Restriction map for
pFN21AB5637
(Circular) (Six-base) MAPSORT of: pFN21AB5637.seq Check: 8371 from: 1 to: 7936
pFN21AB5637 7936 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2815 3217 2815
Size: 402 7534
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2212 5746 278
Size: 53 83 186 936 676 3534 2468
Fragments arranged by size:
3534 2468 936 676 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5279 1937
Size: 3342 4594
Acc65I G'GTAC_C
Cuts at: 3091 5257 3091
Size: 2166 5770
AclI AA'CG_TT
Cuts at: 1563 6063 6436 1563
Size: 4500 373 3063
Fragments arranged by size:
4500 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2859 3405 3498 4665 4981 5999 7383 7651
Size: 1227 546 93 1167 316 1018 1384 268
Cuts at: 7651 1632
Size: 1917
Fragments arranged by size:
1917 1384 1227 1167 1018 546 316 268
93
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7739
AflIII A'CryG_T
Cuts at: 1203 2465 6835 7812 1203
Size: 1262 4370 977 1327
Fragments arranged by size:
4370 1327 1262 977
AgeI A'CCGG_T
Cuts at: 6767 6767
Size: 7936
AhdI GACnn_n'nnGTC
Cuts at: 4372 6665 4372
Size: 2293 5643
AleI CACnn'nnGTG
Cuts at: 1166 2987 1166
Size: 1821 6115
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7936
AlwNI CAG_nnn'CTG
Cuts at: 2807 3239 5071 5679 7251 2807
Size: 432 1832 608 1572 3492
Fragments arranged by size:
3492 1832 1572 608 432
ApaI G_GGCC'C
Cuts at: 2276 2276
Size: 7936
ApaLI G'TGCA_C
Cuts at: 5993 7149 5993
Size: 1156 6780
ApoI r'AATT_y
Cuts at: 1493 4160 4187 4489 4645 4949 5021 5088 5245
Size: 2667 27 302 156 304 72 67 157
Cuts at: 5245 5471 5623 7732 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2667 2109 1697 304 302 226 157 156
152 72 67 27
AseI AT'TA_AT
Cuts at: 160 1032 3779 3792 5048 6490 160
Size: 872 2747 13 1256 1442 1606
Fragments arranged by size:
2747 1606 1442 1256 872 13
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7936
AvaI C'yCGr_G
Cuts at: 1870 1943 2050 3894 5261 1870
Size: 73 107 1844 1367 4545
Fragments arranged by size:
4545 1844 1367 107 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3083 3116 3083
Size: 33 7903
BamHI G'GATC_C
Cuts at: 3418 4742 5266 5633 3418
Size: 1324 524 367 5721
Fragments arranged by size:
5721 1324 524 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3091 3705 5257 6712 618
Size: 325 217 627 1304 614 1552 1455 1842
Fragments arranged by size:
1842 1552 1455 1304 627 614 325 217
BanII G_rGCy'C
Cuts at: 1391 2061 2276 3459 3666 5217 5255 1391
Size: 670 215 1183 207 1551 38 4072
Fragments arranged by size:
4072 1551 1183 670 215 207 38
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3278 3565 4313 4458 928
Size: 971 1379 287 748 145 4406
Fragments arranged by size:
4406 1379 971 748 287 145
BbvCI CC'TCA_GC
Cuts at: 3051 3051
Size: 7936
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6127 6161 1903
Size: 34 4190 34 3678
Fragments arranged by size:
4190 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2250 2824 5828 7038 2250
Size: 574 3004 1210 3148
Fragments arranged by size:
3148 3004 1210 574
BclI T'GATC_A
Cuts at: 1548 4060 1548
Size: 2512 5424
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3500 6547 136
Size: 107 122 71 631 605 1828 3047 1525
Fragments arranged by size:
3047 1828 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7931 7931
Size: 7936
BlpI GC'TnA_GC
Cuts at: 5353 5353
Size: 7936
Bme1580I G_kGCm'C
Cuts at: 1790 2276 5997 7153 1790
Size: 486 3721 1156 2573
Fragments arranged by size:
3721 2573 1156 486
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7936
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5667 6625 442
Size: 416 53 83 399 24 4250 958 1753
Fragments arranged by size:
4250 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7936
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2232 2270 2370 2415 3243 3938 4909 6596
Size: 993 38 100 45 828 695 971 1687
Cuts at: 6596 1239
Size: 2579
Fragments arranged by size:
2579 1687 993 971 828 695 100 45
38
Bpu10I CC'TnA_GC
Cuts at: 2060 2559 2577 2595 2613 2625 2631 2691 2697
Size: 499 18 18 18 12 6 60 6
Cuts at: 2697 2709 2727 3051 3217 2060
Size: 12 18 324 166 6779
Fragments arranged by size:
6779 499 324 166 60 18 18 18
18 12 12 6 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2104 6061 6926 7224 2104
Size: 3957 865 298 2816
Fragments arranged by size:
3957 2816 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2284 5009 6599 882
Size: 617 785 2725 1590 2219
Fragments arranged by size:
2725 2219 1590 785 617
BsaAI yAC'GTr
Cuts at: 493 1204 2468 4869 5727 493
Size: 711 1264 2401 858 2702
Fragments arranged by size:
2702 2401 1264 858 711
BsaBI GATnn'nnATC
Cuts at: 7930 7930
Size: 7936
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2209 5743 6125 6760
Size: 53 83 186 936 676 3534 382 635
Cuts at: 6760 275
Size: 1451
Fragments arranged by size:
3534 1451 936 676 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6369 6767 7041 7188 7599 6369
Size: 398 274 147 411 6706
Fragments arranged by size:
6706 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2487 2517 2956 2986 3010
Size: 30 165 30 1084 30 439 30 24
Cuts at: 3010 3040 3977 4007 1178
Size: 30 937 30 5107
Fragments arranged by size:
5107 1084 937 439 165 30 30 30
30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2142 2172 2235 2298 2319 2322 2420 3425
Size: 956 30 63 63 21 3 98 1005
Cuts at: 3425 3428 3467 3482 4775 4912 5150 1186
Size: 3 39 15 1293 137 238 3972
Fragments arranged by size:
3972 1293 1005 956 238 137 98 63
63 39 30 21 15 3 3
BseYI C'CCAG_C
Cuts at: 2800 3003 4731 7139 7635 2800
Size: 203 1728 2408 496 3101
Fragments arranged by size:
3101 2408 1728 496 203
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7363
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2198 5386 6147 7175 1540
Size: 275 186 197 3188 761 1028 2301
Fragments arranged by size:
3188 2301 1028 761 275 197 186
BsiHKAI G_wGCw'C
Cuts at: 2061 4821 5997 6082 7153 2061
Size: 2760 1176 85 1071 2844
Fragments arranged by size:
2844 2760 1176 1071 85
BsmI GAATG_Cn'
Cuts at: 2364 5447 5540 2364
Size: 3083 93 4760
Fragments arranged by size:
4760 3083 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3398 3825 5215 3398
Size: 427 1390 6119
Fragments arranged by size:
6119 1390 427
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2276 3459 3666 4821 5217 5255
Size: 399 271 215 1183 207 1155 396 38
Cuts at: 5255 5997 6082 7153 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1183 1155 1071 742 399 396 271
215 207 85 38
BspEI T'CCGG_A
Cuts at: 7599 7599
Size: 7936
BspHI T'CATG_A
Cuts at: 5825 7555 5825
Size: 1730 6206
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2815 3079 3217 5291 844
Size: 1971 264 138 2074 3489
Fragments arranged by size:
3489 2074 1971 264 138
BsrBI CCG'CTC
Cuts at: 1394 1823 3568 5823 1394
Size: 429 1745 2255 3507
Fragments arranged by size:
3507 2255 1745 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6431 6605 65
Size: 1183 5183 174 1396
Fragments arranged by size:
5183 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6580 6767 1700
Size: 110 4770 187 2869
Fragments arranged by size:
4770 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7936
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7936
BssSI C'ACGA_G
Cuts at: 5218 5996 7008 5218
Size: 778 1012 6146
Fragments arranged by size:
6146 1012 778
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3071 794
Size: 2277 5659
BstBI TT'CG_AA
Cuts at: 4304 6740 4304
Size: 2436 5500
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7936
BstYI r'GATC_y
Cuts at: 1972 3418 4742 4900 5036 5266 5633 6018 6035
Size: 1446 1324 158 136 230 367 385 17
Cuts at: 6035 7487 7573 7585 7931 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1446 1324 385 367 346 230
158 136 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2192 3539 3870 513
Size: 552 939 188 1347 331 4579
Fragments arranged by size:
4579 1347 939 552 331 188
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7197
Fragments arranged by size:
7197 683 56
BtsI GCAGTG_nn'
Cuts at: 2124 5452 6246 6266 2124
Size: 3328 794 20 3794
Fragments arranged by size:
3794 3328 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7936
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7901
DraI TTT'AAA
Cuts at: 5240 5240
Size: 7936
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3724 6943 809
Size: 507 2408 3219 1802
Fragments arranged by size:
3219 2408 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2195 4272 5383
Size: 54 1425 50 275 18 365 2077 1111
Cuts at: 5383 6272 7901 8
Size: 889 1629 43
Fragments arranged by size:
2077 1629 1425 1111 889 365 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2195 5383 1537
Size: 275 383 3188 4090
Fragments arranged by size:
4090 3188 383 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3345 3381 3423 3459 3821 4662 5866 1353
Size: 1992 36 42 36 362 841 1204 3423
Fragments arranged by size:
3423 1992 1204 841 362 42 36 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 6523 6897 7043 387
Size: 1064 138 435 4499 374 146 1280
Fragments arranged by size:
4499 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2059 2059
Size: 7936
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2232 2270 2370 2415 2859 3243 3405
Size: 393 600 38 100 45 444 384 162
Cuts at: 3405 3498 3938 4665 4909 4981 5999 6596 7383
Size: 93 440 727 244 72 1018 597 787
Cuts at: 7383 7651 1239
Size: 268 1524
Fragments arranged by size:
1524 1018 787 727 600 597 444 440
393 384 268 244 162 100 93 72
45 38
EcoNI CCTnn'n_nnAGG
Cuts at: 2247 2250 2247
Size: 3 7933
EcoO109I rG'GnC_Cy
Cuts at: 2177 2273 4710 2177
Size: 96 2437 5403
Fragments arranged by size:
5403 2437 96
EcoRI G'AATT_C
Cuts at: 5245 5623 5245
Size: 378 7558
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4194 4790 4194
Size: 596 7340
FspI TGC'GCA
Cuts at: 2883 6442 2883
Size: 3559 4377
HaeII r_GCGC'y
Cuts at: 1678 7083 1678
Size: 5405 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2134 2166 3010 3042 3836
Size: 32 135 32 562 32 844 32 794
Cuts at: 3836 3868 4358 4390 4424 4456 6577 6609 6651
Size: 32 490 32 34 32 2121 32 42
Cuts at: 6651 6683 1373
Size: 32 2626
Fragments arranged by size:
2626 2121 844 794 562 490 135 42
34 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3646 5280 5526 669
Size: 988 281 1708 1634 246 3079
Fragments arranged by size:
3079 1708 1634 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4772 748
Size: 4024 3912
HpaI GTT'AAC
Cuts at: 5526 5526
Size: 7936
KpnI G_GTAC'C
Cuts at: 3095 5261 3095
Size: 2166 5770
MfeI C'AATT_G
Cuts at: 4227 5535 4227
Size: 1308 6628
MluI A'CGCG_T
Cuts at: 7812 7812
Size: 7936
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2752 3740 7050 7234 1305
Size: 1447 988 3310 184 2007
Fragments arranged by size:
3310 2007 1447 988 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6114
Fragments arranged by size:
6114 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2987 4867 5894 6253 6412 518
Size: 648 1821 1880 1027 359 159 2042
Fragments arranged by size:
2042 1880 1821 1027 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4277 4731 5352 6029 7177 7422 1606
Size: 2671 454 621 677 1148 245 2120
Fragments arranged by size:
2671 2120 1148 677 621 454 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3539 3870 513
Size: 552 939 1535 331 4579
Fragments arranged by size:
4579 1535 939 552 331
NdeI CA'TA_TG
Cuts at: 387 5161 387
Size: 4774 3162
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7936
NotI GC'GGCC_GC
Cuts at: 5383 5383
Size: 7936
NspI r_CATG'y
Cuts at: 1132 1229 3124 3949 4205 5294 6839 1132
Size: 97 1895 825 256 1089 1545 2229
Fragments arranged by size:
2229 1895 1545 1089 825 256 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7936
PciI A'CATG_T
Cuts at: 6835 6835
Size: 7936
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2162 1108
Size: 1054 6882
PfoI T'CCnGG_A
Cuts at: 2248 2248
Size: 7936
PmeI GTTT'AAAC
Cuts at: 5240 5240
Size: 7936
PmlI CAC'GTG
Cuts at: 2468 4869 2468
Size: 2401 5535
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6005 6037 7518 7550 6005
Size: 32 1481 32 6391
Fragments arranged by size:
6391 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4710 4710
Size: 7936
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7936
PsiI TTA'TAA
Cuts at: 2454 3285 3582 5027 5506 2454
Size: 831 297 1445 479 4884
Fragments arranged by size:
4884 1445 831 479 297
PspOMI G'GGCC_C
Cuts at: 2272 2272
Size: 7936
PstI C_TGCA'G
Cuts at: 830 2812 5288 830
Size: 1982 2476 3478
Fragments arranged by size:
3478 2476 1982
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7936
SacI G_AGCT'C
Cuts at: 2061 2061
Size: 7936
SalI G'TCGA_C
Cuts at: 1936 5278 1936
Size: 3342 4594
SapI GCTCTTCn'nnn_
Cuts at: 3381 3423 4662 3381
Size: 42 1239 6655
Fragments arranged by size:
6655 1239 42
SbfI CC_TGCA'GG
Cuts at: 5288 5288
Size: 7936
ScaI AGT'ACT
Cuts at: 5118 6184 7896 5118
Size: 1066 1712 5158
Fragments arranged by size:
5158 1712 1066
SfcI C'TryA_G
Cuts at: 826 1045 2808 2949 2976 5284 6419 7100 7291
Size: 219 1763 141 27 2308 1135 681 191
Cuts at: 7291 826
Size: 1471
Fragments arranged by size:
2308 1763 1471 1135 681 219 191 141
27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7936
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7936
SmaI CCC'GGG
Cuts at: 1872 2052 5263 1872
Size: 180 3211 4545
Fragments arranged by size:
4545 3211 180
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2083 6040 6941 7203 820
Size: 197 926 140 3957 901 262 1553
Fragments arranged by size:
3957 1553 926 901 262 197 140
SnaBI TAC'GTA
Cuts at: 493 5727 493
Size: 5234 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7936
SphI G_CATG'C
Cuts at: 1132 3124 4205 5294 1132
Size: 1992 1081 1089 3774
Fragments arranged by size:
3774 1992 1089 1081
SspI AAT'ATT
Cuts at: 5 52 5860 5
Size: 47 5808 2081
Fragments arranged by size:
5808 2081 47
StuI AGG'CCT
Cuts at: 1517 2081 1517
Size: 564 7372
StyI C'CwwG_G
Cuts at: 513 1065 2004 3539 3870 4125 5375 513
Size: 552 939 1535 331 255 1250 3074
Fragments arranged by size:
3074 1535 1250 939 552 331 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2914 3627 3723 4260 5963 5980 6133
Size: 1255 1334 713 96 537 1703 17 153
Cuts at: 6133 6318 6763 325
Size: 185 445 1498
Fragments arranged by size:
1703 1498 1334 1255 713 537 445 185
153 96 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4467 5116 6182
Size: 274 80 33 51 1443 2490 649 1066
Cuts at: 6182 7894 96
Size: 1712 138
Fragments arranged by size:
2490 1712 1443 1066 649 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7896 7928 7896
Size: 32 7904
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7936
XbaI T'CTAG_A
Cuts at: 5272 5272
Size: 7936
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3913 3913
Size: 7936
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7936
XmaI C'CCGG_G
Cuts at: 1870 2050 5261 1870
Size: 180 3211 4545
Fragments arranged by size:
4545 3211 180
XmnI GAAnn'nnTTC
Cuts at: 3935 6065 3935
Size: 2130 5806
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2210 5744 276
Size: 53 83 186 936 676 3534 2468
Fragments arranged by size:
3534 2468 936 676 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI
PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI