Restriction map for
pFN21AB5592
(Circular) (Six-base) MAPSORT of: pFN21AB5592.seq Check: 4875 from: 1 to: 6187
pFN21AB5592 6187 bp
With 182 enzymes: SgfI *
June 18, 2013 12:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2475 3053 2475
Size: 578 5609
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3997 278
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3530 1937
Size: 1593 4594
Acc65I G'GTAC_C
Cuts at: 3508 3508
Size: 6187
AclI AA'CG_TT
Cuts at: 1563 4314 4687 1563
Size: 2751 373 3063
Fragments arranged by size:
3063 2751 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2005 3339 4250 5634 5902 1632
Size: 373 1334 911 1384 268 1917
Fragments arranged by size:
1917 1384 1334 911 373 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5990
AflIII A'CryG_T
Cuts at: 1203 2471 3146 5086 6063 1203
Size: 1268 675 1940 977 1327
Fragments arranged by size:
1940 1327 1268 977 675
AgeI A'CCGG_T
Cuts at: 5018 5018
Size: 6187
AhdI GACnn_n'nnGTC
Cuts at: 4916 4916
Size: 6187
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6187
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6187
AlwNI CAG_nnn'CTG
Cuts at: 3366 3930 5502 3366
Size: 564 1572 4051
Fragments arranged by size:
4051 1572 564
ApaI G_GGCC'C
Cuts at: 2265 2265
Size: 6187
ApaLI G'TGCA_C
Cuts at: 4244 5400 4244
Size: 1156 5031
ApoI r'AATT_y
Cuts at: 1493 2437 2531 2654 3496 3722 3874 5983 1493
Size: 944 94 123 842 226 152 2109 1697
Fragments arranged by size:
2109 1697 944 842 226 152 123 94
AseI AT'TA_AT
Cuts at: 160 1032 4741 160
Size: 872 3709 1606
Fragments arranged by size:
3709 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
AvaI C'yCGr_G
Cuts at: 1870 1943 2133 2176 3274 3512 1870
Size: 73 190 43 1098 238 4545
Fragments arranged by size:
4545 1098 238 190 73 43
BamHI G'GATC_C
Cuts at: 3517 3884 3517
Size: 367 5820
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2114 3508 4963 618
Size: 325 217 627 327 1394 1455 1842
Fragments arranged by size:
1842 1455 1394 627 327 325 217
BanII G_rGCy'C
Cuts at: 1391 2265 3506 1391
Size: 874 1241 4072
Fragments arranged by size:
4072 1241 874
BbeI G_GCGC'C
Cuts at: 2118 2118
Size: 6187
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3117 928
Size: 971 1218 3998
Fragments arranged by size:
3998 1218 971
BbvCI CC'TCA_GC
Cuts at: 2964 2964
Size: 6187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2239 2273 4378 4412 1903
Size: 34 50 34 218 34 2105 34 3678
Fragments arranged by size:
3678 2105 218 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4079 5289 4079
Size: 1210 4977
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6187
BfrBI ATG'CAT
Cuts at: 2559 3455 2559
Size: 896 5291
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4798 136
Size: 107 122 71 631 605 3126 1525
Fragments arranged by size:
3126 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6182 6182
Size: 6187
BlpI GC'TnA_GC
Cuts at: 3604 3604
Size: 6187
Bme1580I G_kGCm'C
Cuts at: 1790 2265 4248 5404 1790
Size: 475 1983 1156 2573
Fragments arranged by size:
2573 1983 1156 475
BmgBI CAC'GTC
Cuts at: 1318 2700 1318
Size: 1382 4805
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2787 2794 3918
Size: 416 53 83 399 24 1370 7 1124
Cuts at: 3918 4876 442
Size: 958 1753
Fragments arranged by size:
1753 1370 1124 958 416 399 83 53
24 7
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2688 3039 4847 1239
Size: 1449 351 1808 2579
Fragments arranged by size:
2579 1808 1449 351
Bpu10I CC'TnA_GC
Cuts at: 2964 2964
Size: 6187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4312 5177 5475 4312
Size: 865 298 5024
Fragments arranged by size:
5024 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2288 3389 4850 882
Size: 617 789 1101 1461 2219
Fragments arranged by size:
2219 1461 1101 789 617
BsaAI yAC'GTr
Cuts at: 493 1204 2236 3978 493
Size: 711 1032 1742 2702
Fragments arranged by size:
2702 1742 1032 711
BsaBI GATnn'nnATC
Cuts at: 2769 6181 2769
Size: 3412 2775
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2042 2115 2220 3994
Size: 53 83 186 936 509 73 105 1774
Cuts at: 3994 4376 5011 275
Size: 382 635 1451
Fragments arranged by size:
1774 1451 936 635 509 382 186 105
83 73 53
BsaWI w'CCGG_w
Cuts at: 4620 5018 5292 5439 5850 4620
Size: 398 274 147 411 4957
Fragments arranged by size:
4957 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2299 2329 1178
Size: 30 165 30 896 30 5036
Fragments arranged by size:
5036 896 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2116 2981 1186
Size: 930 865 4392
Fragments arranged by size:
4392 930 865
BseYI C'CCAG_C
Cuts at: 2060 3432 5390 5886 2060
Size: 1372 1958 496 2361
Fragments arranged by size:
2361 1958 1372 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2337 2935 1163
Size: 573 601 598 4415
Fragments arranged by size:
4415 601 598 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2200 3637 4398 5426 1540
Size: 275 186 199 1437 761 1028 2301
Fragments arranged by size:
2301 1437 1028 761 275 199 186
BsiHKAI G_wGCw'C
Cuts at: 2312 2940 3275 4248 4333 5404 2312
Size: 628 335 973 85 1071 3095
Fragments arranged by size:
3095 1071 973 628 335 85
BsmI GAATG_Cn'
Cuts at: 2561 3434 3698 3791 2561
Size: 873 264 93 4957
Fragments arranged by size:
4957 873 264 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2706 2706
Size: 6187
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2265 2312 2940 3275 3506 4248 4333
Size: 399 475 47 628 335 231 742 85
Cuts at: 4333 5404 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 628 475 399 335 231
85 47
BspEI T'CCGG_A
Cuts at: 5850 5850
Size: 6187
BspHI T'CATG_A
Cuts at: 4076 5806 4076
Size: 1730 4457
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2456 2475 3053 3542 844
Size: 1612 19 578 489 3489
Fragments arranged by size:
3489 1612 578 489 19
BsrBI CCG'CTC
Cuts at: 1394 1823 4074 1394
Size: 429 2251 3507
Fragments arranged by size:
3507 2251 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4682 4856 65
Size: 1183 3434 174 1396
Fragments arranged by size:
3434 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4831 5018 1700
Size: 110 3021 187 2869
Fragments arranged by size:
3021 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3392 96
Size: 3296 2891
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6187
BssSI C'ACGA_G
Cuts at: 4247 5259 4247
Size: 1012 5175
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2718 3270 794
Size: 1924 552 3711
Fragments arranged by size:
3711 1924 552
BstBI TT'CG_AA
Cuts at: 4991 4991
Size: 6187
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6187
BstYI r'GATC_y
Cuts at: 1972 2998 3517 3884 4269 4286 5738 5824 5836
Size: 1026 519 367 385 17 1452 86 12
Cuts at: 5836 6182 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1026 519 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2194 2323 2593 3139 3206 513
Size: 552 1129 129 270 546 67 3494
Fragments arranged by size:
3494 1129 552 546 270 129 67
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5448
Fragments arranged by size:
5448 683 56
BtsI GCAGTG_nn'
Cuts at: 3703 4497 4517 3703
Size: 794 20 5373
Fragments arranged by size:
5373 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6187
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6152
DraI TTT'AAA
Cuts at: 3491 3491
Size: 6187
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2219 5194 809
Size: 507 903 2975 1802
Fragments arranged by size:
2975 1802 903 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2108 2197 3634
Size: 54 1425 50 275 18 278 89 1437
Cuts at: 3634 4523 6152 8
Size: 889 1629 43
Fragments arranged by size:
1629 1437 1425 889 278 275 89 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2197 3634 1537
Size: 275 385 1437 4090
Fragments arranged by size:
4090 1437 385 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3315 4117 1353
Size: 1962 802 3423
Fragments arranged by size:
3423 1962 802
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4774 5148 5294 387
Size: 1064 138 3185 374 146 1280
Fragments arranged by size:
3185 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2005 2688 3039 3339 4250 4847 5634
Size: 393 373 683 351 300 911 597 787
Cuts at: 5634 5902 1239
Size: 268 1524
Fragments arranged by size:
1524 911 787 683 597 393 373 351
300 268
EcoO109I rG'GnC_Cy
Cuts at: 2262 2262
Size: 6187
EcoRI G'AATT_C
Cuts at: 2437 2654 3496 3874 2437
Size: 217 842 378 4750
Fragments arranged by size:
4750 842 378 217
EcoRV GAT'ATC
Cuts at: 2668 2668
Size: 6187
FspI TGC'GCA
Cuts at: 4693 4693
Size: 6187
HaeII r_GCGC'y
Cuts at: 1678 2118 5334 1678
Size: 440 3216 2531
Fragments arranged by size:
3216 2531 440
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4828 4860 4902 4934 1373
Size: 32 135 32 3256 32 42 32 2626
Fragments arranged by size:
3256 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3531 3777 669
Size: 988 281 1593 246 3079
Fragments arranged by size:
3079 1593 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2083 748
Size: 1335 4852
HpaI GTT'AAC
Cuts at: 3777 3777
Size: 6187
KasI G'GCGC_C
Cuts at: 2114 2114
Size: 6187
KpnI G_GTAC'C
Cuts at: 3512 3512
Size: 6187
MfeI C'AATT_G
Cuts at: 3786 3786
Size: 6187
MluI A'CGCG_T
Cuts at: 6063 6063
Size: 6187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2124 2798 5301 5485 1305
Size: 819 674 2503 184 2007
Fragments arranged by size:
2503 2007 819 674 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4365
Fragments arranged by size:
4365 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4145 4504 4663 518
Size: 648 2979 359 159 2042
Fragments arranged by size:
2979 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2072 2208 2963 3105 3208 3603 4280 5428
Size: 466 136 755 142 103 395 677 1148
Cuts at: 5428 5673 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 755 677 466 395 245 142
136 103
NarI GG'CG_CC
Cuts at: 2115 2115
Size: 6187
NcoI C'CATG_G
Cuts at: 513 1065 2323 2593 513
Size: 552 1258 270 4107
Fragments arranged by size:
4107 1258 552 270
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6187
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6187
NotI GC'GGCC_GC
Cuts at: 3634 3634
Size: 6187
NsiI A_TGCA'T
Cuts at: 2561 3457 2561
Size: 896 5291
NspI r_CATG'y
Cuts at: 1132 1229 2254 2475 3150 3459 3545 5090 1132
Size: 97 1025 221 675 309 86 1545 2229
Fragments arranged by size:
2229 1545 1025 675 309 221 97 86
PasI CC'CwG_GG
Cuts at: 1348 2676 1348
Size: 1328 4859
PciI A'CATG_T
Cuts at: 2471 3146 5086 2471
Size: 675 1940 3572
Fragments arranged by size:
3572 1940 675
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6187
PfoI T'CCnGG_A
Cuts at: 3055 3055
Size: 6187
PmeI GTTT'AAAC
Cuts at: 3491 3491
Size: 6187
PmlI CAC'GTG
Cuts at: 2236 2236
Size: 6187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4256 4288 5769 5801 4256
Size: 32 1481 32 4642
Fragments arranged by size:
4642 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6187
PsiI TTA'TAA
Cuts at: 3757 3757
Size: 6187
PspOMI G'GGCC_C
Cuts at: 2261 2261
Size: 6187
PstI C_TGCA'G
Cuts at: 830 2126 2723 3539 830
Size: 1296 597 816 3478
Fragments arranged by size:
3478 1296 816 597
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6187
PvuII CAG'CTG
Cuts at: 2072 2963 2072
Size: 891 5296
SacII CC_GC'GG
Cuts at: 3209 3209
Size: 6187
SalI G'TCGA_C
Cuts at: 1936 3529 1936
Size: 1593 4594
SbfI CC_TGCA'GG
Cuts at: 2126 3539 2126
Size: 1413 4774
ScaI AGT'ACT
Cuts at: 4435 6147 4435
Size: 1712 4475
SfcI C'TryA_G
Cuts at: 826 1045 2122 2719 2830 3535 4670 5351 5542
Size: 219 1077 597 111 705 1135 681 191
Cuts at: 5542 826
Size: 1471
Fragments arranged by size:
1471 1135 1077 705 681 597 219 191
111
SfoI GGC'GCC
Cuts at: 2116 2116
Size: 6187
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6187
SmaI CCC'GGG
Cuts at: 1872 2135 2178 3514 1872
Size: 263 43 1336 4545
Fragments arranged by size:
4545 1336 263 43
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4291 5192 5454 820
Size: 197 926 2348 901 262 1553
Fragments arranged by size:
2348 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3978 493
Size: 3485 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6187
SphI G_CATG'C
Cuts at: 1132 3545 1132
Size: 2413 3774
SspI AAT'ATT
Cuts at: 5 52 4111 5
Size: 47 4059 2081
Fragments arranged by size:
4059 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6187
StyI C'CwwG_G
Cuts at: 513 1065 2323 2593 3626 513
Size: 552 1258 270 1033 3074
Fragments arranged by size:
3074 1258 1033 552 270
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2275 2862 2955 4214 4231 4384 4569
Size: 1255 695 587 93 1259 17 153 185
Cuts at: 4569 5014 325
Size: 445 1498
Fragments arranged by size:
1498 1259 1255 695 587 445 185 153
93 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3392 4433 6145
Size: 274 80 33 51 1443 1415 1041 1712
Cuts at: 6145 96
Size: 138
Fragments arranged by size:
1712 1443 1415 1041 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2299 2331 2917 2930 2949 2962 3034 3066 6147
Size: 32 586 13 19 13 72 32 3081
Cuts at: 6147 6179 2299
Size: 32 2307
Fragments arranged by size:
3081 2307 586 72 32 32 32 19
13 13
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6187
XbaI T'CTAG_A
Cuts at: 3523 3523
Size: 6187
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2366 2366
Size: 6187
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6187
XmaI C'CCGG_G
Cuts at: 1870 2133 2176 3512 1870
Size: 263 43 1336 4545
Fragments arranged by size:
4545 1336 263 43
XmnI GAAnn'nnTTC
Cuts at: 4316 4316
Size: 6187
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3995 276
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BplI BsiWI BstXI
BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI
NaeI NgoMIV NruI PacI PpuMI PspXI PsrI RsrII
SacI SanDI SapI SexAI SfiI SrfI SwaI