Restriction map for
pFN21AB5607
(Circular) (Six-base) MAPSORT of: pFN21AB5607.seq Check: 5875 from: 1 to: 6100
pFN21AB5607 6100 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2414 2840 2414
Size: 426 5674
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3910 278
Size: 53 83 186 936 2374 2468
Fragments arranged by size:
2468 2374 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2535 2613 3443 1937
Size: 598 78 830 4594
Fragments arranged by size:
4594 830 598 78
Acc65I G'GTAC_C
Cuts at: 2651 3421 2651
Size: 770 5330
AclI AA'CG_TT
Cuts at: 1563 4227 4600 1563
Size: 2664 373 3063
Fragments arranged by size:
3063 2664 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4163 5547 5815 1632
Size: 2531 1384 268 1917
Fragments arranged by size:
2531 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5903
AflIII A'CryG_T
Cuts at: 1203 2258 4999 5976 1203
Size: 1055 2741 977 1327
Fragments arranged by size:
2741 1327 1055 977
AgeI A'CCGG_T
Cuts at: 4931 4931
Size: 6100
AhdI GACnn_n'nnGTC
Cuts at: 3372 4829 3372
Size: 1457 4643
AleI CACnn'nnGTG
Cuts at: 1166 2571 2663 1166
Size: 1405 92 4603
Fragments arranged by size:
4603 1405 92
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6100
AlwNI CAG_nnn'CTG
Cuts at: 2090 2999 3053 3843 5415 2090
Size: 909 54 790 1572 2775
Fragments arranged by size:
2775 1572 909 790 54
ApaI G_GGCC'C
Cuts at: 2681 2681
Size: 6100
ApaLI G'TGCA_C
Cuts at: 4157 5313 4157
Size: 1156 4944
ApoI r'AATT_y
Cuts at: 1493 3409 3635 3787 5896 1493
Size: 1916 226 152 2109 1697
Fragments arranged by size:
2109 1916 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4654 160
Size: 872 3622 1606
Fragments arranged by size:
3622 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
AvaI C'yCGr_G
Cuts at: 1870 1943 2607 2913 3314 3425 1870
Size: 73 664 306 401 111 4545
Fragments arranged by size:
4545 664 401 306 111 73
BamHI G'GATC_C
Cuts at: 3430 3797 3430
Size: 367 5733
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2651 2858 3421 4876 618
Size: 325 217 627 864 207 563 1455 1842
Fragments arranged by size:
1842 1455 864 627 563 325 217 207
BanII G_rGCy'C
Cuts at: 1391 2035 2271 2681 3219 3419 1391
Size: 644 236 410 538 200 4072
Fragments arranged by size:
4072 644 538 410 236 200
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5129
BbvCI CC'TCA_GC
Cuts at: 2839 2839
Size: 6100
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2702 2736 4291 4325 1903
Size: 34 765 34 1555 34 3678
Fragments arranged by size:
3678 1555 765 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3992 5202 3992
Size: 1210 4890
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6100
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4711 136
Size: 107 122 71 631 605 3039 1525
Fragments arranged by size:
3039 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3084 6095 3084
Size: 3011 3089
BlpI GC'TnA_GC
Cuts at: 3517 3517
Size: 6100
Bme1580I G_kGCm'C
Cuts at: 1790 2258 2681 4161 5317 1790
Size: 468 423 1480 1156 2573
Fragments arranged by size:
2573 1480 1156 468 423
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6100
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2626 3831 4789
Size: 416 53 83 399 24 1209 1205 958
Cuts at: 4789 442
Size: 1753
Fragments arranged by size:
1753 1209 1205 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6100
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2051 2707 2051
Size: 656 5444
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4760 1239
Size: 3521 2579
Bpu10I CC'TnA_GC
Cuts at: 2682 2839 3218 2682
Size: 157 379 5564
Fragments arranged by size:
5564 379 157
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4225 5090 5388 4225
Size: 865 298 4937
Fragments arranged by size:
4937 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3008 3282 4763 882
Size: 617 1509 274 1481 2219
Fragments arranged by size:
2219 1509 1481 617 274
BsaAI yAC'GTr
Cuts at: 493 1204 2573 3891 493
Size: 711 1369 1318 2702
Fragments arranged by size:
2702 1369 1318 711
BsaBI GATnn'nnATC
Cuts at: 6094 6094
Size: 6100
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3907 4289 4924 275
Size: 53 83 186 936 2374 382 635 1451
Fragments arranged by size:
2374 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2101 4533 4931 5205 5352 5763 2101
Size: 2432 398 274 147 411 2438
Fragments arranged by size:
2438 2432 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2497 2527 2700 2730 3018
Size: 30 165 30 1094 30 173 30 288
Cuts at: 3018 3048 1178
Size: 30 4230
Fragments arranged by size:
4230 1094 288 173 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2043 2147 3026 3227 1186
Size: 857 104 879 201 4059
Fragments arranged by size:
4059 879 857 201 104
BseYI C'CCAG_C
Cuts at: 3369 5303 5799 3369
Size: 1934 496 3670
Fragments arranged by size:
3670 1934 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2392 2818 1163
Size: 573 656 426 4445
Fragments arranged by size:
4445 656 573 426
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3550 4311 5339 1540
Size: 275 186 1549 761 1028 2301
Fragments arranged by size:
2301 1549 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2035 2271 2965 3219 4161 4246 5317 2035
Size: 236 694 254 942 85 1071 2818
Fragments arranged by size:
2818 1071 942 694 254 236 85
BsmI GAATG_Cn'
Cuts at: 3611 3704 3611
Size: 93 6007
BsmBI CGTCTCn'nnnn_
Cuts at: 2782 2782
Size: 6100
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2258 2271 2681 2965 3219 3419
Size: 399 245 223 13 410 284 254 200
Cuts at: 3419 4161 4246 5317 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 410 399 284 254 245
223 200 85 13
BspEI T'CCGG_A
Cuts at: 2101 5763 2101
Size: 3662 2438
BspHI T'CATG_A
Cuts at: 3989 5719 3989
Size: 1730 4370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2075 2414 2840 3455 844
Size: 1231 339 426 615 3489
Fragments arranged by size:
3489 1231 615 426 339
BsrBI CCG'CTC
Cuts at: 1394 1823 3987 1394
Size: 429 2164 3507
Fragments arranged by size:
3507 2164 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2321 4595 4769 65
Size: 1183 1073 2274 174 1396
Fragments arranged by size:
2274 1396 1183 1073 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4744 4931 1700
Size: 110 2934 187 2869
Fragments arranged by size:
2934 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6100
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6100
BssSI C'ACGA_G
Cuts at: 4160 5172 4160
Size: 1012 5088
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2320 794
Size: 1526 4574
BstBI TT'CG_AA
Cuts at: 4904 4904
Size: 6100
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6100
BstXI CCAn_nnnn'nTGG
Cuts at: 2882 2882
Size: 6100
BstYI r'GATC_y
Cuts at: 1972 2542 2553 3084 3430 3797 4182 4199 5651
Size: 570 11 531 346 367 385 17 1452
Cuts at: 5651 5737 5749 6095 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 570 531 385 367 346 346
86 17 12 11
BstZ17I GTA'TAC
Cuts at: 2614 2614
Size: 6100
Bsu36I CC'TnA_GG
Cuts at: 3162 3162
Size: 6100
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4609
Fragments arranged by size:
4609 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5361
Fragments arranged by size:
5361 683 56
BtsI GCAGTG_nn'
Cuts at: 2070 2727 3616 4410 4430 2070
Size: 657 889 794 20 3740
Fragments arranged by size:
3740 889 794 657 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6100
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6065
DraI TTT'AAA
Cuts at: 3404 3404
Size: 6100
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2165 5107 809
Size: 507 849 2942 1802
Fragments arranged by size:
2942 1802 849 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3547 4436
Size: 54 1425 50 275 18 177 1540 889
Cuts at: 4436 6065 8
Size: 1629 43
Fragments arranged by size:
1629 1540 1425 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3547 1537
Size: 275 1735 4090
Fragments arranged by size:
4090 1735 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4030 1353
Size: 2677 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4687 5061 5207 387
Size: 1064 138 3098 374 146 1280
Fragments arranged by size:
3098 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2033 2269 3217 2033
Size: 236 948 4916
Fragments arranged by size:
4916 948 236
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4163 4760 5547 5815 1239
Size: 393 2531 597 787 268 1524
Fragments arranged by size:
2531 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2353 2353
Size: 6100
EcoO109I rG'GnC_Cy
Cuts at: 2137 2620 2677 2678 2137
Size: 483 57 1 5559
Fragments arranged by size:
5559 483 57 1
EcoRI G'AATT_C
Cuts at: 3409 3787 3409
Size: 378 5722
FspI TGC'GCA
Cuts at: 4606 4606
Size: 6100
HaeII r_GCGC'y
Cuts at: 1678 3235 5247 1678
Size: 1557 2012 2531
Fragments arranged by size:
2531 2012 1557
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2545 2577 3024 3056 3358
Size: 32 135 32 973 32 447 32 302
Cuts at: 3358 3390 4741 4773 4815 4847 1373
Size: 32 1351 32 42 32 2626
Fragments arranged by size:
2626 1351 973 447 302 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2983 2989 3366 3444 3690 669
Size: 988 281 1045 6 377 78 246 3079
Fragments arranged by size:
3079 1045 988 377 281 246 78 6
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6100
HpaI GTT'AAC
Cuts at: 3690 3690
Size: 6100
KpnI G_GTAC'C
Cuts at: 2655 3425 2655
Size: 770 5330
MfeI C'AATT_G
Cuts at: 3699 3699
Size: 6100
MluI A'CGCG_T
Cuts at: 2258 5976 2258
Size: 3718 2382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3087 5214 5398 1305
Size: 1782 2127 184 2007
Fragments arranged by size:
2127 2007 1782 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 4101
Fragments arranged by size:
4101 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2074 2571 2663 2880 4058 4417 4576
Size: 648 908 497 92 217 1178 359 159
Cuts at: 4576 518
Size: 2042
Fragments arranged by size:
2042 1178 908 648 497 359 217 159
92
MspA1I CmG'CkG
Cuts at: 1606 2448 2559 2760 2843 2996 3193 3516 4193
Size: 842 111 201 83 153 197 323 677
Cuts at: 4193 5341 5586 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 842 677 323 245 201 197
153 111 83
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4609
Fragments arranged by size:
4609 939 552
NdeI CA'TA_TG
Cuts at: 387 3392 387
Size: 3005 3095
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6100
NotI GC'GGCC_GC
Cuts at: 3547 3547
Size: 6100
NspI r_CATG'y
Cuts at: 1132 1229 2217 2717 3131 3458 5003 1132
Size: 97 988 500 414 327 1545 2229
Fragments arranged by size:
2229 1545 988 500 414 327 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6100
PciI A'CATG_T
Cuts at: 4999 4999
Size: 6100
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6100
PfoI T'CCnGG_A
Cuts at: 2196 3180 2196
Size: 984 5116
PmeI GTTT'AAAC
Cuts at: 3404 3404
Size: 6100
PmlI CAC'GTG
Cuts at: 2573 2573
Size: 6100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4169 4201 5682 5714 4169
Size: 32 1481 32 4555
Fragments arranged by size:
4555 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2137 2620 2137
Size: 483 5617
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6100
PsiI TTA'TAA
Cuts at: 3075 3670 3075
Size: 595 5505
PspOMI G'GGCC_C
Cuts at: 2677 2677
Size: 6100
PspXI vC'TCGA_Gb
Cuts at: 2607 2607
Size: 6100
PstI C_TGCA'G
Cuts at: 830 2357 3452 830
Size: 1527 1095 3478
Fragments arranged by size:
3478 1527 1095
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6100
PvuII CAG'CTG
Cuts at: 2448 2760 2996 3193 2448
Size: 312 236 197 5355
Fragments arranged by size:
5355 312 236 197
SacI G_AGCT'C
Cuts at: 2035 2271 3219 2035
Size: 236 948 4916
Fragments arranged by size:
4916 948 236
SalI G'TCGA_C
Cuts at: 1936 3442 1936
Size: 1506 4594
SanDI GG'GwC_CC
Cuts at: 2620 2620
Size: 6100
SbfI CC_TGCA'GG
Cuts at: 3452 3452
Size: 6100
ScaI AGT'ACT
Cuts at: 4348 6060 4348
Size: 1712 4388
SfcI C'TryA_G
Cuts at: 826 1045 2353 2443 3095 3448 4583 5264 5455
Size: 219 1308 90 652 353 1135 681 191
Cuts at: 5455 826
Size: 1471
Fragments arranged by size:
1471 1308 1135 681 652 353 219 191
90
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6100
SmaI CCC'GGG
Cuts at: 1872 2915 3427 1872
Size: 1043 512 4545
Fragments arranged by size:
4545 1043 512
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2607 4204 5105 5367 820
Size: 197 926 664 1597 901 262 1553
Fragments arranged by size:
1597 1553 926 901 664 262 197
SnaBI TAC'GTA
Cuts at: 493 3891 493
Size: 3398 2702
SpeI A'CTAG_T
Cuts at: 152 2390 152
Size: 2238 3862
SphI G_CATG'C
Cuts at: 1132 3458 1132
Size: 2326 3774
SspI AAT'ATT
Cuts at: 5 52 4024 5
Size: 47 3972 2081
Fragments arranged by size:
3972 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6100
StyI C'CwwG_G
Cuts at: 513 1065 2004 3539 513
Size: 552 939 1535 3074
Fragments arranged by size:
3074 1535 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2754 3300 4127 4144 4297 4482 4927
Size: 1255 1174 546 827 17 153 185 445
Cuts at: 4927 325
Size: 1498
Fragments arranged by size:
1498 1255 1174 827 546 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2779 4346 6058
Size: 274 80 33 51 1443 802 1567 1712
Cuts at: 6058 96
Size: 138
Fragments arranged by size:
1712 1567 1443 802 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2497 2529 2700 2732 6060 6092 2497
Size: 32 171 32 3328 32 2505
Fragments arranged by size:
3328 2505 171 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6100
XbaI T'CTAG_A
Cuts at: 3436 3436
Size: 6100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2331 3112 3142 2331
Size: 781 30 5289
Fragments arranged by size:
5289 781 30
XhoI C'TCGA_G
Cuts at: 1943 2607 1943
Size: 664 5436
XmaI C'CCGG_G
Cuts at: 1870 2913 3425 1870
Size: 1043 512 4545
Fragments arranged by size:
4545 1043 512
XmnI GAAnn'nnTTC
Cuts at: 4229 4229
Size: 6100
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3908 276
Size: 53 83 186 936 2374 2468
Fragments arranged by size:
2468 2374 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
DraIII EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PsrI RsrII SacII SapI
SexAI SfiI SfoI SrfI SwaI