Restriction map for
pFN21AB5631
(Circular) (Six-base) MAPSORT of: pFN21AB5631.seq Check: 3952 from: 1 to: 7813
pFN21AB5631 7813 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3553 3723 3553
Size: 170 7643
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5623 278
Size: 53 83 186 936 4087 2468
Fragments arranged by size:
4087 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2124 2838 3325 5156 1937
Size: 187 714 487 1831 4594
Fragments arranged by size:
4594 1831 714 487 187
Acc65I G'GTAC_C
Cuts at: 2293 5134 2293
Size: 2841 4972
AclI AA'CG_TT
Cuts at: 1563 5940 6313 1563
Size: 4377 373 3063
Fragments arranged by size:
4377 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3501 4689 5021 5070 5876 7260 7528 1632
Size: 1869 1188 332 49 806 1384 268 1917
Fragments arranged by size:
1917 1869 1384 1188 806 332 268 49
AfeI AGC'GCT
Cuts at: 3989 3989
Size: 7813
AflII C'TTAA_G
Cuts at: 820 1017 3571 820
Size: 197 2554 5062
Fragments arranged by size:
5062 2554 197
AflIII A'CryG_T
Cuts at: 1203 2133 2442 6712 7689 1203
Size: 930 309 4270 977 1327
Fragments arranged by size:
4270 1327 977 930 309
AgeI A'CCGG_T
Cuts at: 6644 6644
Size: 7813
AhdI GACnn_n'nnGTC
Cuts at: 6542 6542
Size: 7813
AleI CACnn'nnGTG
Cuts at: 1166 3491 1166
Size: 2325 5488
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2070 3948 1184
Size: 886 1878 5049
Fragments arranged by size:
5049 1878 886
AlwNI CAG_nnn'CTG
Cuts at: 3819 4697 5556 7128 3819
Size: 878 859 1572 4504
Fragments arranged by size:
4504 1572 878 859
ApaLI G'TGCA_C
Cuts at: 5870 7026 5870
Size: 1156 6657
ApoI r'AATT_y
Cuts at: 1493 2336 2360 2378 2846 2978 3680 4453 4832
Size: 843 24 18 468 132 702 773 379
Cuts at: 4832 5122 5348 5500 7609 1493
Size: 290 226 152 2109 1697
Fragments arranged by size:
2109 1697 843 773 702 468 379 290
226 152 132 24 18
AseI AT'TA_AT
Cuts at: 160 1032 2304 6367 160
Size: 872 1272 4063 1606
Fragments arranged by size:
4063 1606 1272 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7813
AvaI C'yCGr_G
Cuts at: 1870 1943 2475 4746 5138 1870
Size: 73 532 2271 392 4545
Fragments arranged by size:
4545 2271 532 392 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2210 2243 2277 2310 2210
Size: 33 34 33 7713
Fragments arranged by size:
7713 34 33 33
BamHI G'GATC_C
Cuts at: 4302 4816 5143 5510 4302
Size: 514 327 367 6605
Fragments arranged by size:
6605 514 367 327
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2293 2813 2996 3708 4899
Size: 325 217 627 506 520 183 712 1191
Cuts at: 4899 5134 6589 618
Size: 235 1455 1842
Fragments arranged by size:
1842 1455 1191 712 627 520 506 325
235 217 183
BanII G_rGCy'C
Cuts at: 1391 3391 4542 5132 1391
Size: 2000 1151 590 4072
Fragments arranged by size:
4072 2000 1151 590
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2443 3841 928
Size: 971 544 1398 4900
Fragments arranged by size:
4900 1398 971 544
BbvCI CC'TCA_GC
Cuts at: 4989 4989
Size: 7813
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4274 4308 6004 6038 1903
Size: 34 2337 34 1696 34 3678
Fragments arranged by size:
3678 2337 1696 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5705 6915 5705
Size: 1210 6603
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7813
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6424 136
Size: 107 122 71 631 605 4752 1525
Fragments arranged by size:
4752 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2690 7808 2690
Size: 5118 2695
BlpI GC'TnA_GC
Cuts at: 5230 5230
Size: 7813
Bme1580I G_kGCm'C
Cuts at: 1790 5097 5874 7030 1790
Size: 3307 777 1156 2573
Fragments arranged by size:
3307 2573 1156 777
BmgBI CAC'GTC
Cuts at: 1318 2134 3493 3974 1318
Size: 816 1359 481 5157
Fragments arranged by size:
5157 1359 816 481
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2481 3173 3868
Size: 416 53 83 399 24 1064 692 695
Cuts at: 3868 5544 6502 442
Size: 1676 958 1753
Fragments arranged by size:
1753 1676 1064 958 695 692 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7813
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2411 2411
Size: 7813
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2715 6473 1239
Size: 1476 3758 2579
Fragments arranged by size:
3758 2579 1476
Bpu10I CC'TnA_GC
Cuts at: 4968 4989 4968
Size: 21 7792
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2181 2381 5938 6803 7101 2181
Size: 200 3557 865 298 2893
Fragments arranged by size:
3557 2893 865 298 200
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6476 882
Size: 617 4977 2219
Fragments arranged by size:
4977 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5604 493
Size: 711 4400 2702
Fragments arranged by size:
4400 2702 711
BsaBI GATnn'nnATC
Cuts at: 3134 7807 3134
Size: 4673 3140
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5620 6002 6637 275
Size: 53 83 186 936 4087 382 635 1451
Fragments arranged by size:
4087 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4896 6246 6644 6918 7065 7476 4896
Size: 1350 398 274 147 411 5233
Fragments arranged by size:
5233 1350 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2106 2136 3737 3767 3887
Size: 30 165 30 703 30 1601 30 120
Cuts at: 3887 3917 4107 4137 1178
Size: 30 190 30 4854
Fragments arranged by size:
4854 1601 703 190 165 120 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2132 2483 4461 5006 1186
Size: 946 351 1978 545 3993
Fragments arranged by size:
3993 1978 946 545 351
BseYI C'CCAG_C
Cuts at: 2141 4648 7016 7512 2141
Size: 2507 2368 496 2442
Fragments arranged by size:
2507 2442 2368 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2025 3701 4462 1163
Size: 573 289 1676 761 4514
Fragments arranged by size:
4514 1676 761 573 289
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5263 6024 7052 1540
Size: 275 186 3262 761 1028 2301
Fragments arranged by size:
3262 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3480 4542 5874 5959 7030 3480
Size: 1062 1332 85 1071 4263
Fragments arranged by size:
4263 1332 1071 1062 85
BsmI GAATG_Cn'
Cuts at: 3055 3213 5324 5417 3055
Size: 158 2111 93 5451
Fragments arranged by size:
5451 2111 158 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3531 3531
Size: 7813
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3391 3480 4542 5097 5132 5874 5959
Size: 399 1601 89 1062 555 35 742 85
Cuts at: 5959 7030 1391
Size: 1071 2174
Fragments arranged by size:
2174 1601 1071 1062 742 555 399 89
85 35
BspEI T'CCGG_A
Cuts at: 7476 7476
Size: 7813
BspHI T'CATG_A
Cuts at: 5702 7432 5702
Size: 1730 6083
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3553 3723 5168 844
Size: 2709 170 1445 3489
Fragments arranged by size:
3489 2709 1445 170
BsrBI CCG'CTC
Cuts at: 1394 1823 4128 5700 1394
Size: 429 2305 1572 3507
Fragments arranged by size:
3507 2305 1572 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2817 3114 3558 3930 4290 6308 6482
Size: 1183 1569 297 444 372 360 2018 174
Cuts at: 6482 65
Size: 1396
Fragments arranged by size:
2018 1569 1396 1183 444 372 360 297
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4388 4737 6457 6644 1700
Size: 110 2578 349 1720 187 2869
Fragments arranged by size:
2869 2578 1720 349 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7813
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7813
BssSI C'ACGA_G
Cuts at: 3960 5873 6885 3960
Size: 1913 1012 4888
Fragments arranged by size:
4888 1913 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3715 794
Size: 2921 4892
BstBI TT'CG_AA
Cuts at: 4371 6617 4371
Size: 2246 5567
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7813
BstXI CCAn_nnnn'nTGG
Cuts at: 2993 2993
Size: 7813
BstYI r'GATC_y
Cuts at: 1972 2690 3246 4302 4816 4975 5007 5143 5510
Size: 718 556 1056 514 159 32 136 367
Cuts at: 5510 5895 5912 7364 7450 7462 7808 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1056 718 556 514 385 367
346 159 136 86 32 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2024 3946 5011 5097 513
Size: 552 959 1922 1065 86 3229
Fragments arranged by size:
3229 1922 1065 959 552 86
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4299 487
Size: 683 56 3073 4001
Fragments arranged by size:
4001 3073 683 56
BtsI GCAGTG_nn'
Cuts at: 2042 3505 5329 6123 6143 2042
Size: 1463 1824 794 20 3712
Fragments arranged by size:
3712 1824 1463 794 20
ClaI AT'CG_AT
Cuts at: 1554 3019 1554
Size: 1465 6348
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7778
DraI TTT'AAA
Cuts at: 2341 2928 5117 2341
Size: 587 2189 5037
Fragments arranged by size:
5037 2189 587
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6820 809
Size: 507 5504 1802
Fragments arranged by size:
5504 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4735 5260 6149
Size: 54 1425 50 275 18 2905 525 889
Cuts at: 6149 7778 8
Size: 1629 43
Fragments arranged by size:
2905 1629 1425 889 525 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5260 1537
Size: 275 3448 4090
Fragments arranged by size:
4090 3448 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2731 3108 3120 3614 4161 5743 1353
Size: 1378 377 12 494 547 1582 3423
Fragments arranged by size:
3423 1582 1378 547 494 377 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4407 6400 6774 6920 387
Size: 1064 138 2818 1993 374 146 1280
Fragments arranged by size:
2818 1993 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4540 4540
Size: 7813
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2715 3501 4689 5021 5070 5876 6473
Size: 393 1083 786 1188 332 49 806 597
Cuts at: 6473 7260 7528 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1188 1083 806 787 786 597 393
332 268 49
EcoNI CCTnn'n_nnAGG
Cuts at: 3457 3457
Size: 7813
EcoRI G'AATT_C
Cuts at: 2846 2978 5122 5500 2846
Size: 132 2144 378 5159
Fragments arranged by size:
5159 2144 378 132
EcoRV GAT'ATC
Cuts at: 4085 4085
Size: 7813
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4499 4499
Size: 7813
FspI TGC'GCA
Cuts at: 6319 6319
Size: 7813
HaeII r_GCGC'y
Cuts at: 1678 3991 6960 1678
Size: 2313 2969 2531
Fragments arranged by size:
2969 2531 2313
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3231 3263 3350 3382 3737
Size: 32 135 32 1659 32 87 32 355
Cuts at: 3737 3769 3887 3919 3920 3952 6454 6486 6528
Size: 32 118 32 1 32 2502 32 42
Cuts at: 6528 6560 1373
Size: 32 2626
Fragments arranged by size:
2626 2502 1659 355 135 118 87 42
32 32 32 32 32 32 32 32
32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 5157 5403 669
Size: 988 281 3219 246 3079
Fragments arranged by size:
3219 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3401 748
Size: 2653 5160
HpaI GTT'AAC
Cuts at: 5403 5403
Size: 7813
KpnI G_GTAC'C
Cuts at: 2297 5138 2297
Size: 2841 4972
MfeI C'AATT_G
Cuts at: 5412 5412
Size: 7813
MluI A'CGCG_T
Cuts at: 7689 7689
Size: 7813
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3179 3186 6927 7111 1305
Size: 1874 7 3741 184 2007
Fragments arranged by size:
3741 2007 1874 184 7
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5991
Fragments arranged by size:
5991 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3341 3491 5771 6130 6289 518
Size: 648 2175 150 2280 359 159 2042
Fragments arranged by size:
2280 2175 2042 648 359 159 150
MspA1I CmG'CkG
Cuts at: 1606 2635 2811 4936 5229 5906 7054 7299 1606
Size: 1029 176 2125 293 677 1148 245 2120
Fragments arranged by size:
2125 2120 1148 1029 677 293 245 176
NcoI C'CATG_G
Cuts at: 513 1065 5097 513
Size: 552 4032 3229
Fragments arranged by size:
4032 3229 552
NdeI CA'TA_TG
Cuts at: 387 3031 387
Size: 2644 5169
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7813
NotI GC'GGCC_GC
Cuts at: 5260 5260
Size: 7813
NspI r_CATG'y
Cuts at: 1132 1229 2951 4535 4648 5171 6716 1132
Size: 97 1722 1584 113 523 1545 2229
Fragments arranged by size:
2229 1722 1584 1545 523 113 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7813
PciI A'CATG_T
Cuts at: 6712 6712
Size: 7813
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2653 3424 3819 1108
Size: 1545 771 395 5102
Fragments arranged by size:
5102 1545 771 395
PmeI GTTT'AAAC
Cuts at: 5117 5117
Size: 7813
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5882 5914 7395 7427 5882
Size: 32 1481 32 6268
Fragments arranged by size:
6268 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7813
PsiI TTA'TAA
Cuts at: 2190 4949 5383 2190
Size: 2759 434 4620
Fragments arranged by size:
4620 2759 434
PstI C_TGCA'G
Cuts at: 830 2049 3943 5165 830
Size: 1219 1894 1222 3478
Fragments arranged by size:
3478 1894 1222 1219
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7813
PvuII CAG'CTG
Cuts at: 2635 4936 2635
Size: 2301 5512
SacI G_AGCT'C
Cuts at: 4542 4542
Size: 7813
SalI G'TCGA_C
Cuts at: 1936 5155 1936
Size: 3219 4594
SapI GCTCTTCn'nnn_
Cuts at: 2731 2731
Size: 7813
SbfI CC_TGCA'GG
Cuts at: 5165 5165
Size: 7813
ScaI AGT'ACT
Cuts at: 6061 7773 6061
Size: 1712 6101
SfcI C'TryA_G
Cuts at: 826 1045 2045 2746 2941 2970 3939 4406 4429
Size: 219 1000 701 195 29 969 467 23
Cuts at: 4429 4760 5161 6296 6977 7168 826
Size: 331 401 1135 681 191 1471
Fragments arranged by size:
1471 1135 1000 969 701 681 467 401
331 219 195 191 29 23
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7813
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7813
SmaI CCC'GGG
Cuts at: 1872 5140 1872
Size: 3268 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2196 2396 2475 3571 5917 6818
Size: 197 926 253 200 79 1096 2346 901
Cuts at: 6818 7080 820
Size: 262 1553
Fragments arranged by size:
2346 1553 1096 926 901 262 253 200
197 79
SnaBI TAC'GTA
Cuts at: 493 5604 493
Size: 5111 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7813
SphI G_CATG'C
Cuts at: 1132 4648 5171 1132
Size: 3516 523 3774
Fragments arranged by size:
3774 3516 523
SspI AAT'ATT
Cuts at: 5 52 5737 5
Size: 47 5685 2081
Fragments arranged by size:
5685 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7813
StyI C'CwwG_G
Cuts at: 513 1065 2533 4462 4780 5097 5252 513
Size: 552 1468 1929 318 317 155 3074
Fragments arranged by size:
3074 1929 1468 552 318 317 155
SwaI ATTT'AAAT
Cuts at: 2341 2341
Size: 7813
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4014 5840 5857 6010 6195 6640 325
Size: 1255 2434 1826 17 153 185 445 1498
Fragments arranged by size:
2434 1826 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2400 4918 6059
Size: 274 80 33 51 1443 423 2518 1141
Cuts at: 6059 7771 96
Size: 1712 138
Fragments arranged by size:
2518 1712 1443 1141 423 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5007 5039 7773 7805 5007
Size: 32 2734 32 5015
Fragments arranged by size:
5015 2734 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7813
XbaI T'CTAG_A
Cuts at: 5149 5149
Size: 7813
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5095 5095
Size: 7813
XhoI C'TCGA_G
Cuts at: 1943 2475 1943
Size: 532 7281
XmaI C'CCGG_G
Cuts at: 1870 5138 1870
Size: 3268 4545
XmnI GAAnn'nnTTC
Cuts at: 5942 5942
Size: 7813
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5621 276
Size: 53 83 186 936 4087 2468
Fragments arranged by size:
4087 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoO109I FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI