Restriction map for
pFN21AB5636
(Circular) (Six-base) MAPSORT of: pFN21AB5636.seq Check: 9829 from: 1 to: 5698
pFN21AB5636 5698 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3508 278
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3041 1937
Size: 1104 4594
Acc65I G'GTAC_C
Cuts at: 3019 3019
Size: 5698
AclI AA'CG_TT
Cuts at: 1563 3825 4198 1563
Size: 2262 373 3063
Fragments arranged by size:
3063 2262 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3761 5145 5413 1632
Size: 2129 1384 268 1917
Fragments arranged by size:
2129 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5501
AflIII A'CryG_T
Cuts at: 1203 4597 5574 1203
Size: 3394 977 1327
Fragments arranged by size:
3394 1327 977
AgeI A'CCGG_T
Cuts at: 4529 4529
Size: 5698
AhdI GACnn_n'nnGTC
Cuts at: 4427 4427
Size: 5698
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5698
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5698
AlwNI CAG_nnn'CTG
Cuts at: 2434 3441 5013 2434
Size: 1007 1572 3119
Fragments arranged by size:
3119 1572 1007
ApaI G_GGCC'C
Cuts at: 2301 2301
Size: 5698
ApaLI G'TGCA_C
Cuts at: 3755 4911 3755
Size: 1156 4542
ApoI r'AATT_y
Cuts at: 1493 3007 3233 3385 5494 1493
Size: 1514 226 152 2109 1697
Fragments arranged by size:
2109 1697 1514 226 152
AscI GG'CGCG_CC
Cuts at: 2826 2826
Size: 5698
AseI AT'TA_AT
Cuts at: 160 1032 4252 160
Size: 872 3220 1606
Fragments arranged by size:
3220 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
AvaI C'yCGr_G
Cuts at: 1870 1943 2182 2294 2994 3023 1870
Size: 73 239 112 700 29 4545
Fragments arranged by size:
4545 700 239 112 73 29
BamHI G'GATC_C
Cuts at: 3028 3395 3028
Size: 367 5331
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2210 2266 2753 3019 4474
Size: 325 217 627 423 56 487 266 1455
Cuts at: 4474 618
Size: 1842
Fragments arranged by size:
1842 1455 627 487 423 325 266 217
56
BanII G_rGCy'C
Cuts at: 1391 2151 2301 2634 2928 3017 1391
Size: 760 150 333 294 89 4072
Fragments arranged by size:
4072 760 333 294 150 89
BbeI G_GCGC'C
Cuts at: 2214 2214
Size: 5698
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4727
BbvCI CC'TCA_GC
Cuts at: 2423 2423
Size: 5698
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3889 3923 1903
Size: 34 1952 34 3678
Fragments arranged by size:
3678 1952 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3590 4800 3590
Size: 1210 4488
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5698
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2081 2187 4309
Size: 107 122 71 631 605 409 106 2122
Cuts at: 4309 136
Size: 1525
Fragments arranged by size:
2122 1525 631 605 409 122 107 106
71
BglII A'GATC_T
Cuts at: 5693 5693
Size: 5698
BlpI GC'TnA_GC
Cuts at: 2672 3115 2672
Size: 443 5255
Bme1580I G_kGCm'C
Cuts at: 1790 2271 2301 2756 3759 4915 1790
Size: 481 30 455 1003 1156 2573
Fragments arranged by size:
2573 1156 1003 481 455 30
BmgBI CAC'GTC
Cuts at: 1318 2854 1318
Size: 1536 4162
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2147 2174 3429
Size: 416 53 83 399 24 730 27 1255
Cuts at: 3429 4387 442
Size: 958 1753
Fragments arranged by size:
1753 1255 958 730 416 399 83 53
27 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2854 4358 1239
Size: 1615 1504 2579
Fragments arranged by size:
2579 1615 1504
Bpu10I CC'TnA_GC
Cuts at: 2423 2423
Size: 5698
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3823 4688 4986 3823
Size: 865 298 4535
Fragments arranged by size:
4535 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2506 4361 882
Size: 617 1007 1855 2219
Fragments arranged by size:
2219 1855 1007 617
BsaAI yAC'GTr
Cuts at: 493 1204 3489 493
Size: 711 2285 2702
Fragments arranged by size:
2702 2285 711
BsaBI GATnn'nnATC
Cuts at: 5692 5692
Size: 5698
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2112 2211 3505 3887
Size: 53 83 186 936 579 99 1294 382
Cuts at: 3887 4522 275
Size: 635 1451
Fragments arranged by size:
1451 1294 936 635 579 382 186 99
83 53
BsaWI w'CCGG_w
Cuts at: 4131 4529 4803 4950 5361 4131
Size: 398 274 147 411 4468
Fragments arranged by size:
4468 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5473
Fragments arranged by size:
5473 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2524 2759 2762 2936 1186
Size: 1338 235 3 174 3948
Fragments arranged by size:
3948 1338 235 174 3
BseYI C'CCAG_C
Cuts at: 2046 2757 4901 5397 2046
Size: 711 2144 496 2347
Fragments arranged by size:
2347 2144 711 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2305 2381 1163
Size: 573 569 76 4480
Fragments arranged by size:
4480 573 569 76
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2018 2070 2139 2250 2353 2404
Size: 275 186 17 52 69 111 103 51
Cuts at: 2404 3148 3909 4937 1540
Size: 744 761 1028 2301
Fragments arranged by size:
2301 1028 761 744 275 186 111 103
69 52 51 17
BsiHKAI G_wGCw'C
Cuts at: 2634 3759 3844 4915 2634
Size: 1125 85 1071 3417
Fragments arranged by size:
3417 1125 1071 85
BsmI GAATG_Cn'
Cuts at: 3209 3302 3209
Size: 93 5605
BsmBI CGTCTCn'nnnn_
Cuts at: 2117 2117
Size: 5698
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2151 2271 2301 2634 2756 2928 3017
Size: 399 361 120 30 333 122 172 89
Cuts at: 3017 3759 3844 4915 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 361 333 172 122
120 89 85 30
BspEI T'CCGG_A
Cuts at: 5361 5361
Size: 5698
BspHI T'CATG_A
Cuts at: 3587 5317 3587
Size: 1730 3968
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3053 844
Size: 2209 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2014 3585 1394
Size: 429 191 1571 3507
Fragments arranged by size:
3507 1571 429 191
BsrDI GCAATG_nn'
Cuts at: 65 1248 4193 4367 65
Size: 1183 2945 174 1396
Fragments arranged by size:
2945 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2198 2987 4342 4529 1700
Size: 110 388 789 1355 187 2869
Fragments arranged by size:
2869 1355 789 388 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5698
BssHII G'CGCG_C
Cuts at: 1925 2169 2171 2255 2406 2826 1925
Size: 244 2 84 151 420 4797
Fragments arranged by size:
4797 420 244 151 84 2
BssSI C'ACGA_G
Cuts at: 3758 4770 3758
Size: 1012 4686
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2854 794
Size: 2060 3638
BstBI TT'CG_AA
Cuts at: 4502 4502
Size: 5698
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5698
BstYI r'GATC_y
Cuts at: 1972 2685 3028 3395 3780 3797 5249 5335 5347
Size: 713 343 367 385 17 1452 86 12
Cuts at: 5347 5693 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 713 385 367 346 343 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2608 487
Size: 683 56 1382 3577
Fragments arranged by size:
3577 1382 683 56
BtsI GCAGTG_nn'
Cuts at: 2044 3214 4008 4028 2044
Size: 1170 794 20 3714
Fragments arranged by size:
3714 1170 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5698
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5663
DraI TTT'AAA
Cuts at: 3002 3002
Size: 5698
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4705 809
Size: 507 3389 1802
Fragments arranged by size:
3389 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2015 2067 2136
Size: 54 1425 50 275 18 185 52 69
Cuts at: 2136 2247 2350 2401 2697 2721 3145 4034 5663
Size: 111 103 51 296 24 424 889 1629
Cuts at: 5663 8
Size: 43
Fragments arranged by size:
1629 1425 889 424 296 275 185 111
103 69 54 52 51 50 43 24
18
EagI C'GGCC_G
Cuts at: 1537 1812 2015 2067 2136 2247 2350 2401 3145
Size: 275 203 52 69 111 103 51 744
Cuts at: 3145 1537
Size: 4090
Fragments arranged by size:
4090 744 275 203 111 103 69 52
51
EarI CTCTTCn'nnn_
Cuts at: 1353 2501 3628 1353
Size: 1148 1127 3423
Fragments arranged by size:
3423 1148 1127
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2681 4285 4659 4805 387
Size: 1064 138 1092 1604 374 146 1280
Fragments arranged by size:
1604 1280 1092 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2632 2632
Size: 5698
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2854 3761 4358 5145 5413 1239
Size: 393 1222 907 597 787 268 1524
Fragments arranged by size:
1524 1222 907 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2619 2779 2619
Size: 160 5538
EcoRI G'AATT_C
Cuts at: 3007 3385 3007
Size: 378 5320
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2873 2873
Size: 5698
FspI TGC'GCA
Cuts at: 4204 4204
Size: 5698
HaeII r_GCGC'y
Cuts at: 1678 2214 4845 1678
Size: 536 2631 2531
Fragments arranged by size:
2631 2531 536
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4339 4371 4413 4445 1373
Size: 32 135 32 2767 32 42 32 2626
Fragments arranged by size:
2767 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3042 3288 669
Size: 988 281 1104 246 3079
Fragments arranged by size:
3079 1104 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5698
HpaI GTT'AAC
Cuts at: 3288 3288
Size: 5698
KasI G'GCGC_C
Cuts at: 2210 2210
Size: 5698
KpnI G_GTAC'C
Cuts at: 3023 3023
Size: 5698
MfeI C'AATT_G
Cuts at: 3297 3297
Size: 5698
MluI A'CGCG_T
Cuts at: 5574 5574
Size: 5698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4812 4996 1305
Size: 3507 184 2007
Fragments arranged by size:
3507 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2699 2723 10
Size: 54 1425 343 867 24 2985
Fragments arranged by size:
2985 1425 867 343 54 24
MslI CAynn'nnrTG
Cuts at: 518 1166 3656 4015 4174 518
Size: 648 2490 359 159 2042
Fragments arranged by size:
2490 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2144 2333 2365 2434 2451 2761 2874 3114
Size: 538 189 32 69 17 310 113 240
Cuts at: 3114 3791 4939 5184 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 538 310 245 240 189
113 69 32 17
NaeI GCC'GGC
Cuts at: 2989 2989
Size: 5698
NarI GG'CG_CC
Cuts at: 2211 2211
Size: 5698
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5146
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5698
NgoMIV G'CCGG_C
Cuts at: 2987 2987
Size: 5698
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5698
NotI GC'GGCC_GC
Cuts at: 2015 2136 3145 2015
Size: 121 1009 4568
Fragments arranged by size:
4568 1009 121
NspI r_CATG'y
Cuts at: 1132 1229 3056 4601 1132
Size: 97 1827 1545 2229
Fragments arranged by size:
2229 1827 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5698
PciI A'CATG_T
Cuts at: 4597 4597
Size: 5698
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2434 1108
Size: 1326 4372
PmeI GTTT'AAAC
Cuts at: 3002 3002
Size: 5698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3767 3799 5280 5312 3767
Size: 32 1481 32 4153
Fragments arranged by size:
4153 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2779 2779
Size: 5698
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5698
PsiI TTA'TAA
Cuts at: 3268 3268
Size: 5698
PspOMI G'GGCC_C
Cuts at: 2297 2297
Size: 5698
PstI C_TGCA'G
Cuts at: 830 3050 830
Size: 2220 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5698
PvuII CAG'CTG
Cuts at: 2365 2874 2365
Size: 509 5189
SacI G_AGCT'C
Cuts at: 2634 2634
Size: 5698
SalI G'TCGA_C
Cuts at: 1936 3040 1936
Size: 1104 4594
SbfI CC_TGCA'GG
Cuts at: 3050 3050
Size: 5698
ScaI AGT'ACT
Cuts at: 3946 5658 3946
Size: 1712 3986
SexAI A'CCwGG_T
Cuts at: 2094 2094
Size: 5698
SfcI C'TryA_G
Cuts at: 826 1045 2416 3046 4181 4862 5053 826
Size: 219 1371 630 1135 681 191 1471
Fragments arranged by size:
1471 1371 1135 681 630 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2081 2081
Size: 5698
SfoI GGC'GCC
Cuts at: 2212 2212
Size: 5698
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5698
SmaI CCC'GGG
Cuts at: 1872 2184 2296 3025 1872
Size: 312 112 729 4545
Fragments arranged by size:
4545 729 312 112
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3802 4703 4965 820
Size: 197 926 1859 901 262 1553
Fragments arranged by size:
1859 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3489 493
Size: 2996 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5698
SphI G_CATG'C
Cuts at: 1132 3056 1132
Size: 1924 3774
SspI AAT'ATT
Cuts at: 5 52 3622 5
Size: 47 3570 2081
Fragments arranged by size:
3570 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5698
StyI C'CwwG_G
Cuts at: 513 1065 2118 3137 513
Size: 552 1053 1019 3074
Fragments arranged by size:
3074 1053 1019 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2024 2771 3725 3742 3895 4080 4525
Size: 1255 444 747 954 17 153 185 445
Cuts at: 4525 325
Size: 1498
Fragments arranged by size:
1498 1255 954 747 445 444 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3944 5656 96
Size: 274 80 33 51 1443 1967 1712 138
Fragments arranged by size:
1967 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5658 5690 5658
Size: 32 5666
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5698
XbaI T'CTAG_A
Cuts at: 3034 3034
Size: 5698
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5698
XmaI C'CCGG_G
Cuts at: 1870 2182 2294 3023 1870
Size: 312 112 729 4545
Fragments arranged by size:
4545 729 312 112
XmnI GAAnn'nnTTC
Cuts at: 3827 3827
Size: 5698
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3506 276
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BfrBI BplI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacII SanDI SapI SrfI SwaI XcmI