Restriction map for
pFN21AB5639
(Circular) (Six-base) MAPSORT of: pFN21AB5639.seq Check: 2126 from: 1 to: 6025
pFN21AB5639 6025 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3835 278
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3368 1937
Size: 1431 4594
Acc65I G'GTAC_C
Cuts at: 3346 3346
Size: 6025
AclI AA'CG_TT
Cuts at: 1563 4152 4525 1563
Size: 2589 373 3063
Fragments arranged by size:
3063 2589 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4088 5472 5740 1632
Size: 2456 1384 268 1917
Fragments arranged by size:
2456 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5828
AflIII A'CryG_T
Cuts at: 1203 2907 4924 5901 1203
Size: 1704 2017 977 1327
Fragments arranged by size:
2017 1704 1327 977
AgeI A'CCGG_T
Cuts at: 4856 4856
Size: 6025
AhdI GACnn_n'nnGTC
Cuts at: 4754 4754
Size: 6025
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6025
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6025
AlwNI CAG_nnn'CTG
Cuts at: 2954 3314 3768 5340 2954
Size: 360 454 1572 3639
Fragments arranged by size:
3639 1572 454 360
ApaI G_GGCC'C
Cuts at: 2047 2047
Size: 6025
ApaLI G'TGCA_C
Cuts at: 4082 5238 4082
Size: 1156 4869
ApoI r'AATT_y
Cuts at: 1493 3334 3560 3712 5821 1493
Size: 1841 226 152 2109 1697
Fragments arranged by size:
2109 1841 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4579 160
Size: 872 3547 1606
Fragments arranged by size:
3547 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
AvaI C'yCGr_G
Cuts at: 1870 1943 2918 3350 1870
Size: 73 975 432 4545
Fragments arranged by size:
4545 975 432 73
BamHI G'GATC_C
Cuts at: 3355 3722 3355
Size: 367 5658
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2429 3049 3346 4801 618
Size: 325 217 627 642 620 297 1455 1842
Fragments arranged by size:
1842 1455 642 627 620 325 297 217
BanII G_rGCy'C
Cuts at: 1391 2047 2100 3278 3344 1391
Size: 656 53 1178 66 4072
Fragments arranged by size:
4072 1178 656 66 53
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2277 928
Size: 971 378 4676
Fragments arranged by size:
4676 971 378
BbvCI CC'TCA_GC
Cuts at: 2805 3123 2805
Size: 318 5707
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3020 3054 4216 4250 1903
Size: 34 1083 34 1162 34 3678
Fragments arranged by size:
3678 1162 1083 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3917 5127 3917
Size: 1210 4815
BclI T'GATC_A
Cuts at: 1548 2169 1548
Size: 621 5404
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4636 136
Size: 107 122 71 631 605 2964 1525
Fragments arranged by size:
2964 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6020 6020
Size: 6025
BlpI GC'TnA_GC
Cuts at: 3442 3442
Size: 6025
Bme1580I G_kGCm'C
Cuts at: 1790 2047 3054 4086 5242 1790
Size: 257 1007 1032 1156 2573
Fragments arranged by size:
2573 1156 1032 1007 257
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6025
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3756 4714 442
Size: 416 53 83 399 24 2339 958 1753
Fragments arranged by size:
2339 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2542 2587 4685 1239
Size: 1303 45 2098 2579
Fragments arranged by size:
2579 2098 1303 45
Bpu10I CC'TnA_GC
Cuts at: 2749 2769 2805 2859 3123 2749
Size: 20 36 54 264 5651
Fragments arranged by size:
5651 264 54 36 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4150 5015 5313 4150
Size: 865 298 4862
Fragments arranged by size:
4862 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2155 2967 4688 882
Size: 617 656 812 1721 2219
Fragments arranged by size:
2219 1721 812 656 617
BsaAI yAC'GTr
Cuts at: 493 1204 3816 493
Size: 711 2612 2702
Fragments arranged by size:
2702 2612 711
BsaBI GATnn'nnATC
Cuts at: 6019 6019
Size: 6025
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3832 4214 4849 275
Size: 53 83 186 936 2299 382 635 1451
Fragments arranged by size:
2299 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4458 4856 5130 5277 5688 4458
Size: 398 274 147 411 4795
Fragments arranged by size:
4795 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2646 2676 1178
Size: 30 165 30 1243 30 4527
Fragments arranged by size:
4527 1243 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2603 2983 2986 1186
Size: 1417 380 3 4225
Fragments arranged by size:
4225 1417 380 3
BseYI C'CCAG_C
Cuts at: 2193 2578 3229 5228 5724 2193
Size: 385 651 1999 496 2494
Fragments arranged by size:
2494 1999 651 496 385
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2335 2374 1163
Size: 573 599 39 4814
Fragments arranged by size:
4814 599 573 39
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2034 3475 4236 5264 1540
Size: 275 186 33 1441 761 1028 2301
Fragments arranged by size:
2301 1441 1028 761 275 186 33
BsiHKAI G_wGCw'C
Cuts at: 2124 3278 4086 4171 5242 2124
Size: 1154 808 85 1071 2907
Fragments arranged by size:
2907 1154 1071 808 85
BsmI GAATG_Cn'
Cuts at: 3536 3629 3536
Size: 93 5932
BsmBI CGTCTCn'nnnn_
Cuts at: 2585 3218 2585
Size: 633 5392
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2047 2100 2124 3054 3278 3344 4086
Size: 399 257 53 24 930 224 66 742
Cuts at: 4086 4171 5242 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 930 742 399 257 224 85
66 53 24
BspEI T'CCGG_A
Cuts at: 5688 5688
Size: 6025
BspHI T'CATG_A
Cuts at: 3075 3914 5644 3075
Size: 839 1730 3456
Fragments arranged by size:
3456 1730 839
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3380 844
Size: 2536 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2204 3073 3912 1394
Size: 429 381 869 839 3507
Fragments arranged by size:
3507 869 839 429 381
BsrDI GCAATG_nn'
Cuts at: 65 1248 4520 4694 65
Size: 1183 3272 174 1396
Fragments arranged by size:
3272 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2944 4669 4856 1700
Size: 110 1134 1725 187 2869
Fragments arranged by size:
2869 1725 1134 187 110
BsrGI T'GTAC_A
Cuts at: 96 2513 3150 96
Size: 2417 637 2971
Fragments arranged by size:
2971 2417 637
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6025
BssSI C'ACGA_G
Cuts at: 4085 5097 4085
Size: 1012 5013
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2292 3314 794
Size: 1498 1022 3505
Fragments arranged by size:
3505 1498 1022
BstBI TT'CG_AA
Cuts at: 4829 4829
Size: 6025
BstEII G'GTnAC_C
Cuts at: 1184 2596 1184
Size: 1412 4613
BstXI CCAn_nnnn'nTGG
Cuts at: 2494 2494
Size: 6025
BstYI r'GATC_y
Cuts at: 1972 3355 3722 4107 4124 5576 5662 5674 6020
Size: 1383 367 385 17 1452 86 12 346
Cuts at: 6020 1972
Size: 1977
Fragments arranged by size:
1977 1452 1383 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2133 513
Size: 552 939 129 4405
Fragments arranged by size:
4405 939 552 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5286
Fragments arranged by size:
5286 683 56
BtsI GCAGTG_nn'
Cuts at: 3541 4335 4355 3541
Size: 794 20 5211
Fragments arranged by size:
5211 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6025
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5990
DraI TTT'AAA
Cuts at: 3329 3329
Size: 6025
DraIII CAC_nnn'GTG
Cuts at: 2358 2412 2481 2358
Size: 54 69 5902
Fragments arranged by size:
5902 69 54
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5032 809
Size: 507 3716 1802
Fragments arranged by size:
3716 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 3472 4361
Size: 54 1425 50 275 18 201 1441 889
Cuts at: 4361 5990 8
Size: 1629 43
Fragments arranged by size:
1629 1441 1425 889 275 201 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2031 3472 1537
Size: 275 219 1441 4090
Fragments arranged by size:
4090 1441 275 219
EarI CTCTTCn'nnn_
Cuts at: 1353 3955 1353
Size: 2602 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4612 4986 5132 387
Size: 1064 138 3023 374 146 1280
Fragments arranged by size:
3023 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3276 3276
Size: 6025
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2542 2587 4088 4685 5472 5740 1239
Size: 393 910 45 1501 597 787 268 1524
Fragments arranged by size:
1524 1501 910 787 597 393 268 45
EcoO109I rG'GnC_Cy
Cuts at: 2043 2066 2104 2764 2854 2043
Size: 23 38 660 90 5214
Fragments arranged by size:
5214 660 90 38 23
EcoRI G'AATT_C
Cuts at: 3334 3712 3334
Size: 378 5647
FspI TGC'GCA
Cuts at: 4531 4531
Size: 6025
HaeII r_GCGC'y
Cuts at: 1678 5172 1678
Size: 3494 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2247 2271 2273 2279 2303
Size: 32 135 32 675 24 2 6 24
Cuts at: 2303 2305 2481 2513 2771 2803 4666 4698 4740
Size: 2 176 32 258 32 1863 32 42
Cuts at: 4740 4772 1373
Size: 32 2626
Fragments arranged by size:
2626 1863 675 258 176 135 42 32
32 32 32 32 32 24 24 6
2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2905 3175 3369 3615 669
Size: 988 281 967 270 194 246 3079
Fragments arranged by size:
3079 988 967 281 270 246 194
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6025
HpaI GTT'AAC
Cuts at: 3615 3615
Size: 6025
KpnI G_GTAC'C
Cuts at: 3350 3350
Size: 6025
MfeI C'AATT_G
Cuts at: 3624 3624
Size: 6025
MluI A'CGCG_T
Cuts at: 5901 5901
Size: 6025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5139 5323 1305
Size: 3834 184 2007
Fragments arranged by size:
3834 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4203
Fragments arranged by size:
4203 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2325 3983 4342 4501 518
Size: 648 1159 1658 359 159 2042
Fragments arranged by size:
2042 1658 1159 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2135 2156 2582 3131 3441 4118 5266 5511
Size: 529 21 426 549 310 677 1148 245
Cuts at: 5511 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 549 529 426 310 245
21
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4534
Fragments arranged by size:
4534 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6025
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6025
NotI GC'GGCC_GC
Cuts at: 3472 3472
Size: 6025
NspI r_CATG'y
Cuts at: 1132 1229 2911 3383 4928 1132
Size: 97 1682 472 1545 2229
Fragments arranged by size:
2229 1682 1545 472 97
PasI CC'CwG_GG
Cuts at: 1348 2070 2609 1348
Size: 722 539 4764
Fragments arranged by size:
4764 722 539
PciI A'CATG_T
Cuts at: 2907 4924 2907
Size: 2017 4008
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6025
PfoI T'CCnGG_A
Cuts at: 2436 2877 2436
Size: 441 5584
PmeI GTTT'AAAC
Cuts at: 3329 3329
Size: 6025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4094 4126 5607 5639 4094
Size: 32 1481 32 4480
Fragments arranged by size:
4480 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2066 2104 2764 2854 2066
Size: 38 660 90 5237
Fragments arranged by size:
5237 660 90 38
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6025
PsiI TTA'TAA
Cuts at: 3595 3595
Size: 6025
PspOMI G'GGCC_C
Cuts at: 2043 2043
Size: 6025
PstI C_TGCA'G
Cuts at: 830 3377 830
Size: 2547 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6025
PvuII CAG'CTG
Cuts at: 2156 2582 2156
Size: 426 5599
SacI G_AGCT'C
Cuts at: 3278 3278
Size: 6025
SacII CC_GC'GG
Cuts at: 2136 2136
Size: 6025
SalI G'TCGA_C
Cuts at: 1936 3367 1936
Size: 1431 4594
SanDI GG'GwC_CC
Cuts at: 2104 2764 2104
Size: 660 5365
SbfI CC_TGCA'GG
Cuts at: 3377 3377
Size: 6025
ScaI AGT'ACT
Cuts at: 4273 5985 4273
Size: 1712 4313
SexAI A'CCwGG_T
Cuts at: 2354 2354
Size: 6025
SfcI C'TryA_G
Cuts at: 826 1045 2311 3373 4508 5189 5380 826
Size: 219 1266 1062 1135 681 191 1471
Fragments arranged by size:
1471 1266 1135 1062 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6025
SmaI CCC'GGG
Cuts at: 1872 2920 3352 1872
Size: 1048 432 4545
Fragments arranged by size:
4545 1048 432
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4129 5030 5292 820
Size: 197 926 2186 901 262 1553
Fragments arranged by size:
2186 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3816 493
Size: 3323 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6025
SphI G_CATG'C
Cuts at: 1132 3383 1132
Size: 2251 3774
SspI AAT'ATT
Cuts at: 5 52 3949 5
Size: 47 3897 2081
Fragments arranged by size:
3897 2081 47
StuI AGG'CCT
Cuts at: 1517 2092 1517
Size: 575 5450
StyI C'CwwG_G
Cuts at: 513 1065 2004 3219 3464 513
Size: 552 939 1215 245 3074
Fragments arranged by size:
3074 1215 939 552 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2189 2864 4052 4069 4222 4407 4852
Size: 1255 609 675 1188 17 153 185 445
Cuts at: 4852 325
Size: 1498
Fragments arranged by size:
1498 1255 1188 675 609 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2513 3150 4271
Size: 274 80 33 51 1443 536 637 1121
Cuts at: 4271 5983 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1121 637 536 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2467 2499 5985 6017 2467
Size: 32 3486 32 2475
Fragments arranged by size:
3486 2475 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2813 1316
Size: 1497 4528
XbaI T'CTAG_A
Cuts at: 3295 3361 3295
Size: 66 5959
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6025
XmaI C'CCGG_G
Cuts at: 1870 2918 3350 1870
Size: 1048 432 4545
Fragments arranged by size:
4545 1048 432
XmnI GAAnn'nnTTC
Cuts at: 4154 4154
Size: 6025
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3833 276
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SapI SfiI SfoI SrfI
SwaI XcmI