Restriction map for
pFN21AB5633
(Circular) (Six-base) MAPSORT of: pFN21AB5633.seq Check: 9771 from: 1 to: 6271
pFN21AB5633 6271 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4081 278
Size: 53 83 186 936 2545 2468
Fragments arranged by size:
2545 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3614 1937
Size: 1677 4594
Acc65I G'GTAC_C
Cuts at: 3592 3592
Size: 6271
AclI AA'CG_TT
Cuts at: 1563 4398 4771 1563
Size: 2835 373 3063
Fragments arranged by size:
3063 2835 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2561 3173 3339 4334 5718 5986 1632
Size: 929 612 166 995 1384 268 1917
Fragments arranged by size:
1917 1384 995 929 612 268 166
AfeI AGC'GCT
Cuts at: 2089 3209 2089
Size: 1120 5151
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6074
AflIII A'CryG_T
Cuts at: 1203 5170 6147 1203
Size: 3967 977 1327
Fragments arranged by size:
3967 1327 977
AgeI A'CCGG_T
Cuts at: 5102 5102
Size: 6271
AhdI GACnn_n'nnGTC
Cuts at: 5000 5000
Size: 6271
AleI CACnn'nnGTG
Cuts at: 1166 3046 1166
Size: 1880 4391
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3064 3197 1184
Size: 1880 133 4258
Fragments arranged by size:
4258 1880 133
AlwNI CAG_nnn'CTG
Cuts at: 2082 4014 5586 2082
Size: 1932 1572 2767
Fragments arranged by size:
2767 1932 1572
ApaLI G'TGCA_C
Cuts at: 4328 5484 4328
Size: 1156 5115
ApoI r'AATT_y
Cuts at: 1493 2516 2884 3057 3580 3806 3958 6067 1493
Size: 1023 368 173 523 226 152 2109 1697
Fragments arranged by size:
2109 1697 1023 523 368 226 173 152
AseI AT'TA_AT
Cuts at: 160 1032 2526 4825 160
Size: 872 1494 2299 1606
Fragments arranged by size:
2299 1606 1494 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6271
AvaI C'yCGr_G
Cuts at: 1870 1943 3158 3596 1870
Size: 73 1215 438 4545
Fragments arranged by size:
4545 1215 438 73
BamHI G'GATC_C
Cuts at: 2036 3601 3968 2036
Size: 1565 367 4339
Fragments arranged by size:
4339 1565 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3592 5047 618
Size: 325 217 627 1805 1455 1842
Fragments arranged by size:
1842 1805 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2994 3590 1391
Size: 1603 596 4072
Fragments arranged by size:
4072 1603 596
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5300
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4462 4496 1903
Size: 34 2525 34 3678
Fragments arranged by size:
3678 2525 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4163 5373 4163
Size: 1210 5061
BclI T'GATC_A
Cuts at: 1548 3310 1548
Size: 1762 4509
BfrBI ATG'CAT
Cuts at: 3091 3436 3091
Size: 345 5926
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4882 136
Size: 107 122 71 631 605 3210 1525
Fragments arranged by size:
3210 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6266 6266
Size: 6271
BlpI GC'TnA_GC
Cuts at: 3204 3688 3204
Size: 484 5787
Bme1580I G_kGCm'C
Cuts at: 1790 4332 5488 1790
Size: 2542 1156 2573
Fragments arranged by size:
2573 2542 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6271
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2797 4002 4960
Size: 416 53 83 399 24 1380 1205 958
Cuts at: 4960 442
Size: 1753
Fragments arranged by size:
1753 1380 1205 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6271
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2125 4931 1239
Size: 886 2806 2579
Fragments arranged by size:
2806 2579 886
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2468 3035 4396 5261 5559 2468
Size: 567 1361 865 298 3180
Fragments arranged by size:
3180 1361 865 567 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3505 4934 882
Size: 617 2006 1429 2219
Fragments arranged by size:
2219 2006 1429 617
BsaAI yAC'GTr
Cuts at: 493 1204 4062 493
Size: 711 2858 2702
Fragments arranged by size:
2858 2702 711
BsaBI GATnn'nnATC
Cuts at: 6265 6265
Size: 6271
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4078 4460 5095 275
Size: 53 83 186 936 2545 382 635 1451
Fragments arranged by size:
2545 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2880 4704 5102 5376 5523 5934 2880
Size: 1824 398 274 147 411 3217
Fragments arranged by size:
3217 1824 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3523 3553 1178
Size: 30 165 30 2120 30 3896
Fragments arranged by size:
3896 2120 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2171 3125 1186
Size: 985 954 4332
Fragments arranged by size:
4332 985 954
BseYI C'CCAG_C
Cuts at: 5474 5970 5474
Size: 496 5775
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5698
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3721 4482 5510 1540
Size: 275 186 1720 761 1028 2301
Fragments arranged by size:
2301 1720 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2994 4332 4417 5488 2994
Size: 1338 85 1071 3777
Fragments arranged by size:
3777 1338 1071 85
BsmI GAATG_Cn'
Cuts at: 2216 3093 3782 3875 2216
Size: 877 689 93 4612
Fragments arranged by size:
4612 877 689 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2994 3590 4332 4417 5488 1391
Size: 399 1204 596 742 85 1071 2174
Fragments arranged by size:
2174 1204 1071 742 596 399 85
BspEI T'CCGG_A
Cuts at: 2880 5934 2880
Size: 3054 3217
BspHI T'CATG_A
Cuts at: 4160 5890 4160
Size: 1730 4541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2297 3626 844
Size: 1453 1329 3489
Fragments arranged by size:
3489 1453 1329
BsrBI CCG'CTC
Cuts at: 1394 1823 4158 1394
Size: 429 2335 3507
Fragments arranged by size:
3507 2335 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3058 4766 4940 65
Size: 1183 1810 1708 174 1396
Fragments arranged by size:
1810 1708 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4915 5102 1700
Size: 110 3105 187 2869
Fragments arranged by size:
3105 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6271
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6271
BssSI C'ACGA_G
Cuts at: 4331 5343 4331
Size: 1012 5259
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2280 794
Size: 1486 4785
BstBI TT'CG_AA
Cuts at: 3007 5075 3007
Size: 2068 4203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6271
BstYI r'GATC_y
Cuts at: 1972 2036 2160 2610 3601 3968 4353 4370 5822
Size: 64 124 450 991 367 385 17 1452
Cuts at: 5822 5908 5920 6266 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 991 450 385 367 346 124
86 64 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2070 3398 513
Size: 552 1005 1328 3386
Fragments arranged by size:
3386 1328 1005 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5532
Fragments arranged by size:
5532 683 56
BtsI GCAGTG_nn'
Cuts at: 2250 3053 3787 4581 4601 2250
Size: 803 734 794 20 3920
Fragments arranged by size:
3920 803 794 734 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6271
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3254 3289 3493 3528 529
Size: 35 2690 35 204 35 3272
Fragments arranged by size:
3272 2690 204 35 35 35
DraI TTT'AAA
Cuts at: 3575 3575
Size: 6271
DraIII CAC_nnn'GTG
Cuts at: 2468 2468
Size: 6271
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5278 809
Size: 507 3962 1802
Fragments arranged by size:
3962 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2067 2073 2260
Size: 54 1425 50 275 18 237 6 187
Cuts at: 2260 3718 4607 6236 8
Size: 1458 889 1629 43
Fragments arranged by size:
1629 1458 1425 889 275 237 187 54
50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 3718 1537
Size: 275 1906 4090
Fragments arranged by size:
4090 1906 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3102 3339 4201 1353
Size: 1749 237 862 3423
Fragments arranged by size:
3423 1749 862 237
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4858 5232 5378 387
Size: 1064 138 3269 374 146 1280
Fragments arranged by size:
3269 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2992 2992
Size: 6271
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2125 2561 3173 3339 4334 4931 5718
Size: 393 493 436 612 166 995 597 787
Cuts at: 5718 5986 1239
Size: 268 1524
Fragments arranged by size:
1524 995 787 612 597 493 436 393
268 166
EcoRI G'AATT_C
Cuts at: 3580 3958 3580
Size: 378 5893
FspI TGC'GCA
Cuts at: 4777 4777
Size: 6271
HaeII r_GCGC'y
Cuts at: 1678 2091 3211 5418 1678
Size: 413 1120 2207 2531
Fragments arranged by size:
2531 2207 1120 413
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2374 2406 2674 2706 3378
Size: 32 135 32 802 32 268 32 672
Cuts at: 3378 3410 4912 4944 4986 5018 1373
Size: 32 1502 32 42 32 2626
Fragments arranged by size:
2626 1502 802 672 268 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2326 3615 3861 669
Size: 988 281 388 1289 246 3079
Fragments arranged by size:
3079 1289 988 388 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6271
HpaI GTT'AAC
Cuts at: 3861 3861
Size: 6271
KpnI G_GTAC'C
Cuts at: 3596 3596
Size: 6271
MfeI C'AATT_G
Cuts at: 3870 3870
Size: 6271
MluI A'CGCG_T
Cuts at: 6147 6147
Size: 6271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5385 5569 1305
Size: 4080 184 2007
Fragments arranged by size:
4080 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2069 2075 10
Size: 54 1425 343 237 6 4206
Fragments arranged by size:
4206 1425 343 237 54 6
MslI CAynn'nnrTG
Cuts at: 518 1166 2816 3046 4229 4588 4747 518
Size: 648 1650 230 1183 359 159 2042
Fragments arranged by size:
2042 1650 1183 648 359 230 159
MspA1I CmG'CkG
Cuts at: 1606 2031 3687 4364 5512 5757 1606
Size: 425 1656 677 1148 245 2120
Fragments arranged by size:
2120 1656 1148 677 425 245
NcoI C'CATG_G
Cuts at: 513 1065 2070 513
Size: 552 1005 4714
Fragments arranged by size:
4714 1005 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6271
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6271
NotI GC'GGCC_GC
Cuts at: 3718 3718
Size: 6271
NsiI A_TGCA'T
Cuts at: 3093 3438 3093
Size: 345 5926
NspI r_CATG'y
Cuts at: 1132 1229 3178 3629 5174 1132
Size: 97 1949 451 1545 2229
Fragments arranged by size:
2229 1949 1545 451 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6271
PciI A'CATG_T
Cuts at: 5170 5170
Size: 6271
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6271
PfoI T'CCnGG_A
Cuts at: 2889 2943 2889
Size: 54 6217
PmeI GTTT'AAAC
Cuts at: 3575 3575
Size: 6271
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4340 4372 5853 5885 4340
Size: 32 1481 32 4726
Fragments arranged by size:
4726 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6271
PsiI TTA'TAA
Cuts at: 3841 3841
Size: 6271
PstI C_TGCA'G
Cuts at: 830 3623 830
Size: 2793 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6271
PvuII CAG'CTG
Cuts at: 2031 2031
Size: 6271
SacI G_AGCT'C
Cuts at: 2994 2994
Size: 6271
SalI G'TCGA_C
Cuts at: 1936 3613 1936
Size: 1677 4594
SbfI CC_TGCA'GG
Cuts at: 3623 3623
Size: 6271
ScaI AGT'ACT
Cuts at: 2740 3556 4519 6231 2740
Size: 816 963 1712 2780
Fragments arranged by size:
2780 1712 963 816
SfcI C'TryA_G
Cuts at: 826 1045 2083 2712 3619 4754 5435 5626 826
Size: 219 1038 629 907 1135 681 191 1471
Fragments arranged by size:
1471 1135 1038 907 681 629 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6271
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6271
SmaI CCC'GGG
Cuts at: 1872 3160 3598 1872
Size: 1288 438 4545
Fragments arranged by size:
4545 1288 438
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2447 3014 4375 5276 5538 820
Size: 197 926 504 567 1361 901 262 1553
Fragments arranged by size:
1553 1361 926 901 567 504 262 197
SnaBI TAC'GTA
Cuts at: 493 4062 493
Size: 3569 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6271
SphI G_CATG'C
Cuts at: 1132 3178 3629 1132
Size: 2046 451 3774
Fragments arranged by size:
3774 2046 451
SspI AAT'ATT
Cuts at: 5 52 2530 4195 5
Size: 47 2478 1665 2081
Fragments arranged by size:
2478 2081 1665 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6271
StyI C'CwwG_G
Cuts at: 513 1065 2070 2552 3272 3710 513
Size: 552 1005 482 720 438 3074
Fragments arranged by size:
3074 1005 720 552 482 438
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2102 2816 4298 4315 4468 4653 5098
Size: 1255 522 714 1482 17 153 185 445
Cuts at: 5098 325
Size: 1498
Fragments arranged by size:
1498 1482 1255 714 522 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2738 3554 4517
Size: 274 80 33 51 1443 761 816 963
Cuts at: 4517 6229 96
Size: 1712 138
Fragments arranged by size:
1712 1443 963 816 761 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6231 6263 6231
Size: 32 6239
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6271
XbaI T'CTAG_A
Cuts at: 2701 3607 2701
Size: 906 5365
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2149 2224 2149
Size: 75 6196
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6271
XmaI C'CCGG_G
Cuts at: 1870 3158 3596 1870
Size: 1288 438 4545
Fragments arranged by size:
4545 1288 438
XmnI GAAnn'nnTTC
Cuts at: 2898 4400 2898
Size: 1502 4769
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4079 276
Size: 53 83 186 936 2545 2468
Fragments arranged by size:
2545 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI