Restriction map for
pFN21AB5603
(Circular) (Six-base) MAPSORT of: pFN21AB5603.seq Check: 7616 from: 1 to: 6103
pFN21AB5603 6103 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3913 278
Size: 53 83 186 936 2377 2468
Fragments arranged by size:
2468 2377 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3446 1937
Size: 1509 4594
Acc65I G'GTAC_C
Cuts at: 3424 3424
Size: 6103
AclI AA'CG_TT
Cuts at: 1563 4230 4603 1563
Size: 2667 373 3063
Fragments arranged by size:
3063 2667 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2094 2541 4166 5550 5818 1632
Size: 462 447 1625 1384 268 1917
Fragments arranged by size:
1917 1625 1384 462 447 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5906
AflIII A'CryG_T
Cuts at: 1203 2359 2791 5002 5979 1203
Size: 1156 432 2211 977 1327
Fragments arranged by size:
2211 1327 1156 977 432
AgeI A'CCGG_T
Cuts at: 4934 4934
Size: 6103
AhdI GACnn_n'nnGTC
Cuts at: 4832 4832
Size: 6103
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6103
AlwNI CAG_nnn'CTG
Cuts at: 3846 5418 3846
Size: 1572 4531
ApaLI G'TGCA_C
Cuts at: 4160 5316 4160
Size: 1156 4947
ApoI r'AATT_y
Cuts at: 1493 2165 2864 3005 3412 3638 3790 5899 1493
Size: 672 699 141 407 226 152 2109 1697
Fragments arranged by size:
2109 1697 699 672 407 226 152 141
AseI AT'TA_AT
Cuts at: 160 1032 4657 160
Size: 872 3625 1606
Fragments arranged by size:
3625 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6103
AvaI C'yCGr_G
Cuts at: 1870 1943 2298 3428 1870
Size: 73 355 1130 4545
Fragments arranged by size:
4545 1130 355 73
BamHI G'GATC_C
Cuts at: 3433 3800 3433
Size: 367 5736
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3117 3424 4879 618
Size: 325 217 627 1330 307 1455 1842
Fragments arranged by size:
1842 1455 1330 627 325 307 217
BanII G_rGCy'C
Cuts at: 1391 3422 1391
Size: 2031 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2145 2440 2760 928
Size: 971 246 295 320 4271
Fragments arranged by size:
4271 971 320 295 246
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4294 4328 1903
Size: 34 2357 34 3678
Fragments arranged by size:
3678 2357 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3995 5205 3995
Size: 1210 4893
BclI T'GATC_A
Cuts at: 1548 2250 1548
Size: 702 5401
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4714 136
Size: 107 122 71 631 605 3042 1525
Fragments arranged by size:
3042 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3190 6098 3190
Size: 2908 3195
BlpI GC'TnA_GC
Cuts at: 3520 3520
Size: 6103
Bme1580I G_kGCm'C
Cuts at: 1790 4164 5320 1790
Size: 2374 1156 2573
Fragments arranged by size:
2573 2374 1156
BmgBI CAC'GTC
Cuts at: 1318 2256 1318
Size: 938 5165
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2767 3834 4792
Size: 416 53 83 399 24 1350 1067 958
Cuts at: 4792 442
Size: 1753
Fragments arranged by size:
1753 1350 1067 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2484 2691 3341 4763 1239
Size: 1245 207 650 1422 2579
Fragments arranged by size:
2579 1422 1245 650 207
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4228 5093 5391 4228
Size: 865 298 4940
Fragments arranged by size:
4940 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2620 4766 882
Size: 617 1121 2146 2219
Fragments arranged by size:
2219 2146 1121 617
BsaAI yAC'GTr
Cuts at: 493 1204 3894 493
Size: 711 2690 2702
Fragments arranged by size:
2702 2690 711
BsaBI GATnn'nnATC
Cuts at: 6097 6097
Size: 6103
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3910 4292 4927 275
Size: 53 83 186 936 2377 382 635 1451
Fragments arranged by size:
2377 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2860 4536 4934 5208 5355 5766 2860
Size: 1676 398 274 147 411 3197
Fragments arranged by size:
3197 1676 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5878
Fragments arranged by size:
5878 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2130 1186
Size: 944 5159
BseYI C'CCAG_C
Cuts at: 5306 5802 5306
Size: 496 5607
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5530
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3553 4314 5342 1540
Size: 275 186 1552 761 1028 2301
Fragments arranged by size:
2301 1552 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4164 4249 5320 4164
Size: 85 1071 4947
Fragments arranged by size:
4947 1071 85
BsmI GAATG_Cn'
Cuts at: 3614 3707 3614
Size: 93 6010
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3422 4164 4249 5320 1391
Size: 399 1632 742 85 1071 2174
Fragments arranged by size:
2174 1632 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5766 5766
Size: 6103
BspHI T'CATG_A
Cuts at: 3992 5722 3992
Size: 1730 4373
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2839 3458 844
Size: 1995 619 3489
Fragments arranged by size:
3489 1995 619
BsrBI CCG'CTC
Cuts at: 1394 1823 3990 1394
Size: 429 2167 3507
Fragments arranged by size:
3507 2167 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4598 4772 65
Size: 1183 3350 174 1396
Fragments arranged by size:
3350 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2141 3120 4747 4934 1700
Size: 110 331 979 1627 187 2869
Fragments arranged by size:
2869 1627 979 331 187 110
BsrGI T'GTAC_A
Cuts at: 96 3148 96
Size: 3052 3051
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6103
BssSI C'ACGA_G
Cuts at: 4163 5175 4163
Size: 1012 5091
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3388 794
Size: 2594 3509
BstBI TT'CG_AA
Cuts at: 4907 4907
Size: 6103
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6103
BstYI r'GATC_y
Cuts at: 1972 3190 3433 3800 4185 4202 5654 5740 5752
Size: 1218 243 367 385 17 1452 86 12
Cuts at: 5752 6098 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1218 385 367 346 243 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2187 2187
Size: 6103
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5551
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5364
Fragments arranged by size:
5364 683 56
BtsI GCAGTG_nn'
Cuts at: 2061 3619 4413 4433 2061
Size: 1558 794 20 3731
Fragments arranged by size:
3731 1558 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6103
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6068
DraI TTT'AAA
Cuts at: 3407 3407
Size: 6103
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2313 5110 809
Size: 507 997 2797 1802
Fragments arranged by size:
2797 1802 997 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3550 4439 6068
Size: 54 1425 50 275 18 1720 889 1629
Cuts at: 6068 8
Size: 43
Fragments arranged by size:
1720 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3550 1537
Size: 275 1738 4090
Fragments arranged by size:
4090 1738 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2187 2841 3181 4033 1353
Size: 834 654 340 852 3423
Fragments arranged by size:
3423 852 834 654 340
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4690 5064 5210 387
Size: 1064 138 3101 374 146 1280
Fragments arranged by size:
3101 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2094 2484 2541 2691 3341 4166 4763
Size: 393 462 390 57 150 650 825 597
Cuts at: 4763 5550 5818 1239
Size: 787 268 1524
Fragments arranged by size:
1524 825 787 650 597 462 393 390
268 150 57
EcoNI CCTnn'n_nnAGG
Cuts at: 2112 2112
Size: 6103
EcoRI G'AATT_C
Cuts at: 2864 3005 3412 3790 2864
Size: 141 407 378 5177
Fragments arranged by size:
5177 407 378 141
EcoRV GAT'ATC
Cuts at: 2295 2680 2295
Size: 385 5718
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2436 2436
Size: 6103
FspI TGC'GCA
Cuts at: 4609 4609
Size: 6103
HaeII r_GCGC'y
Cuts at: 1678 5250 1678
Size: 3572 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2188 2220 3345 3377 4744
Size: 32 135 32 616 32 1125 32 1367
Cuts at: 4744 4776 4818 4850 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1367 1125 616 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2259 3447 3693 669
Size: 988 281 321 1188 246 3079
Fragments arranged by size:
3079 1188 988 321 281 246
HindIII A'AGCT_T
Cuts at: 748 2025 3326 748
Size: 1277 1301 3525
Fragments arranged by size:
3525 1301 1277
HpaI GTT'AAC
Cuts at: 3693 3693
Size: 6103
KpnI G_GTAC'C
Cuts at: 3428 3428
Size: 6103
MfeI C'AATT_G
Cuts at: 3702 3702
Size: 6103
MluI A'CGCG_T
Cuts at: 5979 5979
Size: 6103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5217 5401 1305
Size: 3912 184 2007
Fragments arranged by size:
3912 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4281
Fragments arranged by size:
4281 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4061 4420 4579 518
Size: 648 2895 359 159 2042
Fragments arranged by size:
2895 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3519 4196 5344 5589 1606
Size: 1913 677 1148 245 2120
Fragments arranged by size:
2120 1913 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5551
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6103
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6103
NotI GC'GGCC_GC
Cuts at: 3550 3550
Size: 6103
NspI r_CATG'y
Cuts at: 1132 1229 2363 2795 3461 5006 1132
Size: 97 1134 432 666 1545 2229
Fragments arranged by size:
2229 1545 1134 666 432 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6103
PciI A'CATG_T
Cuts at: 2359 2791 5002 2359
Size: 432 2211 3460
Fragments arranged by size:
3460 2211 432
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2714 1108
Size: 1606 4497
PfoI T'CCnGG_A
Cuts at: 2500 3009 3318 2500
Size: 509 309 5285
Fragments arranged by size:
5285 509 309
PmeI GTTT'AAAC
Cuts at: 3407 3407
Size: 6103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4172 4204 5685 5717 4172
Size: 32 1481 32 4558
Fragments arranged by size:
4558 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6103
PsiI TTA'TAA
Cuts at: 3673 3673
Size: 6103
PstI C_TGCA'G
Cuts at: 830 3455 830
Size: 2625 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6103
SalI G'TCGA_C
Cuts at: 1936 3445 1936
Size: 1509 4594
SapI GCTCTTCn'nnn_
Cuts at: 2841 2841
Size: 6103
SbfI CC_TGCA'GG
Cuts at: 3455 3455
Size: 6103
ScaI AGT'ACT
Cuts at: 2575 4351 6063 2575
Size: 1776 1712 2615
Fragments arranged by size:
2615 1776 1712
SfcI C'TryA_G
Cuts at: 826 1045 3261 3451 4586 5267 5458 826
Size: 219 2216 190 1135 681 191 1471
Fragments arranged by size:
2216 1471 1135 681 219 191 190
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6103
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6103
SmaI CCC'GGG
Cuts at: 1872 3430 1872
Size: 1558 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2298 4207 5108 5370 820
Size: 197 926 355 1909 901 262 1553
Fragments arranged by size:
1909 1553 926 901 355 262 197
SnaBI TAC'GTA
Cuts at: 493 3894 493
Size: 3401 2702
SpeI A'CTAG_T
Cuts at: 152 2896 152
Size: 2744 3359
SphI G_CATG'C
Cuts at: 1132 3461 1132
Size: 2329 3774
SspI AAT'ATT
Cuts at: 5 52 2084 3178 4027 5
Size: 47 2032 1094 849 2081
Fragments arranged by size:
2081 2032 1094 849 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6103
StyI C'CwwG_G
Cuts at: 513 1065 2411 3542 513
Size: 552 1346 1131 3074
Fragments arranged by size:
3074 1346 1131 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4130 4147 4300 4485 4930 325
Size: 1255 2550 17 153 185 445 1498
Fragments arranged by size:
2550 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2573 3148 4349
Size: 274 80 33 51 1443 596 575 1201
Cuts at: 4349 6061 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1201 596 575 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2496 2528 6063 6095 2496
Size: 32 3535 32 2504
Fragments arranged by size:
3535 2504 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6103
XbaI T'CTAG_A
Cuts at: 3439 3439
Size: 6103
XhoI C'TCGA_G
Cuts at: 1943 2298 1943
Size: 355 5748
XmaI C'CCGG_G
Cuts at: 1870 3428 1870
Size: 1558 4545
XmnI GAAnn'nnTTC
Cuts at: 3348 4232 3348
Size: 884 5219
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3911 276
Size: 53 83 186 936 2377 2468
Fragments arranged by size:
2468 2377 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
DraIII EcoICRI EcoO109I FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI