Restriction map for
pFN21AB5571
(Circular) (Six-base) MAPSORT of: pFN21AB5571.seq Check: 3767 from: 1 to: 7459
pFN21AB5571 7459 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5269 278
Size: 53 83 186 936 3733 2468
Fragments arranged by size:
3733 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4349 4802 1937
Size: 2412 453 4594
Fragments arranged by size:
4594 2412 453
Acc65I G'GTAC_C
Cuts at: 3428 4780 3428
Size: 1352 6107
AclI AA'CG_TT
Cuts at: 1563 5586 5959 1563
Size: 4023 373 3063
Fragments arranged by size:
4023 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2847 4188 5522 6906 7174 1632
Size: 1215 1341 1334 1384 268 1917
Fragments arranged by size:
1917 1384 1341 1334 1215 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7262
AflIII A'CryG_T
Cuts at: 1203 6358 7335 1203
Size: 5155 977 1327
Fragments arranged by size:
5155 1327 977
AgeI A'CCGG_T
Cuts at: 6290 6290
Size: 7459
AhdI GACnn_n'nnGTC
Cuts at: 6188 6188
Size: 7459
AleI CACnn'nnGTG
Cuts at: 1166 4179 1166
Size: 3013 4446
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7459
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3561 3593 3561
Size: 32 7427
AlwNI CAG_nnn'CTG
Cuts at: 4646 5202 6774 4646
Size: 556 1572 5331
Fragments arranged by size:
5331 1572 556
ApaLI G'TGCA_C
Cuts at: 5516 6672 5516
Size: 1156 6303
ApoI r'AATT_y
Cuts at: 1493 2395 3818 4768 4994 5146 7255 1493
Size: 902 1423 950 226 152 2109 1697
Fragments arranged by size:
2109 1697 1423 950 902 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4161 6013 160
Size: 872 3129 1852 1606
Fragments arranged by size:
3129 1852 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7459
AvaI C'yCGr_G
Cuts at: 1870 1943 4784 1870
Size: 73 2841 4545
Fragments arranged by size:
4545 2841 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2920 2953 2920
Size: 33 7426
BamHI G'GATC_C
Cuts at: 4789 5156 4789
Size: 367 7092
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3428 4780 6235 618
Size: 325 217 627 1641 1352 1455 1842
Fragments arranged by size:
1842 1641 1455 1352 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4778 1391
Size: 3387 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2835 3065 4188 4324 928
Size: 971 936 230 1123 136 4063
Fragments arranged by size:
4063 1123 971 936 230 136
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5650 5684 1903
Size: 34 3713 34 3678
Fragments arranged by size:
3713 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2921 5351 6561 2921
Size: 2430 1210 3819
Fragments arranged by size:
3819 2430 1210
BclI T'GATC_A
Cuts at: 1548 2694 1548
Size: 1146 6313
BfrBI ATG'CAT
Cuts at: 4203 4203
Size: 7459
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6070 136
Size: 107 122 71 631 605 4398 1525
Fragments arranged by size:
4398 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4123 4387 7454 4123
Size: 264 3067 4128
Fragments arranged by size:
4128 3067 264
BlpI GC'TnA_GC
Cuts at: 3883 4876 3883
Size: 993 6466
Bme1580I G_kGCm'C
Cuts at: 1790 5520 6676 1790
Size: 3730 1156 2573
Fragments arranged by size:
3730 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7459
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3991 5190 6148
Size: 416 53 83 399 24 2574 1199 958
Cuts at: 6148 442
Size: 1753
Fragments arranged by size:
2574 1753 1199 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7459
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2094 2364 2953 2962 3480 6119 1239
Size: 855 270 589 9 518 2639 2579
Fragments arranged by size:
2639 2579 855 589 518 270 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3012 3389 3831 4321 5584 6449 6747 3012
Size: 377 442 490 1263 865 298 3724
Fragments arranged by size:
3724 1263 865 490 442 377 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3930 6122 882
Size: 617 2431 2192 2219
Fragments arranged by size:
2431 2219 2192 617
BsaAI yAC'GTr
Cuts at: 493 1204 5250 493
Size: 711 4046 2702
Fragments arranged by size:
4046 2702 711
BsaBI GATnn'nnATC
Cuts at: 3124 3189 7453 3124
Size: 65 4264 3130
Fragments arranged by size:
4264 3130 65
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5266 5648 6283 275
Size: 53 83 186 936 3733 382 635 1451
Fragments arranged by size:
3733 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5892 6290 6564 6711 7122 5892
Size: 398 274 147 411 6229
Fragments arranged by size:
6229 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3052 3082 3478 3508 1178
Size: 30 165 30 1649 30 396 30 5129
Fragments arranged by size:
5129 1649 396 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4005 4388 1186
Size: 2819 383 4257
Fragments arranged by size:
4257 2819 383
BseYI C'CCAG_C
Cuts at: 4353 6662 7158 4353
Size: 2309 496 4654
Fragments arranged by size:
4654 2309 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6886
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4909 5670 6698 1540
Size: 275 186 2908 761 1028 2301
Fragments arranged by size:
2908 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4271 5520 5605 6676 4271
Size: 1249 85 1071 5054
Fragments arranged by size:
5054 1249 1071 85
BsmI GAATG_Cn'
Cuts at: 2614 3299 3887 4970 5063 2614
Size: 685 588 1083 93 5010
Fragments arranged by size:
5010 1083 685 588 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4271 4778 5520 5605 6676 1391
Size: 399 2481 507 742 85 1071 2174
Fragments arranged by size:
2481 2174 1071 742 507 399 85
BspEI T'CCGG_A
Cuts at: 7122 7122
Size: 7459
BspHI T'CATG_A
Cuts at: 5348 7078 5348
Size: 1730 5729
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4814 844
Size: 3970 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4378 5346 1394
Size: 429 2555 968 3507
Fragments arranged by size:
3507 2555 968 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3062 5954 6128 65
Size: 1183 1814 2892 174 1396
Fragments arranged by size:
2892 1814 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6103 6290 1700
Size: 110 4293 187 2869
Fragments arranged by size:
4293 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2116 3043 3765 96
Size: 2020 927 722 3790
Fragments arranged by size:
3790 2020 927 722
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7459
BssSI C'ACGA_G
Cuts at: 3050 5519 6531 3050
Size: 2469 1012 3978
Fragments arranged by size:
3978 2469 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2609 4628 794
Size: 1815 2019 3625
Fragments arranged by size:
3625 2019 1815
BstBI TT'CG_AA
Cuts at: 6263 6263
Size: 7459
BstEII G'GTnAC_C
Cuts at: 1184 4002 1184
Size: 2818 4641
BstXI CCAn_nnnn'nTGG
Cuts at: 3000 3000
Size: 7459
BstYI r'GATC_y
Cuts at: 1972 2080 3013 3289 3973 4123 4340 4387 4586
Size: 108 933 276 684 150 217 47 199
Cuts at: 4586 4789 5156 5541 5558 7010 7096 7108 7454
Size: 203 367 385 17 1452 86 12 346
Cuts at: 7454 1972
Size: 1977
Fragments arranged by size:
1977 1452 933 684 385 367 346 276
217 203 199 150 108 86 47 17
12
Bsu36I CC'TnA_GG
Cuts at: 2229 4755 2229
Size: 2526 4933
BtgI C'CryG_G
Cuts at: 513 1065 4287 513
Size: 552 3222 3685
Fragments arranged by size:
3685 3222 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3422 487
Size: 683 56 2196 4524
Fragments arranged by size:
4524 2196 683 56
BtsI GCAGTG_nn'
Cuts at: 2136 4186 4240 4975 5769 5789 2136
Size: 2050 54 735 794 20 3806
Fragments arranged by size:
3806 2050 794 735 54 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7459
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3035 3070 529
Size: 35 2471 35 4918
Fragments arranged by size:
4918 2471 35 35
DraI TTT'AAA
Cuts at: 2484 4763 2484
Size: 2279 5180
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6466 809
Size: 507 5150 1802
Fragments arranged by size:
5150 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4379 4906 5795
Size: 54 1425 50 275 18 2549 527 889
Cuts at: 5795 7424 8
Size: 1629 43
Fragments arranged by size:
2549 1629 1425 889 527 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4906 1537
Size: 275 3094 4090
Fragments arranged by size:
4090 3094 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3909 5389 1353
Size: 2556 1480 3423
Fragments arranged by size:
3423 2556 1480
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2664 6046 6420 6566 387
Size: 1064 138 1075 3382 374 146 1280
Fragments arranged by size:
3382 1280 1075 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2094 2364 2847 2953 2962 3480 4188
Size: 393 462 270 483 106 9 518 708
Cuts at: 4188 5522 6119 6906 7174 1239
Size: 1334 597 787 268 1524
Fragments arranged by size:
1524 1334 787 708 597 518 483 462
393 270 268 106 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2618 3564 2618
Size: 946 6513
EcoO109I rG'GnC_Cy
Cuts at: 3569 3569
Size: 7459
EcoRI G'AATT_C
Cuts at: 3818 4768 5146 3818
Size: 950 378 6131
Fragments arranged by size:
6131 950 378
FspI TGC'GCA
Cuts at: 5965 5965
Size: 7459
HaeII r_GCGC'y
Cuts at: 1678 6606 1678
Size: 4928 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2696 2728 2822 2854 2966
Size: 32 135 32 1124 32 94 32 112
Cuts at: 2966 2998 3052 3084 3157 3189 4115 4147 4549
Size: 32 54 32 73 32 926 32 402
Cuts at: 4549 4581 6100 6132 6174 6206 1373
Size: 32 1519 32 42 32 2626
Fragments arranged by size:
2626 1519 1124 926 402 135 112 94
73 54 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2010 2581 4803 5049 669
Size: 988 281 72 571 2222 246 3079
Fragments arranged by size:
3079 2222 988 571 281 246 72
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7459
HpaI GTT'AAC
Cuts at: 5049 5049
Size: 7459
KpnI G_GTAC'C
Cuts at: 3432 4784 3432
Size: 1352 6107
MfeI C'AATT_G
Cuts at: 5058 5058
Size: 7459
MluI A'CGCG_T
Cuts at: 7335 7335
Size: 7459
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6573 6757 1305
Size: 5268 184 2007
Fragments arranged by size:
5268 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5637
Fragments arranged by size:
5637 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2184 3869 4179 4410 5417 5776 5935
Size: 648 1018 1685 310 231 1007 359 159
Cuts at: 5935 518
Size: 2042
Fragments arranged by size:
2042 1685 1018 1007 648 359 310 231
159
MspA1I CmG'CkG
Cuts at: 1606 3239 4875 5552 6700 6945 1606
Size: 1633 1636 677 1148 245 2120
Fragments arranged by size:
2120 1636 1633 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6907
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7459
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7459
NotI GC'GGCC_GC
Cuts at: 4906 4906
Size: 7459
NsiI A_TGCA'T
Cuts at: 4205 4205
Size: 7459
NspI r_CATG'y
Cuts at: 1132 1229 3319 4207 4708 4753 4817 6362 1132
Size: 97 2090 888 501 45 64 1545 2229
Fragments arranged by size:
2229 2090 1545 888 501 97 64 45
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7459
PciI A'CATG_T
Cuts at: 6358 6358
Size: 7459
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7459
PmeI GTTT'AAAC
Cuts at: 4763 4763
Size: 7459
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5528 5560 7041 7073 5528
Size: 32 1481 32 5914
Fragments arranged by size:
5914 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3569 3569
Size: 7459
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7459
PsiI TTA'TAA
Cuts at: 2762 5029 2762
Size: 2267 5192
PstI C_TGCA'G
Cuts at: 830 2143 4463 4624 4811 830
Size: 1313 2320 161 187 3478
Fragments arranged by size:
3478 2320 1313 187 161
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7459
PvuII CAG'CTG
Cuts at: 3239 3239
Size: 7459
SalI G'TCGA_C
Cuts at: 1936 4801 1936
Size: 2865 4594
SbfI CC_TGCA'GG
Cuts at: 4811 4811
Size: 7459
ScaI AGT'ACT
Cuts at: 4445 5707 7419 4445
Size: 1262 1712 4485
Fragments arranged by size:
4485 1712 1262
SfcI C'TryA_G
Cuts at: 826 1045 2064 2139 3034 4303 4459 4620 4807
Size: 219 1019 75 895 1269 156 161 187
Cuts at: 4807 5942 6623 6814 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1269 1135 1019 895 681 219 191
187 161 156 75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7459
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7459
SmaI CCC'GGG
Cuts at: 1872 4786 1872
Size: 2914 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3027 3368 3846 4336 5563 6464
Size: 197 926 1084 341 478 490 1227 901
Cuts at: 6464 6726 820
Size: 262 1553
Fragments arranged by size:
1553 1227 1084 926 901 490 478 341
262 197
SnaBI TAC'GTA
Cuts at: 493 5250 493
Size: 4757 2702
SpeI A'CTAG_T
Cuts at: 152 3752 152
Size: 3600 3859
SphI G_CATG'C
Cuts at: 1132 4708 4817 1132
Size: 3576 109 3774
Fragments arranged by size:
3774 3576 109
SspI AAT'ATT
Cuts at: 5 52 2299 5383 5
Size: 47 2247 3084 2081
Fragments arranged by size:
3084 2247 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7459
StyI C'CwwG_G
Cuts at: 513 1065 2614 3341 4019 4898 513
Size: 552 1549 727 678 879 3074
Fragments arranged by size:
3074 1549 879 727 678 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3313 5486 5503 5656 5841 6286 325
Size: 1255 1733 2173 17 153 185 445 1498
Fragments arranged by size:
2173 1733 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2116 3043 3765
Size: 274 80 33 51 1443 139 927 722
Cuts at: 3765 4443 5705 7417 96
Size: 678 1262 1712 138
Fragments arranged by size:
1712 1443 1262 927 722 678 274 139
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7419 7451 7419
Size: 32 7427
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7459
XbaI T'CTAG_A
Cuts at: 2077 4795 2077
Size: 2718 4741
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7459
XmaI C'CCGG_G
Cuts at: 1870 4784 1870
Size: 2914 4545
XmnI GAAnn'nnTTC
Cuts at: 5588 5588
Size: 7459
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5267 276
Size: 53 83 186 936 3733 2468
Fragments arranged by size:
3733 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI