Restriction map for
pFN21AB5569
(Circular) (Six-base) MAPSORT of: pFN21AB5569.seq Check: 1423 from: 1 to: 6112
pFN21AB5569 6112 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3922 278
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3455 1937
Size: 1518 4594
Acc65I G'GTAC_C
Cuts at: 3433 3433
Size: 6112
AclI AA'CG_TT
Cuts at: 1563 4239 4612 1563
Size: 2676 373 3063
Fragments arranged by size:
3063 2676 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2156 2276 2713 4175 5559 5827 1632
Size: 524 120 437 1462 1384 268 1917
Fragments arranged by size:
1917 1462 1384 524 437 268 120
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5915
AflIII A'CryG_T
Cuts at: 1203 5011 5988 1203
Size: 3808 977 1327
Fragments arranged by size:
3808 1327 977
AgeI A'CCGG_T
Cuts at: 4943 4943
Size: 6112
AhdI GACnn_n'nnGTC
Cuts at: 4841 4841
Size: 6112
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3153 1184
Size: 1969 4143
AlwNI CAG_nnn'CTG
Cuts at: 2246 3855 5427 2246
Size: 1609 1572 2931
Fragments arranged by size:
2931 1609 1572
ApaLI G'TGCA_C
Cuts at: 4169 5325 4169
Size: 1156 4956
ApoI r'AATT_y
Cuts at: 1493 2210 3002 3341 3363 3421 3647 3799 5908
Size: 717 792 339 22 58 226 152 2109
Cuts at: 5908 1493
Size: 1697
Fragments arranged by size:
2109 1697 792 717 339 226 152 58
22
AseI AT'TA_AT
Cuts at: 160 1032 4666 160
Size: 872 3634 1606
Fragments arranged by size:
3634 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
AvaI C'yCGr_G
Cuts at: 1870 1943 3437 1870
Size: 73 1494 4545
Fragments arranged by size:
4545 1494 73
BamHI G'GATC_C
Cuts at: 3226 3442 3809 3226
Size: 216 367 5529
Fragments arranged by size:
5529 367 216
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3433 4888 618
Size: 325 217 627 1646 1455 1842
Fragments arranged by size:
1842 1646 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2946 3431 1391
Size: 1555 485 4072
Fragments arranged by size:
4072 1555 485
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5141
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4303 4337 1903
Size: 34 50 34 2282 34 3678
Fragments arranged by size:
3678 2282 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3074 4004 5214 3074
Size: 930 1210 3972
Fragments arranged by size:
3972 1210 930
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6112
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2983 4723 136
Size: 107 122 71 631 605 1311 1740 1525
Fragments arranged by size:
1740 1525 1311 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6107 6107
Size: 6112
BlpI GC'TnA_GC
Cuts at: 3529 3529
Size: 6112
Bme1580I G_kGCm'C
Cuts at: 1790 4173 5329 1790
Size: 2383 1156 2573
Fragments arranged by size:
2573 2383 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6112
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2174 2241 3058
Size: 416 53 83 399 24 757 67 817
Cuts at: 3058 3843 4801 442
Size: 785 958 1753
Fragments arranged by size:
1753 958 817 785 757 416 399 83
67 53 24
BmtI G_CTAG'C
Cuts at: 1056 2750 1056
Size: 1694 4418
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2446 2556 3217 3359 4772 1239
Size: 1207 110 661 142 1413 2579
Fragments arranged by size:
2579 1413 1207 661 142 110
Bpu10I CC'TnA_GC
Cuts at: 2751 2751
Size: 6112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4237 5102 5400 4237
Size: 865 298 4949
Fragments arranged by size:
4949 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2464 4775 882
Size: 617 965 2311 2219
Fragments arranged by size:
2311 2219 965 617
BsaAI yAC'GTr
Cuts at: 493 1204 3903 493
Size: 711 2699 2702
Fragments arranged by size:
2702 2699 711
BsaBI GATnn'nnATC
Cuts at: 6106 6106
Size: 6112
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3919 4301 4936 275
Size: 53 83 186 936 2386 382 635 1451
Fragments arranged by size:
2386 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3024 4545 4943 5217 5364 5775 3024
Size: 1521 398 274 147 411 3361
Fragments arranged by size:
3361 1521 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2158 2188 1178
Size: 30 165 30 755 30 5102
Fragments arranged by size:
5102 755 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6112
BseYI C'CCAG_C
Cuts at: 2065 2328 5315 5811 2065
Size: 263 2987 496 2366
Fragments arranged by size:
2987 2366 496 263
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5539
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3562 4323 5351 1540
Size: 275 186 1561 761 1028 2301
Fragments arranged by size:
2301 1561 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2946 4173 4258 5329 2946
Size: 1227 85 1071 3729
Fragments arranged by size:
3729 1227 1071 85
BsmI GAATG_Cn'
Cuts at: 3623 3716 3623
Size: 93 6019
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2946 3431 4173 4258 5329 1391
Size: 399 1156 485 742 85 1071 2174
Fragments arranged by size:
2174 1156 1071 742 485 399 85
BspEI T'CCGG_A
Cuts at: 5775 5775
Size: 6112
BspHI T'CATG_A
Cuts at: 2481 2656 3294 4001 5731 2481
Size: 175 638 707 1730 2862
Fragments arranged by size:
2862 1730 707 638 175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3467 844
Size: 2623 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3999 1394
Size: 429 2176 3507
Fragments arranged by size:
3507 2176 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4607 4781 65
Size: 1183 3359 174 1396
Fragments arranged by size:
3359 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4756 4943 1700
Size: 110 2946 187 2869
Fragments arranged by size:
2946 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6112
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6112
BssSI C'ACGA_G
Cuts at: 4172 5184 4172
Size: 1012 5100
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6112
BstBI TT'CG_AA
Cuts at: 4916 4916
Size: 6112
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6112
BstXI CCAn_nnnn'nTGG
Cuts at: 2675 2675
Size: 6112
BstYI r'GATC_y
Cuts at: 1972 3226 3442 3809 4194 4211 5663 5749 5761
Size: 1254 216 367 385 17 1452 86 12
Cuts at: 5761 6107 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1254 385 367 346 216 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5560
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5373
Fragments arranged by size:
5373 683 56
BtsI GCAGTG_nn'
Cuts at: 2021 3628 4422 4442 2021
Size: 1607 794 20 3691
Fragments arranged by size:
3691 1607 794 20
ClaI AT'CG_AT
Cuts at: 1554 3135 1554
Size: 1581 4531
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6077
DraI TTT'AAA
Cuts at: 2896 3416 2896
Size: 520 5592
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5119 809
Size: 507 3803 1802
Fragments arranged by size:
3803 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2665 2966 3271
Size: 54 1425 50 275 18 835 301 305
Cuts at: 3271 3559 4448 6077 8
Size: 288 889 1629 43
Fragments arranged by size:
1629 1425 889 835 305 301 288 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3559 1537
Size: 275 1747 4090
Fragments arranged by size:
4090 1747 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3353 4042 1353
Size: 2000 689 3423
Fragments arranged by size:
3423 2000 689
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4699 5073 5219 387
Size: 1064 138 3110 374 146 1280
Fragments arranged by size:
3110 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2944 2944
Size: 6112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2276 2446 2556 2713 3217 3359
Size: 393 524 120 170 110 157 504 142
Cuts at: 3359 4175 4772 5559 5827 1239
Size: 816 597 787 268 1524
Fragments arranged by size:
1524 816 787 597 524 504 393 268
170 157 142 120 110
EcoNI CCTnn'n_nnAGG
Cuts at: 2312 2312
Size: 6112
EcoRI G'AATT_C
Cuts at: 2210 3421 3799 2210
Size: 1211 378 4523
Fragments arranged by size:
4523 1211 378
EcoRV GAT'ATC
Cuts at: 2487 2698 2487
Size: 211 5901
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2869 2869
Size: 6112
FspI TGC'GCA
Cuts at: 2915 4618 2915
Size: 1703 4409
FspAI rTGC'GCAy
Cuts at: 2915 2915
Size: 6112
HaeII r_GCGC'y
Cuts at: 1678 5259 1678
Size: 3581 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3006 3038 4753 4785 4827
Size: 32 135 32 1434 32 1715 32 42
Cuts at: 4827 4859 1373
Size: 32 2626
Fragments arranged by size:
2626 1715 1434 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3456 3702 669
Size: 988 281 1518 246 3079
Fragments arranged by size:
3079 1518 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6112
HpaI GTT'AAC
Cuts at: 3702 3702
Size: 6112
KpnI G_GTAC'C
Cuts at: 3437 3437
Size: 6112
MfeI C'AATT_G
Cuts at: 3711 3711
Size: 6112
MluI A'CGCG_T
Cuts at: 5988 5988
Size: 6112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2443 5226 5410 1305
Size: 1138 2783 184 2007
Fragments arranged by size:
2783 2007 1138 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2968 3273 10
Size: 54 1425 343 1136 305 2849
Fragments arranged by size:
2849 1425 1136 343 305 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2595 3135 4070 4429 4588 518
Size: 648 1429 540 935 359 159 2042
Fragments arranged by size:
2042 1429 935 648 540 359 159
MspA1I CmG'CkG
Cuts at: 1606 3528 4205 5353 5598 1606
Size: 1922 677 1148 245 2120
Fragments arranged by size:
2120 1922 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5560
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6112
NheI G'CTAG_C
Cuts at: 1052 2746 1052
Size: 1694 4418
NotI GC'GGCC_GC
Cuts at: 3559 3559
Size: 6112
NspI r_CATG'y
Cuts at: 1132 1229 2914 3470 5015 1132
Size: 97 1685 556 1545 2229
Fragments arranged by size:
2229 1685 1545 556 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6112
PciI A'CATG_T
Cuts at: 5011 5011
Size: 6112
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2576 1108
Size: 1468 4644
PfoI T'CCnGG_A
Cuts at: 3262 3262
Size: 6112
PmeI GTTT'AAAC
Cuts at: 3416 3416
Size: 6112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4181 4213 5694 5726 4181
Size: 32 1481 32 4567
Fragments arranged by size:
4567 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6112
PsiI TTA'TAA
Cuts at: 3682 3682
Size: 6112
PstI C_TGCA'G
Cuts at: 830 3404 3464 830
Size: 2574 60 3478
Fragments arranged by size:
3478 2574 60
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6112
SacI G_AGCT'C
Cuts at: 2946 2946
Size: 6112
SalI G'TCGA_C
Cuts at: 1936 3454 1936
Size: 1518 4594
SbfI CC_TGCA'GG
Cuts at: 3464 3464
Size: 6112
ScaI AGT'ACT
Cuts at: 3047 4360 6072 3047
Size: 1313 1712 3087
Fragments arranged by size:
3087 1712 1313
SfcI C'TryA_G
Cuts at: 826 1045 2350 3097 3400 3460 4595 5276 5467
Size: 219 1305 747 303 60 1135 681 191
Cuts at: 5467 826
Size: 1471
Fragments arranged by size:
1471 1305 1135 747 681 303 219 191
60
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6112
SmaI CCC'GGG
Cuts at: 1872 3439 1872
Size: 1567 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4216 5117 5379 820
Size: 197 926 2273 901 262 1553
Fragments arranged by size:
2273 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3903 493
Size: 3410 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6112
SphI G_CATG'C
Cuts at: 1132 3470 1132
Size: 2338 3774
SspI AAT'ATT
Cuts at: 5 52 4036 5
Size: 47 3984 2081
Fragments arranged by size:
3984 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6112
StyI C'CwwG_G
Cuts at: 513 1065 2454 3087 3551 513
Size: 552 1389 633 464 3074
Fragments arranged by size:
3074 1389 633 552 464
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4139 4156 4309 4494 4939 325
Size: 1255 2559 17 153 185 445 1498
Fragments arranged by size:
2559 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2631 3045 4358
Size: 274 80 33 51 1443 654 414 1313
Cuts at: 4358 6070 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1313 654 414 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2158 2190 3054 3086 6072 6104 2158
Size: 32 864 32 2986 32 2166
Fragments arranged by size:
2986 2166 864 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2102 1316
Size: 786 5326
XbaI T'CTAG_A
Cuts at: 2074 3448 2074
Size: 1374 4738
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2780 2780
Size: 6112
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6112
XmaI C'CCGG_G
Cuts at: 1870 3437 1870
Size: 1567 4545
XmnI GAAnn'nnTTC
Cuts at: 2414 4241 2414
Size: 1827 4285
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3920 276
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoO109I FseI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI