Restriction map for
pFN21AB5597
(Circular) (Six-base) MAPSORT of: pFN21AB5597.seq Check: 1644 from: 1 to: 6091
pFN21AB5597 6091 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2974 2974
Size: 6091
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3901 278
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3229 3434 1937
Size: 1292 205 4594
Fragments arranged by size:
4594 1292 205
Acc65I G'GTAC_C
Cuts at: 3069 3412 3069
Size: 343 5748
AclI AA'CG_TT
Cuts at: 1563 4218 4591 1563
Size: 2655 373 3063
Fragments arranged by size:
3063 2655 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2366 3212 4154 5538 5806 1632
Size: 734 846 942 1384 268 1917
Fragments arranged by size:
1917 1384 942 846 734 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5894
AflIII A'CryG_T
Cuts at: 1203 4990 5967 1203
Size: 3787 977 1327
Fragments arranged by size:
3787 1327 977
AgeI A'CCGG_T
Cuts at: 4922 4922
Size: 6091
AhdI GACnn_n'nnGTC
Cuts at: 4820 4820
Size: 6091
AleI CACnn'nnGTG
Cuts at: 1166 2315 1166
Size: 1149 4942
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2333 1184
Size: 1149 4942
AlwNI CAG_nnn'CTG
Cuts at: 2026 2057 2342 2653 2704 2818 2971 3040 3834
Size: 31 285 311 51 114 153 69 794
Cuts at: 3834 5406 2026
Size: 1572 2711
Fragments arranged by size:
2711 1572 794 311 285 153 114 69
51 31
ApaLI G'TGCA_C
Cuts at: 4148 5304 4148
Size: 1156 4935
ApoI r'AATT_y
Cuts at: 1493 2486 3400 3626 3778 5887 1493
Size: 993 914 226 152 2109 1697
Fragments arranged by size:
2109 1697 993 914 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4645 160
Size: 872 3613 1606
Fragments arranged by size:
3613 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
AvaI C'yCGr_G
Cuts at: 1870 1943 2217 3416 1870
Size: 73 274 1199 4545
Fragments arranged by size:
4545 1199 274 73
BamHI G'GATC_C
Cuts at: 3421 3788 3421
Size: 367 5724
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2123 2934 3069 3279 3412
Size: 325 217 627 336 811 135 210 133
Cuts at: 3412 4867 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 811 627 336 325 217 210
135 133
BanII G_rGCy'C
Cuts at: 1391 3017 3410 1391
Size: 1626 393 4072
Fragments arranged by size:
4072 1626 393
BbeI G_GCGC'C
Cuts at: 2938 3283 2938
Size: 345 5746
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3319 928
Size: 971 1420 3700
Fragments arranged by size:
3700 1420 971
BbvCI CC'TCA_GC
Cuts at: 2640 3284 3366 2640
Size: 644 82 5365
Fragments arranged by size:
5365 644 82
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2737 2771 4282 4316 1903
Size: 34 800 34 1511 34 3678
Fragments arranged by size:
3678 1511 800 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2305 3983 5193 2305
Size: 1678 1210 3203
Fragments arranged by size:
3203 1678 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6091
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2573 2759 4702
Size: 107 122 71 631 605 901 186 1943
Cuts at: 4702 136
Size: 1525
Fragments arranged by size:
1943 1525 901 631 605 186 122 107
71
BglII A'GATC_T
Cuts at: 6086 6086
Size: 6091
BlpI GC'TnA_GC
Cuts at: 2654 3508 2654
Size: 854 5237
Bme1580I G_kGCm'C
Cuts at: 1790 2126 4152 5308 1790
Size: 336 2026 1156 2573
Fragments arranged by size:
2573 2026 1156 336
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6091
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3822 4780 442
Size: 416 53 83 399 24 2405 958 1753
Fragments arranged by size:
2405 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2134 2260 2816 3164 4751 1239
Size: 895 126 556 348 1587 2579
Fragments arranged by size:
2579 1587 895 556 348 126
Bpu10I CC'TnA_GC
Cuts at: 2640 3284 3366 2640
Size: 644 82 5365
Fragments arranged by size:
5365 644 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2867 4216 5081 5379 2867
Size: 1349 865 298 3579
Fragments arranged by size:
3579 1349 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2684 4754 882
Size: 617 1185 2070 2219
Fragments arranged by size:
2219 2070 1185 617
BsaAI yAC'GTr
Cuts at: 493 1204 3882 493
Size: 711 2678 2702
Fragments arranged by size:
2702 2678 711
BsaBI GATnn'nnATC
Cuts at: 6085 6085
Size: 6091
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2935 3280 3898 4280
Size: 53 83 186 936 1402 345 618 382
Cuts at: 4280 4915 275
Size: 635 1451
Fragments arranged by size:
1451 1402 936 635 618 382 345 186
83 53
BsaWI w'CCGG_w
Cuts at: 3019 4524 4922 5196 5343 5754 3019
Size: 1505 398 274 147 411 3356
Fragments arranged by size:
3356 1505 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2450 2480 2513 2543 1178
Size: 30 165 30 1047 30 33 30 4726
Fragments arranged by size:
4726 1047 165 33 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 2098 2137 2263 2657 1186
Size: 907 5 39 126 394 4620
Fragments arranged by size:
4620 907 394 126 39 5
BseYI C'CCAG_C
Cuts at: 2306 2324 2329 2335 5294 5790 2306
Size: 18 5 6 2959 496 2607
Fragments arranged by size:
2959 2607 496 18 6 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2573 2693 2831 1163
Size: 573 837 120 138 4423
Fragments arranged by size:
4423 837 573 138 120
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3257 3541 4302 5330 1540
Size: 275 186 1256 284 761 1028 2301
Fragments arranged by size:
2301 1256 1028 761 284 275 186
BsiHKAI G_wGCw'C
Cuts at: 3017 3264 4152 4237 5308 3017
Size: 247 888 85 1071 3800
Fragments arranged by size:
3800 1071 888 247 85
BsmI GAATG_Cn'
Cuts at: 3602 3695 3602
Size: 93 5998
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2126 3017 3264 3410 4152 4237 5308
Size: 399 336 891 247 146 742 85 1071
Cuts at: 5308 1391
Size: 2174
Fragments arranged by size:
2174 1071 891 742 399 336 247 146
85
BspEI T'CCGG_A
Cuts at: 3019 5754 3019
Size: 2735 3356
BspHI T'CATG_A
Cuts at: 2775 3980 5710 2775
Size: 1205 1730 3156
Fragments arranged by size:
3156 1730 1205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2712 2799 2974 3446 844
Size: 1868 87 175 472 3489
Fragments arranged by size:
3489 1868 472 175 87
BsrBI CCG'CTC
Cuts at: 1394 1823 2067 3978 1394
Size: 429 244 1911 3507
Fragments arranged by size:
3507 1911 429 244
BsrDI GCAATG_nn'
Cuts at: 65 1248 3213 3348 4586 4760 65
Size: 1183 1965 135 1238 174 1396
Fragments arranged by size:
1965 1396 1238 1183 174 135
BsrFI r'CCGG_y
Cuts at: 1700 1810 2931 3256 4735 4922 1700
Size: 110 1121 325 1479 187 2869
Fragments arranged by size:
2869 1479 1121 325 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6091
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6091
BssSI C'ACGA_G
Cuts at: 4151 5163 4151
Size: 1012 5079
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2818 794
Size: 2024 4067
BstBI TT'CG_AA
Cuts at: 4895 4895
Size: 6091
BstEII G'GTnAC_C
Cuts at: 1184 2386 1184
Size: 1202 4889
BstXI CCAn_nnnn'nTGG
Cuts at: 2239 2398 2239
Size: 159 5932
BstYI r'GATC_y
Cuts at: 1972 2395 3421 3788 4173 4190 5642 5728 5740
Size: 423 1026 367 385 17 1452 86 12
Cuts at: 5740 6086 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1026 423 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4600
Fragments arranged by size:
4600 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5352
Fragments arranged by size:
5352 683 56
BtsI GCAGTG_nn'
Cuts at: 2055 3051 3607 4401 4421 2055
Size: 996 556 794 20 3725
Fragments arranged by size:
3725 996 794 556 20
ClaI AT'CG_AT
Cuts at: 1554 3246 1554
Size: 1692 4399
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6056
DraI TTT'AAA
Cuts at: 3395 3395
Size: 6091
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5098 809
Size: 507 3782 1802
Fragments arranged by size:
3782 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3254 3538 4427
Size: 54 1425 50 275 18 1424 284 889
Cuts at: 4427 6056 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1424 889 284 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3254 3538 1537
Size: 275 1442 284 4090
Fragments arranged by size:
4090 1442 284 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2360 4021 1353
Size: 1007 1661 3423
Fragments arranged by size:
3423 1661 1007
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4678 5052 5198 387
Size: 1064 138 3089 374 146 1280
Fragments arranged by size:
3089 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3015 3015
Size: 6091
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2134 2260 2366 2816 3164 3212 4154
Size: 393 502 126 106 450 348 48 942
Cuts at: 4154 4751 5538 5806 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 942 787 597 502 450 393 348
268 126 106 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2510 2795 2510
Size: 285 5806
EcoO109I rG'GnC_Cy
Cuts at: 2623 2701 2752 2860 2902 3343 2623
Size: 78 51 108 42 441 5371
Fragments arranged by size:
5371 441 108 78 51 42
EcoRI G'AATT_C
Cuts at: 3400 3778 3400
Size: 378 5713
EcoRV GAT'ATC
Cuts at: 3165 3165
Size: 6091
FspI TGC'GCA
Cuts at: 4597 4597
Size: 6091
HaeII r_GCGC'y
Cuts at: 1678 2254 2938 3283 5238 1678
Size: 576 684 345 1955 2531
Fragments arranged by size:
2531 1955 684 576 345
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2066 2098 2380 2412 2450
Size: 32 135 32 494 32 282 32 38
Cuts at: 2450 2482 2886 2918 3222 3254 4732 4764 4806
Size: 32 404 32 304 32 1478 32 42
Cuts at: 4806 4838 1373
Size: 32 2626
Fragments arranged by size:
2626 1478 494 404 304 282 135 42
38 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3230 3435 3681 669
Size: 988 281 1292 205 246 3079
Fragments arranged by size:
3079 1292 988 281 246 205
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6091
HpaI GTT'AAC
Cuts at: 3681 3681
Size: 6091
KasI G'GCGC_C
Cuts at: 2934 3279 2934
Size: 345 5746
KpnI G_GTAC'C
Cuts at: 3073 3416 3073
Size: 343 5748
MfeI C'AATT_G
Cuts at: 3690 3690
Size: 6091
MluI A'CGCG_T
Cuts at: 5967 5967
Size: 6091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5205 5389 1305
Size: 3900 184 2007
Fragments arranged by size:
3900 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4269
Fragments arranged by size:
4269 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2315 2721 3008 4049 4408 4567 518
Size: 648 1149 406 287 1041 359 159 2042
Fragments arranged by size:
2042 1149 1041 648 406 359 287 159
MspA1I CmG'CkG
Cuts at: 1606 2406 2493 2587 2650 2818 2959 3507 4184
Size: 800 87 94 63 168 141 548 677
Cuts at: 4184 5332 5577 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 800 677 548 245 168 141
94 87 63
NarI GG'CG_CC
Cuts at: 2935 3280 2935
Size: 345 5746
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4600
Fragments arranged by size:
4600 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6091
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6091
NotI GC'GGCC_GC
Cuts at: 3538 3538
Size: 6091
NspI r_CATG'y
Cuts at: 1132 1229 2893 3449 4994 1132
Size: 97 1664 556 1545 2229
Fragments arranged by size:
2229 1664 1545 556 97
PasI CC'CwG_GG
Cuts at: 1348 3097 1348
Size: 1749 4342
PciI A'CATG_T
Cuts at: 4990 4990
Size: 6091
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2119 2342 3040 1108
Size: 1011 223 698 4159
Fragments arranged by size:
4159 1011 698 223
PfoI T'CCnGG_A
Cuts at: 2517 2697 2904 3167 2517
Size: 180 207 263 5441
Fragments arranged by size:
5441 263 207 180
PmeI GTTT'AAAC
Cuts at: 3395 3395
Size: 6091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 5673 5705 4160
Size: 32 1481 32 4546
Fragments arranged by size:
4546 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2701 2860 2902 3343 2701
Size: 159 42 441 5449
Fragments arranged by size:
5449 441 159 42
PshAI GACnn'nnGTC
Cuts at: 1613 2468 1613
Size: 855 5236
PsiI TTA'TAA
Cuts at: 3661 3661
Size: 6091
PstI C_TGCA'G
Cuts at: 830 2419 2760 3443 830
Size: 1589 341 683 3478
Fragments arranged by size:
3478 1589 683 341
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6091
PvuII CAG'CTG
Cuts at: 2493 2587 2650 2959 2493
Size: 94 63 309 5625
Fragments arranged by size:
5625 309 94 63
SacI G_AGCT'C
Cuts at: 3017 3017
Size: 6091
SalI G'TCGA_C
Cuts at: 1936 3228 3433 1936
Size: 1292 205 4594
Fragments arranged by size:
4594 1292 205
SbfI CC_TGCA'GG
Cuts at: 2760 3443 2760
Size: 683 5408
ScaI AGT'ACT
Cuts at: 4339 6051 4339
Size: 1712 4379
SfcI C'TryA_G
Cuts at: 826 1045 2415 2756 3439 4574 5255 5446 826
Size: 219 1370 341 683 1135 681 191 1471
Fragments arranged by size:
1471 1370 1135 683 681 341 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2573 2759 2573
Size: 186 5905
SfoI GGC'GCC
Cuts at: 2936 3281 2936
Size: 345 5746
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6091
SmaI CCC'GGG
Cuts at: 1872 2219 3418 1872
Size: 347 1199 4545
Fragments arranged by size:
4545 1199 347
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2882 4195 5096 5358 820
Size: 197 926 939 1313 901 262 1553
Fragments arranged by size:
1553 1313 939 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3882 493
Size: 3389 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6091
SphI G_CATG'C
Cuts at: 1132 3449 1132
Size: 2317 3774
SspI AAT'ATT
Cuts at: 5 52 4015 5
Size: 47 3963 2081
Fragments arranged by size:
3963 2081 47
StuI AGG'CCT
Cuts at: 1517 2117 1517
Size: 600 5491
StyI C'CwwG_G
Cuts at: 513 1065 2004 2118 2391 2685 3111 3530 513
Size: 552 939 114 273 294 426 419 3074
Fragments arranged by size:
3074 939 552 426 419 294 273 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2338 2607 4118 4135 4288 4473 4918
Size: 1255 758 269 1511 17 153 185 445
Cuts at: 4918 325
Size: 1498
Fragments arranged by size:
1511 1498 1255 758 445 269 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4337 6049 96
Size: 274 80 33 51 1443 2360 1712 138
Fragments arranged by size:
2360 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3163 3195 3243 3275 6051 6083 3163
Size: 32 48 32 2776 32 3171
Fragments arranged by size:
3171 2776 48 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6091
XbaI T'CTAG_A
Cuts at: 3427 3427
Size: 6091
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6091
XmaI C'CCGG_G
Cuts at: 1870 2217 3416 1870
Size: 347 1199 4545
Fragments arranged by size:
4545 1199 347
XmnI GAAnn'nnTTC
Cuts at: 2171 4220 2171
Size: 2049 4042
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3899 276
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SrfI SwaI
XcmI