Restriction map for
pFN21AB5605
(Circular) (Six-base) MAPSORT of: pFN21AB5605.seq Check: 2956 from: 1 to: 6430
pFN21AB5605 6430 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2355 2534 3627 2355
Size: 179 1093 5158
Fragments arranged by size:
5158 1093 179
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4240 278
Size: 53 83 186 936 2704 2468
Fragments arranged by size:
2704 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3773 1937
Size: 1836 4594
Acc65I G'GTAC_C
Cuts at: 3383 3751 3383
Size: 368 6062
AclI AA'CG_TT
Cuts at: 1563 4557 4930 1563
Size: 2994 373 3063
Fragments arranged by size:
3063 2994 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3097 3309 4493 5877 6145 1632
Size: 1465 212 1184 1384 268 1917
Fragments arranged by size:
1917 1465 1384 1184 268 212
AfeI AGC'GCT
Cuts at: 3020 3020
Size: 6430
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6233
AflIII A'CryG_T
Cuts at: 1203 2766 5329 6306 1203
Size: 1563 2563 977 1327
Fragments arranged by size:
2563 1563 1327 977
AgeI A'CCGG_T
Cuts at: 2967 5261 2967
Size: 2294 4136
AhdI GACnn_n'nnGTC
Cuts at: 3240 5159 3240
Size: 1919 4511
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6430
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6430
AlwNI CAG_nnn'CTG
Cuts at: 2623 3145 4173 5745 2623
Size: 522 1028 1572 3308
Fragments arranged by size:
3308 1572 1028 522
ApaI G_GGCC'C
Cuts at: 2129 2316 3408 2129
Size: 187 1092 5151
Fragments arranged by size:
5151 1092 187
ApaLI G'TGCA_C
Cuts at: 2674 4487 5643 2674
Size: 1813 1156 3461
Fragments arranged by size:
3461 1813 1156
ApoI r'AATT_y
Cuts at: 1493 3739 3965 4117 6226 1493
Size: 2246 226 152 2109 1697
Fragments arranged by size:
2246 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4984 160
Size: 872 3952 1606
Fragments arranged by size:
3952 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
AvaI C'yCGr_G
Cuts at: 1870 1943 2122 2309 3755 1870
Size: 73 179 187 1446 4545
Fragments arranged by size:
4545 1446 187 179 73
BamHI G'GATC_C
Cuts at: 3760 4127 3760
Size: 367 6063
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2256 2552 2585 2719 2813
Size: 325 217 627 469 296 33 134 94
Cuts at: 2813 3213 3383 3434 3635 3751 5206 618
Size: 400 170 51 201 116 1455 1842
Fragments arranged by size:
1842 1455 627 469 400 325 296 217
201 170 134 116 94 51 33
BanII G_rGCy'C
Cuts at: 1391 2129 2316 2395 2550 2791 2918 3003 3099
Size: 738 187 79 155 241 127 85 96
Cuts at: 3099 3408 3489 3540 3749 1391
Size: 309 81 51 209 4072
Fragments arranged by size:
4072 738 309 241 209 187 155 127
96 85 81 79 51
BbeI G_GCGC'C
Cuts at: 2260 2556 2723 2817 3639 2260
Size: 296 167 94 822 5051
Fragments arranged by size:
5051 822 296 167 94
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5459
BbvCI CC'TCA_GC
Cuts at: 2433 2433
Size: 6430
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2575 2609 3310 3344 3553
Size: 34 50 34 554 34 701 34 209
Cuts at: 3553 3587 4621 4655 1903
Size: 34 1034 34 3678
Fragments arranged by size:
3678 1034 701 554 209 50 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4322 5532 4322
Size: 1210 5220
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6430
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2648 2717 2811
Size: 107 122 71 631 605 976 69 94
Cuts at: 2811 2825 3291 3432 3644 5041 136
Size: 14 466 141 212 1397 1525
Fragments arranged by size:
1525 1397 976 631 605 466 212 141
122 107 94 71 69 14
BglII A'GATC_T
Cuts at: 2154 6425 2154
Size: 4271 2159
BlpI GC'TnA_GC
Cuts at: 3847 3847
Size: 6430
Bme1580I G_kGCm'C
Cuts at: 1790 2129 2316 2374 2448 2678 2706 2807 3287
Size: 339 187 58 74 230 28 101 480
Cuts at: 3287 3408 4491 5647 1790
Size: 121 1083 1156 2573
Fragments arranged by size:
2573 1156 1083 480 339 230 187 121
101 74 58 28
BmgBI CAC'GTC
Cuts at: 1318 2980 1318
Size: 1662 4768
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2716 2997 4161
Size: 416 53 83 399 24 1299 281 1164
Cuts at: 4161 5119 442
Size: 958 1753
Fragments arranged by size:
1753 1299 1164 958 416 399 281 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3276 1056
Size: 2220 4210
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3017 5090 1239
Size: 1778 2073 2579
Fragments arranged by size:
2579 2073 1778
Bpu10I CC'TnA_GC
Cuts at: 2433 2433
Size: 6430
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4555 5420 5718 4555
Size: 865 298 5267
Fragments arranged by size:
5267 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2930 5093 882
Size: 617 1431 2163 2219
Fragments arranged by size:
2219 2163 1431 617
BsaAI yAC'GTr
Cuts at: 493 1204 3700 4221 493
Size: 711 2496 521 2702
Fragments arranged by size:
2702 2496 711 521
BsaBI GATnn'nnATC
Cuts at: 6424 6424
Size: 6430
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2257 2553 2720 2814
Size: 53 83 186 936 724 296 167 94
Cuts at: 2814 3503 3636 3716 4237 4619 5254 275
Size: 689 133 80 521 382 635 1451
Fragments arranged by size:
1451 936 724 689 635 521 382 296
186 167 133 94 83 80 53
BsaWI w'CCGG_w
Cuts at: 2967 4863 5261 5535 5682 6093 2967
Size: 1896 398 274 147 411 3304
Fragments arranged by size:
3304 1896 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2459 2489 2822 2852 3431
Size: 30 165 30 1056 30 333 30 579
Cuts at: 3431 3461 3505 3535 3647 3677 1178
Size: 30 44 30 112 30 3931
Fragments arranged by size:
3931 1056 579 333 165 112 44 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2185 2480 3513 3599 1186
Size: 999 295 1033 86 4017
Fragments arranged by size:
4017 1033 999 295 86
BseYI C'CCAG_C
Cuts at: 2447 2518 2806 3494 5633 6129 2447
Size: 71 288 688 2139 496 2748
Fragments arranged by size:
2748 2139 688 496 288 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2827 3304 1163
Size: 573 1091 477 4289
Fragments arranged by size:
4289 1091 573 477
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2958 3531 3880 4641 5669 1540
Size: 275 186 957 573 349 761 1028 2301
Fragments arranged by size:
2301 1028 957 761 573 349 275 186
BsiHKAI G_wGCw'C
Cuts at: 2103 2678 2766 2886 4491 4576 5647 2103
Size: 575 88 120 1605 85 1071 2886
Fragments arranged by size:
2886 1605 1071 575 120 88 85
BsmI GAATG_Cn'
Cuts at: 3544 3941 4034 3544
Size: 397 93 5940
Fragments arranged by size:
5940 397 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2575 2575
Size: 6430
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 2129 2316 2374 2395 2448 2550
Size: 399 313 26 187 58 21 53 102
Cuts at: 2550 2678 2706 2766 2791 2807 2886 2918 3003
Size: 128 28 60 25 16 79 32 85
Cuts at: 3003 3099 3287 3408 3489 3540 3749 4491 4576
Size: 96 188 121 81 51 209 742 85
Cuts at: 4576 5647 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 313 209 188 187
128 121 102 96 85 85 81 79
60 58 53 51 32 28 26 25
21 16
BspEI T'CCGG_A
Cuts at: 6093 6093
Size: 6430
BspHI T'CATG_A
Cuts at: 4319 6049 4319
Size: 1730 4700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2355 2534 3627 3785 844
Size: 1511 179 1093 158 3489
Fragments arranged by size:
3489 1511 1093 179 158
BsrBI CCG'CTC
Cuts at: 1394 1823 2609 2693 3712 4317 1394
Size: 429 786 84 1019 605 3507
Fragments arranged by size:
3507 1019 786 605 429 84
BsrDI GCAATG_nn'
Cuts at: 65 1248 2779 4925 5099 65
Size: 1183 1531 2146 174 1396
Fragments arranged by size:
2146 1531 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2042 2253 2626 2810 2939 2946 2967
Size: 110 232 211 373 184 129 7 21
Cuts at: 2967 5074 5261 1700
Size: 2107 187 2869
Fragments arranged by size:
2869 2107 373 232 211 187 184 129
110 21 7
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6430
BssHII G'CGCG_C
Cuts at: 1925 2028 1925
Size: 103 6327
BssSI C'ACGA_G
Cuts at: 2923 3200 3539 4490 5502 2923
Size: 277 339 951 1012 3851
Fragments arranged by size:
3851 1012 951 339 277
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2091 3700 794
Size: 1297 1609 3524
Fragments arranged by size:
3524 1609 1297
BstBI TT'CG_AA
Cuts at: 5234 5234
Size: 6430
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6430
BstYI r'GATC_y
Cuts at: 1972 2154 3760 4127 4512 4529 5981 6067 6079
Size: 182 1606 367 385 17 1452 86 12
Cuts at: 6079 6425 1972
Size: 346 1977
Fragments arranged by size:
1977 1606 1452 385 367 346 182 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2556 2602 2643 3099 3531 513
Size: 552 1491 46 41 456 432 3412
Fragments arranged by size:
3412 1491 552 456 432 46 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3556 487
Size: 683 56 2330 3361
Fragments arranged by size:
3361 2330 683 56
BtsI GCAGTG_nn'
Cuts at: 2480 3052 3946 4740 4760 2480
Size: 572 894 794 20 4150
Fragments arranged by size:
4150 894 794 572 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6430
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6395
DraI TTT'AAA
Cuts at: 3734 3734
Size: 6430
DraIII CAC_nnn'GTG
Cuts at: 3038 3038
Size: 6430
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5437 809
Size: 507 4121 1802
Fragments arranged by size:
4121 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2248 2955
Size: 54 1425 50 275 18 210 208 707
Cuts at: 2955 3528 3877 4766 6395 8
Size: 573 349 889 1629 43
Fragments arranged by size:
1629 1425 889 707 573 349 275 210
208 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2955 3528 3877 1537
Size: 275 1143 573 349 4090
Fragments arranged by size:
4090 1143 573 349 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4360 1353
Size: 3007 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2629 5017 5391 5537 387
Size: 1064 138 1040 2388 374 146 1280
Fragments arranged by size:
2388 1280 1064 1040 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3017 3097 3309 4493 5090 5877 6145
Size: 393 1385 80 212 1184 597 787 268
Cuts at: 6145 1239
Size: 1524
Fragments arranged by size:
1524 1385 1184 787 597 393 268 212
80
EcoO109I rG'GnC_Cy
Cuts at: 2014 2126 2313 2014
Size: 112 187 6131
Fragments arranged by size:
6131 187 112
EcoRI G'AATT_C
Cuts at: 3739 4117 3739
Size: 378 6052
FseI GG_CCGG'CC
Cuts at: 2046 2046
Size: 6430
FspI TGC'GCA
Cuts at: 3623 4936 3623
Size: 1313 5117
HaeII r_GCGC'y
Cuts at: 1678 2260 2308 2556 2624 2723 2730 2817 2839
Size: 582 48 248 68 99 7 87 22
Cuts at: 2839 3022 3599 3608 3639 5577 1678
Size: 183 577 9 31 1938 2531
Fragments arranged by size:
2531 1938 582 577 248 183 99 87
68 48 31 22 9 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2822 2854 3226 3258 3431
Size: 32 135 32 1250 32 372 32 173
Cuts at: 3431 3461 3463 3493 5071 5103 5145 5177 1373
Size: 30 2 30 1578 32 42 32 2626
Fragments arranged by size:
2626 1578 1250 372 173 135 42 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3028 3774 4020 669
Size: 988 281 1090 746 246 3079
Fragments arranged by size:
3079 1090 988 746 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6430
HpaI GTT'AAC
Cuts at: 4020 4020
Size: 6430
KasI G'GCGC_C
Cuts at: 2256 2552 2719 2813 3635 2256
Size: 296 167 94 822 5051
Fragments arranged by size:
5051 822 296 167 94
KpnI G_GTAC'C
Cuts at: 3387 3755 3387
Size: 368 6062
MfeI C'AATT_G
Cuts at: 4029 4029
Size: 6430
MluI A'CGCG_T
Cuts at: 2766 6306 2766
Size: 3540 2890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5544 5728 1305
Size: 4239 184 2007
Fragments arranged by size:
4239 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4608
Fragments arranged by size:
4608 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4388 4747 4906 518
Size: 648 3222 359 159 2042
Fragments arranged by size:
3222 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2278 2429 2558 2604 2645 2796 3474 3846
Size: 672 151 129 46 41 151 678 372
Cuts at: 3846 4523 5671 5916 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 678 677 672 372 245 151
151 129 46 41
NaeI GCC'GGC
Cuts at: 2044 2255 2812 2044
Size: 211 557 5662
Fragments arranged by size:
5662 557 211
NarI GG'CG_CC
Cuts at: 2257 2553 2720 2814 3636 2257
Size: 296 167 94 822 5051
Fragments arranged by size:
5051 822 296 167 94
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5878
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6430
NgoMIV G'CCGG_C
Cuts at: 2042 2253 2810 2042
Size: 211 557 5662
Fragments arranged by size:
5662 557 211
NheI G'CTAG_C
Cuts at: 1052 3272 1052
Size: 2220 4210
NotI GC'GGCC_GC
Cuts at: 3877 3877
Size: 6430
NruI TCG'CGA
Cuts at: 3575 3575
Size: 6430
NspI r_CATG'y
Cuts at: 1132 1229 2932 3788 5333 1132
Size: 97 1703 856 1545 2229
Fragments arranged by size:
2229 1703 1545 856 97
PasI CC'CwG_GG
Cuts at: 1348 2783 1348
Size: 1435 4995
PciI A'CATG_T
Cuts at: 5329 5329
Size: 6430
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6430
PfoI T'CCnGG_A
Cuts at: 3300 3300
Size: 6430
PmeI GTTT'AAAC
Cuts at: 3734 3734
Size: 6430
PmlI CAC'GTG
Cuts at: 3700 3700
Size: 6430
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4499 4531 6012 6044 4499
Size: 32 1481 32 4885
Fragments arranged by size:
4885 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2014 2014
Size: 6430
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6430
PsiI TTA'TAA
Cuts at: 4000 4000
Size: 6430
PspOMI G'GGCC_C
Cuts at: 2125 2312 3404 2125
Size: 187 1092 5151
Fragments arranged by size:
5151 1092 187
PstI C_TGCA'G
Cuts at: 830 2277 2531 2741 2826 3696 3782 830
Size: 1447 254 210 85 870 86 3478
Fragments arranged by size:
3478 1447 870 254 210 86 85
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6430
PvuII CAG'CTG
Cuts at: 2278 2278
Size: 6430
RsrII CG'GwC_CG
Cuts at: 3705 3705
Size: 6430
SacII CC_GC'GG
Cuts at: 2559 2605 2646 2559
Size: 46 41 6343
Fragments arranged by size:
6343 46 41
SalI G'TCGA_C
Cuts at: 1936 3772 1936
Size: 1836 4594
SbfI CC_TGCA'GG
Cuts at: 2531 2826 3782 2531
Size: 295 956 5179
Fragments arranged by size:
5179 956 295
ScaI AGT'ACT
Cuts at: 4678 6390 4678
Size: 1712 4718
SexAI A'CCwGG_T
Cuts at: 3072 3072
Size: 6430
SfcI C'TryA_G
Cuts at: 826 1045 2273 2359 2527 2737 2822 3692 3778
Size: 219 1228 86 168 210 85 870 86
Cuts at: 3778 4913 5594 5785 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1228 1135 870 681 219 210 191
168 86 86 85
SfoI GGC'GCC
Cuts at: 2258 2554 2721 2815 3637 2258
Size: 296 167 94 822 5051
Fragments arranged by size:
5051 822 296 167 94
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
SgrAI Cr'CCGG_yG
Cuts at: 1700 2253 2967 1700
Size: 553 714 5163
Fragments arranged by size:
5163 714 553
SmaI CCC'GGG
Cuts at: 1872 2124 3757 1872
Size: 252 1633 4545
Fragments arranged by size:
4545 1633 252
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4534 5435 5697 820
Size: 197 926 2591 901 262 1553
Fragments arranged by size:
2591 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4221 493
Size: 3728 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6430
SphI G_CATG'C
Cuts at: 1132 3788 1132
Size: 2656 3774
SspI AAT'ATT
Cuts at: 5 52 4354 5
Size: 47 4302 2081
Fragments arranged by size:
4302 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6430
StyI C'CwwG_G
Cuts at: 513 1065 2023 3678 3869 513
Size: 552 958 1655 191 3074
Fragments arranged by size:
3074 1655 958 552 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3281 3722 4457 4474 4627 4812 5257
Size: 1255 1701 441 735 17 153 185 445
Cuts at: 5257 325
Size: 1498
Fragments arranged by size:
1701 1498 1255 735 445 441 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3657 4676 6388
Size: 274 80 33 51 1443 1680 1019 1712
Cuts at: 6388 96
Size: 138
Fragments arranged by size:
1712 1680 1443 1019 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2459 2491 3071 3103 3505 3537 3647 3679 6390
Size: 32 580 32 402 32 110 32 2711
Cuts at: 6390 6422 2459
Size: 32 2467
Fragments arranged by size:
2711 2467 580 402 110 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2860 1316
Size: 1544 4886
XbaI T'CTAG_A
Cuts at: 3766 3766
Size: 6430
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3649 3649
Size: 6430
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6430
XmaI C'CCGG_G
Cuts at: 1870 2122 3755 1870
Size: 252 1633 4545
Fragments arranged by size:
4545 1633 252
XmnI GAAnn'nnTTC
Cuts at: 4559 4559
Size: 6430
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4238 276
Size: 53 83 186 936 2704 2468
Fragments arranged by size:
2704 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BstXI
BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FspAI NsiI
PacI PspXI PsrI SacI SanDI SapI SfiI SrfI
SwaI