Restriction map for
pFN21AB5565
(Circular) (Six-base) MAPSORT of: pFN21AB5565.seq Check: 6631 from: 1 to: 6916
pFN21AB5565 6916 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4726 278
Size: 53 83 186 936 3190 2468
Fragments arranged by size:
3190 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2865 4259 1937
Size: 928 1394 4594
Fragments arranged by size:
4594 1394 928
Acc65I G'GTAC_C
Cuts at: 4237 4237
Size: 6916
AclI AA'CG_TT
Cuts at: 1563 5043 5416 1563
Size: 3480 373 3063
Fragments arranged by size:
3480 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3348 3929 4979 6363 6631 1632
Size: 1716 581 1050 1384 268 1917
Fragments arranged by size:
1917 1716 1384 1050 581 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6719
AflIII A'CryG_T
Cuts at: 1203 5815 6792 1203
Size: 4612 977 1327
Fragments arranged by size:
4612 1327 977
AgeI A'CCGG_T
Cuts at: 5747 5747
Size: 6916
AhdI GACnn_n'nnGTC
Cuts at: 5645 5645
Size: 6916
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6916
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6916
AlwNI CAG_nnn'CTG
Cuts at: 2035 4659 6231 2035
Size: 2624 1572 2720
Fragments arranged by size:
2720 2624 1572
ApaLI G'TGCA_C
Cuts at: 2474 4973 6129 2474
Size: 2499 1156 3261
Fragments arranged by size:
3261 2499 1156
ApoI r'AATT_y
Cuts at: 1493 2387 2430 2497 2655 2699 2852 3216 3536
Size: 894 43 67 158 44 153 364 320
Cuts at: 3536 3952 4076 4225 4451 4603 6712 1493
Size: 416 124 149 226 152 2109 1697
Fragments arranged by size:
2109 1697 894 416 364 320 226 158
153 152 149 124 67 44 43
AseI AT'TA_AT
Cuts at: 160 1032 2247 2279 2760 5470 160
Size: 872 1215 32 481 2710 1606
Fragments arranged by size:
2710 1606 1215 872 481 32
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6916
AvaI C'yCGr_G
Cuts at: 1870 1943 2098 4241 1870
Size: 73 155 2143 4545
Fragments arranged by size:
4545 2143 155 73
BamHI G'GATC_C
Cuts at: 3466 4246 4613 3466
Size: 780 367 5769
Fragments arranged by size:
5769 780 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4237 5692 618
Size: 325 217 627 2450 1455 1842
Fragments arranged by size:
2450 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4235 1391
Size: 2844 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4059 928
Size: 971 2160 3785
Fragments arranged by size:
3785 2160 971
BbvCI CC'TCA_GC
Cuts at: 2028 2028
Size: 6916
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2855 2889 5107 5141 1903
Size: 34 918 34 2218 34 3678
Fragments arranged by size:
3678 2218 918 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2475 3305 4808 6018 2475
Size: 830 1503 1210 3373
Fragments arranged by size:
3373 1503 1210 830
BclI T'GATC_A
Cuts at: 1548 2308 2575 1548
Size: 760 267 5889
Fragments arranged by size:
5889 760 267
BfrBI ATG'CAT
Cuts at: 4132 4132
Size: 6916
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5527 136
Size: 107 122 71 631 605 3855 1525
Fragments arranged by size:
3855 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6911 6911
Size: 6916
BlpI GC'TnA_GC
Cuts at: 3054 4333 3054
Size: 1279 5637
Bme1580I G_kGCm'C
Cuts at: 1790 2478 4977 6133 1790
Size: 688 2499 1156 2573
Fragments arranged by size:
2573 2499 1156 688
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6916
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4647 5605 442
Size: 416 53 83 399 24 3230 958 1753
Fragments arranged by size:
3230 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6916
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2113 2113
Size: 6916
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2543 3878 4141 5576 1239
Size: 1304 1335 263 1435 2579
Fragments arranged by size:
2579 1435 1335 1304 263
Bpu10I CC'TnA_GC
Cuts at: 2028 2028
Size: 6916
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5041 5906 6204 5041
Size: 865 298 5753
Fragments arranged by size:
5753 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5579 882
Size: 617 4080 2219
Fragments arranged by size:
4080 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4707 493
Size: 711 3503 2702
Fragments arranged by size:
3503 2702 711
BsaBI GATnn'nnATC
Cuts at: 2574 2886 6910 2574
Size: 312 4024 2580
Fragments arranged by size:
4024 2580 312
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4723 5105 5740 275
Size: 53 83 186 936 3190 382 635 1451
Fragments arranged by size:
3190 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5349 5747 6021 6168 6579 5349
Size: 398 274 147 411 5686
Fragments arranged by size:
5686 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2221 2251 1178
Size: 30 165 30 818 30 5843
Fragments arranged by size:
5843 818 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3240 1186
Size: 2054 4862
BseYI C'CCAG_C
Cuts at: 6119 6615 6119
Size: 496 6420
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2824 1163
Size: 573 1088 5255
Fragments arranged by size:
5255 1088 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4366 5127 6155 1540
Size: 275 186 2365 761 1028 2301
Fragments arranged by size:
2365 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2478 4977 5062 6133 2478
Size: 2499 85 1071 3261
Fragments arranged by size:
3261 2499 1071 85
BsmI GAATG_Cn'
Cuts at: 2345 4427 4520 2345
Size: 2082 93 4741
Fragments arranged by size:
4741 2082 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2478 4235 4977 5062 6133 1391
Size: 399 688 1757 742 85 1071 2174
Fragments arranged by size:
2174 1757 1071 742 688 399 85
BspEI T'CCGG_A
Cuts at: 6579 6579
Size: 6916
BspHI T'CATG_A
Cuts at: 3019 3787 4003 4805 6535 3019
Size: 768 216 802 1730 3400
Fragments arranged by size:
3400 1730 802 768 216
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4271 844
Size: 3427 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4803 1394
Size: 429 2980 3507
Fragments arranged by size:
3507 2980 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3243 3891 4029 4191 5411 5585 65
Size: 1183 1995 648 138 162 1220 174 1396
Fragments arranged by size:
1995 1396 1220 1183 648 174 162 138
BsrFI r'CCGG_y
Cuts at: 1700 1810 5560 5747 1700
Size: 110 3750 187 2869
Fragments arranged by size:
3750 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3516 96
Size: 3420 3496
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6916
BssSI C'ACGA_G
Cuts at: 4976 5988 4976
Size: 1012 5904
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6916
BstBI TT'CG_AA
Cuts at: 5720 5720
Size: 6916
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6916
BstXI CCAn_nnnn'nTGG
Cuts at: 3124 3124
Size: 6916
BstYI r'GATC_y
Cuts at: 1972 3466 4210 4246 4613 4998 5015 6467 6553
Size: 1494 744 36 367 385 17 1452 86
Cuts at: 6553 6565 6911 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1494 1452 744 385 367 346 86
36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5425
Fragments arranged by size:
5425 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6177
Fragments arranged by size:
6177 683 56
BtsI GCAGTG_nn'
Cuts at: 3555 4203 4432 5226 5246 3555
Size: 648 229 794 20 5225
Fragments arranged by size:
5225 794 648 229 20
ClaI AT'CG_AT
Cuts at: 1554 2164 1554
Size: 610 6306
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6881
DraI TTT'AAA
Cuts at: 2697 3213 4220 2697
Size: 516 1007 5393
Fragments arranged by size:
5393 1007 516
DraIII CAC_nnn'GTG
Cuts at: 2410 2410
Size: 6916
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5923 809
Size: 507 4607 1802
Fragments arranged by size:
4607 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2438 4363 5252
Size: 54 1425 50 275 18 608 1925 889
Cuts at: 5252 6881 8
Size: 1629 43
Fragments arranged by size:
1925 1629 1425 889 608 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4363 1537
Size: 275 2551 4090
Fragments arranged by size:
4090 2551 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3996 4846 1353
Size: 2643 850 3423
Fragments arranged by size:
3423 2643 850
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5503 5877 6023 387
Size: 1064 138 3914 374 146 1280
Fragments arranged by size:
3914 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2543 3348 3878 3929 4141 4979 5576
Size: 393 911 805 530 51 212 838 597
Cuts at: 5576 6363 6631 1239
Size: 787 268 1524
Fragments arranged by size:
1524 911 838 805 787 597 530 393
268 212 51
EcoO109I rG'GnC_Cy
Cuts at: 2206 2206
Size: 6916
EcoRI G'AATT_C
Cuts at: 2497 2852 4225 4603 2497
Size: 355 1373 378 4810
Fragments arranged by size:
4810 1373 378 355
EcoRV GAT'ATC
Cuts at: 3835 3835
Size: 6916
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3002 3002
Size: 6916
FspI TGC'GCA
Cuts at: 5422 5422
Size: 6916
HaeII r_GCGC'y
Cuts at: 1678 2048 6063 1678
Size: 370 4015 2531
Fragments arranged by size:
4015 2531 370
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3232 3264 5557 5589 5631
Size: 32 135 32 1660 32 2293 32 42
Cuts at: 5631 5663 1373
Size: 32 2626
Fragments arranged by size:
2626 2293 1660 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2533 2866 2961 4260 4506 669
Size: 988 281 595 333 95 1299 246 3079
Fragments arranged by size:
3079 1299 988 595 333 281 246 95
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6916
HpaI GTT'AAC
Cuts at: 4506 4506
Size: 6916
KpnI G_GTAC'C
Cuts at: 4241 4241
Size: 6916
MfeI C'AATT_G
Cuts at: 4090 4515 4090
Size: 425 6491
MluI A'CGCG_T
Cuts at: 6792 6792
Size: 6916
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6030 6214 1305
Size: 4725 184 2007
Fragments arranged by size:
4725 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2440 10
Size: 54 1425 343 608 4486
Fragments arranged by size:
4486 1425 608 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3128 4005 4155 4196 4874 5233 5392
Size: 648 1962 877 150 41 678 359 159
Cuts at: 5392 518
Size: 2042
Fragments arranged by size:
2042 1962 877 678 648 359 159 150
41
MspA1I CmG'CkG
Cuts at: 1606 2032 4332 5009 6157 6402 1606
Size: 426 2300 677 1148 245 2120
Fragments arranged by size:
2300 2120 1148 677 426 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5425
Fragments arranged by size:
5425 939 552
NdeI CA'TA_TG
Cuts at: 387 2527 387
Size: 2140 4776
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6916
NotI GC'GGCC_GC
Cuts at: 4363 4363
Size: 6916
NsiI A_TGCA'T
Cuts at: 4134 4134
Size: 6916
NspI r_CATG'y
Cuts at: 1132 1229 4274 5819 1132
Size: 97 3045 1545 2229
Fragments arranged by size:
3045 2229 1545 97
PacI TTA_AT'TAA
Cuts at: 3405 3405
Size: 6916
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6916
PciI A'CATG_T
Cuts at: 5815 5815
Size: 6916
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2410 2820 3288 1108
Size: 1302 410 468 4736
Fragments arranged by size:
4736 1302 468 410
PfoI T'CCnGG_A
Cuts at: 2559 3882 2559
Size: 1323 5593
PmeI GTTT'AAAC
Cuts at: 4220 4220
Size: 6916
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4985 5017 6498 6530 4985
Size: 32 1481 32 5371
Fragments arranged by size:
5371 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2206 2206
Size: 6916
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6916
PsiI TTA'TAA
Cuts at: 3582 4486 3582
Size: 904 6012
PspXI vC'TCGA_Gb
Cuts at: 2098 2098
Size: 6916
PstI C_TGCA'G
Cuts at: 830 2878 4268 830
Size: 2048 1390 3478
Fragments arranged by size:
3478 2048 1390
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6916
PvuII CAG'CTG
Cuts at: 2032 2032
Size: 6916
SalI G'TCGA_C
Cuts at: 1936 2864 4258 1936
Size: 928 1394 4594
Fragments arranged by size:
4594 1394 928
SbfI CC_TGCA'GG
Cuts at: 4268 4268
Size: 6916
ScaI AGT'ACT
Cuts at: 5164 6876 5164
Size: 1712 5204
SfcI C'TryA_G
Cuts at: 826 1045 2133 2679 2874 4264 5399 6080 6271
Size: 219 1088 546 195 1390 1135 681 191
Cuts at: 6271 826
Size: 1471
Fragments arranged by size:
1471 1390 1135 1088 681 546 219 195
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6916
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6916
SmaI CCC'GGG
Cuts at: 1872 4243 1872
Size: 2371 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2098 5020 5921 6183 820
Size: 197 926 155 2922 901 262 1553
Fragments arranged by size:
2922 1553 926 901 262 197 155
SnaBI TAC'GTA
Cuts at: 493 4707 493
Size: 4214 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6916
SphI G_CATG'C
Cuts at: 1132 4274 1132
Size: 3142 3774
SspI AAT'ATT
Cuts at: 5 52 2186 4840 5
Size: 47 2134 2654 2081
Fragments arranged by size:
2654 2134 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6916
StyI C'CwwG_G
Cuts at: 513 1065 2004 2209 3203 4355 513
Size: 552 939 205 994 1152 3074
Fragments arranged by size:
3074 1152 994 939 552 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2066 3494 4943 4960 5113 5298 5743
Size: 1255 486 1428 1449 17 153 185 445
Cuts at: 5743 325
Size: 1498
Fragments arranged by size:
1498 1449 1428 1255 486 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3396 3516 5162
Size: 274 80 33 51 1443 1419 120 1646
Cuts at: 5162 6874 96
Size: 1712 138
Fragments arranged by size:
1712 1646 1443 1419 274 138 120 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2004 2036 2221 2253 3140 3172 6876 6908 2004
Size: 32 185 32 887 32 3704 32 2012
Fragments arranged by size:
3704 2012 887 185 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6916
XbaI T'CTAG_A
Cuts at: 4252 4252
Size: 6916
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2669 3699 4116 2669
Size: 1030 417 5469
Fragments arranged by size:
5469 1030 417
XhoI C'TCGA_G
Cuts at: 1943 2098 1943
Size: 155 6761
XmaI C'CCGG_G
Cuts at: 1870 4241 1870
Size: 2371 4545
XmnI GAAnn'nnTTC
Cuts at: 2376 4056 4164 5045 2376
Size: 1680 108 881 4247
Fragments arranged by size:
4247 1680 881 108
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4724 276
Size: 53 83 186 936 3190 2468
Fragments arranged by size:
3190 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI PmlI PspOMI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI