Restriction map for
pFN21AB5623
(Circular) (Six-base) MAPSORT of: pFN21AB5623.seq Check: 4351 from: 1 to: 7642
pFN21AB5623 7642 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5452 278
Size: 53 83 186 936 3916 2468
Fragments arranged by size:
3916 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4985 1937
Size: 3048 4594
Acc65I G'GTAC_C
Cuts at: 3981 4963 3981
Size: 982 6660
AclI AA'CG_TT
Cuts at: 1563 2089 5769 6142 1563
Size: 526 3680 373 3063
Fragments arranged by size:
3680 3063 526 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3784 3864 5705 7089 7357 1632
Size: 2152 80 1841 1384 268 1917
Fragments arranged by size:
2152 1917 1841 1384 268 80
AflII C'TTAA_G
Cuts at: 820 1017 2950 3175 820
Size: 197 1933 225 5287
Fragments arranged by size:
5287 1933 225 197
AflIII A'CryG_T
Cuts at: 1203 2135 2584 6541 7518 1203
Size: 932 449 3957 977 1327
Fragments arranged by size:
3957 1327 977 932 449
AgeI A'CCGG_T
Cuts at: 6473 6473
Size: 7642
AhdI GACnn_n'nnGTC
Cuts at: 6371 6371
Size: 7642
AleI CACnn'nnGTG
Cuts at: 1166 2267 1166
Size: 1101 6541
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7642
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2435 2467 2435
Size: 32 7610
AlwNI CAG_nnn'CTG
Cuts at: 2287 4407 4601 5385 6957 2287
Size: 2120 194 784 1572 2972
Fragments arranged by size:
2972 2120 1572 784 194
ApaLI G'TGCA_C
Cuts at: 3068 5699 6855 3068
Size: 2631 1156 3855
Fragments arranged by size:
3855 2631 1156
ApoI r'AATT_y
Cuts at: 1493 2383 3804 4364 4951 5177 5329 7438 1493
Size: 890 1421 560 587 226 152 2109 1697
Fragments arranged by size:
2109 1697 1421 890 587 560 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4358 6196 160
Size: 872 3326 1838 1606
Fragments arranged by size:
3326 1838 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7642
AvaI C'yCGr_G
Cuts at: 1870 1943 4967 1870
Size: 73 3024 4545
Fragments arranged by size:
4545 3024 73
AvrII C'CTAG_G
Cuts at: 4465 4465
Size: 7642
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2091 2124 3965 3998 2091
Size: 33 1841 33 5735
Fragments arranged by size:
5735 1841 33 33
BamHI G'GATC_C
Cuts at: 2776 4972 5339 2776
Size: 2196 367 5079
Fragments arranged by size:
5079 2196 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2486 3981 4819 4963 6418
Size: 325 217 627 699 1495 838 144 1455
Cuts at: 6418 618
Size: 1842
Fragments arranged by size:
1842 1495 1455 838 699 627 325 217
144
BanII G_rGCy'C
Cuts at: 1391 2313 2724 3453 4961 1391
Size: 922 411 729 1508 4072
Fragments arranged by size:
4072 1508 922 729 411
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6671
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5833 5867 1903
Size: 34 3896 34 3678
Fragments arranged by size:
3896 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2545 3851 3898 4421 4642 5534 6744 2545
Size: 1306 47 523 221 892 1210 3443
Fragments arranged by size:
3443 1306 1210 892 523 221 47
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7642
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6253 136
Size: 107 122 71 631 605 4581 1525
Fragments arranged by size:
4581 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7637 7637
Size: 7642
BlpI GC'TnA_GC
Cuts at: 5059 5059
Size: 7642
Bme1580I G_kGCm'C
Cuts at: 1790 3072 5703 6859 1790
Size: 1282 2631 1156 2573
Fragments arranged by size:
2631 2573 1282 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7642
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4170 4756 4805
Size: 416 53 83 399 24 2753 586 49
Cuts at: 4805 5373 6331 442
Size: 568 958 1753
Fragments arranged by size:
2753 1753 958 586 568 416 399 83
53 49 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7642
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2410 2419 3334 3678 4755 6302 1239
Size: 1171 9 915 344 1077 1547 2579
Fragments arranged by size:
2579 1547 1171 1077 915 344 9
Bpu10I CC'TnA_GC
Cuts at: 2376 2376
Size: 7642
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2077 2735 2886 5767 6632 6930 2077
Size: 658 151 2881 865 298 2789
Fragments arranged by size:
2881 2789 865 658 298 151
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4301 6305 882
Size: 617 2802 2004 2219
Fragments arranged by size:
2802 2219 2004 617
BsaAI yAC'GTr
Cuts at: 493 1204 3895 5433 493
Size: 711 2691 1538 2702
Fragments arranged by size:
2702 2691 1538 711
BsaBI GATnn'nnATC
Cuts at: 2213 4232 7636 2213
Size: 2019 3404 2219
Fragments arranged by size:
3404 2219 2019
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5449 5831 6466 275
Size: 53 83 186 936 3916 382 635 1451
Fragments arranged by size:
3916 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4204 6075 6473 6747 6894 7305 4204
Size: 1871 398 274 147 411 4541
Fragments arranged by size:
4541 1871 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4697 4727 1178
Size: 30 165 30 3294 30 4093
Fragments arranged by size:
4093 3294 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2732 4207 4894 1186
Size: 1546 1475 687 3934
Fragments arranged by size:
3934 1546 1475 687
BseYI C'CCAG_C
Cuts at: 2298 4654 4729 6845 7341 2298
Size: 2356 75 2116 496 2599
Fragments arranged by size:
2599 2356 2116 496 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7069
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4887 5092 5853 6881 1540
Size: 275 186 2886 205 761 1028 2301
Fragments arranged by size:
2886 2301 1028 761 275 205 186
BsiHKAI G_wGCw'C
Cuts at: 2313 3072 3453 3711 5703 5788 6859 2313
Size: 759 381 258 1992 85 1071 3096
Fragments arranged by size:
3096 1992 1071 759 381 258 85
BsmI GAATG_Cn'
Cuts at: 2594 5153 5246 2594
Size: 2559 93 4990
Fragments arranged by size:
4990 2559 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2313 2724 3072 3453 3711 4961 5703
Size: 399 523 411 348 381 258 1250 742
Cuts at: 5703 5788 6859 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1250 1071 742 523 411 399 381
348 258 85
BspEI T'CCGG_A
Cuts at: 4204 7305 4204
Size: 3101 4541
BspHI T'CATG_A
Cuts at: 3054 5531 7261 3054
Size: 2477 1730 3435
Fragments arranged by size:
3435 2477 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3199 4997 844
Size: 2355 1798 3489
Fragments arranged by size:
3489 2355 1798
BsrBI CCG'CTC
Cuts at: 1394 1823 5529 1394
Size: 429 3706 3507
Fragments arranged by size:
3706 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2045 6137 6311 65
Size: 1183 797 4092 174 1396
Fragments arranged by size:
4092 1396 1183 797 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6286 6473 1700
Size: 110 4476 187 2869
Fragments arranged by size:
4476 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2422 2867 96
Size: 2326 445 4871
Fragments arranged by size:
4871 2326 445
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7642
BssSI C'ACGA_G
Cuts at: 5702 6714 5702
Size: 1012 6630
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7642
BstBI TT'CG_AA
Cuts at: 6446 6446
Size: 7642
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7642
BstXI CCAn_nnnn'nTGG
Cuts at: 2388 2388
Size: 7642
BstYI r'GATC_y
Cuts at: 1972 2208 2776 3396 4921 4972 5339 5724 5741
Size: 236 568 620 1525 51 367 385 17
Cuts at: 5741 7193 7279 7291 7637 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1525 1452 620 568 385 367 346
236 86 51 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3115 4245 513
Size: 552 939 1111 1130 3910
Fragments arranged by size:
3910 1130 1111 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6903
Fragments arranged by size:
6903 683 56
BtsI GCAGTG_nn'
Cuts at: 2790 4019 5158 5952 5972 2790
Size: 1229 1139 794 20 4460
Fragments arranged by size:
4460 1229 1139 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7642
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2317 2352 529
Size: 35 1753 35 5819
Fragments arranged by size:
5819 1753 35 35
DraI TTT'AAA
Cuts at: 4946 4946
Size: 7642
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6649 809
Size: 507 5333 1802
Fragments arranged by size:
5333 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4618 5089 5978
Size: 54 1425 50 275 18 2788 471 889
Cuts at: 5978 7607 8
Size: 1629 43
Fragments arranged by size:
2788 1629 1425 889 471 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5089 1537
Size: 275 3277 4090
Fragments arranged by size:
4090 3277 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2951 5572 1353
Size: 1598 2621 3423
Fragments arranged by size:
3423 2621 1598
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6229 6603 6749 387
Size: 1064 138 4640 374 146 1280
Fragments arranged by size:
4640 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2311 3451 2311
Size: 1140 6502
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2410 2419 3334 3678 3784 3864 4755
Size: 393 778 9 915 344 106 80 891
Cuts at: 4755 5705 6302 7089 7357 1239
Size: 950 597 787 268 1524
Fragments arranged by size:
1524 950 915 891 787 778 597 393
344 268 106 80 9
EcoO109I rG'GnC_Cy
Cuts at: 3301 3737 4172 3301
Size: 436 435 6771
Fragments arranged by size:
6771 436 435
EcoRI G'AATT_C
Cuts at: 4951 5329 4951
Size: 378 7264
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2847 3849 2847
Size: 1002 6640
FspI TGC'GCA
Cuts at: 6148 6148
Size: 7642
HaeII r_GCGC'y
Cuts at: 1678 6789 1678
Size: 5111 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3422 3454 4199 4231 4300
Size: 32 135 32 1850 32 745 32 69
Cuts at: 4300 4332 4595 4627 6283 6315 6357 6389 1373
Size: 32 263 32 1656 32 42 32 2626
Fragments arranged by size:
2626 1850 1656 745 263 135 69 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3022 4500 4986 5232 669
Size: 988 281 1084 1478 486 246 3079
Fragments arranged by size:
3079 1478 1084 988 486 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7642
HpaI GTT'AAC
Cuts at: 5232 5232
Size: 7642
KpnI G_GTAC'C
Cuts at: 3985 4967 3985
Size: 982 6660
MfeI C'AATT_G
Cuts at: 5241 5241
Size: 7642
MluI A'CGCG_T
Cuts at: 2135 7518 2135
Size: 5383 2259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3917 4553 6756 6940 1305
Size: 2612 636 2203 184 2007
Fragments arranged by size:
2612 2203 2007 636 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4620 10
Size: 54 1425 343 2788 3032
Fragments arranged by size:
3032 2788 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2246 2267 3097 3155 3387 4274 5600
Size: 648 1080 21 830 58 232 887 1326
Cuts at: 5600 5959 6118 518
Size: 359 159 2042
Fragments arranged by size:
2042 1326 1080 887 830 648 359 232
159 58 21
MspA1I CmG'CkG
Cuts at: 1606 4733 5058 5735 6883 7128 1606
Size: 3127 325 677 1148 245 2120
Fragments arranged by size:
3127 2120 1148 677 325 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3115 4245 513
Size: 552 939 1111 1130 3910
Fragments arranged by size:
3910 1130 1111 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7642
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7642
NotI GC'GGCC_GC
Cuts at: 5089 5089
Size: 7642
NspI r_CATG'y
Cuts at: 1132 1229 2588 3386 5000 6545 1132
Size: 97 1359 798 1614 1545 2229
Fragments arranged by size:
2229 1614 1545 1359 798 97
PasI CC'CwG_GG
Cuts at: 1348 4717 1348
Size: 3369 4273
PciI A'CATG_T
Cuts at: 2584 6541 2584
Size: 3957 3685
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7642
PmeI GTTT'AAAC
Cuts at: 4946 4946
Size: 7642
PmlI CAC'GTG
Cuts at: 3895 3895
Size: 7642
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5711 5743 7224 7256 5711
Size: 32 1481 32 6097
Fragments arranged by size:
6097 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3737 3737
Size: 7642
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7642
PsiI TTA'TAA
Cuts at: 5212 5212
Size: 7642
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3152 3184 3152
Size: 32 7610
PstI C_TGCA'G
Cuts at: 830 3577 4994 830
Size: 2747 1417 3478
Fragments arranged by size:
3478 2747 1417
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7642
PvuII CAG'CTG
Cuts at: 4733 4733
Size: 7642
SacI G_AGCT'C
Cuts at: 2313 3453 2313
Size: 1140 6502
SalI G'TCGA_C
Cuts at: 1936 4984 1936
Size: 3048 4594
SbfI CC_TGCA'GG
Cuts at: 4994 4994
Size: 7642
ScaI AGT'ACT
Cuts at: 2712 3170 5890 7602 2712
Size: 458 2720 1712 2752
Fragments arranged by size:
2752 2720 1712 458
SexAI A'CCwGG_T
Cuts at: 2280 3964 2280
Size: 1684 5958
SfcI C'TryA_G
Cuts at: 826 1045 3573 4990 6125 6806 6997 826
Size: 219 2528 1417 1135 681 191 1471
Fragments arranged by size:
2528 1471 1417 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7642
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7642
SmaI CCC'GGG
Cuts at: 1872 4969 1872
Size: 3097 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2056 2714 2901 2950 3175 5746
Size: 197 926 113 658 187 49 225 2571
Cuts at: 5746 6647 6909 820
Size: 901 262 1553
Fragments arranged by size:
2571 1553 926 901 658 262 225 197
187 113 49
SnaBI TAC'GTA
Cuts at: 493 5433 493
Size: 4940 2702
SpeI A'CTAG_T
Cuts at: 152 4157 152
Size: 4005 3637
SphI G_CATG'C
Cuts at: 1132 3386 5000 1132
Size: 2254 1614 3774
Fragments arranged by size:
3774 2254 1614
SspI AAT'ATT
Cuts at: 5 52 5566 5
Size: 47 5514 2081
Fragments arranged by size:
5514 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7642
StyI C'CwwG_G
Cuts at: 513 1065 2004 2724 2796 3115 4002 4245 4465
Size: 552 939 720 72 319 887 243 220
Cuts at: 4465 5081 513
Size: 616 3074
Fragments arranged by size:
3074 939 887 720 616 552 319 243
220 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2236 3530 4035 4789 4901 5669 5686
Size: 1255 656 1294 505 754 112 768 17
Cuts at: 5686 5839 6024 6469 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1294 1255 768 754 656 505 445
185 153 112 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2422 2710 2768
Size: 274 80 33 51 1443 445 288 58
Cuts at: 2768 2867 3168 3201 4681 5888 7600 96
Size: 99 301 33 1480 1207 1712 138
Fragments arranged by size:
1712 1480 1443 1207 445 301 288 274
138 99 80 58 51 33 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2947 2979 3235 3267 7602 7634 2947
Size: 32 256 32 4335 32 2955
Fragments arranged by size:
4335 2955 256 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7642
XbaI T'CTAG_A
Cuts at: 4978 4978
Size: 7642
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3202 3535 3202
Size: 333 7309
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7642
XmaI C'CCGG_G
Cuts at: 1870 4967 1870
Size: 3097 4545
XmnI GAAnn'nnTTC
Cuts at: 3086 5771 3086
Size: 2685 4957
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5450 276
Size: 53 83 186 936 3916 2468
Fragments arranged by size:
3916 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PspOMI PspXI RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI