Restriction map for
pFN21AB5572
(Circular) (Six-base) MAPSORT of: pFN21AB5572.seq Check: 2424 from: 1 to: 5848
pFN21AB5572 5848 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2087 2087
Size: 5848
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3658 278
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3191 1937
Size: 1254 4594
Acc65I G'GTAC_C
Cuts at: 3169 3169
Size: 5848
AclI AA'CG_TT
Cuts at: 1563 3975 4348 1563
Size: 2412 373 3063
Fragments arranged by size:
3063 2412 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2285 2645 3911 5295 5563 1632
Size: 653 360 1266 1384 268 1917
Fragments arranged by size:
1917 1384 1266 653 360 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5651
AflIII A'CryG_T
Cuts at: 1203 2369 4747 5724 1203
Size: 1166 2378 977 1327
Fragments arranged by size:
2378 1327 1166 977
AgeI A'CCGG_T
Cuts at: 4679 4679
Size: 5848
AhdI GACnn_n'nnGTC
Cuts at: 4577 4577
Size: 5848
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5848
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5848
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2406 2438 2406
Size: 32 5816
AlwNI CAG_nnn'CTG
Cuts at: 2301 3591 5163 2301
Size: 1290 1572 2986
Fragments arranged by size:
2986 1572 1290
ApaLI G'TGCA_C
Cuts at: 2365 3905 5061 2365
Size: 1540 1156 3152
Fragments arranged by size:
3152 1540 1156
ApoI r'AATT_y
Cuts at: 1493 3137 3157 3383 3535 5644 1493
Size: 1644 20 226 152 2109 1697
Fragments arranged by size:
2109 1697 1644 226 152 20
AseI AT'TA_AT
Cuts at: 160 1032 4402 160
Size: 872 3370 1606
Fragments arranged by size:
3370 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
AvaI C'yCGr_G
Cuts at: 1870 1943 3173 1870
Size: 73 1230 4545
Fragments arranged by size:
4545 1230 73
BamHI G'GATC_C
Cuts at: 3178 3545 3178
Size: 367 5481
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2110 2246 2381 3169 4624
Size: 325 217 627 323 136 135 788 1455
Cuts at: 4624 618
Size: 1842
Fragments arranged by size:
1842 1455 788 627 325 323 217 136
135
BanII G_rGCy'C
Cuts at: 1391 2012 2284 3167 1391
Size: 621 272 883 4072
Fragments arranged by size:
4072 883 621 272
BbeI G_GCGC'C
Cuts at: 2114 2250 2385 2114
Size: 136 135 5577
Fragments arranged by size:
5577 136 135
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4877
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4039 4073 1903
Size: 34 2102 34 3678
Fragments arranged by size:
3678 2102 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3740 4950 3740
Size: 1210 4638
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5848
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2541 4459 136
Size: 107 122 71 631 605 869 1918 1525
Fragments arranged by size:
1918 1525 869 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5843 5843
Size: 5848
BlpI GC'TnA_GC
Cuts at: 3265 3265
Size: 5848
Bme1580I G_kGCm'C
Cuts at: 1790 2369 3909 5065 1790
Size: 579 1540 1156 2573
Fragments arranged by size:
2573 1540 1156 579
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5848
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2710 3579 4537
Size: 416 53 83 399 24 1293 869 958
Cuts at: 4537 442
Size: 1753
Fragments arranged by size:
1753 1293 958 869 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2126 2299 4508 1239
Size: 887 173 2209 2579
Fragments arranged by size:
2579 2209 887 173
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3973 4838 5136 3973
Size: 865 298 4685
Fragments arranged by size:
4685 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4511 882
Size: 617 3012 2219
Fragments arranged by size:
3012 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3639 493
Size: 711 2435 2702
Fragments arranged by size:
2702 2435 711
BsaBI GATnn'nnATC
Cuts at: 5842 5842
Size: 5848
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2111 2247 2382 3655
Size: 53 83 186 936 578 136 135 1273
Cuts at: 3655 4037 4672 275
Size: 382 635 1451
Fragments arranged by size:
1451 1273 936 635 578 382 186 136
135 83 53
BsaWI w'CCGG_w
Cuts at: 4281 4679 4953 5100 5511 4281
Size: 398 274 147 411 4618
Fragments arranged by size:
4618 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2406 2436 1178
Size: 30 165 30 1003 30 4590
Fragments arranged by size:
4590 1003 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2495 1186
Size: 1309 4539
BseYI C'CCAG_C
Cuts at: 2551 2979 5051 5547 2551
Size: 428 2072 496 2852
Fragments arranged by size:
2852 2072 496 428
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2956 1163
Size: 573 1220 4055
Fragments arranged by size:
4055 1220 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2965 3298 4059 5087 1540
Size: 275 186 964 333 761 1028 2301
Fragments arranged by size:
2301 1028 964 761 333 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 2284 2369 2723 3909 3994 5065 2012
Size: 272 85 354 1186 85 1071 2795
Fragments arranged by size:
2795 1186 1071 354 272 85 85
BsmI GAATG_Cn'
Cuts at: 2240 2394 3359 3452 2240
Size: 154 965 93 4636
Fragments arranged by size:
4636 965 154 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2284 2369 2723 3167 3909 3994
Size: 399 222 272 85 354 444 742 85
Cuts at: 3994 5065 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 444 399 354 272 222
85 85
BspEI T'CCGG_A
Cuts at: 5511 5511
Size: 5848
BspHI T'CATG_A
Cuts at: 3045 3737 5467 3045
Size: 692 1730 3426
Fragments arranged by size:
3426 1730 692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2087 3203 844
Size: 1243 1116 3489
Fragments arranged by size:
3489 1243 1116
BsrBI CCG'CTC
Cuts at: 1394 1823 2261 3735 1394
Size: 429 438 1474 3507
Fragments arranged by size:
3507 1474 438 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4343 4517 65
Size: 1183 3095 174 1396
Fragments arranged by size:
3095 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2931 4492 4679 1700
Size: 110 1121 1561 187 2869
Fragments arranged by size:
2869 1561 1121 187 110
BsrGI T'GTAC_A
Cuts at: 96 2832 96
Size: 2736 3112
BssHII G'CGCG_C
Cuts at: 1925 2121 1925
Size: 196 5652
BssSI C'ACGA_G
Cuts at: 2751 3908 4920 2751
Size: 1157 1012 3679
Fragments arranged by size:
3679 1157 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2859 794
Size: 2065 3783
BstBI TT'CG_AA
Cuts at: 4652 4652
Size: 5848
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5848
BstXI CCAn_nnnn'nTGG
Cuts at: 2230 2230
Size: 5848
BstYI r'GATC_y
Cuts at: 1972 2271 3178 3545 3930 3947 5399 5485 5497
Size: 299 907 367 385 17 1452 86 12
Cuts at: 5497 5843 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 907 385 367 346 299 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2385 2517 513
Size: 552 1320 132 3844
Fragments arranged by size:
3844 1320 552 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2002 487
Size: 683 56 776 4333
Fragments arranged by size:
4333 776 683 56
BtsI GCAGTG_nn'
Cuts at: 2095 2477 3364 4158 4178 2095
Size: 382 887 794 20 3765
Fragments arranged by size:
3765 887 794 382 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5848
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5813
DraI TTT'AAA
Cuts at: 3152 3152
Size: 5848
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4855 809
Size: 507 3539 1802
Fragments arranged by size:
3539 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2962 3295 4184
Size: 54 1425 50 275 18 1132 333 889
Cuts at: 4184 5813 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1132 889 333 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2962 3295 1537
Size: 275 1150 333 4090
Fragments arranged by size:
4090 1150 333 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3778 1353
Size: 2425 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4435 4809 4955 387
Size: 1064 138 2846 374 146 1280
Fragments arranged by size:
2846 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2010 2282 2010
Size: 272 5576
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 2285 2299 2645 3911 4508 5295
Size: 393 494 159 14 346 1266 597 787
Cuts at: 5295 5563 1239
Size: 268 1524
Fragments arranged by size:
1524 1266 787 597 494 393 346 268
159 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2543 2676 2846 3112 2543
Size: 133 170 266 5279
Fragments arranged by size:
5279 266 170 133
EcoO109I rG'GnC_Cy
Cuts at: 2512 2512
Size: 5848
EcoRI G'AATT_C
Cuts at: 3157 3535 3157
Size: 378 5470
FspI TGC'GCA
Cuts at: 4354 4354
Size: 5848
HaeII r_GCGC'y
Cuts at: 1678 2030 2072 2114 2250 2385 4995 1678
Size: 352 42 42 136 135 2610 2531
Fragments arranged by size:
2610 2531 352 136 135 42 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4489 4521 4563 4595 1373
Size: 32 135 32 2917 32 42 32 2626
Fragments arranged by size:
2917 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2580 3192 3438 669
Size: 988 281 642 612 246 3079
Fragments arranged by size:
3079 988 642 612 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5848
HpaI GTT'AAC
Cuts at: 3438 3438
Size: 5848
KasI G'GCGC_C
Cuts at: 2110 2246 2381 2110
Size: 136 135 5577
Fragments arranged by size:
5577 136 135
KpnI G_GTAC'C
Cuts at: 3173 3173
Size: 5848
MfeI C'AATT_G
Cuts at: 3447 3447
Size: 5848
MluI A'CGCG_T
Cuts at: 5724 5724
Size: 5848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2587 4962 5146 1305
Size: 1282 2375 184 2007
Fragments arranged by size:
2375 2007 1282 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4026
Fragments arranged by size:
4026 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3090 3806 4165 4324 518
Size: 648 1924 716 359 159 2042
Fragments arranged by size:
2042 1924 716 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2087 2983 3012 3264 3941 5089 5334 1606
Size: 481 896 29 252 677 1148 245 2120
Fragments arranged by size:
2120 1148 896 677 481 252 245 29
NarI GG'CG_CC
Cuts at: 2111 2247 2382 2111
Size: 136 135 5577
Fragments arranged by size:
5577 136 135
NcoI C'CATG_G
Cuts at: 513 1065 2517 513
Size: 552 1452 3844
Fragments arranged by size:
3844 1452 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5848
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5848
NotI GC'GGCC_GC
Cuts at: 3295 3295
Size: 5848
NspI r_CATG'y
Cuts at: 1132 1229 2373 3089 3206 4751 1132
Size: 97 1144 716 117 1545 2229
Fragments arranged by size:
2229 1545 1144 716 117 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5848
PciI A'CATG_T
Cuts at: 2369 4747 2369
Size: 2378 3470
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5848
PfoI T'CCnGG_A
Cuts at: 2142 2142
Size: 5848
PmeI GTTT'AAAC
Cuts at: 3152 3152
Size: 5848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2406 2438 3917 3949 5430 5462 2406
Size: 32 1479 32 1481 32 2792
Fragments arranged by size:
2792 1481 1479 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2512 2512
Size: 5848
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5848
PsiI TTA'TAA
Cuts at: 3418 3418
Size: 5848
PstI C_TGCA'G
Cuts at: 830 2198 2279 3200 830
Size: 1368 81 921 3478
Fragments arranged by size:
3478 1368 921 81
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5848
PvuII CAG'CTG
Cuts at: 2983 2983
Size: 5848
SacI G_AGCT'C
Cuts at: 2012 2284 2012
Size: 272 5576
SalI G'TCGA_C
Cuts at: 1936 3190 1936
Size: 1254 4594
SbfI CC_TGCA'GG
Cuts at: 3200 3200
Size: 5848
ScaI AGT'ACT
Cuts at: 2942 4096 5808 2942
Size: 1154 1712 2982
Fragments arranged by size:
2982 1712 1154
SfcI C'TryA_G
Cuts at: 826 1045 2194 2275 3112 3196 4331 5012 5203
Size: 219 1149 81 837 84 1135 681 191
Cuts at: 5203 826
Size: 1471
Fragments arranged by size:
1471 1149 1135 837 681 219 191 84
81
SfoI GGC'GCC
Cuts at: 2112 2248 2383 2112
Size: 136 135 5577
Fragments arranged by size:
5577 136 135
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5848
SmaI CCC'GGG
Cuts at: 1872 3175 1872
Size: 1303 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3952 4853 5115 820
Size: 197 926 2009 901 262 1553
Fragments arranged by size:
2009 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3639 493
Size: 3146 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5848
SphI G_CATG'C
Cuts at: 1132 3089 3206 1132
Size: 1957 117 3774
Fragments arranged by size:
3774 1957 117
SspI AAT'ATT
Cuts at: 5 52 3772 5
Size: 47 3720 2081
Fragments arranged by size:
3720 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5848
StyI C'CwwG_G
Cuts at: 513 1065 2223 2517 2539 3287 513
Size: 552 1158 294 22 748 3074
Fragments arranged by size:
3074 1158 748 552 294 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3875 3892 4045 4230 4675 325
Size: 1255 2295 17 153 185 445 1498
Fragments arranged by size:
2295 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2832 2940 4094
Size: 274 80 33 51 1443 855 108 1154
Cuts at: 4094 5806 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1154 855 274 138 108 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2814 2846 5808 5840 2814
Size: 32 2962 32 2822
Fragments arranged by size:
2962 2822 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5848
XbaI T'CTAG_A
Cuts at: 3184 3184
Size: 5848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2449 2449
Size: 5848
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5848
XmaI C'CCGG_G
Cuts at: 1870 3173 1870
Size: 1303 4545
XmnI GAAnn'nnTTC
Cuts at: 3977 3977
Size: 5848
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3656 276
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SrfI SwaI