779 382 635
Cuts at: 5065 275
Size: 1451
Fragments arranged by size:
1779 1451 936 736 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4674 5072 5346 5493 5904 4674
Size: 398 274 147 411 5011
Fragments arranged by size:
5011 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2078 2108 1178
Size: 30 165 30 675 30 5311
Fragments arranged by size:
5311 675 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2987 3248 1186
Size: 1801 261 4179
Fragments arranged by size:
4179 1801 261
BseYI C'CCAG_C
Cuts at: 2845 5444 5940 2845
Size: 2599 496 3146
Fragments arranged by size:
3146 2599 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2220 2288 1163
Size: 573 484 68 5116
Fragments arranged by size:
5116 573 484 68
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3691 4452 5480 1540
Size: 275 186 1690 761 1028 2301
Fragments arranged by size:
2301 1690 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4302 4387 5458 4302
Size: 85 1071 5085
Fragments arranged by size:
5085 1071 85
BsmI GAATG_Cn'
Cuts at: 3149 3752 3845 3149
Size: 603 93 5545
Fragments arranged by size:
5545 603 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2351 2351
Size: 6241
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2261 2514 2718 2911 3155 3177 3560
Size: 399 471 253 204 193 244 22 383
Cuts at: 3560 4302 4387 5458 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 471 399 383 253 244
204 193 85 22
BspEI T'CCGG_A
Cuts at: 5904 5904
Size: 6241
BspHI T'CATG_A
Cuts at: 4130 5860 4130
Size: 1730 4511
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2968 3596 844
Size: 2124 628 3489
Fragments arranged by size:
3489 2124 628
BsrBI CCG'CTC
Cuts at: 1394 1823 4128 1394
Size: 429 2305 3507
Fragments arranged by size:
3507 2305 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4736 4910 65
Size: 1183 3488 174 1396
Fragments arranged by size:
3488 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2056 2271 2771 4885 5072 1700
Size: 110 246 215 500 2114 187 2869
Fragments arranged by size:
2869 2114 500 246 215 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6241
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6241
BssSI C'ACGA_G
Cuts at: 4301 5313 4301
Size: 1012 5229
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2243 794
Size: 1449 4792
BstBI TT'CG_AA
Cuts at: 5045 5045
Size: 6241
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6241
BstYI r'GATC_y
Cuts at: 1972 3571 3938 4323 4340 5792 5878 5890 6236
Size: 1599 367 385 17 1452 86 12 346
Cuts at: 6236 1972
Size: 1977
Fragments arranged by size:
1977 1599 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2025 2264 2540 2604 3030 3159
Size: 552 939 21 239 276 64 426 129
Cuts at: 3159 513
Size: 3595
Fragments arranged by size:
3595 939 552 426 276 239 129 64
21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5502
Fragments arranged by size:
5502 683 56
BtsI GCAGTG_nn'
Cuts at: 2904 3757 4551 4571 2904
Size: 853 794 20 4574
Fragments arranged by size:
4574 853 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6241
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2767 2802 529
Size: 35 2203 35 3968
Fragments arranged by size:
3968 2203 35 35
DraI TTT'AAA
Cuts at: 3445 3545 3445
Size: 100 6141
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5248 809
Size: 507 3932 1802
Fragments arranged by size:
3932 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2346 3486
Size: 54 1425 50 275 18 177 339 1140
Cuts at: 3486 3688 4577 6206 8
Size: 202 889 1629 43
Fragments arranged by size:
1629 1425 1140 889 339 275 202 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3688 1537
Size: 275 1876 4090
Fragments arranged by size:
4090 1876 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3182 4171 1353
Size: 1829 989 3423
Fragments arranged by size:
3423 1829 989
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3016 4828 5202 5348 387
Size: 1064 138 1427 1812 374 146 1280
Fragments arranged by size:
1812 1427 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2375 2426 2633 2667 2846 2861
Size: 393 473 270 51 207 34 179 15
Cuts at: 2861 2941 3284 3380 4304 4901 5688 5956 1239
Size: 80 343 96 924 597 787 268 1524
Fragments arranged by size:
1524 924 787 597 473 393 343 270
268 207 179 96 80 51 34 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2251 2251
Size: 6241
EcoO109I rG'GnC_Cy
Cuts at: 3065 3065
Size: 6241
EcoRI G'AATT_C
Cuts at: 3550 3928 3550
Size: 378 5863
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2782 3193 2782
Size: 411 5830
FspI TGC'GCA
Cuts at: 4747 4747
Size: 6241
HaeII r_GCGC'y
Cuts at: 1678 2272 5388 1678
Size: 594 3116 2531
Fragments arranged by size:
3116 2531 594
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4882 4914 4956 4988 1373
Size: 32 135 32 3310 32 42 32 2626
Fragments arranged by size:
3310 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3585 3831 669
Size: 988 281 1647 246 3079
Fragments arranged by size:
3079 1647 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6241
HpaI GTT'AAC
Cuts at: 3831 3831
Size: 6241
KasI G'GCGC_C
Cuts at: 2268 2268
Size: 6241
KpnI G_GTAC'C
Cuts at: 3566 3566
Size: 6241
MfeI C'AATT_G
Cuts at: 3840 3840
Size: 6241
MluI A'CGCG_T
Cuts at: 6117 6117
Size: 6241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2767 5355 5539 1305
Size: 1462 2588 184 2007
Fragments arranged by size:
2588 2007 1462 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 4242
Fragments arranged by size:
4242 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3500 4199 4558 4717 518
Size: 648 2334 699 359 159 2042
Fragments arranged by size:
2334 2042 699 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2311 3657 4334 5482 5727 1606
Size: 705 1346 677 1148 245 2120
Fragments arranged by size:
2120 1346 1148 705 677 245
NaeI GCC'GGC
Cuts at: 2058 2273 2058
Size: 215 6026
NarI GG'CG_CC
Cuts at: 2269 2269
Size: 6241
NcoI C'CATG_G
Cuts at: 513 1065 2004 2264 2540 2604 3159 513
Size: 552 939 260 276 64 555 3595
Fragments arranged by size:
3595 939 555 552 276 260 64
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6241
NgoMIV G'CCGG_C
Cuts at: 2056 2271 2056
Size: 215 6026
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6241
NotI GC'GGCC_GC
Cuts at: 3688 3688
Size: 6241
NspI r_CATG'y
Cuts at: 1132 1229 2160 3220 3599 5144 1132
Size: 97 931 1060 379 1545 2229
Fragments arranged by size:
2229 1545 1060 931 379 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6241
PciI A'CATG_T
Cuts at: 2156 5140 2156
Size: 2984 3257
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3437 1108
Size: 2329 3912
PfoI T'CCnGG_A
Cuts at: 2683 2813 2683
Size: 130 6111
PmeI GTTT'AAAC
Cuts at: 3545 3545
Size: 6241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2078 2110 2660 2692 4310 4342 5823 5855 2078
Size: 32 550 32 1618 32 1481 32 2464
Fragments arranged by size:
2464 1618 1481 550 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6241
PsiI TTA'TAA
Cuts at: 3811 3811
Size: 6241
PspOMI G'GGCC_C
Cuts at: 2714 2714
Size: 6241
PstI C_TGCA'G
Cuts at: 830 3593 830
Size: 2763 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6241
PvuII CAG'CTG
Cuts at: 2311 2311
Size: 6241
SalI G'TCGA_C
Cuts at: 1936 3583 1936
Size: 1647 4594
SapI GCTCTTCn'nnn_
Cuts at: 3182 3182
Size: 6241
SbfI CC_TGCA'GG
Cuts at: 3593 3593
Size: 6241
ScaI AGT'ACT
Cuts at: 4489 6201 4489
Size: 1712 4529
SexAI A'CCwGG_T
Cuts at: 2319 2319
Size: 6241
SfcI C'TryA_G
Cuts at: 826 1045 3589 4724 5405 5596 826
Size: 219 2544 1135 681 191 1471
Fragments arranged by size:
2544 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2270 2270
Size: 6241
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6241
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6241
SmaI CCC'GGG
Cuts at: 1872 3568 1872
Size: 1696 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4345 5246 5508 820
Size: 197 926 2402 901 262 1553
Fragments arranged by size:
2402 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4032 493
Size: 3539 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6241
SphI G_CATG'C
Cuts at: 1132 3599 1132
Size: 2467 3774
SspI AAT'ATT
Cuts at: 5 52 4165 5
Size: 47 4113 2081
Fragments arranged by size:
4113 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6241
StyI C'CwwG_G
Cuts at: 513 1065 2004 2264 2343 2540 2604 2932 3159
Size: 552 939 260 79 197 64 328 227
Cuts at: 3159 3680 513
Size: 521 3074
Fragments arranged by size:
3074 939 552 521 328 260 227 197
79 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4268 4285 4438 4623 5068 325
Size: 1255 2688 17 153 185 445 1498
Fragments arranged by size:
2688 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4487 6199 96
Size: 274 80 33 51 1443 2510 1712 138
Fragments arranged by size:
2510 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6201 6233 6201
Size: 32 6209
Tth111I GACn'n_nGTC
Cuts at: 1316 2486 1316
Size: 1170 5071
XbaI T'CTAG_A
Cuts at: 3577 3577
Size: 6241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2080 2965 2080
Size: 885 5356
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6241
XmaI C'CCGG_G
Cuts at: 1870 3566 1870
Size: 1696 4545
XmnI GAAnn'nnTTC
Cuts at: 4370 4370
Size: 6241
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4049 276
Size: 53 83 186 936 2515 2468
Fragments arranged by size:
2515 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI
PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI