Restriction map for
pFN21AB5596
(Circular) (Six-base) MAPSORT of: pFN21AB5596.seq Check: 23 from: 1 to: 6688
pFN21AB5596 6688 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3180 3180
Size: 6688
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4498 278
Size: 53 83 186 936 2962 2468
Fragments arranged by size:
2962 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4031 1937
Size: 2094 4594
Acc65I G'GTAC_C
Cuts at: 4009 4009
Size: 6688
AclI AA'CG_TT
Cuts at: 1563 2612 4815 5188 1563
Size: 1049 2203 373 3063
Fragments arranged by size:
3063 2203 1049 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2846 4751 6135 6403 1632
Size: 1214 1905 1384 268 1917
Fragments arranged by size:
1917 1905 1384 1214 268
AfeI AGC'GCT
Cuts at: 3383 3383
Size: 6688
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6491
AflIII A'CryG_T
Cuts at: 1203 3842 5587 6564 1203
Size: 2639 1745 977 1327
Fragments arranged by size:
2639 1745 1327 977
AgeI A'CCGG_T
Cuts at: 5519 5519
Size: 6688
AhdI GACnn_n'nnGTC
Cuts at: 3396 5417 3396
Size: 2021 4667
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6688
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6688
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3486 3518 3486
Size: 32 6656
AlwNI CAG_nnn'CTG
Cuts at: 4431 6003 4431
Size: 1572 5116
ApaLI G'TGCA_C
Cuts at: 4745 5901 4745
Size: 1156 5532
ApoI r'AATT_y
Cuts at: 1493 2489 3089 3528 3997 4223 4375 6484 1493
Size: 996 600 439 469 226 152 2109 1697
Fragments arranged by size:
2109 1697 996 600 469 439 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5242 160
Size: 872 4210 1606
Fragments arranged by size:
4210 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6688
AvaI C'yCGr_G
Cuts at: 1870 1943 4013 1870
Size: 73 2070 4545
Fragments arranged by size:
4545 2070 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3123 3156 3123
Size: 33 6655
BamHI G'GATC_C
Cuts at: 4018 4385 4018
Size: 367 6321
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2949 4009 5464 618
Size: 325 217 627 1162 1060 1455 1842
Fragments arranged by size:
1842 1455 1162 1060 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2181 2424 4007 1391
Size: 790 243 1583 4072
Fragments arranged by size:
4072 1583 790 243
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2799 3348 3759 928
Size: 971 900 549 411 3857
Fragments arranged by size:
3857 971 900 549 411
BbvCI CC'TCA_GC
Cuts at: 2269 2269
Size: 6688
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4879 4913 1903
Size: 34 50 34 2858 34 3678
Fragments arranged by size:
3678 2858 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3157 4580 5790 3157
Size: 1423 1210 4055
Fragments arranged by size:
4055 1423 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6688
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2018 5299
Size: 107 122 71 631 605 337 9 3281
Cuts at: 5299 136
Size: 1525
Fragments arranged by size:
3281 1525 631 605 337 122 107 71
9
BglII A'GATC_T
Cuts at: 6683 6683
Size: 6688
BlpI GC'TnA_GC
Cuts at: 4105 4105
Size: 6688
Bme1580I G_kGCm'C
Cuts at: 1790 3840 4749 5905 1790
Size: 2050 909 1156 2573
Fragments arranged by size:
2573 2050 1156 909
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6688
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2342 2362 2527
Size: 416 53 83 399 24 925 20 165
Cuts at: 2527 4419 5377 442
Size: 1892 958 1753
Fragments arranged by size:
1892 1753 958 925 416 399 165 83
53 24 20
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6688
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3351 3351
Size: 6688
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2696 2714 3414 5348 1239
Size: 1457 18 700 1934 2579
Fragments arranged by size:
2579 1934 1457 700 18
Bpu10I CC'TnA_GC
Cuts at: 2269 2416 2269
Size: 147 6541
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2330 3505 4813 5678 5976 2330
Size: 1175 1308 865 298 3042
Fragments arranged by size:
3042 1308 1175 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5351 882
Size: 617 3852 2219
Fragments arranged by size:
3852 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2801 3845 4479 493
Size: 711 1597 1044 634 2702
Fragments arranged by size:
2702 1597 1044 711 634
BsaBI GATnn'nnATC
Cuts at: 2979 3435 6682 2979
Size: 456 3247 2985
Fragments arranged by size:
3247 2985 456
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2726 4495 4877 5512
Size: 53 83 186 936 1193 1769 382 635
Cuts at: 5512 275
Size: 1451
Fragments arranged by size:
1769 1451 1193 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3160 5121 5519 5793 5940 6351 3160
Size: 1961 398 274 147 411 3497
Fragments arranged by size:
3497 1961 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2898 2928 1178
Size: 30 165 30 1495 30 4938
Fragments arranged by size:
4938 1495 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2040 2043 2046 2049 2073 2076 2299 2432
Size: 854 3 3 3 24 3 223 133
Cuts at: 2432 2559 1186
Size: 127 5315
Fragments arranged by size:
5315 854 223 133 127 24 3 3
3 3
BseYI C'CCAG_C
Cuts at: 5891 6387 5891
Size: 496 6192
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6115
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2259 4138 4899 5927 1540
Size: 275 186 258 1879 761 1028 2301
Fragments arranged by size:
2301 1879 1028 761 275 258 186
BsiHKAI G_wGCw'C
Cuts at: 4749 4834 5905 4749
Size: 85 1071 5532
Fragments arranged by size:
5532 1071 85
BsmI GAATG_Cn'
Cuts at: 2563 2751 3974 4199 4292 2563
Size: 188 1223 225 93 4959
Fragments arranged by size:
4959 1223 225 188 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 2424 3840 4007 4749 4834 5905
Size: 399 391 243 1416 167 742 85 1071
Cuts at: 5905 1391
Size: 2174
Fragments arranged by size:
2174 1416 1071 742 399 391 243 167
85
BspEI T'CCGG_A
Cuts at: 3160 6351 3160
Size: 3191 3497
BspHI T'CATG_A
Cuts at: 3337 4577 6307 3337
Size: 1240 1730 3718
Fragments arranged by size:
3718 1730 1240
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3180 4043 844
Size: 2336 863 3489
Fragments arranged by size:
3489 2336 863
BsrBI CCG'CTC
Cuts at: 1394 1823 4575 1394
Size: 429 2752 3507
Fragments arranged by size:
3507 2752 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3476 5183 5357 65
Size: 1183 2228 1707 174 1396
Fragments arranged by size:
2228 1707 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5332 5519 1700
Size: 110 3522 187 2869
Fragments arranged by size:
3522 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3298 96
Size: 3202 3486
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6688
BssSI C'ACGA_G
Cuts at: 4748 5760 4748
Size: 1012 5676
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6688
BstBI TT'CG_AA
Cuts at: 5492 5492
Size: 6688
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6688
BstXI CCAn_nnnn'nTGG
Cuts at: 2174 2174
Size: 6688
BstYI r'GATC_y
Cuts at: 1972 4018 4385 4770 4787 6239 6325 6337 6683
Size: 2046 367 385 17 1452 86 12 346
Cuts at: 6683 1972
Size: 1977
Fragments arranged by size:
2046 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2151 2897 513
Size: 552 1086 746 4304
Fragments arranged by size:
4304 1086 746 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3745 487
Size: 683 56 2519 3430
Fragments arranged by size:
3430 2519 683 56
BtsI GCAGTG_nn'
Cuts at: 3778 4204 4998 5018 3778
Size: 426 794 20 5448
Fragments arranged by size:
5448 794 426 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6688
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6653
DraI TTT'AAA
Cuts at: 3992 3992
Size: 6688
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5695 809
Size: 507 4379 1802
Fragments arranged by size:
4379 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2256 2319 3616
Size: 54 1425 50 275 18 426 63 1297
Cuts at: 3616 3891 4135 5024 6653 8
Size: 275 244 889 1629 43
Fragments arranged by size:
1629 1425 1297 889 426 275 275 244
63 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2256 4135 1537
Size: 275 444 1879 4090
Fragments arranged by size:
4090 1879 444 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2317 3957 4618 1353
Size: 964 1640 661 3423
Fragments arranged by size:
3423 1640 964 661
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2179 2188 2281 5275 5649 5795
Size: 1064 138 590 9 93 2994 374 146
Cuts at: 5795 387
Size: 1280
Fragments arranged by size:
2994 1280 1064 590 374 146 138 93
9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2696 2714 2846 3414 4751 5348 6135
Size: 393 1064 18 132 568 1337 597 787
Cuts at: 6135 6403 1239
Size: 268 1524
Fragments arranged by size:
1524 1337 1064 787 597 568 393 268
132 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2921 2921
Size: 6688
EcoO109I rG'GnC_Cy
Cuts at: 2158 2689 3550 2158
Size: 531 861 5296
Fragments arranged by size:
5296 861 531
EcoRI G'AATT_C
Cuts at: 3528 3997 4375 3528
Size: 469 378 5841
Fragments arranged by size:
5841 469 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3378 3378
Size: 6688
FspI TGC'GCA
Cuts at: 5194 5194
Size: 6688
HaeII r_GCGC'y
Cuts at: 1678 3385 3900 5835 1678
Size: 1707 515 1935 2531
Fragments arranged by size:
2531 1935 1707 515
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2422 2454 2978 3010 3260
Size: 32 135 32 850 32 524 32 250
Cuts at: 3260 3292 3382 3414 5329 5361 5403 5435 1373
Size: 32 90 32 1915 32 42 32 2626
Fragments arranged by size:
2626 1915 850 524 250 135 90 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4032 4278 669
Size: 988 281 2094 246 3079
Fragments arranged by size:
3079 2094 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6688
HpaI GTT'AAC
Cuts at: 4278 4278
Size: 6688
KpnI G_GTAC'C
Cuts at: 4013 4013
Size: 6688
MfeI C'AATT_G
Cuts at: 3819 4287 3819
Size: 468 6220
MluI A'CGCG_T
Cuts at: 6564 6564
Size: 6688
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5802 5986 1305
Size: 4497 184 2007
Fragments arranged by size:
4497 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2321 3893 10
Size: 54 1425 343 489 1572 2805
Fragments arranged by size:
2805 1572 1425 489 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2172 2409 3069 3207 4646 5005 5164
Size: 648 1006 237 660 138 1439 359 159
Cuts at: 5164 518
Size: 2042
Fragments arranged by size:
2042 1439 1006 660 648 359 237 159
138
MspA1I CmG'CkG
Cuts at: 1606 2153 2285 2731 4104 4781 5929 6174 1606
Size: 547 132 446 1373 677 1148 245 2120
Fragments arranged by size:
2120 1373 1148 677 547 446 245 132
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6136
NdeI CA'TA_TG
Cuts at: 387 3146 387
Size: 2759 3929
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6688
NotI GC'GGCC_GC
Cuts at: 2256 4135 2256
Size: 1879 4809
NspI r_CATG'y
Cuts at: 1132 1229 3206 4046 5591 1132
Size: 97 1977 840 1545 2229
Fragments arranged by size:
2229 1977 1545 840 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6688
PciI A'CATG_T
Cuts at: 5587 5587
Size: 6688
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6688
PfoI T'CCnGG_A
Cuts at: 2691 2691
Size: 6688
PmeI GTTT'AAAC
Cuts at: 3992 3992
Size: 6688
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4757 4789 6270 6302 4757
Size: 32 1481 32 5143
Fragments arranged by size:
5143 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2158 2689 3550 2158
Size: 531 861 5296
Fragments arranged by size:
5296 861 531
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6688
PsiI TTA'TAA
Cuts at: 4258 4258
Size: 6688
PstI C_TGCA'G
Cuts at: 830 2655 4040 830
Size: 1825 1385 3478
Fragments arranged by size:
3478 1825 1385
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6688
PvuII CAG'CTG
Cuts at: 2285 2285
Size: 6688
SacII CC_GC'GG
Cuts at: 2154 2154
Size: 6688
SalI G'TCGA_C
Cuts at: 1936 4030 1936
Size: 2094 4594
SbfI CC_TGCA'GG
Cuts at: 4040 4040
Size: 6688
ScaI AGT'ACT
Cuts at: 4936 6648 4936
Size: 1712 4976
SfcI C'TryA_G
Cuts at: 826 1045 2651 4036 5171 5852 6043 826
Size: 219 1606 1385 1135 681 191 1471
Fragments arranged by size:
1606 1471 1385 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6688
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6688
SmaI CCC'GGG
Cuts at: 1872 4015 1872
Size: 2143 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2345 3484 4792 5693 5955 820
Size: 197 926 402 1139 1308 901 262 1553
Fragments arranged by size:
1553 1308 1139 926 901 402 262 197
SnaBI TAC'GTA
Cuts at: 493 4479 493
Size: 3986 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6688
SphI G_CATG'C
Cuts at: 1132 4046 1132
Size: 2914 3774
SspI AAT'ATT
Cuts at: 5 52 3958 4612 5
Size: 47 3906 654 2081
Fragments arranged by size:
3906 2081 654 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6688
StyI C'CwwG_G
Cuts at: 513 1065 2316 4127 513
Size: 552 1251 1811 3074
Fragments arranged by size:
3074 1811 1251 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4715 4732 4885 5070 5515 325
Size: 1255 3135 17 153 185 445 1498
Fragments arranged by size:
3135 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3298 3571 4934
Size: 274 80 33 51 1443 1321 273 1363
Cuts at: 4934 6646 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1363 1321 274 273 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2613 2645 6648 6680 2613
Size: 32 4003 32 2621
Fragments arranged by size:
4003 2621 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6688
XbaI T'CTAG_A
Cuts at: 2983 4024 2983
Size: 1041 5647
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2329 2329
Size: 6688
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6688
XmaI C'CCGG_G
Cuts at: 1870 4013 1870
Size: 2143 4545
XmnI GAAnn'nnTTC
Cuts at: 4817 4817
Size: 6688
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4496 276
Size: 53 83 186 936 2962 2468
Fragments arranged by size:
2962 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI
SrfI SwaI